Genetically Modified Organisms
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The document discusses transgenic animals, which are organisms that have had foreign genes inserted into their genomes. The first transgenic animal was a mouse created in 1982. The document outlines the process of creating transgenic animals, including constructing a transgene and introducing it into an animal's genome via pronuclear microinjection or stem cell methods. Potential issues and applications are also mentioned, such as using transgenic animals for pharmaceutical production, agriculture, and disease modeling.
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Transgenic animal production and its application
A genetically modified animal with the heterologous gene of interest being inserted for the purpose of biopharming or make a diseased model to study the consequences of disease and its probable therapy
Transgenic animals (1) panjab university
This document discusses transgenic animals. It defines transgenic animals as animals whose genomes have been altered by transferring genes from another species or breed. It then discusses the process of transgenesis and some reasons for using transgenic technology, such as generating disease models and producing therapeutic proteins. The document provides a brief history of transgenic animals and explains why mice are commonly used. It outlines methods for generating transgenic animals like microinjection and viral infection. It also discusses applications of transgenic animals like studying gene function and developing disease models. In conclusion, the document notes some ethical issues around transgenic animals.
Transgenic animals
Transgenic animals are organisms that have been genetically engineered to carry foreign DNA in their genome. This document discusses transgenic animals, including their definition, goals, benefits and risks, types, methods of production, and applications. Some key points covered are: transgenic animals are useful for studying gene function and producing human proteins; common types include mice, fish, cows, and pigs; methods to create them include pronuclear microinjection and using embryonic stem cells or retroviruses; they have applications in research, agriculture, and biotechnology.
STRUCTURAL GENOMICS, FUNCTIONAL GENOMICS, COMPARATIVE GENOMICS
Structural genomics is a field that aims to determine the 3D structures of all proteins encoded by a genome. It involves determining structures on a large scale using techniques like X-ray crystallography and NMR. This allows identification of novel protein folds and potential drug targets. Comparative genomics compares genomic features between organisms and provides insights into evolution and conserved sequences and functions. It is a key tool in fields like medicine and agriculture.
Genome sequencing
The document discusses genome sequencing and related topics. It begins by defining what a genome is - the complete set of DNA in an organism. It then discusses the different types of genomes, such as prokaryotic and eukaryotic, including nuclear, mitochondrial, and chloroplast genomes. The document also defines genomics as the comprehensive study of whole genomes and all gene interactions, distinguishing it from traditional genetics which focuses on single genes. It outlines some key milestones in genomic sequencing and the technical foundations that enabled sequencing whole genomes. Finally, it describes the main approaches used for genome sequencing projects, including hierarchical shotgun sequencing and whole genome shotgun sequencing.
Genomics and proteomics (Bioinformatics)
Genomics is the study of genomes, including sequencing genomes and determining the complete set of proteins and genes in an organism. The first genomes sequenced included Haemophilus influenzae in 1995 and the human genome was completed in 2003, taking 13 years. Genomics provides information on genes, metabolic pathways, and the functioning of organisms through approaches like genome sequencing, structural genomics, functional genomics, comparative genomics, and proteomics.
Transgenic animals, mice and fish
The document discusses transgenic mice and fish. It defines transgenic animals as those permanently engineered through gene insertion. Methods to create transgenic mice include retroviral vectors, DNA microinjection into pronuclei, and using engineered embryonic stem cells. Transgenic mice have aided research in many areas. Transgenic fish are also discussed, including important salmon and tilapia examples. Gene flow and food safety are concerns for transgenic animals.
Method of gene transfer
Transfection involves introducing foreign DNA into host cells to produce a new phenotype. There are two main methods of transfection - vector-mediated and non-vector mediated. Vector-mediated transfection uses bacteriophage, retroviral, cosmid, baculovirus, and plasmid vectors to introduce DNA. Non-vector mediated methods include direct techniques like microinjection, electroporation, and particle bombardment, and indirect techniques like calcium phosphate precipitation and DEAE-dextran. Retroviral vectors are modified retroviruses that can introduce foreign DNA into host chromosomal DNA. Microinjection involves injecting DNA directly into cells using a micropipette under a microscope. Electroporation uses electric pulses to create temporary
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Transgenic animal production and its application
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This document discusses transgenic animals. It defines transgenic animals as animals whose genomes have been altered by transferring genes from another species or breed. It then discusses the process of transgenesis and some reasons for using transgenic technology, such as generating disease models and producing therapeutic proteins. The document provides a brief history of transgenic animals and explains why mice are commonly used. It outlines methods for generating transgenic animals like microinjection and viral infection. It also discusses applications of transgenic animals like studying gene function and developing disease models. In conclusion, the document notes some ethical issues around transgenic animals.
