This document discusses the use of flow cytometry immunophenotyping for leukemia diagnosis at Moi Teaching and Referral Hospital. It provides background on the WHO classification of hematopoietic tumors and describes the process of specimen collection, staining with monoclonal antibodies, and analysis using flow cytometry. The study found that flow cytometry results agreed with morphological diagnosis in 66.7% of ALL cases and 77.8% of AML cases, with discrepancies sometimes due to aberrant marker expression or few events analyzed. Flow cytometry is an important tool for leukemia diagnosis but must be used along with morphology and clinical information.
Delayed blood transfusion reaction is a reaction too blood transfusion occurring after 24 hours. Can be divided to immune mediated and non-immune mediated. Share about the cause, symptoms, investigations and management.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
Delayed blood transfusion reaction is a reaction too blood transfusion occurring after 24 hours. Can be divided to immune mediated and non-immune mediated. Share about the cause, symptoms, investigations and management.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
UAEU - CMHS - Hematology-Oncology Course - MMH 302 - HONC 320. Education material for medical students - It cover basic principles of hematology and oncology, including CAR-T and gene editing. It can be used for study and review. It illustrates main principles of hematology and oncology.
Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
Basic approach to a case of anemia. Investigations to do and to arrive at the diagnosis. (Management not discussed). Peripheral smear findings with pictures are included.
This slide show forms part of the Introduction to Flow Cytometry seminar help by The Garvan MLC Flow Cytometry Facility. The Garvan MLC Flow Cytometry Facility is part of the Garvan Institute of Medical Research and is located in Sydney NSW.
UAEU - CMHS - Hematology-Oncology Course - MMH 302 - HONC 320. Education material for medical students - It cover basic principles of hematology and oncology, including CAR-T and gene editing. It can be used for study and review. It illustrates main principles of hematology and oncology.
Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
Basic approach to a case of anemia. Investigations to do and to arrive at the diagnosis. (Management not discussed). Peripheral smear findings with pictures are included.
This slide show forms part of the Introduction to Flow Cytometry seminar help by The Garvan MLC Flow Cytometry Facility. The Garvan MLC Flow Cytometry Facility is part of the Garvan Institute of Medical Research and is located in Sydney NSW.
A presentation on chemotherapy, for CHSS SND 2D Class with Ms. Weston. Includes awesomeness from the creators, and spunkiness from the assigner. To be viewed and enjoyed by everyone.
:)
Have a good one!
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research...iosrphr_editor
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research paper publishing, where to publish research paper, journal publishing, how to publish research paper, Call for research paper, international journal, publishing a paper, call for paper 2012, journal of pharmacy, how to get a research paper published, publishing a paper, publishing of journal, research and review articles, Pharmacy journal, International Journal of Pharmacy, hard copy of journal, hard copy of certificates, online Submission, where to publish research paper, journal publishing, international journal, publishing a paper
Study of some Pulmonary Function Tests in Children with Sickle Cell Anemia: C...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Clinical diagnosis of chronic myeloid leukemia by real time polymerase chain ...Teboho Mooko
Oncology study i did in my third year (2014). the study was basically about monitoring Chronic Myeloid leukemia (CML) using Real-Time PCR techniques to check how patients from Universitas Hospital responded to the treatment of Gleevec drug.
Factory Supply Best Quality Pmk Oil CAS 28578–16–7 PMK Powder in Stockrebeccabio
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Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Use of flow cytometric immunophenotyping by teresa lotodo
1. USE OF FLOW CYTOMETRY IMMUNOPHENOTYPING FOR
DIAGNOSIS OF LEUKEMIA AT MOI TEACHING AND REFERRAL
HOSPITAL
Dr. Lotodo T.L.C , MBchB (MoiUni), Mmed
Path (UoN)
2. Back ground
World Health Organization classification of
tumours of haematopoietic and lymphoid
tissues is a collaborative project of the European
Association of Haematopathology and the Society
for Haematopathology.
The project began in 1995 and brought together
over 50 pathologists and oncologists to revise
the classification of hemopoeitic malignancies
based on the REAL
The 2008 revision of the World Health Organization (WHO)
classification of myeloid neoplasms and acute leukemia: rationale
and important changes, Blood. 2009;114(5):937.
3. Backg….
REAL classification of lymphoid was based upon
clinical features, morphology, immunophenotype,
and genetics
In 2001 3rd edition was made and in 2008 4th
edition was made.
WHO classification broadly classifies the
,malignancies as
myeloid,lymphoid,histiocytic/dendritic and mast
cell disorders
4. Introduction
Flow cytometry is a technology that
simultaneously measures and then analyzes
multiple physical characteristics of single
particles, usually cells, as they flow in a fluid
stream through a beam of light.
The properties measured include a particle’s
relative size, relative granularity or internal
complexity, and relative fluorescence intensity.
These characteristics are determined using an
optical-to-electronic coupling system
5. Specimen processing
Specimens commonly analyzed are bone
marrow aspirate, peripheral blood and
lymphoid tissues FNA material and body
cavity fluids
Blood and BMA is processed within 24-48hrs of
collection stored at room temp
They are collected into Heparin or EDTA tube
Tissues like lymphnodes and trephines, trephine
are submitted in culture media e.g RPMI to
maintain viability
The tissues are mechanically dissociated with a
scalpel to yield a cell suspension
6. Spec…
A cell count is obtained before flow is run-
100,000 events or more required.
Red cells are lysed to obtain a population of
nucleated cells before staining
The sample is then stained with a cocktail of
fluorochrome conjugated monoclonal
antibodies
Analysis of intracytoplasmic markers require an
additional fixation and permeabilization step to
allow antibodies to pass through the cell
membrane
A predetemined panel of antibodies may be used
7. Analysis
A measurement for the
diffraction of light in a
flat angle is the forward
scatter (FSC), which
depends on the volume
of the cell.
