The document discusses intracellular and extracellular accumulation in various organs. It describes cases of glycogen accumulation seen as vacuolation in hepatocyte cytoplasm on H&E staining of the liver. Kidney samples show intracellular hyaline droplets and extracellular hyaline occupying renal tubules. Amyloidosis is seen in the pancreas, kidney, lymph node, duodenum and heart, appearing as extracellular deposition of amorphous eosinophilic material that stains pink with Congo Red.

1. Intracellular and Extracellular
accumulation
Dr. Azad Kareem Saeed
Histopathology Department
15/10/2012

2. A B
Organ: liver
Lesions:
A: liver is enlarged, pale brown to beige, edges rounded and
the cut surface is not be greasy.
B: The hepatocytes are distended (arrows) with extensive
cytoplasmic vacuolation. H&E stain.
Dx: Glycogen accumulation.

3. Organ: liver
Lesions:
A: Presence of clear, empty
vacuole within hepatocyte
A
cytoplasm.
B: Periodic acid-Schiff reaction
shows irregular distribution of
glycogen (darker color) in the
hepatocytes.
Dx: Glycogen accumulation
B

4. Organ: Liver
Lesions:
Periodic acid-Schiff stain
shows irregular distribution of
glycogen (violet color) in the
hepatocytes.
Dx: Glycogen accumulation

5. Extracellular accumulation

6. Organ:
Kidney
Lesions: 1. Presence of homogenous, glassy, eosinophilic substances
within the cytoplasm of epithelial cells (Blue arrows).
2. Homogenous, glassy, eosinophilic substances occupied the lumen of
renal tubule (black arrow).
Dx: 1. Intracellular hyaline (Hyaline droplet).
2. Extracellular hyaline.

7. Organ: Kidney
Lesions:
The wall of the afferent glomerular arteriole appears as a
glassy pink, homogenous deposit (blue arrow).
Dx: Extracellular hyaline.

8. Amyloidosis

9. Organ:
Pancreas
Lesion:
Deposition of an
amorphous,
homogenous,
eosinophilic, material
(arrow) extracellularly
within the glandular
tissue.
Diagnosis: Amyloidosis

10. Organ: Kidney
Lesion: The glomerular architecture is nearly totally obliterated
by amorphus pink material.
Dx: Glomerular Amyloidosis

11. Organ: Lymph node
Lesion:
Deposition of an amorphous, homogenous, eosinophilic,
material (arrows) extracellularly within the lymphatic
tissue.
Dx: Amyloidosis (Right: H&E, Left: Congo red).

12. Organ: A
duodenum
Lesions:
A: Presence of amorphous
homogenous pink material
in lamina propria (H&E)
and around blood vessels.
B: an amorphous
homogenous bright pink
material in lamina propria
and around blood vessels
after staining with Congo
red.
Dx: Amyloidosis B

13. Organ: Heart
Lesions:
A: Presence of extracellular
amorphous homogenous
pink material (Blue arrow). A
B: Same section stained
with congo red, the amyloid
protein appeared as bright
pink (black arrow).
Dx: Amyloidosis
B