Transgenic animals
Transgenic animals are organisms that have been genetically engineered to carry foreign DNA in their genome. This document discusses transgenic animals, including their definition, goals, benefits and risks, types, methods of production, and applications. Some key points covered are: transgenic animals are useful for studying gene function and producing human proteins; common types include mice, fish, cows, and pigs; methods to create them include pronuclear microinjection and using embryonic stem cells or retroviruses; they have applications in research, agriculture, and biotechnology.
STRUCTURAL GENOMICS, FUNCTIONAL GENOMICS, COMPARATIVE GENOMICS
Structural genomics is a field that aims to determine the 3D structures of all proteins encoded by a genome. It involves determining structures on a large scale using techniques like X-ray crystallography and NMR. This allows identification of novel protein folds and potential drug targets. Comparative genomics compares genomic features between organisms and provides insights into evolution and conserved sequences and functions. It is a key tool in fields like medicine and agriculture.
Genome sequencing
The document discusses genome sequencing and related topics. It begins by defining what a genome is - the complete set of DNA in an organism. It then discusses the different types of genomes, such as prokaryotic and eukaryotic, including nuclear, mitochondrial, and chloroplast genomes. The document also defines genomics as the comprehensive study of whole genomes and all gene interactions, distinguishing it from traditional genetics which focuses on single genes. It outlines some key milestones in genomic sequencing and the technical foundations that enabled sequencing whole genomes. Finally, it describes the main approaches used for genome sequencing projects, including hierarchical shotgun sequencing and whole genome shotgun sequencing.
Genomics and proteomics (Bioinformatics)
Genomics is the study of genomes, including sequencing genomes and determining the complete set of proteins and genes in an organism. The first genomes sequenced included Haemophilus influenzae in 1995 and the human genome was completed in 2003, taking 13 years. Genomics provides information on genes, metabolic pathways, and the functioning of organisms through approaches like genome sequencing, structural genomics, functional genomics, comparative genomics, and proteomics.
Transgenic animals, mice and fish
The document discusses transgenic mice and fish. It defines transgenic animals as those permanently engineered through gene insertion. Methods to create transgenic mice include retroviral vectors, DNA microinjection into pronuclei, and using engineered embryonic stem cells. Transgenic mice have aided research in many areas. Transgenic fish are also discussed, including important salmon and tilapia examples. Gene flow and food safety are concerns for transgenic animals.
Method of gene transfer
Transfection involves introducing foreign DNA into host cells to produce a new phenotype. There are two main methods of transfection - vector-mediated and non-vector mediated. Vector-mediated transfection uses bacteriophage, retroviral, cosmid, baculovirus, and plasmid vectors to introduce DNA. Non-vector mediated methods include direct techniques like microinjection, electroporation, and particle bombardment, and indirect techniques like calcium phosphate precipitation and DEAE-dextran. Retroviral vectors are modified retroviruses that can introduce foreign DNA into host chromosomal DNA. Microinjection involves injecting DNA directly into cells using a micropipette under a microscope. Electroporation uses electric pulses to create temporary
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Transgenic animals by Kashikant Yadav
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PCR
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Transgenic animals ppt
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Transgenic technology
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Transgenic Animals
Transgenesis involves introducing foreign DNA into an animal's genome. This allows for the production of transgenic animals that exhibit new traits. Common methods for creating transgenic animals include pronuclear microinjection, embryonic stem cell manipulation, and retrovirus-mediated gene transfer. Examples of transgenic animals include glowing fish, disease models like Alzheimer's mice, and farm animals engineered for increased wool/milk. While transgenic technology has benefits for research, agriculture, and medicine, it also carries some risks that require further study.
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Introduction
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Transgenic mice
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Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
Transgenic animals
This document discusses the creation of transgenic animals and cloning. It provides details on the four main routes to create transgenic mammals: microinjection of DNA, integration of viral vectors, incorporation of stem cells, and nuclear transfer. For each method, it describes the key steps and challenges. The document also covers various applications of transgenic animals like producing human therapeutic proteins in the milk of livestock. Overall, it serves as a comprehensive overview of generating transgenic animals and the techniques involved.
Animal models
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Nested pcr
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Transgenic animals are produced by inserting foreign genes into their genomes using recombinant DNA methodology. This allows for increased growth, improved disease resistance, and other benefits. However, it can also lead to unintended effects if the inserted gene has multiple functions or causes mutations. Common methods to create transgenic animals include embryonic stem cell methods, pronuclear injection, and retrovirus-mediated gene transfer. Examples include transgenic mice, cows, fish, sheep, and monkeys.
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Transgenic animals
A transgenic animal is one that has had foreign DNA inserted into its genome. The first transgenic animal was a mouse created in 1982 by inserting a human growth hormone gene. Transgenic animals are created through pronuclear microinjection or stem cell methods. They have applications in medicine, agriculture, and industry. However, some argue that transgenic technology raises ethical issues.
Application of genomics in animals
Genomics is being widely applied in animal agriculture. It allows identification of genes associated with traits like disease resistance and production to select superior breeding animals. Techniques include cloning, genetic engineering of transgenic animals, and gene therapy. Genomic selection is revolutionizing livestock breeding by enabling early selection of breeding stock and improving genetic gains.
ABT Introduction
This document provides information about animal biotechnology through several sections. It begins with an introduction that discusses the long history of animal biotechnology including traditional breeding techniques dating back to 5000 BC. It then covers the history of animal biotechnology from the 1970s to the present day, highlighting important milestones. Several sections follow on the scope, applications, and terminology of animal biotechnology including transgenic animals, cloning, animal models in research, vaccines, nutrition, and embryo transfer. The document concludes by defining common terminology used in animal cell culture.
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Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
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Application of transgenic mice
Conclusion
References
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This document discusses the creation of transgenic animals and cloning. It provides details on the four main routes to create transgenic mammals: microinjection of DNA, integration of viral vectors, incorporation of stem cells, and nuclear transfer. For each method, it describes the key steps and challenges. The document also covers various applications of transgenic animals like producing human therapeutic proteins in the milk of livestock. Overall, it serves as a comprehensive overview of generating transgenic animals and the techniques involved.
Animal models
This document defines animal models and discusses their use in research. It defines an animal model as a living organism that resembles a pathological process in humans. Major advances involving animal models are described from the 1600s to present day, including vaccination and understanding of infectious disease. Common animal models used are then outlined, including rodents like rats, mice and guinea pigs as well as non-rodents like rabbits, monkeys, cats and dogs. The document discusses specific uses and characteristics of different animal models and alternatives to animal testing.
Gene sequencing methods
This document discusses various gene sequencing methods. It begins by introducing DNA and the importance of sequencing the genetic code. It then describes several early sequencing techniques like Sanger sequencing using chain termination or chemical cleavage. It discusses the need for sequencing to understand genetic conditions. The document also covers topics like genome sequencing, genomics, and high-throughput sequencing techniques like dye-terminator sequencing which replaced radioactive labels with fluorescent labels to automate the process.
Nested pcr
Nested PCR is a modification of conventional PCR that uses two sets of primers to improve sensitivity and specificity. It involves two rounds of amplification, where the first round uses outer primers that bind outside the target DNA to amplify a larger fragment. The second round uses inner primers that bind within the first amplified fragment to specifically amplify the target DNA. This blocks non-specific amplification. Nested PCR allows for the accurate detection of pathogens or genes present at low levels. While more sensitive and specific than conventional PCR, it is also more time-consuming and prone to contamination due to the use of two primer sets.
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Transgenic animals are genetically engineered to contain extra genes. This seminar discusses methods for creating transgenic animals like microinjection, embryonic stem cell transfer, and nuclear transfer. Microinjection involves injecting foreign genes into fertilized eggs. Embryonic stem cell transfer modifies stem cells in culture before implanting in blastocysts. Nuclear transfer fuses donor cell nuclei with egg cells. Examples given include producing human proteins in cow's milk and human hemoglobin in pigs. While transgenic animals could benefit humans, the process is inefficient and risky for animal health.
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Modification n animal genome transgenic animal useful fr get some valuable therapeutics model animals
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Transgenic animal models for the functional analysis of vasoactive peptides
The renin–angiotensin system (RAS) is a hormone system that regulates blood pressure and fluid balance.
renin–angiotensin system (RAS) is developed in animal by transgenic technology and effects of vasoactive peptide are seen.
Vasoactive peptide is a peptide hormone containing 28 amino acid residues.
Vasoactive peptide is produced in many tissues including the gut, pancreas, and suprachiasmatic nuclei of the hypothalamus in the brain.
It stimulates contractility of heart, causes vasodilation, lowers arterial blood pressure.
Vasoactive peptide has a half-life (t½) in the blood of about two minutes.
Transgenic technology has established to be very useful for the functional analysis of vasoactive peptide systems.
presentation on transgenic animals
Refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation.
Foreign DNA is introduced into the animal, using recombinant DNA technology,
Transgenic animals new
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Transgenic animal
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
KnockOut mouse technology By Bikash karki
The document summarizes the process of creating a knockout mouse through genetic engineering techniques. Key points:
- Knockout mice are created by "knocking out" or inactivating specific genes in embryonic stem cells taken from early mouse embryos.
- There are two main methods - homologous recombination, which precisely replaces a gene with an inactive version, and gene trapping, which randomly inserts DNA to disrupt gene function.
- Genetically modified stem cells are injected into mouse blastocysts to generate chimeric mice, and breeding is used to produce mice that are homozygous for the knocked out gene. Studying these mice helps reveal the function of the targeted gene.