A measurement for the
diffraction of light in a
right angle is the so
called sidewards
scatter (SSC). It
depends on the
granularity
The process of
collecting data from
samples using the flow
cytometer is termed
'acquisition‘
9. Acute promyelocytic leukemia
CD34 and HLADR
are characteristically
negative in APL.
CD13 and CD117 are
usually
positive. CD33 and
CD13 are expressed
in APL.
11. Study
Objective: The main objective of the study was to
compare morphological and flow cytometric diagnosis
in patients previously diagnosed with leukemia
Materials and Methods: Retrospective study was
carried out at Moi Teaching and Referral Hospital
between the period July 2013 and June 2014 After
Institutional Research Ethics approval was granted
Consecutive sampling was done and information was
extracted from patients files that were previously
diagnosed with leukemia
The data was collected using a data collection form
where socio-demographic data, morphological and
flow cytometry results were recorded.
12. Morphological diagnosis
The original classification scheme proposed by
the French-American-British (FAB) Cooperative
Group divides AML into 8 subtypes (M0 to M8)
ALL into 3 subtypes (L1 to L3)
13. Flow cytometry
Equipment: Four colour computerized BD FACS.
The fluorochromes FITC, PE, Per CP, and APC
were applied
Panel of monoclonal antibodies used include:
-Pan Leukocyte antigen: CD 45 is a pan leukocyte
marker
- B- cell– CD10, CD19, CD20, cyt CD79a
-T cell; CD3, CD4, CD7, CD8, cyt CD3
-Myeloid; CD33, CD117, MPO.
-Immature cell antigens; CD34, and HLADR
-Erythroid marker: CD71
14. Results
The total number of samples run between July
2013 and June 2014 were 101, diagnosed as:
i)AML-11,
ii)ALL-32
iii)Negative-44
iv)Inclonclusive-14
The findings for this study were based on 33
results for patients who had both flow cytometry
and morphological diagnosis
15.
16.
17. Results
Morphology
+ve -ve Total
Flow +ve 12 6 18
-ve 3 11 14
Total 15 17 32
The probability of a
person having the ALL
(morphology)and
Cytometry test
showing positive
results is 66.7%
18. Results
Morphology
+ve -ve Total
Flow +ve 7 2 9
-ve 10 13 23
Total 17 15 32
The probability of a
person having the
AML(morphology) and
Cytometry test
showing positive
results is 77.8%
19. Discrepancies of flow and
morphology
Serial
no
Morphology Flow Comment
003 AML M5 CD45(dim)-95%,
CD33(95%), CD38(86%),
HLADR(92%),
CytCD3(95%), MPO-4% -
Reported as T-ALL
Biphenotypic
006 AML M5 CD 45(dim)-21%, cytCD7a-
21%-Reported as B-ALL
Few events-
70,000
007 Inconclusive CD45(dim)-66%,
CD38(51%),CD33(50%)
HLADR(43%)-Reported as
AML
Inadequate for
morphological
evaluation
016 AML M2 CD45(bright)-30%,
CD5(31%)
Few events-
15,000
018 ALL CD45dim)-73%, CD5(61%),
cyt CD3(34%),
MPO(41%),CD71(74%)-
Biphenotypic
20. Discrepancies of flow and
morphology
Serial
no
Morphology Flow Comment
019 AML M1 CD45(dim)-82%,
CD33(74%), HLADR(73%),
cytCD3(93%)-Reported as
T-ALL
Biphenotypic
024 ALL CD45 (bright)-28%,
CD3(20%)-Inconclusive
results
Few events-
10,000
025 AML M1 CD45(bright)-
54%,CD45(dim)-22%
CD19(24%), CD3(54%),
CD5(52%),HLADR-35%,
CD79a(23%)-Reported as
T-ALL
Few events-
10,000
21. WHO-2008:
MWWHOHixeWenotype Acute
LeukemiaWHOW
B cell lineage T cell lineage Myeloid lineage
Strong CD19
with :
cCD22, CD10 or
cCD79a
CD3 (c or s) cMPO
CD19 with at least
two of:
cCD22, CD10 or
cCD79a
2 or more of: CD11c,
CD14, CD36, CD64
Vardiman et al., Blood 2009, 114:937-51
22. Pit falls in morphology and flow
diagno.
Morphology
Differentiating ALL
and AML M0 /M1
Aspicular/hemodilute
BMA
Viral infections
involving marrow-large
abnormal cells
that look like blasts
Flow cytometry
Few events<100,000
Poor sample
collection
Abberant expression
of some markers
Few flow cytometrists
Consultation services
23. Conclusions
Flow cytometry is an important diagnostic tool for
diagnosis of acute leukemia and should be
available for patients in public hospitals.
It should be used together with adequate clinical
info and morphology for comprehesive diagnosis
Future-cytogenetics can be developed through
proposal writing for grants
24. Acknowledgments
Moi University /MTRH-Kirtika Patel (PI), Festus
Njuguna, Wilfred Emonyi, Simeon Mining, Nathan
Buziba, Nicholas Kigen
Indiana University-Magdeline Czader, Asirwa
Chite, Terry Vik, Jodi Skiles
V U University medical center, Natherlands-
Marissa Westers, Valerie de Haas, Floor Abink,
Gertjan Kaspars
Editor's Notes
By EGIL not
diagnosed as MPAL:
Those AL expressing only MPO
By WHO not diagnosed as MPAL
Those AL classified by EGIL without expressing MPO