Transgenic animals are created by introducing genes from other species into their genomes. This is done by microinjecting a cloned gene into a fertilized egg, implanting the egg into a female, and breeding the offspring to establish new genetic lines. Transgenic animals are produced for various purposes like studying gene expression and function, producing pharmaceutical proteins from milk or other tissues, and creating disease models for research. The mouse was the first transgenic animal created using microinjection techniques that are now commonly used to generate transgenic lines for research.
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
Introduction.
Definition.
Importance of transgenic animals.
Transgenic mice
Methods for introducing a foreign gene:
The retroviral vector method
The DNA microinjection method/ pronuclear microinjection
Genetically engineered embryonic stem cells
Transgenic fish
What is transgenic fish?
A few facts to know to know about transgenic fish.
Important points needed for genetic engineering (gene transfer) to produce transgenic fish.
Development of transgenic fishes.
A few examples
Auto-transgenesis.
Controlled culture of transgenic fish and feed.
Gene transfer technology for development of transgenic fishes.
Gene flow.
Food safety issues.
Conclusion.
Bibliography.
its a good type of ppt for understanding cloning and its types. It also enlists the idea of procedure in the lab to initiate initial division of embryo by electric current.
transgenic animals , its production and applicationMonishaKCReddy
Process of introducing a foreign or exogenous DNA into an animal genome is called as Transgenesis
Transgenesis is the process of introducing an exogenous gene called a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
Retroviruses used as vectors to transfer genetic material into the host cell
Retroviruses can be used for the transfer of foreign genes into animal genomes.
Embryonic stem cell-mediated gene transfer.
Involves prior insertion of the desired DNA sequence by homologous recombination into an in vitro culture of embryonic stem (ES) cells. Incorporated into an embryo at the blastocyst stage of development.
Introduction.
Definition.
Importance of transgenic animals.
Transgenic mice
Methods for introducing a foreign gene:
The retroviral vector method
The DNA microinjection method/ pronuclear microinjection
Genetically engineered embryonic stem cells
Transgenic fish
What is transgenic fish?
A few facts to know to know about transgenic fish.
Important points needed for genetic engineering (gene transfer) to produce transgenic fish.
Development of transgenic fishes.
A few examples
Auto-transgenesis.
Controlled culture of transgenic fish and feed.
Gene transfer technology for development of transgenic fishes.
Gene flow.
Food safety issues.
Conclusion.
Bibliography.
its a good type of ppt for understanding cloning and its types. It also enlists the idea of procedure in the lab to initiate initial division of embryo by electric current.
transgenic animals , its production and applicationMonishaKCReddy
Process of introducing a foreign or exogenous DNA into an animal genome is called as Transgenesis
Transgenesis is the process of introducing an exogenous gene called a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
Retroviruses used as vectors to transfer genetic material into the host cell
Retroviruses can be used for the transfer of foreign genes into animal genomes.
Embryonic stem cell-mediated gene transfer.
Involves prior insertion of the desired DNA sequence by homologous recombination into an in vitro culture of embryonic stem (ES) cells. Incorporated into an embryo at the blastocyst stage of development.
description of transgenic animals and production with desired traits using different methods and their applications and their advantages and disadvantages
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptxMNAGAPRADHEESH
DEVELOPEMENT AND USES OF TRANSGENIC ANIMALS:
■Definitions about Transgenic Animals (or) Genetically Modified Animals(GMO).
■History and Developements of Transgenic Animals(Yearwise:1907-2017)
■Different Methods used for developement of Transgenic animals:
1.Microinjection Method
2.Retro Viral Method
3.Embryonic Stem cell method
■Applications of Transgenic Animals
■Advantages of Transgenic Animals
■Disadvantages of Transgenic Animals
■References.
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This deals with transgenesis, history of transgenic animal, methodology , some examples of transgenic animals, importance, advantage and disadvantage of transgenic animals
This presentation aims to provide an in-depth understanding of the science behind creating transgenic animals, explore their potential applications, and delve into the ethical considerations surrounding this emerging field of research.
Definition and Background:
We begin by defining transgenic animals as organisms that have had their genetic material intentionally altered through the introduction of foreign genes. This groundbreaking field of genetic engineering has its roots in the development of recombinant DNA technology in the 1970s, which enabled the transfer of genes across different species.
Genetic Engineering Techniques:
This section delves into the techniques employed to create transgenic animals, emphasizing the following key methodologies:
a. DNA Microinjection: The introduction of foreign DNA into the pronucleus of a fertilized embryo, allowing the foreign gene to be incorporated into the animal's genome and expressed in its cells.
b. Gene Targeting: The precise modification of an organism's genome by replacing or disrupting specific genes using technologies such as homologous recombination or CRISPR-Cas9.
c. Somatic Cell Nuclear Transfer (SCNT): The cloning technique involving the transfer of a nucleus from a somatic cell into an enucleated egg, resulting in the creation of an embryo with the same genetic makeup as the somatic cell donor.
Applications of Transgenic Animals:
This section explores the wide-ranging applications of transgenic animals across various fields, including:
a. Biomedical Research: Transgenic animals serve as invaluable models for studying human diseases and testing potential therapies, enabling significant advancements in medical research.
b. Agriculture: Transgenic animals can be engineered to possess desirable traits, such as increased resistance to diseases or improved meat quality, offering the potential to enhance agricultural productivity and sustainability.
c. Pharmaceutical Production: Transgenic animals can be designed to produce therapeutic proteins or antibodies in their milk or blood, providing a cost-effective means of manufacturing valuable pharmaceutical products.
d. Organ Transplantation: Research on transgenic animals has explored the possibility of generating organs that are genetically compatible with humans, addressing the shortage of donor organs for transplantation.
Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
In silico drugs analogue design: novobiocin analogues.pptx
transgenic animals
1.
2. A transgenic animal is one whose genome has been changed to
carry genes from other species. The nucleus of all cells in
every living organism contains genes made up of DNA. These
genes store information that regulates how our bodies form
and function.
3. Man has always been trying to improve several traits in
domesticated animals, such as milk yeild,wool characterstics,
weigh gain, and egg laying frequency. the process included
selective breeding and and selection through several
generations. It was quite Successful. and time consuming and
very expensive . However ,recent devolpment in genetic
engneering have made it possible to introduce specific genes
into animals , and to create genetically altered or transgenic
animals with improved or new chacteristics
4. A cloned gene is injected into the nucleus of a fertilized egg
Fertilized egg, after injection of gene is implanted into the
uterus of a receptive female.
The progeny derived from the implanted egg carry the cloned
gene in all their cells.
Animals with the cloned gene integrated into their germ line
cells are bred to establish new genetic lines
Thus a number of transgenic animals are produced
5. • The first transgenic animals were mice created by Rudolf
Jaenisch in 1974. Jaenish successfully managed to insert
foreign DNA into the early-stage mouse embryos; the
resulting mice carried the modified gene in all their tissues.
6. • To study promoter function ,reporter gene expression and
functions of transferred genes
• To produce new proteins from cultured cell lines ,by the
insertion and expression of genes coding for specific proteins
• To create transgenic animals with higher yield of milk or meat
• To obtain better quality milk ,meat ,wool , etc
• To produce transgenic animals with the capacity to synthesize
new proteins .such animals are used in gene Pharming
(Pharming means to producing Pharmaceuticals .
• To use animals as models for studies in gene therapy
7. Gene pharming is a technology that scientists
use to alter an animal's own DNA, or to splice
in new DNA, called a transgene, from another
species. In pharming, these genetically
modified (transgenic) animals are used mostly
to make human proteins that have medicinal
value.
8. TRANSGENIC MICE
• The technology for producing transgenic animals
has been perfected in the laboratory mouse
,foreign DNA can be introduced into mice by the
following method
• Using retroviral vectors that infect the cells of an
early stage embryo, prior to implantation into a
receptive female
• microinjection into the enlarged sperm nucleus
• Introduction of genetically engineered embryonic
stem cells into the early stage developing embryo
of female
9. DNA MICRO INJECTION METHOD
• This is currently the preferred method for producing the transgenic
mice this process involves many steps
• To inoculate fertilised eggs there is need to have enough eggs the
donor females are ,therefore ,stimulated to superovulate
• The females are given an injection of pregnant maresserum and
another injection after 48 hrs,of human chorionic gonadotropin
• The normal mouse produces 5-10 eggs, but the superovulated
mouse produces 35-40 eggs
• The super ovulated are mated and the fertilized eggs are taken
from their oviducts
• Immediately after their collection ,the fertilized eggs are
microinjected with the transgene construct
10.
11.
12.
13. After inoculation ,25-30eggs are implanted into afoster
mother,however this process prepares the females uterus for
receiving eggs .copulation is the only way to prepare the uterus
for implantation after implantation of the microinjected fertilized
egg,the foster mother will deliver and release the pops in around
3 weeks
14. CLONING BY NUCLEAR TRANSFER
• A nucleus from a cultured cell can be transferd to an
enucleated egg and implanted into the uterus of a receptive
female animal. the egg will develop into embryo and finally
into offspring . if the nucleus is derived from the same animal
,the progeny will be clone offspring will be the the replica off
mother .the methodology was used for the much publicized
production of a sheep called dolly
15. IMPORTANCE OF TRANSGENIC MICE
Short oestrous cycle
Short gestation period
Relatively short genaration time
Production of several offspring per progeny
Ease of in vitro fertilization and in vitro embryo culture
Availabilty of a large number of genetic varieties
17. NUCLEAR TRANSFER IN SHEEP
• The nucleus of an ovum is removed with a pipette
• Cells from mammary epithelium of adult and the state is
induced by inhbiting cell growth
• The re nucleated ovum is grown in culture
• Then it is implanted into a foster mother ,where the
development proceeds to term
• The foster mother gives birth to transgenic lamb(young
sheep)
19. TRANSGENIC SHEEP APPLICATIONS
• Some of the pharmaceutical products produced in the milk of
transgenic sheep are
• Clotting factors
• Soluble cd4 protein
• Lactoferin
• Urokinase
• CFTR
• Interleukin-2
• And high rate of milk,wool and meat yielding is seen
20.
21. Other transgenic animals
• TRANSGENIC RABBIT
Rabbits are promissing for gene pharming for the production of
pharmacuetically or important protiens encoded by the
transgenes .the protein product is easily harvested with from
which it is conveniently purified
22. Transgenic cattle
• The only successful transfection technique in cattle is
microinjesction of fertilized ova which may either be
recovered surgically in vitro
• Increased milk rate
• pharming
23.
24.
25. TRANSGENIC PIGS
Pigs have been viewed as great experiment al animals for
transgenic research .first of all the human haemoglobin gene
have been expressed in pigs
Transgenic pigs have recently found favour as research materials
to produce organs for human transplants as human organs
are in short supply .pigs can be used as donors of kidney heart
etc. however due to hypercute rejection of foreign organs in
the human system .
The research in the direction has, again , along way to go
35. Questions on transgenic animals
1. Describe the application of transgenic
animals?
2. Discuss the DNA microinjection method is
carried out in producing transgenic mice?
3. What is meant by gene pharming?