Transgenic animals - A brief review
Transgenic animals are animals whose genomes have been altered by the addition of foreign DNA. There are three main methods for creating transgenic animals: retroviral vector method, DNA microinjection, and using engineered embryonic stem cells. Many transgenic animals have been created successfully for various purposes, including glowing zebrafish, faster growing salmon, Alzheimer's disease mouse models, and the first transgenic monkey. Transgenic technology holds promise for applications in agriculture, medicine, and industry, but also raises ethical concerns and biosafety issues.
Transgenic animals
Transgenic animals are animals whose DNA has been altered by the addition of foreign genes that induce the expression of new or modified traits. Key methods for creating transgenic animals include microinjection of DNA into fertilized eggs and embryonic stem cell manipulation. Transgenic animals have various applications including serving as disease models, producing pharmaceuticals in their milk (transpharming), and providing organs/tissues for transplantation (xenotransplantation). While transgenic research holds promise for advancing medicine and agriculture, it also raises ethical issues regarding animal welfare and unintended environmental consequences. Oversight aims to ensure research is conducted humanely.
Transgenic Animals
This document discusses various methods for creating transgenic animals. Nuclear microinjection involves injecting a transgene into fertilized egg cells, which are then cultured and implanted into a foster mother. This can result in the stable integration of the transgene into the founder animal's genome. Embryonic stem cells can also be engineered with a transgene and inserted into a blastocyst to generate a chimeric transgenic animal. Precise targeting of transgenes utilizes homologous recombination to replace the native gene with an altered version at the same chromosomal location. Position effects can influence transgene expression depending on the location of integration in the host genome.
Transgenic organisms powerpoint
A transgenic animal is one that has had part of another species' genome transferred into its own through genetic engineering techniques. One common transgenic animal is mice. To create a transgenic mouse, scientists typically microinject a transgene into fertilized mouse eggs which are then implanted into a foster mother mouse. The offspring are tested for the presence of the transgene. Transgenic mice are useful for studying diseases and testing toxicants. While they aid research, some have ethical concerns about transgenic animal welfare and environmental impacts if genetically modified animals escape.
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Transgenic animals
Transgenic animals are created through recombinant DNA technology by inserting foreign genes into the animal's genome. This is done to improve livestock, use animals as bioreactors for pharmaceutical production, and for research purposes. The main methods of creating transgenic animals are DNA microinjection, retrovirus-mediated gene transfer, embryonic stem cell transfer, and sperm-mediated gene transfer. Examples include transgenic cows that produce more nutritious milk, pigs with genes to reduce environmental pollution, and mice used widely as model organisms. While transgenic animals have benefits, there are also ethical concerns regarding animal welfare and unintended environmental impacts.
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This document discusses various genetically modified animals and their uses. It describes how transgenic fruit flies are used in biological research to study the effects of genetic changes on development. It also discusses how genetically modified mosquitoes have been developed to combat the spread of malaria and dengue fever by containing a lethal gene. Finally, it summarizes that genetically modified animals have medical, agricultural, and industrial importance, including uses as disease models, producing pharmaceuticals, improving food production, and testing chemical toxicity.
Transgenic animals by Ashish
Transgenic animals are created by inserting foreign genes into the animal's genome. The first transgenic animal was a "Supermouse" created in 1982. There are several methods to produce transgenic animals, including pronuclear microinjection, embryonic stem cell methods, sperm-mediated transgenesis, and somatic cell nuclear transfer. Transgenic animals have applications in medicine, agriculture, and industry. However, there are also some ethical and environmental concerns regarding transgenic technology.
Transgenic Animals
This document discusses transgenic animals. It defines transgenic animals as those with foreign genes deliberately inserted into their genomes. It describes the three main methods for creating transgenic animals: retrovirus-mediated gene transfer, DNA microinjection, and embryonic stem cell-mediated gene transfer. Examples are given of transgenic animals created for medical, agricultural, and industrial purposes, such as disease-resistant livestock, bioreactors that produce useful proteins, and fish engineered for rapid growth. Both the promises and ethical concerns of transgenic technology are acknowledged.
PRESENTATION ON TRANSGENIC ANIMALS AND ITS APPLICATION.pptx
This deals with transgenesis, history of transgenic animal, methodology , some examples of transgenic animals, importance, advantage and disadvantage of transgenic animals
TRANSGENIC ANIMALS AND ITS APPLICATION
Transgenesis, history, some examples of transgenic animals, methodology of production, it's advantage ad disadvantage
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Transgenic animals are produced by introducing foreign DNA into an animal's genome. The first transgenic animal was a mouse created in 1974. Since then, various methods have been used to generate transgenic fish, livestock, and other species. Transgenic animals have applications in biomedical research, agriculture, and industry. They can serve as models for human disease or help produce pharmaceuticals in their milk. However, transgenesis also carries risks if the inserted gene has unintended effects on the animal's development or physiology.
