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Riboflavin (Vitamin B2)
• Riboflavin which has also known as Vitamin B2, Vitamin G and “
the yellow enzyme” was recognized as a vitamin in 1917.
• As early as 1897, a London chemist had observed in milk whey a
water-soluble pigment with peculiar yellow-green fluorescence.
• It was given the chemical group name flavin from the Latin word
for Yellow.
• Later because the vitamin was found also to contain a sugar
named ribose, the name riboflavin was adopted.
Riboflavin (Vitamin B2) - Chemistry
• Riboflavin through its coenzymes takes part in
a variety of cellular oxidation-reduction
reactions.
• Riboflavin contains 6,7-dimethyl
isoalloxazine ( a hetrocyclic 3 ring structure)
attached to D-ribitol by a nitrogen atom.
Riboflavin (Vitamin B2) - Chemistry
Riboflavin (Vitamin B2) - Chemistry
• Coenzymes of Riboflavin
• Flavin mononucleotide (FMN) and Flavin
Adenine dinucleotide (FAD) are the two
coenzyme forms of riboflavin. The ribitol (5
carbon) is linked to a phosphate in FMN. FAD
is formed from FMN by the transfer of an AMP
moiety from ATP.
Riboflavin (Vitamin B2) - Biochemical
functions
I. The flavin coenzymes (mostly FAD and to a lesser extent
FMN) participate in many redox reactions responsible for
energy production.
• The functional unit of both the coenzymes is isoalloxazine
ring which serves as an acceptor of two hydrogen atoms
(with electrons).
• FMN or FAD undergo identical reversible reactions
accepting two hydrogen atoms forming FMNH2 or
FADH2.
Riboflavin (Vitamin B2) – Biochemical
Functions
II. Enzymes that use flavin coenzymes (FMN or FAD) are
called flavoproteins.
• Many flavoproteins contain metal atoms (iron, molybdenum
etc.) which are known as metalloflavoproteins.
• The coenzymes, FAD and FMN are associated with several
enzymes involved in carbohydrate, lipid, protein, and purine
metabolisms, besides the electron transport chain.
Riboflavin (Vitamin B2) - Recommended
dietary allowance (RDA)
• The daily requirement of riboflavin for an
adult is 1.2-1.7mg.
• Higher intakes (by 0.2-0.5 mg/day) are
advised for pregnant and lactating women.
Riboflavin (Vitamin B2) – Dietary
Sources
• Milk and milk products, meat, eggs, liver,
kidney, are rich sources.
• Cereals, fruits, Vegetables and fish are
moderate sources.
Riboflavin (Vitamin B2) - Deficiency
Symptoms
• Riboflavin deficiency symptoms include:
• Cheilosis (fissure at the corner of the mouth),
• Glossitis (tongue smooth and purplish) and
• Dermatitis.
• Assay of the enzyme Glutathione Reductase in
erythrocytes will be useful in assessing the
riboflavin deficiency.
Vitamin b2
Vitamin b2

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Vitamin b2

  • 1.
  • 2.
  • 3. Riboflavin (Vitamin B2) • Riboflavin which has also known as Vitamin B2, Vitamin G and “ the yellow enzyme” was recognized as a vitamin in 1917. • As early as 1897, a London chemist had observed in milk whey a water-soluble pigment with peculiar yellow-green fluorescence. • It was given the chemical group name flavin from the Latin word for Yellow. • Later because the vitamin was found also to contain a sugar named ribose, the name riboflavin was adopted.
  • 4.
  • 5. Riboflavin (Vitamin B2) - Chemistry • Riboflavin through its coenzymes takes part in a variety of cellular oxidation-reduction reactions. • Riboflavin contains 6,7-dimethyl isoalloxazine ( a hetrocyclic 3 ring structure) attached to D-ribitol by a nitrogen atom.
  • 6.
  • 8.
  • 9. Riboflavin (Vitamin B2) - Chemistry • Coenzymes of Riboflavin • Flavin mononucleotide (FMN) and Flavin Adenine dinucleotide (FAD) are the two coenzyme forms of riboflavin. The ribitol (5 carbon) is linked to a phosphate in FMN. FAD is formed from FMN by the transfer of an AMP moiety from ATP.
  • 10.
  • 11.
  • 12. Riboflavin (Vitamin B2) - Biochemical functions I. The flavin coenzymes (mostly FAD and to a lesser extent FMN) participate in many redox reactions responsible for energy production. • The functional unit of both the coenzymes is isoalloxazine ring which serves as an acceptor of two hydrogen atoms (with electrons). • FMN or FAD undergo identical reversible reactions accepting two hydrogen atoms forming FMNH2 or FADH2.
  • 13. Riboflavin (Vitamin B2) – Biochemical Functions II. Enzymes that use flavin coenzymes (FMN or FAD) are called flavoproteins. • Many flavoproteins contain metal atoms (iron, molybdenum etc.) which are known as metalloflavoproteins. • The coenzymes, FAD and FMN are associated with several enzymes involved in carbohydrate, lipid, protein, and purine metabolisms, besides the electron transport chain.
  • 14.
  • 15.
  • 16. Riboflavin (Vitamin B2) - Recommended dietary allowance (RDA) • The daily requirement of riboflavin for an adult is 1.2-1.7mg. • Higher intakes (by 0.2-0.5 mg/day) are advised for pregnant and lactating women.
  • 17.
  • 18. Riboflavin (Vitamin B2) – Dietary Sources • Milk and milk products, meat, eggs, liver, kidney, are rich sources. • Cereals, fruits, Vegetables and fish are moderate sources.
  • 19.
  • 20. Riboflavin (Vitamin B2) - Deficiency Symptoms • Riboflavin deficiency symptoms include: • Cheilosis (fissure at the corner of the mouth), • Glossitis (tongue smooth and purplish) and • Dermatitis. • Assay of the enzyme Glutathione Reductase in erythrocytes will be useful in assessing the riboflavin deficiency.