Altering the specificity of T cell receptor (TCR) is one of the popular strategies to genetically modify T cells to enhance the tumor-killing activity of T cells. From a tumor-reactive T cell or active anti-tumor T-cell antigens, the appropriate target sequence is introduced to modify T cells to target a broad range of tumors with improved specificity. https://www.creative-biolabs.com/car-t/cellrapeutics-tcr-technology.htm

1. CellRapeutics™ TCR Technology
Reference: https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm

2. What is TCR?
• The T-cell receptor, or TCR, is a molecule found on
the surface of T cells, or T lymphocytes,[1] that is
responsible for recognizing fragments of antigen as
peptides bound to major histocompatibility complex
(MHC) molecules. The binding between TCR and
antigen peptides is of relatively low affinity and is
degenerate: that is, many TCRs recognize the same
antigen peptide and many antigen peptides are
recognized by the same TCR.

3. Introduction
• Altering the specificity of T cell receptor (TCR) is one of the popular strategies to genetically modify
T cells to enhance the tumor-killing activity of T cells. From a tumor-reactive T cell or active anti-
tumor T-cell antigens, the appropriate target sequence is introduced to modify T cells to target a
broad range of tumors with improved specificity. With engineering TCR construction and a series of
assay, such as transgene expression, TCR/CD3 stability, lymphocyte antigen reactivity and
cytotoxicity, animal experiments, etc., we provide cGMP-compliant TCR products for preclinical and
clinical trials.

4. Structure and function of the TCR

5. TCR Structure
• The T cell receptor (TCR), found on the surface of T cells, is responsible for antigen recognition. It
consists of two chains: the alpha (α) and beta (β) chains. Both chains have a constant region (c)
and a variable region (v), and it is the variable region that determines antigen specificity. The TCR is
associated with the CD3 complex, which comprises three transmembrane signalling molecules
(CD3ζζ, CD3δε and CD3γε).

6. TCR Structure
• A TCR will interact with an antigen on a target cell when the target peptide sequence is presented
by the appropriate major histocompatibility complex (MHC-1 for cytotoxic T cells). Efficient T-cell
activation also requires the simultaneous binding of the T cell co-receptor (CD8 for cytotoxic T cells).
ss, disulphide bridge.

7. Thanks
Reference: https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm