Modification n animal genome transgenic animal useful fr get some valuable therapeutics model animals
human being trans genesis is illegal but some don't respect
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
Refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation.
Foreign DNA is introduced into the animal, using recombinant DNA technology,
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
Refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation.
Foreign DNA is introduced into the animal, using recombinant DNA technology,
Introduction.
Definition.
Importance of transgenic animals.
Transgenic mice
Methods for introducing a foreign gene:
The retroviral vector method
The DNA microinjection method/ pronuclear microinjection
Genetically engineered embryonic stem cells
Transgenic fish
What is transgenic fish?
A few facts to know to know about transgenic fish.
Important points needed for genetic engineering (gene transfer) to produce transgenic fish.
Development of transgenic fishes.
A few examples
Auto-transgenesis.
Controlled culture of transgenic fish and feed.
Gene transfer technology for development of transgenic fishes.
Gene flow.
Food safety issues.
Conclusion.
Bibliography.
Transgenesis is the future of healthcare where the world is focusing on it so why not us? Let's delve into the exclusive depth of this transgenesis in the slide.
Transgenic animal models for the functional analysis of vasoactive peptidesWaliullah Wali
The renin–angiotensin system (RAS) is a hormone system that regulates blood pressure and fluid balance.
renin–angiotensin system (RAS) is developed in animal by transgenic technology and effects of vasoactive peptide are seen.
Vasoactive peptide is a peptide hormone containing 28 amino acid residues.
Vasoactive peptide is produced in many tissues including the gut, pancreas, and suprachiasmatic nuclei of the hypothalamus in the brain.
It stimulates contractility of heart, causes vasodilation, lowers arterial blood pressure.
Vasoactive peptide has a half-life (t½) in the blood of about two minutes.
Transgenic technology has established to be very useful for the functional analysis of vasoactive peptide systems.
A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.
Transgenesis is the process by which mixing up of genes takes place.
Foreign genes are inserted into the germ line of the animal, so it can be transmitted to the progeny.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.
First transgenic animal was a ‘Supermouse’ created by Ralph Brinster (U Pennsylvania) and Richard Palmiter (University of Washington) in 1982.
It was created by inserting a human growth hormone gene in mouse genome.
The offspring was much larger than the parents.
Mouse – common transgenic expt.
Other animals include pig, goat, cow, sheep, fish etc.
Introduction.
Definition.
Importance of transgenic animals.
Transgenic mice
Methods for introducing a foreign gene:
The retroviral vector method
The DNA microinjection method/ pronuclear microinjection
Genetically engineered embryonic stem cells
Transgenic fish
What is transgenic fish?
A few facts to know to know about transgenic fish.
Important points needed for genetic engineering (gene transfer) to produce transgenic fish.
Development of transgenic fishes.
A few examples
Auto-transgenesis.
Controlled culture of transgenic fish and feed.
Gene transfer technology for development of transgenic fishes.
Gene flow.
Food safety issues.
Conclusion.
Bibliography.
Transgenesis is the future of healthcare where the world is focusing on it so why not us? Let's delve into the exclusive depth of this transgenesis in the slide.
Transgenic animal models for the functional analysis of vasoactive peptidesWaliullah Wali
The renin–angiotensin system (RAS) is a hormone system that regulates blood pressure and fluid balance.
renin–angiotensin system (RAS) is developed in animal by transgenic technology and effects of vasoactive peptide are seen.
Vasoactive peptide is a peptide hormone containing 28 amino acid residues.
Vasoactive peptide is produced in many tissues including the gut, pancreas, and suprachiasmatic nuclei of the hypothalamus in the brain.
It stimulates contractility of heart, causes vasodilation, lowers arterial blood pressure.
Vasoactive peptide has a half-life (t½) in the blood of about two minutes.
Transgenic technology has established to be very useful for the functional analysis of vasoactive peptide systems.
A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.
Transgenesis is the process by which mixing up of genes takes place.
Foreign genes are inserted into the germ line of the animal, so it can be transmitted to the progeny.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.
First transgenic animal was a ‘Supermouse’ created by Ralph Brinster (U Pennsylvania) and Richard Palmiter (University of Washington) in 1982.
