Crystallography is the study of crystalline solids, their external shape, internal structure, and the principles governing their growth. It can be divided into geometric, physical, and chemical sections. X-ray crystallography uses the diffraction of X-rays hitting crystallized molecules to determine their structure. Crystals are classified based on their degree of crystallization as euhedral, subhedral, or anhedral. Protein crystallography involves obtaining and purifying proteins, crystallizing them, collecting diffraction data through X-ray experiments, and determining the protein structure through phasing, modeling, and refinement.

1. SUB: APPLIED BIO CHEMISTRY
ABINAYA.P

2.  CRYSTALLOGRAPHY - It is simply a funcy
word meaning “ THE STUDY OF CRYSTALS”
 The study of crystalline soilds and the
principle that govern their growth, external
shape, and internal structure.
 Crystallography is easily divided into 3
sections geometrical, physical, and chemical.

3.  X-ray crystallography uses the uniformity of
light diffraction of crystals to determine the
structure of a molecule or atom.
 Then they use an X-ray beam to “HIT” the
crystallized molecules.
 The electrons surrounding the molecules
diffract as the X-rays hit them.
 This forms a pattern, this types of pattern is
called the X-ray diffraction pattern.

4.  Classification of crystals according to the
degree of crystallization.
1. EUHEDRAL CRYSTALS
2. SUBHEDRAL
3.ANHEDRAL CRYSTALS

6.  Crystal Properties, Space groups
 Diffraction
 Bragg’s Law, von Laue condition
 X-RAY diffraction data collection

7.  OBTAINING PROTEIN;
[e.g. Molecular Cloning and Expression]
 PROTEIN PURIFICATION;
[e.g. Fast protein liquid
Chromatography]
 PROTEIN CRYSTALLIZATION;
[e.g. Vapor diffusion technique]
 DATA COLLECTION;
[e.g. X-rays diffraction experiment]
 STRUCTURE DETERMINATION;
[e.g. Phasing, model building and
refinement]

8. THE END