Presentation 25th aug 2014
Transgenic animals are produced by inserting foreign DNA into the genome of an animal. The first transgenic animals were mice created in 1974 by injecting foreign DNA into mouse embryos. The presentation discusses the history of transgenic animals and the process used to produce them, including microinjection of DNA into fertilized eggs and using retroviruses. Advantages include improved traits for research, agriculture, and producing human proteins. Concerns include potential human health impacts and effects on the environment.
TRANSGENIC ANIMAL.pptx
Transgenic animals are created by inserting foreign genes into an animal's genome. The first transgenic animal was a mouse created in 1982 by inserting a human growth hormone gene. There are two main methods for creating transgenic animals - pronuclear microinjection and embryonic stem cell manipulation. Transgenic animals have applications in medicine as disease models, in agriculture to improve crop yields, and in industry for toxicity testing. They require careful maintenance and ethical oversight.
TRANSGENIC ANIMAL.pptx
Transgenic animals are created by inserting foreign genes into an animal's genome. The first transgenic animal was a mouse created in 1982 by inserting a human growth hormone gene. There are two main methods for creating transgenic animals - pronuclear microinjection and embryonic stem cell manipulation. Transgenic animals have applications in medicine as disease models, in agriculture to improve crop yields, and in industry for toxicity testing. They require careful maintenance and ethical oversight.
Transgenic animals
Transgenic animals are genetically modified organisms with DNA from another source inserted into their genome. The document discusses the history of studying genes and developing transgenic techniques. It provides details on how transgenic animals are produced, primarily through DNA microinjection into reproductive cells. A variety of transgenic animals have been created for various purposes, such as developing disease models. While transgenic research has benefits, it also raises ethical issues and animal welfare concerns that require consideration.
Transgenic animals
Transgenic animals are created through genetic engineering by introducing foreign genes into the animal's genome. This allows the animal to produce proteins it would not normally make. Methods for creating transgenic animals include microinjection of DNA into fertilized eggs or embryonic stem cells. Transgenic animals have various applications including serving as disease models, producing pharmaceuticals in their milk (transpharmers), providing organs or tissues for transplantation (xenotransplantation), and enhancing food production. However, transgenic animal research also raises ethical issues regarding animal welfare and the environmental impacts of genetic modification.
transgenic animals by apexa.pdf
Transgenic animals are created through manipulating an animal's DNA by introducing foreign genes. The first transgenic animals were mice created in 1974 by microinjecting genes into embryos. Common methods to create transgenic animals are DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals have applications in agriculture to improve traits like milk production, applications in medicine to produce pharmaceuticals, and industrial applications like producing spider silk proteins. While transgenic animals provide benefits, they also require special care and precautions to address potential health and breeding issues.
selective breeding and genetic engineering
Selective breeding and genetic engineering are techniques used to produce offspring with desirable traits. Selective breeding involves selecting animals with desired traits and breeding them, while genetic engineering transfers genes between organisms. Both techniques have benefits like increased crop yields or disease resistance, but also risks like loss of genetic diversity, animal health issues, or unintended effects. Genetic engineering in particular raises ethical concerns about manipulating natural organisms.
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptx
DEVELOPEMENT AND USES OF TRANSGENIC ANIMALS:
■Definitions about Transgenic Animals (or) Genetically Modified Animals(GMO).
■History and Developements of Transgenic Animals(Yearwise:1907-2017)
■Different Methods used for developement of Transgenic animals:
1.Microinjection Method
2.Retro Viral Method
3.Embryonic Stem cell method
■Applications of Transgenic Animals
■Advantages of Transgenic Animals
■Disadvantages of Transgenic Animals
■References.
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Transgenic Animals
Genetically modified Organism are organism that have had a foreign gene deliberately inserted into its genome
Transgenesis in animals
Introduction
History
Landmarks Events in Transgenic Livestock Research
Techniques/ Method for Gene Transfer
Examples of transgenesis
Importance
Application
Limitation
Issue related to Transgenic Technology
Ethical concerns and how to Overcome
transgenic animals , its production and application
Process of introducing a foreign or exogenous DNA into an animal genome is called as Transgenesis
Transgenesis is the process of introducing an exogenous gene called a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
Retroviruses used as vectors to transfer genetic material into the host cell
Retroviruses can be used for the transfer of foreign genes into animal genomes.
Embryonic stem cell-mediated gene transfer.
Involves prior insertion of the desired DNA sequence by homologous recombination into an in vitro culture of embryonic stem (ES) cells. Incorporated into an embryo at the blastocyst stage of development.
