Immunoassays such as ELISA and RIA are biochemical techniques that use the specificity of antigen-antibody binding to detect or quantify substances like proteins, hormones, and drugs. ELISA is a popular plate-based immunoassay that can be quantitative or qualitative. There are different types of ELISA including direct, indirect, sandwich, and competitive formats. RIA uses radioactive labeling for higher sensitivity to detect substances at the picogram level. Both techniques have applications in clinical diagnostics, pharmaceutical analysis, and research.
spectrofluorometer is the instrument for recording fluorescence emission and absorption spectra When a beam of light is incident on certain substances they emit visible light or radiations. This is known as fluorescence. Fluorescence starts immediately after the absorption of light and stops as soon as the incident light is cut off. The substances showing this phenomenon are known as flourescent substances.
spectrofluorometer is the instrument for recording fluorescence emission and absorption spectra When a beam of light is incident on certain substances they emit visible light or radiations. This is known as fluorescence. Fluorescence starts immediately after the absorption of light and stops as soon as the incident light is cut off. The substances showing this phenomenon are known as flourescent substances.
In this slide contains types, working principle, factors affecting, advantage and disadvantage of paper electrophoresis.
Presented by: G.Sai Swetha. (Department of pharmacology),
RIPER, anantapur.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
Immunofluorescence : Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens..
Fluorescein is a dye which emits greenish fluorescence under UV light. It can be tagged to immunoglobulin molecules.
This technique is sometimes used to make viral plaques more readily visible to the human eye.
Immunofluorescent labeled tissue sections are studied using a fluorescence microscope.
In this slide contains types, working principle, factors affecting, advantage and disadvantage of paper electrophoresis.
Presented by: G.Sai Swetha. (Department of pharmacology),
RIPER, anantapur.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
Immunofluorescence : Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens..
Fluorescein is a dye which emits greenish fluorescence under UV light. It can be tagged to immunoglobulin molecules.
This technique is sometimes used to make viral plaques more readily visible to the human eye.
Immunofluorescent labeled tissue sections are studied using a fluorescence microscope.
The following presentation contains helpful information regarding Radioimmunoassay (RIA) and Enzyme-Linked Immunosorbent Assay (ELISA), including their history, introduction, advantages, procedures and applications.
ELISA or Enzyme-linked Immunosorbent Assay is a qualitative and quantitative assay for detecting the presence of antigens (virus, hormones, enzymes, etc.) in a sample.
A RIA is a very sensitive in vitro assay technique used to measure concentrations of substances, usually measuring antigen concentrations (for example, hormone levels in blood) by use of antibodies.
Similar to immunological assays - ELISA and RIA (20)
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
2. IMMUNOASSAY
• Immunoassay means a method to measure any particular
substance in a mixture using its specific-binding antibody.
• One of the merits of immunoassay is that we can measure a
substance that is present in a mixture of various contaminants.
• Immunoassays have become very popular in view of their high
sensitivity, safety, economy and simple instrument
requirements.
2
3. ELISA
• ELISA is a biochemical technique used mainly in
immunology to detect the presence of an antibody or an
antigen in a sample.
• In this method the antigen or antibody is conjugated to an
enzyme.
• It is a plate based assays designed for detecting and
quantifying substances such as peptides, proteins, antibodies,
antigens and hormones.
• It involves detection of ‘analyte’ in a liquid sample using
liquid reagent (wet lab) or dry strips (dry lab).
• The test can be done in polystyrene tubes (macro-ELISA) or
polyvinyl microtitre plates (micro-ELISA).
3
5. BASIC PRINCIPLES
Based on Basic Immunology Response
•Lock and Key Concept
–Antigen (key): substance when introduced into the body
produces antibodies
–Antibody (lock): protein in the body that is used by immune
system to identify and neutralize foreign targets (referred to as
antigens)
–Key fits into the lock
•Enzyme conjugate substrates
–Enzyme that converts colourless substrates to a colored product
–Bound to the antibody that is part of the antibody-antigen
complex OR
–Bound to a secondary antibody that binds with the antibody-
antigen complex
5
6. ADVANTAGES OF ELISA TEST
• Sensitive: nanogram levels or lower
• Reproducible
• Minimal reagents
• Qualitative & Quantitative
Qualitative – Eg: HIV testing
Quantitative assays - Eg: TDM
• Greater scope : Wells can be coated with Antigens OR
Antibodies
6
7. • NO radiation hazards
• Fast—90 samples tested in 2-3 hr
• Sensitivity (up to 10 pg/mL)
• Specificity (sample with high concentration contaminants)
7
8. • Four common ELISA tests –based on the binding structure
between the Antibody and Antigen.
