This document discusses autoantibodies known as antinuclear antibodies (ANA) that are directed against nuclear antigens. It covers the prevalence of ANA, various nuclear antigens they may be directed against like DNA and histones, mechanisms of autoimmunity and autoantibody production, roles of B cells and T cells, and methods of detecting ANAs. It also describes different patterns of ANA fluorescence and their associations with various autoimmune diseases.
There are very few tests which are simple to carry out, done in almost all centers, use full to narrow down the list of provisional diagnosis and also useful for predicting future outcomes. C-RP is one of them. this presentation is not only for laboratory personal but also for MBBS students. Dental students, clinical department post graduates as well as clinicians.
There are very few tests which are simple to carry out, done in almost all centers, use full to narrow down the list of provisional diagnosis and also useful for predicting future outcomes. C-RP is one of them. this presentation is not only for laboratory personal but also for MBBS students. Dental students, clinical department post graduates as well as clinicians.
Internal quality control (IQC) in coagulation labAnkit Raiyani
In the haematology laboratory it is essential to ensure that the right test is carried out on the right specimen and that the correct results are delivered to the appropriate recipient without delay.
Quality control (QC) is defined as measures that must be included during each assay run to verify that the test is working properly.
Internal quality control (IQC) is monitoring the haematology test procedures to ensure continual evaluation of the reliability of the daily work of the laboratory with validation of tests before reports are released
Immunological Disorders can be classified into 3 distinct categories.They are Hypersensitivity, Autoimmunity and Immunodeficiency.Here in this presentation we talk about Immunodeficiency disorders.Get more on our blog : http://dentistryandmedicine.blogspot.com/
Autoimmune hemolytic anemia (or autoimmune haemolytic anaemia; AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to insufficient plasma concentration.
cytochemical stains. CML versus Leukamoid. LAP score. NAP score. Hematology, Hematopathology. Lab technology. Pahology. Medical Laboratory. White cell stains
Internal quality control (IQC) in coagulation labAnkit Raiyani
In the haematology laboratory it is essential to ensure that the right test is carried out on the right specimen and that the correct results are delivered to the appropriate recipient without delay.
Quality control (QC) is defined as measures that must be included during each assay run to verify that the test is working properly.
Internal quality control (IQC) is monitoring the haematology test procedures to ensure continual evaluation of the reliability of the daily work of the laboratory with validation of tests before reports are released
Immunological Disorders can be classified into 3 distinct categories.They are Hypersensitivity, Autoimmunity and Immunodeficiency.Here in this presentation we talk about Immunodeficiency disorders.Get more on our blog : http://dentistryandmedicine.blogspot.com/
Autoimmune hemolytic anemia (or autoimmune haemolytic anaemia; AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to insufficient plasma concentration.
cytochemical stains. CML versus Leukamoid. LAP score. NAP score. Hematology, Hematopathology. Lab technology. Pahology. Medical Laboratory. White cell stains
This is a journal club presentation where we present good quality papers from leading journals of the world. This particular paper deals with new biomarkers for Rheumatoid Arthritis.
A presentation detailing the symptoms, pathogenicity, factors affecting, diagnosis and treatment of the autoimmune disorder systemic lupus erythematosus
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
3. Anti nuclear antibodies
(auto)antibodies reactive with antigens in nucleoplasm
POSITIVE when titre above normal level
Most ANAs – directed against nucleic acid or
associated proteins
First demonstration- in 1957 by Holborow et al using
indirect immunofluoroscence
In SLE- predominant antigen is nucleosome
5. ANA
Physiological
Low titer
IgM
Secreted by CD5+B cells
Resides in peritoneum
Weak avidity for self Ag
Widely cross-reactive with
multiple Ag
Pathological
High titer
IgG
Secreted by CD19+B cells
Resides in hematopoietic
system
High avidity for self Ag
Have restricted specificity
7. Anti-DNA and nucleosome autoantibodies
recognize histone-DNA complexes
Autoantibodies against chromatin and nucleosomes in
SLE are diverse, recognizing the H2A-H2B-DNA
complex, as well as individual histones, ssDNA, and
dsDNA
About 70% of SLE patients have anti-dsDNA
antibodies at some time in their disease course
8. Anti-Sm and RNP autoantibodies recognize the
U1 ribonucleoprotein
proteins U1-70K, A, B’/B, C, D1/2/3, E, F, and G
associated with U1 sn RNA
B’/B, D, E, F, and G assemble into Sm core particle
Anti sm- SLE
Anti 70K, A, C – PM, Scleroderma, SLE
High levels of anti-RNP without anti-Sm - MCTD
9. Anti-Ro60 and La antibodies recognize cytoplasmic
ribonucleoproteins
Associated with sicca syndrome
Anti-Ro60:60-80% of primary Sjogren syndrome and
10-50% SLE patients
Anti Ro60 bind to 60-kDa protein associated with Y1,
Y3, Y4, or Y5 RNA.
