Antigen is any substance that induces an immune response in the body. There are two main types: complete antigens that can induce an immune response on their own, and incomplete antigens or haptens that require a carrier molecule to become immunogenic. Antigens are recognized by immune cells through antigen determinants or epitopes. For a response, antigens must be processed and presented by antigen-presenting cells to be recognized by T cells through MHC molecules. The properties of an antigen like its size, structure, and route of administration influence its ability to induce an immune response.
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
An antigen is either a cell or molecule which will bind with pre exiting antibody but will not definitely cause induction of antibody production. Antigen may also be defined as ‘a macro molecular entity that essentially elicits an immune response via the formation of specific antibodies in the body of the host’. And Hapten may also be defined ‘as a substance that normally does not act as an antigen or stimulate an immune response but that can be combined with an antigen and, at a later time, initiate a specific antibody response on its own’.
Altering the specificity of T cell receptor (TCR) is one of the popular strategies to genetically modify T cells to enhance the tumor-killing activity of T cells. From a tumor-reactive T cell or active anti-tumor T-cell antigens, the appropriate target sequence is introduced to modify T cells to target a broad range of tumors with improved specificity. https://www.creative-biolabs.com/car-t/cellrapeutics-tcr-technology.htm
T-Cell Activation
• Concept of immune response
• T cell-mediated immune response
• B cell-mediated immune response
I. Concept of immune response
• A collective and coordinated response to the introduction of foreign substances in an individual mediated by the cells and molecules in the immune system.
II. T cell-mediated immune response
• Cell-mediated immunity is the arm of the adaptive immune response whose role is to combat infection of intracellular pathogens, such as intracellular bacteria (mycobacteria, listeria monocytogens), viruses, protozoa, etc.
A DNA vaccine is a type of vaccine that transfects a specific antigen-coding DNA sequence into the cells of an organism as a mechanism to induce an immune response.
DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune response is sought, so the cells directly produce the antigen, thus causing a protective immunological response.
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
An antigen is either a cell or molecule which will bind with pre exiting antibody but will not definitely cause induction of antibody production. Antigen may also be defined as ‘a macro molecular entity that essentially elicits an immune response via the formation of specific antibodies in the body of the host’. And Hapten may also be defined ‘as a substance that normally does not act as an antigen or stimulate an immune response but that can be combined with an antigen and, at a later time, initiate a specific antibody response on its own’.
Altering the specificity of T cell receptor (TCR) is one of the popular strategies to genetically modify T cells to enhance the tumor-killing activity of T cells. From a tumor-reactive T cell or active anti-tumor T-cell antigens, the appropriate target sequence is introduced to modify T cells to target a broad range of tumors with improved specificity. https://www.creative-biolabs.com/car-t/cellrapeutics-tcr-technology.htm
T-Cell Activation
• Concept of immune response
• T cell-mediated immune response
• B cell-mediated immune response
I. Concept of immune response
• A collective and coordinated response to the introduction of foreign substances in an individual mediated by the cells and molecules in the immune system.
II. T cell-mediated immune response
• Cell-mediated immunity is the arm of the adaptive immune response whose role is to combat infection of intracellular pathogens, such as intracellular bacteria (mycobacteria, listeria monocytogens), viruses, protozoa, etc.
A DNA vaccine is a type of vaccine that transfects a specific antigen-coding DNA sequence into the cells of an organism as a mechanism to induce an immune response.
DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune response is sought, so the cells directly produce the antigen, thus causing a protective immunological response.
What is antigen
What is epitope & paratope?
Classification of antigen
Pro antigen
Superantigens
Antigenicity
Determinants of antigenicity
Test for antigen detection
Here are five things to know about coronavirus tests: PCR and antigen tests are the most common but they work differently. While antigen tests look for proteins ...
An antigen is any substance that causes your immune system to produce antibodies against it. This means your immune system does not recognize the substance, and is trying to fight it off. An antigen may be a substance from th
The general objectives of involving the public at different stages in the EIA process were considered by a recent European Commission research project (EC, 1999) as given in the table.