It was created by inserting a human growth hormone gene in mouse genome.
The offspring was much larger than the parents.
Mouse – common transgenic expt.
Other animals include pig, goat, cow, sheep, fish etc.
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptxMNAGAPRADHEESH
DEVELOPEMENT AND USES OF TRANSGENIC ANIMALS:
■Definitions about Transgenic Animals (or) Genetically Modified Animals(GMO).
■History and Developements of Transgenic Animals(Yearwise:1907-2017)
■Different Methods used for developement of Transgenic animals:
1.Microinjection Method
2.Retro Viral Method
3.Embryonic Stem cell method
■Applications of Transgenic Animals
■Advantages of Transgenic Animals
■Disadvantages of Transgenic Animals
■References.
☆GUYS,DOWNLOAD,SHARE LIKE ALL MY SLIDES AND GET BENEFIT FOR YOUR FUTURE RESEARCH AND ENDEAVOURS.
☆USEFUL ALL LIFE SCIENCES STUDENTS AND SCHOLARS.
transgenic animals , its production and applicationMonishaKCReddy
Process of introducing a foreign or exogenous DNA into an animal genome is called as Transgenesis
Transgenesis is the process of introducing an exogenous gene called a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
Retroviruses used as vectors to transfer genetic material into the host cell
Retroviruses can be used for the transfer of foreign genes into animal genomes.
Embryonic stem cell-mediated gene transfer.
Involves prior insertion of the desired DNA sequence by homologous recombination into an in vitro culture of embryonic stem (ES) cells. Incorporated into an embryo at the blastocyst stage of development.
This deals with transgenesis, history of transgenic animal, methodology , some examples of transgenic animals, importance, advantage and disadvantage of transgenic animals
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
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can be defined as
modification of the genetic
of an organism
through
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European Laboratory
Animal Associations
as an animal in which there has been a
deliberate modification of its,the
genetic
there has been a deliberate modification of its
genome
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•The genes can be modified to jump
wily-nilly around cells. By putting
them into a batch of
identify which genes or combinations of genes lead to cancer.
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Jumping genes from jelly
fish
•By putting them into a batch of
human or mouse cells, and watching
to see which ones become cancerous
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•1981: First transgenic mouse
–Insertion of hGH into a mouse (Singleton,
1999)
–Production of monoclonal antibodies &
anti-inflammatory agents
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2. An animal is given hormonal treatment to produce a large number
.
Of embryos, and the embryos are collected from the oviduct.
3. The human gene is inserted into the fertilized egg via
microinjection
4. The transgenic embryo is placed in a surrogate host which gives
Birth to the transgenic animal
5. The offspring is tested for the new gene
1. A human gene responsible for producing a desired
protein Is isolated in a laboratory
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transgenic
•gene transfer is the production of
transgenic dairy farm
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FDA, 2005
Mastitis Disease Resistance
Annie, born March 3, 2000, is a clone of a pure-bred Jersey calf
genes for Staphylococcus aurousbacteria
producing lysostaphin, a protein that kills
Staphylococcus aurousbacteria
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Lysozyme is a protein found in the tears
gene for an antibacterial enzyme found in human breast milk,
Lysozyme inhibits the growth of bacteria by destroying the bacterial cell wall
Treatment infant diarrhea
produce human lysozyme in their milk
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Transgenic goat
milk contains a protein that could be extracted
to make a drug for coronary bypass patients.
now in human clinical trials.
Millie is aHer The protein,, is
called anti-thrombin III
goat.
transgenic
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Steps
•the gene that produces AT III is
sequenced,.
build a synthetic gene and make many copies
and
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then
•The synthetic gene is then attached
to the gene forcasein,which acts as
apromoter gene.This ensemble is
then injected into a.
newly fertilized egg
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then
•. During the first few critical cell
divisions, the gene may become
attached to the goat's DNA.