Similar to Genetically Modified Organisms (20)
PRESENTATION ON TRANSGENIC ANIMALS AND ITS APPLICATION.pptx
PRESENTATION ON TRANSGENIC ANIMALS AND ITS APPLICATION.pptx
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptx
Transgenic Animals developement and uses(M.NAGAPRADHEESH).pptx
transgenic animals , its production and application
transgenic animals , its production and application
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Recycling and Disposal on SWM Raymond Einyu pptx
Increasing urbanization, rural–urban migration, rising standards of living, and rapid development associated with population growth have resulted in increased solid waste generation by industrial, domestic and other activities in Nairobi City. It has been noted in other contexts too that increasing population, changing consumption patterns, economic development, changing income, urbanization and industrialization all contribute to the increased generation of waste.
With the increasing urban population in Kenya, which is estimated to be growing at a rate higher than that of the country’s general population, waste generation and management is already a major challenge. The industrialization and urbanization process in the country, dominated by one major city – Nairobi, which has around four times the population of the next largest urban centre (Mombasa) – has witnessed an exponential increase in the generation of solid waste. It is projected that by 2030, about 50 per cent of the Kenyan population will be urban.
Aim:
A healthy, safe, secure and sustainable solid waste management system fit for a world – class city.
Improve and protect the public health of Nairobi residents and visitors.
Ecological health, diversity and productivity and maximize resource recovery through the participatory approach.
Goals:
Build awareness and capacity for source separation as essential components of sustainable waste management.
Build new environmentally sound infrastructure and systems for safe disposal of residual waste and replacing current dumpsites which should be commissioned.
Current solid waste management situation:
The status.
Solid waste generation rate is at 2240 tones / day
collection efficiently is at about 50%.
Actors i.e. city authorities, CBO’s , private firms and self-disposal
Current SWM Situation in Nairobi City:
Solid waste generation – collection – dumping
Good Practices:
• Separation – recycling – marketing.
• Open dumpsite dandora dump site through public education on source separation of waste, of which the situation can be reversed.
• Nairobi is one of the C40 cities in this respect , various actors in the solid waste management space have adopted a variety of technologies to reduce short lived climate pollutants including source separation , recycling , marketing of the recycled products.
• Through the network, it should expect to benefit from expertise of the different actors in the network in terms of applicable technologies and practices in reducing the short-lived climate pollutants.
Good practices:
Despite the dismal collection of solid waste in Nairobi city, there are practices and activities of informal actors (CBOs, CBO-SACCOs and yard shop operators) and other formal industrial actors on solid waste collection, recycling and waste reduction.
Practices and activities of these actor groups are viewed as innovations with the potential to change the way solid waste is handled.
CHALLENGES:
• Resource Allocation.
Improving the Management of Peatlands and the Capacities of Stakeholders in I...
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The popularity of functional foods among scientists and common people has been increasing day by day. Awareness and modernization make the consumer think better regarding food and nutrition. Now a day’s individual knows very well about the relation between food consumption and disease prevalence. Humans have a diversity of microbes in the gut that together form the gut microflora. Probiotics are the health-promoting live microbial cells improve host health through gut and brain connection and fighting against harmful bacteria. Bifidobacterium and Lactobacillus are the two bacterial genera which are considered to be probiotic. These good bacteria are facing challenges of viability. There are so many factors such as sensitivity to heat, pH, acidity, osmotic effect, mechanical shear, chemical components, freezing and storage time as well which affects the viability of probiotics in the dairy food matrix as well as in the gut. Multiple efforts have been done in the past and ongoing in present for these beneficial microbial population stability until their destination in the gut. One of a useful technique known as microencapsulation makes the probiotic effective in the diversified conditions and maintain these microbe’s community to the optimum level for achieving targeted benefits. Dairy products are found to be an ideal vehicle for probiotic incorporation. It has been seen that the encapsulated microbial cells show higher viability than the free cells in different processing and storage conditions as well as against bile salts in the gut. They make the food functional when incorporated, without affecting the product sensory characteristics.
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Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
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Water polluted by dyestuffs compounds is a global threat to health and the environment; accordingly, we prepared a green novel sorbent chemical and Physical system from an algae, chitosan and chitosan nanoparticle and impregnated with algae with chitosan nanocomposite for the sorption of Malachite green dye from water. The algae with chitosan nanocomposite by a simple method and used as a recyclable and effective adsorbent for the removal of malachite green dye from aqueous solutions. Algae, chitosan, chitosan nanoparticle and algae with chitosan nanocomposite were characterized using different physicochemical methods. The functional groups and chemical compounds found in algae, chitosan, chitosan algae, chitosan nanoparticle, and chitosan nanoparticle with algae were identified using FTIR, SEM, and TGADTA/DTG techniques. The optimal adsorption conditions, different dosages, pH and Temperature the amount of algae with chitosan nanocomposite were determined. At optimized conditions and the batch equilibrium studies more than 99% of the dye was removed. The adsorption process data matched well kinetics showed that the reaction order for dye varied with pseudo-first order and pseudo-second order. Furthermore, the maximum adsorption capacity of the algae with chitosan nanocomposite toward malachite green dye reached as high as 15.5mg/g, respectively. Finally, multiple times reusing of algae with chitosan nanocomposite and removing dye from a real wastewater has made it a promising and attractive option for further practical applications.