47-kDa La antigen is a termination factor for RNA
polymerase III
10.
11. Development of autoimmunity
defects in the Fas-Fas ligand (FasL) pathway
defects in endosomal DNA degradation
defects in degradation of misfolded RNA
defects in the clearance of apoptotic cells by
phagocytes
over-expression of TLRs
MECHANISM OF SLE
12. Autoantigenesis
Mechanisms:
Defective apoptosis
Impaired removal of apoptotic cells
Mutation
Genetic polymorphisms
Alternate splicing
Post translational modification
Molecular mimicry
Defective sensing and uptake of autoAg
13. How does auto-Ag produce
auto-antibody
Induction of effector T cells
Reduced activation of suppressor cells
Activation of TLRs
Others-
Acting as chemoattractants
Altered recognition of auto-antigens
14. Role of T cells
T-cell-independent response-rapid antibody
production by short-lived plasma cells that
develop extrafollicularly and produce low-affinity
IgM antibodies
T-cell-dependent response– in secondary
lymphoid organs e.g. spleen or lymph nodes,
which provide an optimal milieu for interactions
between T cells, B cells, and APCs
17. IFN-1 rescues autoraective T cells from apoptosis
Newer therapy directed at co-stimulation:
CTLA4-Ig: potent inhibitor of co-stimulation and thus
T-cell activation
18. Role of B cells
B1- IgM-high, IgD-low, CD23-neg
T cell independent
produces low affinity Ab
B2 (conventional)-IgM-low,IgD-high, CD23-high,CD5- neg
requires CD 4+ T cells
high affinity Ab production
Deletion (apoptosis), anergy, receptor editing, and
immunological ignorance -determines whether B cells
become activated or are tolerized
19.
20. High-affinity B cells tend to develop into short-
lived plasma cells, whereas lower-affinity B cells
enter germinal centers and are more likely to become
long-lived plasma cells and memory cells.
Maintenance of long-lived plasma cells requires
continuous expression of Blimp-1 ,IL-6 and BAFF
produced by stromal cells
21. Extra follicular generation of
autoantibodies
anti-DNA and rheumatoid factors, can develop
extrafollicularly without T cells
T-cell independent
exhibit significant somatic hyper mutation and class
switching
22. Role of BAFF/APRIL
interact differentially with three receptors: BAFF-R ,TACI,
and BCMA.
BAFF/BAFF-R interactions regulate the differentiation and
survival of B-2 and MZ B cells
APRIL regulates CD40-independent class-switching and
plasma cell survival
TACI regulates TI-2 responses.
23. CD40-CD40 ligand signalling
CD40-CD40L interactions are important for generating
high-affinity autoantibodies.
Newer therapeutic options:
1. human monoclonal anti-BAFF antibody : BELIMUMAB
2.anti-CD40L reduces anti-dsDNA antibodies
24.
25. Basic mechanism of ANA production
Breakdown of tolerance
Transformation of autoreactive B cells
into long lived plasma cell & memory B
cells
26.
27. Sensitivity Specificity
IIF 80% 90-97%
ELISA 99% 36-94%
IIF- gold standard
ELISA
Immunodiffusion
CIEP
Multiple immunobead assay
Methods of detection of ANA