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then
•If successful, the new gene is now a
transgenic which will become
incorporated into all cells during
subsequent divisions
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•Cloning through the ages:
–1952: Cloning via nuclear transfer (frog)
–1989-1990: First mammals cloned
–1995: First cloning via cultured
mammalian cells
–1997: First cloning via adult cells –
DOLLY
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history
•First cloning via transgenic adult cells – POLLY
–1998 – 2000: Cloning of cattle, pigs, mice,
goats and monkeys using adult cells
–2001: First reported cloned human embryo
–2002: First cloned pet
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How many genes are there
E. coli 4.6 Mb 4,288
genes
S. cerevisiae 13.5 Mb 6,034
genes
D. melanogaster 165 Mb 12,000
genes
C. elegans 97 Mb 19,099
genes
H. Sapiens 3,300 Mb 40,000
genes
Phenotypes
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I mean
•Animal model for disease
•Bioreactor for producing
•Therapeutic in milk of
•Goat
•rabbit
•Cow
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Transgenic animals are costly:
20,000-30,000 for one
animal,
and low chances of success. If
successful, one animal can
produce
in its life time 200,000-
300,000 million worth of
drugs.
A herd of 600 transgenic cows
could supply the worldwide
demand
of human serum albumin (used
in the treatment of burns
and traumatic injuries)
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Mention what is in your
list?
•Transgenic
•Transgenic mouse
•Gene expression regulation
•Structure function
•Cause and effect
•In path physiological process
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Mention what are in your
list
•Transgenic mouse
•Transgenic rat
•Transgenic rabbit
•Transgenic goat
•Transgenic chicken
•Transgenic cow
•Transgenic chicken
Transgenic
quail
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Transgenic rabbit
•used as model for cardiovascular
diseases
•Used as a model for AIDS
•Used as model for cancer
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Transgenic rabbit
•The recombinant protein can be
produced
•low cost
•Wide scale
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What rabbit milk
?
•What is can be in rabbit milk
•One of the most attractive protein
•Is alfa glycosidase
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when multiple preferred
analysis be performed on
single sample
blood
chronic studies
long term monitoring
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Transgenic chicken
•to understand normal and abnormal
embryo development
limb deformities
spinal bifida
avian stem cells in embryos
Is the coming
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Egg
recombinant protein could yield up to a
gram or more of the protein in the
naturally sterile egg
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Protein of therapeutic
significance
•interferon beta-1a, in the whites of
eggs laid by transgenic hens using the
employs Lent Vector. Interferon-
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lentivirus
•lent viral vectors have the advantage
of infecting both dividing and non-
dividing cells.
•However, they retain stable
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lentivirus
Lentivirus can be pseudo typed with
ease to infect certain tissues and cell
lines
with greater efficiency. Made to accommodate expression
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lentivirus
•these viral vectors do not elicit
immune responsesin vivo
can be concentrated to titers of 10.
109 Cfu/ ml
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difficulty
•embryos contain about 50,000 cells
before the egg is laid
inserting DNA into just one cell.
gene transfer in other mammals involves
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advantages
•chicken's generation time is around 6
months
shorter than the generation time of a large mammal
, such as a goat, which requires 18 months
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Advantage of egg white
•eggs a hen lays annually 330 eggs
contains
•6.5grams of various proteins
advantage of producing pharmaceuticals in eggs is that egg
Is
simpler
Than
milk
milk
Egg
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Egg white advantages
methods for easily extracting various
proteins from eggs.
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transgenic
Other important fields
neurobiology model
cardiovascular biology
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The MOUSE Life span: approx. 2.5 years
Gestation : 21 days
Litter size: 8 to 12
Generation time: three months
Several inbred and outbreed strains
Genomic database
Most advance genetic technologies
Cost per mouse 5 E p
Housing cost
Over 90% identical to human genome
Large enough for physiological studies
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NON-TRANSGENIC
TRANSGENIC
No modifications to the genome.
Modifications to the genome.
Germ line mutations Somatic cell mutations
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Special facilities
•Specialized glassware
Pulled, Holding and Transfer pipettes are used
for manipulating and moving embryos around.All are
handmade from stock glass tubing
Injection pipettes are machine pulled.