European union is composed of several hundred countries including Muslim nations like Turkey, Albania etc. So, if one gets access into nation like Poland or Romania, he gets access throughout Europe.
Objectives of public participation during stages of the EIA process
Formal opportunities for public participation in EIA are defined in legislation. While rights of involvement in many countries are limited to opportunities for viewing and commenting on finalised reports, in principle, public consultation and participation can occur at every stage in the EIA process. The table summarizes the main objectives of public involvement at each stage of the EIA process, including a detailed description of these objectives (EC, 1999).
In brief, literature shows that there are a number of advantages of involving the public early on in the EIA process. If participation does occur early on then interaction between the public, developer and decision-making body should continue throughout the EIA process if the full benefits are to be seen.
Table : Summary of Objectives of Public Involvement in EIA
Stage of EIA process Objectives of public involvement
Screening
Identification of significant impacts
Scoping
• Identification of public's interest and values
• Identification of priorities for assessment
• Encouraging public understanding of the proposed project
Assessment
• The public can contribute local knowledge and values to the prediction, evaluation and mitigation of impacts
• Improvement in quality and acceptability of EIA report
EIA Report Review
Public contribute to evaluation of quality and acceptability of report
Decision
Public comment on acceptability of project impacts
Monitoring
Public evaluate impacts that occur and support project environmental management process
There is an international agency called Environmental Protection Agency EPA. EPA forms and implements regulations regarding making the environment better.
We are at present in a very critical state as far as the situation of global warming and receding snowcaps in the Antarctica. It has been found that there is a hole in the Ozone layer due to increasing level of pollution throughout the world.
The rising sea-levels are threatening the global community. Moreover, the increasing amount of garbage thrown into the seas is a major problem now for countries like Canada. How to manage this sort of problems is equally challenging in both the developed and underdeveloped nations.
6. Solution mitigation of climate change.pptxNeeraj Ojha
There is an international agency called Environmental Protection Agency EPA. EPA forms and implements regulations regarding making the environment better.
We are at present in a very critical state as far as the situation of global warming and receding snowcaps in the Antarctia. It has been found that there is a hole in the Ozone layer due to increasing level of pollution throughout the world.
5.Climate change and its impact on environment.pptxNeeraj Ojha
There is an international agency called Environmental Protection Agency EPA. EPA forms and implements regulations regarding making the environment better.
We are at present in a very critical state as far as the situation of global warming and receding snowcaps in the Antarctia. It has been found that there is a hole in the Ozone layer due to increasing level of pollution throughout the world.
4BDirect and indirect health effects of climate change.pptxNeeraj Ojha
As far as Nepalese people are concerned, they are very bad in their food habits. Disease like ulcer and diabetes are rampant along Nepalese people. Moreover, there are areas in the country where there is a severe malnutrition.
Factors influencing food habits
•Individual Preferences
Every individual has unique likes and dislikes concerning foods.
•Cultural Influences
A cultural group provides guidelines regarding acceptable foods, food combinations, eating patterns, and eating behaviors.
•Social Influences
Members of asocial group depend on each other, share a common culture, and influence each other's behaviors and values.
As far as Nepalese people are concerned, they are very bad in their food habits. Disease like ulcer and diabetes are rampant along Nepalese people. Moreover, there are areas in the country where there is a severe malnutrition.
Factors influencing food habits
•Individual Preferences
Every individual has unique likes and dislikes concerning foods.
•Cultural Influences
A cultural group provides guidelines regarding acceptable foods, food combinations, eating patterns, and eating behaviors.
•Social Influences
Members of asocial group depend on each other, share a common culture, and influence each other's behaviors and values.
2,3 Greenhouse gases, global scenario, green house effectt and global warming...Neeraj Ojha
As far as Nepalese people are concerned, they are very bad in their food habits. Disease like ulcer and diabetes are rampant along Nepalese people. Moreover, there are areas in the country where there is a severe malnutrition.
Factors influencing food habits
•Individual Preferences
Every individual has unique likes and dislikes concerning foods.
