microscopic study of
diseased tissue after
use of special
preparation of the
For us every specimen is a patient, which we
have to make talk and tell about itself.
Routine (H&E) staining
Corner stone of tissue-based diagnosis.
Haematoxylin dye stains cell nuclei blue.
Eosin dye stains other structures pink or red.
This technique provides exceptional detail of
tissue structure and the makeup of the cells.
Special stains use a variety of dyes and
techniques to stain particular tissues,
structures or pathogens to assist pathologists
with tissue-based diagnosis.
MORPHOLOGIC DD OF HOMOGENOUS ACELLUAR
STAIN HYALINOSIS SCLEROSIS AMYLOID FIBROSIS FIBRIN
H&E +++ +++ ++ ++ +++
PAS +++ +++ + ++ +
MS - +++ - + -
RED/BLUE BLUE BLUE BLUE DARK
- - +++ - -
PAS (Periodic Acid-Schiff)
Stains basement membrane (normal and in
tumors), glycogen, some mucins and
Kidney: recommended for routine evaluation
of renal biopsies due to basement membrane
staining; also useful to diagnose renal cell
PAS STAINING OF A NORMAL GLOMERULOUS.
PAS highlights basement
membranes of glomerular
capillaries and tubular
Special stain for detecting fungi.
Stains Basement membranes.
There are several silver stains, including:
1) Grocott's methenamine silver stain, used
widely as a screen for fungal organisms.
2) Jones' stain, a methenamine silver-Periodic
acid-Schiff that stains for basement
SILVER STAINING IN MEMBRANOUS GN:
Highlights the membrane in black.The spikes of basement membrane are
Black material completely surrounds the immune
deposits forming rings.
SILVER STAINING IN MPGN:
Double contour or the “tram tracking” of the membranes because
Trichrome is a three colour staining protocol
used in histology.
The following staining is achieved:
1) Nuclei - blue/black.
2) Muscle, erythrocytes, cytoplasm – red
3) Connective tissue, in particular collagen -
TRICHROME STAINING IN FSGS:
TRICHROME STAINING IN MEMBRANOUS
The immune deposits with a
characteristic red color.
In stage 1,
In stage II,
In stage III,