1.Direct-ELISA
2.Indirect-ELISA
3.Competitive-ELISA
4. Non-competitive-ELISA(Sandwich-ELISA)
8
9. Direct ELISA
• It uses the directly labelling the antibody itself.
• Microwell plates are coated with a sample containing the
target antigen, and the binding of labelled antibody is
quantitated by a colourimetric, chemiluminescent, of
fluorescent end-point.
1. Apply a sample of known antigen to a surface, often in the
well of a microtitre plate. The antigen is fixed to the surface
to render it immobile.
2. The plate wells or other surface are then coated with blocking
buffer.
3. Detecting antibody(labelled by an enzyme), usually diluted in
blocking buffer, is applied to the plate for binding to the
antigen coated on the plate.
9
11. • The plate is washed, so that unbound antibody is removed.
After this wash, only the antibody-antigen complexes remain
attached to the well.
• Apply a substrate which is converted by the enzyme to elicit a
chromogenic or fluorescent signal.
• View/quantify the result using a spectrophotometer or other
optical device.
• Positive and negative controls should always be included in
the test
• At every step, incubation and washing is done to wash off
unbound reagents.
11
12. Advantages of Direct ELISA:
• Quick methodology since only one antibody is used.
• Cross-reactivity of secondary antibody is eliminated.
Disadvantage of Direct ELISA:
• Immuno-reactivity of the primary antibody may be reduced as
a result of labelling.
• Labelling of energy primary antibody label from one
experiment to another.
• Little signal amplification.
12
13. Indirect ELISA
• For antibody detection, the wells of microtitre plates are
coated with antigen.
• Sera to be tested are added in these coated wells.
• If antibody is present,antigen-antibody reaction takes place.
• To detect this reaction, a goat antihuman immunoglobulin
antibody conjugated with an enzyme is added.
• A substrate is added and enzyme acts on substrate to produce a
colour .
13
15. Advantages of Indirect ELISA :
• High sensitivity: More than one labelled antibody is bound
per antigen molecule;
• Flexible: Different primary detection antibodies can be used
with a single labelled secondary antibody;
• Cost-saving: Fewer labelled antibodies are required.
15
17. • A Sandwich ELISA
1. Plate is coated with a capture antibody;
2. sample is added, and any antigen present binds to capture
antibody;
3. detecting antibody is added, and binds to antigen;
4. enzyme-linked secondary antibody is added, and binds to
detecting antibody;
5. substrate is added, and is converted by enzyme to
detectable form.
6. The positive results produces color which can be read by
ELISA reader. 17
18. Sandwich ELISA advantages:
• High specificity, since two antibodies are used the
antigen/analyte is specifically captured and detected.
• Suitable for complex samples, since the antigen does not
require purification prior to measurement.
• Flexibility and sensitivity, since both direct and indirect
detection methods can be used.
18
19. COMPETITIVE ELISA TEST
Here competition occurs between two antibodies for the same
antigen.
It has been used for detection of HIV antibodies.
The microtiter plate wells are coated with HIV antigen.
Sera to be tested is added to these wells and incubated at
37degree C and washed
Antigen-Antibody reaction occurs
19
21. To detect this reaction ,enzyme labelled specific HIV
antibodies are added
These antibodies remain free because there is no antigen left
to react
Substrate is added but there is no enzyme to act .Hence
positive results show no color.
If serum to be tested is negative for antibodies, antigen is
there to combine with enzyme conjugated antibodies and
reacts with substrate to produce colour.
21
22. Competitive ELISA advantages:
• High specificity, since two antibodies are used the
antigen/analyte is specifically captured and detected
• Suitable for complex samples, since the antigen does not
require purification prior to measurement
• Flexibility and sensitivity, since both direct and indirect
detection methods can be used
22
23. APPLICATIONS
• Detection of HIV antibodies in serum.