•Cultural Influences
A cultural group provides guidelines regarding acceptable foods, food combinations, eating patterns, and eating behaviors.
•Social Influences
Members of asocial group depend on each other, share a common culture, and influence each other's behaviors and values.
8.1Determinants of Adaptive Capacity.pptxNeeraj Ojha
There is an international agency called Environmental Protection Agency EPA. EPA forms and implements regulations regarding making the environment better.
We are at present in a very critical state as far as the situation of global warming and receding snowcaps in the Antarctia. It has been found that there is a hole in the Ozone layer due to increasing level of pollution throughout the world.
The rising sea-levels are threatening the global community. Moreover, the increasing amount of garbages thrown into the seas is a major problem now for countries like Canada. How to manage this sort of problems is equally challenging in both the developed and underdeveloped nations.
There is an international agency called Environmental Protection Agency EPA. EPA forms and implements regulations regarding making the environment better.
We are at present in a very critical state as far as the situation of global warming and receding snowcaps in the Antarctia. It has been found that there is a hole in the Ozone layer due to increasing level of pollution throughout the world.
The rising sea-levels are threatening the global community. Moreover, the increasing amount of garbages thrown into the seas is a major problem now for countries like Canada. How to manage this sort of problems is equally challenging in both the developed and underdeveloped nations.
4A Socio-economic and environmental impacts of climate change.pptxNeeraj Ojha
As far as Nepalese people are concerned, they are very bad in their food habits. Disease like ulcer and diabetes are rampant along Nepalese people. Moreover, there are areas in the country where there is a severe malnutrition.
Factors influencing food habits
•Individual Preferences
Every individual has unique likes and dislikes concerning foods.
•Cultural Influences
A cultural group provides guidelines regarding acceptable foods, food combinations, eating patterns, and eating behaviors.
•Social Influences
Members of asocial group depend on each other, share a common culture, and influence each other's behaviors and values.
Eating disorders are complex, bio-psycho-social conditions, with multiple causes. Eating disorders arise from a combination of established social, psychological, biological, and interpersonal factors. While they may begin with preoccupations with food and weight, they are most often about much more than food. It is important to understand that the factors that contribute to eating disorders are complex and multifaceted; they are not simply about weight and they are certainly not choices.
अमेरिकामा नेपाली मामिलामा सीबीआईको अनुसन्धान
https://cib.nepalpolice.gov.np/
पछिल्लो समय अमेरिकाको मूलधारको राजनीतिमा नेपाली मूलका थोरै मानिसहरु प्रवेश गर्न सफल भएका छन् । मेरिल्याण्डका प्राध्यापक ह्यारी भण्डारी( Harry Bhandari, Maryland) डेमोक्रेटिक ककसमा प्रवेश गरेका छन् । त्यस्तै दर्शन रौनियार (Darshan Rauniyar, California) अमेरिकी सिनेटमा उम्मेदवार बनेका छन् । उहाँसँग धेरै सहयोग र पैसा भए पनि, एक नाबालिगद्वारा बलात्कारको आरोपमा उहाँलाई ककसमा अवरुद्ध गरियो।