•Microscopes - Surgical, Dissecting, & Inverted
•Micromanipulators – Convert gross hand movements
into micro-movements
•Microsurgical instruments – Used for harvest and
re-implantation of embryos
•Incubator – Used when culturing embryos is required
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Microinjection Set-Up
Micromanipulator
Inverted Microscope
Hamilton Syringe
for operating
Holding Pipet Foot Pedal for operating
Microinjection Pipet
Nitrogen
Nitrogen Powered
Microinjection
Apparatus
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Pipets for Pronuclear Microinjection
Internal Diameter
120 m – 180 m
Internal Diameter
55 m – 70 m
Internal Diameter
120 m – 180 m
Pulled Holding Injection Transfer
Internal Diameter
1 m – 3 m
Next
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•Pregnant mare’s serum (PMSG)
is administered
intraperitoneally (I.P.) to donor
females
•PMSG mimics follicle
stimulating hormone and
induces the maturation of
oocytes in the ovary
Superovulation
Part I
Next
Day -3
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super ovulation
•Super ovulate donor females to
maximize embryo yield
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Day -1
•Human Chrionic Gonadotropin
(HCG) is administered I.P. to donor
females
•HGC mimics Latinizing hormone
and stimulates the ovulation of the
mature oocytes from the ovary
Superovulation
Part II
Next
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•Set-up mating of donor male and
female mice and recipient
females with vasectomized males
Donor females are then
placed with donor males
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Embryo Harvest
An abdominal
incision is made and
the ovaries, oviducts
and uteri are
excised
Next
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Ovary Fat Pad
Oviduct
Uterus
Isolating the Oviduct
The structures of the excised portion of the
reproductive tract are identified and the mesentery
torn away to facilitate isolation of the oviduct
Mesentery
Next
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Isolating the Oviduct
The oviduct, along with a small portion
of the uterus is isolated and removed
Next
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Fat pad
Oviduct
Freeing Embryos from Oviduct
Cumulus MassCumulus Mass
The cumulus mass is visualized through the wall of the oviduct
and a needle is used to tear it open and free the embryos
Next
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Media with hyaluronidase
Wash and Grade Embryos
The cumulus mass is placed in a large drop of medium with
hyalurdonidanse. After the cumulus cells have fallen away, the embryos
are transferred through three small rinse drops and graded for quality. Only
embryos suitable for injection advance to the final drop.
Rinse Drops
Embryos for injection
Next
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Grading Embryos
•Healthy, fertilized embryos must be
separated from degenerate and unfertile
embryos for injection
Next
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Zona Pellucida
Embryo
Pronuclear
Polar Bodies
Basic Embryo Anatomy
Next
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Embryos in Injection Dish
Acrylic Frame Glass Cover Slip
Embryo injections are performed on a special microscope slide
composed of an acrylic frame with a glass cover slip attached.
Embryos are placed in the center of a drop of medium that is
covered with oil to prevent movement and dehydration of the drop.
Next
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grading
•Characteristics of a good embryo:
–2 visible pronuclear
–Embryo should fill the zone
pellucida
–Well defined embryo within the
zona pellucida
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implantation
•Surgically re-implant injected
embryos into pseudo pregnant
recipients
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Pronuclear Microinjection
Using gentle suction, the embryos are held in place by the
holding pipet so that the pronucleus can be injected
Holding Pipet
Next
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Embryo Transfer
• After all embryos have been injected they are inspected and the number of embryos that still appear healthy are
separated for injection. Embryos which did not survive the injection process are discarded
• Using a remote mouth pipe ting device, embryos are loaded into a transfer pipette for re-implantation into
pseudopregnant recipients
• 20-30 embryos will be transferred into one oviduct of each recipient
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Loading the Transfer Pipet
Mineral Oil Air
Embryos in media
Alternate Transfer Pipet Design
The transfer pipette is filled with medium then loaded with the
embryos to be re-implanted in oviduct of the recipient female
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An incision is made in the flank of an
anesthetized mouse
The ovary and oviduct are
then gently pulled through
the incision. A tear is made
in the bursa and the
infundibulum exposed.
The tip of the loaded
transfer pipette is placed
inside the infundibulum and
the embryos gently blown
into the oviduct
The tract is returned to the
abdomen and the incision
site is closed.
Oviduct Transfer