• Detection of mycobacterial antibodies in tuberculosis.
• Detection of rotavirus in faeces.
• Detection of hepatitis B markers in serum.
• Detection of enterotoxin of E coli in faeces.
23
25. RADIOIMMUNOASSAY
Introduction:
• The technique was introduced in 1960 by Berson and Yalow
as an assay for the concentration of insulin in plasma.
• It represented the first time that hormone levels in the blood
could be detected by an in vitro assay.
• The sensitivity range is 0.0006–0.006 μg antibody/ml
25
26. RIA
RADIOIMMUNOASSAY(RIA) is a very sensitive invitro assay
technique in which antibody or antigen is labelled with a
radioactive material(125I).
• It is used to measure concentrations of antigens using
specificity of antigen-antibody binding and quantization using
radioactivity.
• It is also called as binder-ligand assay where binder is the
component to which radioactive material is labelled and
ligand(antigen or antibody) is the component which is to be
detected.
• It is more specific and sensitive method and can detect antigen
upto picogram quantities.
26
27. PRINCIPLE OF RIA
It involves three principles:
• An immune reaction i.e. antigen, antibody binding.
• A competitive binding or competitive displacement reaction.
(It gives specificity)
• Measurement of radio emission. (It gives sensitivity)
27
29. PROCEDURE
A known quantity of an antigen is made radioactive, frequently
by labelling it with γ-radioactive isotopes of iodine attached to
tyrosine.
This radio labelled antigen is then mixed with a known amount
of antibody for that antigen.
A sample of serum from a patient containing an unknown
quantity of same antigen is added.
This causes the unlabelled(cold) antigen from serum to
compete with radio labelled antigen(hot) for antibody binding
site. 29
30. As the concentration of cold antigen is increased , more of it
binds to antibody, displacing the radio labelled variant and
reducing the ratio of antibody bound radio labelled antigen to
free radio labelled antigen.
The bound antigens are then separated from the unbound ones,
and the radioactivity of the free antigen remaining in the
supernatant is measured using a γ counter.
The concentration of the test antigen can be calculated from
the ratio of the bound and total antigen labels , using a
standard dose response curve.
30
31. From these data, a standard binding curve, like the one shown
in red, can be drawn.
The samples to be assayed (the unknowns) are run in parallel.
After determining the ratio of bound to free antigen in each
unknown, the antigen concentrations can be read directly from
the standard curve.
31
32. Advantages & disadvantages
Advantages
• Highly specific: Immune reactions are specific
• High sensitivity : Immune reactions are sensitive
Disadvantages
• Radiation hazards: Uses radiolabelled reagents
• Requires specially trained persons
• Labs require special license to handle radioactive material
• Requires special arrangements for requisition, storage of
radioactive material ,radioactive waste disposal.
32
33. APPLICATIONS
• Detection of narcotic drugs
• Blood bank screening for the hepatitis (a highly contagious
condition) virus.
• Measurement of growth hormone levels, immunoglobulin's,
tumour markers
• Tracking of the leukaemia virus
• Diagnosis and treatment of peptic ulcers and
33
34. • Endocrinology
– Insulin, HCG, Vasopressin
– Detects Endocrine Disorders
– Physiology of Endocrine Function
• Pharmacology
– Morphine
– Detect Drug Abuse or Drug Poisoning
• Study Drug Kinetics.
34
35. • Clinical Immunology
– Antibodies for Inhalant Allergens
– Allergy Diagnosis
• Oncology
– Carcino-embryonic Antigen
– Early Cancer Detection and Diagnosis
35
36. References
Gupte S. The short textbook of medical microbiology. 8th ed.,
150-52.
Michael J P, Pelczar E C S. Microbiology. 5th ed., 734-36
Chakraborthy P. Textbook of microbiology. 2nd ed., NCBA
Ltd, 190-95
Baveja C P. Textbook of Microbiology. 2nd ed., 116-20
Kokare C R. Pharmaceutical Microbiology. 2nd ed., Nirali
Prakashan, 23.14.
Ashutoshkar. Pharmaceutical Drug Analysis. 2nd ed., 485-90.
36