अमेरिकामा प्रचण्ड र कम्युनिस्ट साम्राज्य न्यूयोर्कदेखि टेक्साससम्म फैलिएको छ । उनीहरुले पासाङ लामा, कुन्ती थापा, श्रुति कंसाकार(Euless Texas), सिर्जना मुनिकार(Jerseycity, NJ) , साङ शेर्पा(Construction), विजय पौडेल (Journalism) लगायत अमेरिकामा रहेका आफ्ना एजेन्ट र व्यक्तिसँग चन्दा उठाएर अर्बौं डलरको पैसा लुटेका छन् । श्रीलंकाको तमिल टाइगर्स र भारतीय माओवादीहरू: अष्ट्रेलिया, अमेरिका, युनाइटेड किंगडम र क्यानडा जस्ता विकसित राष्ट्रहरूमा तिनीहरूको धेरै ठूलो सञ्चालन छ।बालकुमारीमा बस्ने माधव नेपालजस्ता मानिसहरूलाई यी कम्युनिष्ट डन र गुण्डाहरूले धेरै रक्षा गर्न सक्छन्। धेरै कांग्रेसका कार्यकर्ताहरू प्रलोभनमा पारेर अमेरिकामा पैसा लुट्दै हत्या वा आत्महत्या जस्तो देखिने गरी हत्या हुन्छ। प्रचण्डको कारबाही क्लिन्टन फाउण्डेशन (Clinton Foundation) , वर्तमान राष्ट्रपति बाइडेन र अमेरिकामा सक्रिय जासुससँग जोडिएको छ ।
चिनियाँहरूले क्वीन्समा 5339 97st कोरोना, NY जस्ता धेरै घरहरू ल्याएका छन् जहाँ उनीहरूले कांग्रेस र राप्रपा कार्यकर्ताहरूलाई प्रलोभनमा पारेका छन् र उनीहरूलाई संयुक्त राज्यको डेमोक्रेटिक पार्टीको सहयोगमा थुनेका छन्। अमेरिकामा धेरै वरिष्ठ राजतन्त्र समर्थक र पूर्व सेना सदस्यहरू(eg. Bishwa Bahadur Shah, Bishal Bikram Shah, Kamala Prasai ) यस तरिकाले मारिएका छन् वा विषाक्त छन्। रञ्जित थापा र बुद्ध बस्ताकोटी जस्ता पूर्वप्रहरी पनि यी अपरेशनमा संलग्न रहेको पाइएको छ ।
यो समग्र परिदृश्यलाई हेर्दा पछिल्लो समयको विकासको सन्दर्भमा नेपालको संविधानमा परिवर्तन र संशोधन गर्नुपर्ने आवश्यकता पनि हामीले देखेका छौं । हामीले लामो समयदेखि अल्पसंख्यक जनसंख्या र गे-लेस्बियन-ट्रान्सजेन्डर (LGBT) समुदायको शक्तिलाई बेवास्ता गर्दै आएका छौं। यही कारणले उनीहरू विदेशी शक्तिसँग गठबन्धन गर्दै राष्ट्रिय सुरक्षाका (national security) लागि खतरा बनिरहेका छन् । विशेषगरी इजरायल जस्ता देशमा सिआइएको कारबाही विश्वका लागि खतरा बन्दै गएको छ । सीआईएको इजरायल राष्ट्रमा ठूलो भूमिगत आणविक सुविधा (underground nuclear weapon facility) छ जुन हाम्रो देशको धेरै नजिक छ। यसको मतलब यो हो कि सानो द्वन्द्वको अवस्थामा सीआईएले(CIA) आफ्नो आणविक शक्ति सिधै नेपालमा घुमाउन सक्छ। तसर्थ, हामी आजदेखि नै होसियार हुनुपर्दछ र अन्य सहयोगीहरूलाई पनि त्यस्तै गर्न चेतावनी दिनु पर्छ।
लिन्डन बी जोनसनले जेएफकेको (JFK) हत्यामा इटालियन माफिया (Organized Crime ) सँग सहयोग गरेको भनिन्छ। माफियाहरूले आफ्नो व्यापार र प्रभाव बढाउन तत्कालीन कम्युनिस्ट भियतनामसँग युद्ध गर्न चाहन्थे। ठ्याक्कै परिस्थिति अहिले हामीले सामना गरिरहेका छौं। अमेरिकाको लुटेरा टोली हाम्रो राष्ट्रसँग युद्ध चाहन्छ र उनीहरूले बाइडेन प्रशासनलाई भारतसँग मिलेर राजधानी काठमाडौंमा आक्रमण गर्न बाध्य पारिरहेका छन् । मोदी सरकारले सधैं नेपाललाई आफ्नो क्षेत्रको रूपमा हावी गर्न खोजेको छ। यसै कारण मनमोहन सिंहको प्रधानमन्त्र
PHG 481 Traditional Systems of Medicine BPHARM syllabus PUNeeraj Ojha
Traditional system of Medicine includes Chinese medicine, Ayurveda, Tibetan medicine, Kampo, Homeopathy etc. Answers are derived from teacher's notes and internet
Pht 402 Clinical Pharmacy questions answered OLD IS GOLDNeeraj Ojha
These Q/A's are based upon the syllabus by Pokhara University based in the city of Pokhara, Nepal. Hope this will be helpful to the beginners in the schools of pharmacy.