Microbial characterisation and identification, and potability of River Kuywa ...
Microbial characterisation and identification, and potability of River Kuywa ...Open Access Research Paper
Water contamination is one of the major causes of water borne diseases worldwide. In Kenya, approximately 43% of people lack access to potable water due to human contamination. River Kuywa water is currently experiencing contamination due to human activities. Its water is widely used for domestic, agricultural, industrial and recreational purposes. This study aimed at characterizing bacteria and fungi in river Kuywa water. Water samples were randomly collected from four sites of the river: site A (Matisi), site B (Ngwelo), site C (Nzoia water pump) and site D (Chalicha), during the dry season (January-March 2018) and wet season (April-July 2018) and were transported to Maseno University Microbiology and plant pathology laboratory for analysis. The characterization and identification of bacteria and fungi were carried out using standard microbiological techniques. Nine bacterial genera and three fungi were identified from Kuywa river water. Clostridium spp., Staphylococcus spp., Enterobacter spp., Streptococcus spp., E. coli, Klebsiella spp., Shigella spp., Proteus spp. and Salmonella spp. Fungi were Fusarium oxysporum, Aspergillus flavus complex and Penicillium species. Wet season recorded highest bacterial and fungal counts (6.61-7.66 and 3.83-6.75cfu/ml) respectively. The results indicated that the river Kuywa water is polluted and therefore unsafe for human consumption before treatment. It is therefore recommended that the communities to ensure that they boil water especially for drinking.
Peatlands of Latin America and the Caribbean
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This presentation is for us to know that how our Environment need Attention for protection of our natural resources which are depleted day by day that's why we need to take time and shift our attention to renewable energy sources instead of non-renewable sources which are better and Eco-friendly for our environment. these renewable energy sources are so helpful for our planet and for every living organism which depends on environment.
Epcon is One of the World's leading Manufacturing Companies.
Epcon is One of the World's leading Manufacturing Companies. With over 4000 installations worldwide, EPCON has been pioneering new techniques since 1977 that have become industry standards now. Founded in 1977, Epcon has grown from a one-man operation to a global leader in developing and manufacturing innovative air pollution control technology and industrial heating equipment.
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
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本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
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一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Promoting Multilateral Cooperation for Sustainable Peatland management
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本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Results of geophysics and pneumatic injection pilot tests during 2003 – 2007 yielded significant positive results for injection delivery design and contaminant mass treatment, resulting in permanent shut-down of an existing groundwater Pump & Treat system.
Accessible source areas were subsequently removed (2011) by soil excavation and treated with the placement of Emulsified Vegetable Oil EVO and zero-valent iron ZVI to accelerate treatment of impacted groundwater in overburden and weathered fractured bedrock. Post pilot test and post remediation groundwater monitoring has included analyses of CVOCs, organic fatty acids, dissolved gases and QuantArray® -Chlor to quantify key microorganisms (e.g., Dehalococcoides, Dehalobacter, etc.) and functional genes (e.g., vinyl chloride reductase, methane monooxygenase, etc.) to assess potential for reductive dechlorination and aerobic cometabolism of CVOCs.
In 2022, the first commercial application of MetaArray™ was performed at the site. MetaArray™ utilizes statistical analysis, such as principal component analysis and multivariate analysis to provide evidence that reductive dechlorination is active or even that it is slowing. This creates actionable data allowing users to save money by making important site management decisions earlier.
The results of the MetaArray™ analysis’ support vector machine (SVM) identified groundwater monitoring wells with a 80% confidence that were characterized as either Limited for Reductive Decholorination or had a High Reductive Reduction Dechlorination potential. The results of MetaArray™ will be used to further optimize the site’s post remediation monitoring program for monitored natural attenuation.
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The incorporation of a 3DCSM and completion of HRSC provided a tool for enhanced, data-driven, decisions to support a change in remediation closure strategies. Currently, an approved pilot study has been obtained to shut-down the remediation systems (ISCO, P&T) and conduct a hydraulic study under non-pumping conditions. A separate micro-biological bench scale treatability study was competed that yielded positive results for an emerging innovative technology. As a result, a field pilot study has commenced with results expected in nine-twelve months. With the results of the hydraulic study, field pilot studies and an updated risk assessment leading site monitoring optimization cost lifecycle savings upwards of $15MM towards an alternatively evolved best available technology remediation closure strategy.