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Diabetes is a rapidly and serious health problem in Pakistan. This chronic condition is associated with serious long-term complications, including higher risk of heart disease and stroke. Aggressive treatment of hypertension and hyperlipideamia can result in a substantial reduction in cardiovascular events in patients with diabetes 1. Consequently pharmacist-led diabetes cardiovascular risk (DCVR) clinics have been established in both primary and secondary care sites in NHS Lothian during the past five years. An audit of the pharmaceutical care delivery at the clinics was conducted in order to evaluate practice and to standardize the pharmacists’ documentation of outcomes. Pharmaceutical care issues (PCI) and patient details were collected both prospectively and retrospectively from three DCVR clinics. The PCI`s were categorized according to a triangularised system consisting of multiple categories. These were ‘checks’, ‘changes’ (‘change in drug therapy process’ and ‘change in drug therapy’), ‘drug therapy problems’ and ‘quality assurance descriptors’ (‘timer perspective’ and ‘degree of change’). A verified medication assessment tool (MAT) for patients with chronic cardiovascular disease was applied to the patients from one of the clinics. The tool was used to quantify PCI`s and pharmacist actions that were centered on implementing or enforcing clinical guideline standards. A database was developed to be used as an assessment tool and to standardize the documentation of achievement of outcomes. Feedback on the audit of the pharmaceutical care delivery and the database was received from the DCVR clinic pharmacist at a focus group meeting.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Micro RNA genes and their likely influence in rice (Oryza sativa L.) dynamic ...Open Access Research Paper
Micro RNAs (miRNAs) are small non-coding RNAs molecules having approximately 18-25 nucleotides, they are present in both plants and animals genomes. MiRNAs have diverse spatial expression patterns and regulate various developmental metabolisms, stress responses and other physiological processes. The dynamic gene expression playing major roles in phenotypic differences in organisms are believed to be controlled by miRNAs. Mutations in regions of regulatory factors, such as miRNA genes or transcription factors (TF) necessitated by dynamic environmental factors or pathogen infections, have tremendous effects on structure and expression of genes. The resultant novel gene products presents potential explanations for constant evolving desirable traits that have long been bred using conventional means, biotechnology or genetic engineering. Rice grain quality, yield, disease tolerance, climate-resilience and palatability properties are not exceptional to miRN Asmutations effects. There are new insights courtesy of high-throughput sequencing and improved proteomic techniques that organisms’ complexity and adaptations are highly contributed by miRNAs containing regulatory networks. This article aims to expound on how rice miRNAs could be driving evolution of traits and highlight the latest miRNA research progress. Moreover, the review accentuates miRNAs grey areas to be addressed and gives recommendations for further studies.
Summary of the Climate and Energy Policy of Australia
Antigen.pptx
1. Antigen:
Antigen is a substance usually protein in nature and
sometimes polysachharide , and glycoproteins which when
introduced into vertebrate host can induce the specific
immune response either by producing specific antibodies or
especially sensitized T-cells or both.
Types of antigen:
1. Complete antigen or immunogen: Immunogenecity is the
inherent ability of a substance to induce the specific
immune response resulting in the formation of
antibodies.Immunogen are foreign substance which can
induce an immune response by themselves. Immunogen
are generally high molecular weight substances and
proteinous in nature.
2.
3. 2. Incomplete antigen or hapten: Hapten or incomplete
antigens are those which can't induce an immune
response by themselves. Haptens are not immunogenic
but are antigenic because they can react with immune
lymphocytes or antibodies.Haptens being incabable of
inducing immune response by themselves so they are
converted into immunogen with the specific molecule
known as carrier.Haptens are usually low molecular
weight substances and non-proteinous in nature.
Hapten-carrier conjugates have
native antigenic determinants of
the carrier as well as new
determinants of the hapten
4. All molecules that have the property of immunogenicity
also have the property of antigenicity but the reverse is not
true. For e.g some small molecules like haptens are
antigenic in nature but incapable by themselves of
inducing immune response.
Carrier molecule: The carrier molecules for hapten may be
serum proteins such as albumin,globulin or synthesized
polypeptides.Haptens are of two types:
•Complex haptens are relatively large molecules and
combine with specific antibody forming visible
precipitate.e.g Capsular polysacharides of Streptococcus
pneumoniae,Cardiolipin etc.
•Simple haptens do not form visible precipitate.
5. Factors influencing Immunogenicity:
The immune system actually recognize particular
macromolecules generally either proteins or polysaccharides.
Proteins are the most potent immunogens than
polysachharides.
Immunogenicity is infleunced by following 6 properties of
immunogen.They are:
•Chemical Composition:Majority of immunogens are protein
and glycoprotein in nature.They are the strongest antigens
because they have the largest array of potential building
blocks(amino acids).Heteropolymers(composed of different
amino acids)are usually more immunogenic than
homopolymers(composed of single amino acids).
6. •Molecular Size: Molecular size plays an important role in
immunogenicity of the molecule.Usually the larger
molecule is better immunogenic.Molecules having less
than 10,000 D mass cant induce immune response and are
called poor immunogens.e.g Drugs are poor immunogens.
•Genetic constituent of Host:The genetic constitution of an
immunized animal also influences the type of immune
response as well as the degree of response.For e.g
If polysachharide is injected, than no immune response is
induced by rabbit.
If polysachharide is injected than immune response is
induced by Guinea pig
7. •Foreignness:The immune system possess the
capacity to distinguish between self and non self
molecules. Antigen are non-self in nature because
our immune system generally produces no immune
response against self antigen. The greater the
phylogenetic distance between two species, the
greater the structural difference between them.For
.e.g The antigen Bovine serum albumin is not
immunogenic when injected into cow but strongly
immunogenic when injected into rabbit.
8. •Method/Route of administration and Dose:Dose
and route of administration of antigen is also a
factor of immunogenicity.Antigen administrated
intravenously is carried first to spleen where as
antigen administrated subcutaneously movest first
to local lymph nodes.Differences in the lymphoid
cells may be reflected in subsquent immune
response.An insufficient dose will not stimulate an
immune response and excessive dose also cant
induce immune response but are tolerable.For e.g
An 0.5 mg dose of antigen fails to induce an
immune response in mice,whereas a thousand fold
lower dose of same antigen can induce immune
response in Guinea pig.
9. •Antigen processing and presentation:The
development of both humoral and cell mediated
immune response requires interaction of T-cells with
antigen that has been processed and presented
together with MHC molecules. Larger, insoluble
macromolecules generally are more immunogenic
than small and soluble ones. Macromolecules or
Antigens that cannot be degraded and presented
with MHC molecules are poor
immunogen.Molecules which are polymer of L-
amino acids can easily degraded due to the presence
of degradative enzymes than D-amino acids.So our
body can recognize L-amino acids and acts as high
immunogens.
10. Antigen determinants(epitopes)
Immune cells donot interact with or recognise an entire
immunogen molecule,instead lymphocytes recognize
discrete sites on the macromolecules called epitopes or
antigenic determinants.
Epitopes are the sites on
or within the antigen with
Which antibodies react.
Immunogens or
macromolecules may
contain 1 or more epitope
but all epitope are not immunogenic.Epitopes are very
small i.e just four or five amino acids or monosachharides
residue 3 D structure and steric structure.
11. Epitopes determine the specificity of the antigen
molecule and induce the antibody response.
Antibodies are specific for epitopes.
There are two types of epitopes. They are:
1.B cell epitope
2.T cell epitope
B cell epitope: Antigenic determinants recognized
and bound by the B-cell receptor is the B cell
epitope.. Epitopes recognized by the B-cell
receptor are located on the surface of the antigen.
B cells recognize soluble antigen that is free in
solution,so B cell epitope should match paratope
of Antibody.
12.
13. T cell epitope:T cell receptors never bind free
antigens or epitopes. It can bind epitope that are
bound to MHC molecule. Epitopes recognized by the
T-cell receptor are often located in the inner,
unexposed side of the antigen, and become
accessible to the T-cell receptors after proteolytic
processing of the antigen.
T cell epitopes must be processed inside the cell so
as to bind to the MHC molecules. Hence T cell
bound to that epitope which are presented by antigen
presenting cells(APCs).
14.
15. Origin of Antigen:
Antigens can be classified in order of their origins.
•Exogenous antigen: Exogenous antigens are those which are
entered into the body from outside e.g by inhalation,ingestion or
injection. By endocytosis or phagocytosis, exogenous antigens are
taken into the antigen-presenting cells (APCs) and processed into
fragments. APCs then present the fragments to T helper cells (CD4+)
by the use of class II MHC molecules on their surface.
•Endogenous antigen: Endogenous antigens are generated within
our cells as a result of normal cell metabolism or because of viral or
intracellular bacterial infection. The fragments are then presented on
the cell surface in the complex with MHC class I molecules. If
activated cytotoxic CD8+ T cells recognize them, the T cells begin to
secrete various toxins that cause the lysis or apoptosisof the infected
cell.
16. •Auto antigen: An autoantigen is antigen which is protein
and are recognized as non self antigen by the immune
system of patients suffering from specific auto immune
diseases.The diseases condition where the immune system
attacks its own body parts is called auto immune system.
17. Sources of antigen:
•Particulate:
Cells e.g tumor cells, RBC, Leukocytes
Microorganisms e.g bacteria,viruses.
•Soluble antigens
Proteins
Polysachharides
Nucleic acids
Exotoxins and Super antigens.
18. Types of Antigen:
1. T-dependent antigens:T dependent antigens are those
which produces antibodies with the help of T-cells. In T-
dependent antigen T-cells produces cytokines which
gives the message to the B-cell for the production of
antibody.The produced antibody is monoclonal in
nature.
2. T- independent antigen:T-independent antigens are those
which can directly stimulate the B-cells to produce
antibody without the requirement of T-cell help.e.g
Lipopolysachharides.Due to polymeric structure of
LPS,B cell don’t require help of T-cell for the
production of Antibody. The produced antibody is
polyclonal in nature.
19.
20. 3. Super antigen: Super antigens activate a large number of
T-cells which harms the body.In some cases exposure to
super antigens can be fatal.e.g Styphylococcal toxic
shock toxin,Streptococcal pyrogenic exotoxin
etc.Normally when ag is encountered in body only 0.5%
T cells are activated but when super antigens are
encountered 25% T cells are activated.
Symptoms when super antigen is encountered are:
• High T cell activation
• Production of massive cytokines.
• Systemic effect like vasculation leakage
• Hypotension,fever,chill and shock and death.
Superantigens bind non specifically to the ß sub unit of
T-cell receptor and at the lateral site of MHC II
molecule.
21. 4. Cross-reactivity antigen (Heterophile antigen):
Certain antigens of similar nature present in
different tissues of more than one species are called
heterophile antigens or Substances that stimulate the
production of antibodies capable of reacting with
tissue of a wide variety of un related animals or
plants are called cross reacting antigen.
• Forssman antigen:
• Rickettsial antigen(Weil-felix reaction)
• Epstein Barr virus:
• Cardiolipin antigen:
22. • T cells recognize linear peptides not whole proteins.
• T cells only recognize epitopes when they are complexed
to MHC molecules.
• Therefore protein antigens must be processed and
presented by other cells to be recognized by T cells.
• This process is referred to as antigen processing and
presentation.
– Note: the association of antigenic peptides /MHC is a
saturable, low-affinity interaction with a slow off/on
rate.
• In general CD8+ T cells recognize endogenous antigens
and CD4+ T cells recognize exogenous antigens.
• MHC molecules bind multiple peptides, however each T
cell only recognizes one peptide.
Antigen Recognition by T Cells