Climate Change All over the World .pptx
Climate change refers to significant and lasting changes in the average weather patterns over periods ranging from decades to millions of years. It encompasses both global warming driven by human emissions of greenhouse gases and the resulting large-scale shifts in weather patterns. While climate change is a natural phenomenon, human activities, particularly since the Industrial Revolution, have accelerated its pace and intensity
Wildlife-AnIntroduction.pdf so that you know more about our environment
All about wildlife, if you are fond of animals and wildlife then do follow us for more content like this
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Genetically Modified Organisms
- 1. Y13PH1224
- 2. INTRODUCTION • A TRANSGENIC ANIMAL IS ONE THAT CARRIES A FOREIGN GENE THAT HAS BEEN DELIBERATELY INSERTED INTO ITS GENOME. • TRANSGENESIS IS THE PROCESS BY WHICH MIXING UP OF GENES TAKES PLACE. • FOREIGN GENES ARE INSERTED INTO THE GERM LINE OF THE ANIMAL, SO IT CAN BE TRANSMITTED TO THE PROGENY. • TRANSGENIC TECHNOLOGY HAS LED TO THE DEVELOPMENT OF FISHES, LIVE STOCK AND OTHER ANIMALS WITH ALTERED GENETIC PROFILES WHICH ARE USEFUL TO MANKIND.
- 3. • FIRST TRANSGENIC ANIMAL WAS A ‘SUPERMOUSE’ CREATED BY RALPH BRINSTER (U PENNSYLVANIA) AND RICHARD PALMITER (UNIVERSITY OF WASHINGTON) IN 1982. • IT WAS CREATED BY INSERTING A HUMAN GROWTH HORMONE GENE IN MOUSE GENOME. • THE OFFSPRING WAS MUCH LARGER THAN THE PARENTS. • MOUSE – COMMON TRANSGENIC EXPT. • OTHER ANIMALS INCLUDE PIG, GOAT, COW, SHEEP, FISH ETC.
- 4. PRODUCTION OF TRANSGENIC ANIMALS – THE METHADOLOGY • STEP 1 – CONSTRUCTION OF A TRANSGENE • TRANSGENE MADE OF 3 PARTS: • PROMOTER • GENE TO BE EXPRESSED • TERMINATION SEQUENCE • STEP 2 – INTRODUCTION OF FOREIGN GENE INTO THE ANIMAL • PRONUCLEAR MICROINJECTION METHOD • EMBRYONIC STEM CELL METHOD.
- 5. MICROINJECTION METHOD • A FEMALE ANIMAL IS SUPER OVULATED AND EGGS ARE COLLECTED. • THE EGGS ARE FERTILIZED IN VITRO. • THE TRANSGENE CONTAINING SOLUTION IS INJECTED INTO THE MALE PRONUCLEUS USING A MICROPIPETTE. • EGGS WITH THE TRANSGENES ARE KEPT OVERNIGHT IN AN INCUBATOR TO DEVELOP TO A 2 CELL STAGE. • THE EGGS ARE THEN IMPLANTED INTO THE UTERUS OF A PSEUDO - PREGNANT FEMALE (FEMALE WHICH HAS BEEN MATED WITH A VASECTOMIZED MALE THE PREVIOUS NIGHT)
- 6. EMBRYONIC STEM CELL METHOD • TRANSGENIC ANIMALS CAN BE CREATED BY MANIPULATING EMBRYONIC STEM CELLS. • ES CELLS ARE OBTAINED FROM THE INNER CELL MASS OF A BLASTOCYST. • TRANSGENE IS INCORPORATED INTO THE ES CELL BY • MICROINJECTION • BY A RETRO VIRUS • BY ELECTROPORATION • TRANSGENIC STEM CELLS ARE GROWN IN VITRO. • THEN THEY ARE INSERTED INTO A BLASTOCYST AND IMPLANTED INTO A HOST’S UTERUS TO GROW NORMALLY.
- 8. PROBLEMS • MULTIPLE INSERTION – TOO MUCH PROTEINS • INSERTION INTO AN ESSENTIAL GENE – LETHALITY • INSERTION INTO A GENE LEADING TO GENE SILENCING • INSERTION INTO A DIFFERENT AREA CAN AFFECT THE GENE REGULATION
- 10. TRANSGENIC FISH • SUPERFISH • INCREASED GROWTH AND SIZE • GROWTH HORMONE GENE INSERTED INTO FERTILIZED EGG. • TRANSGENIC SALMON GROWS ABOUT 10 – 11 TIMES FASTER THAN NORMAL FISH. • GLO FISH • GM FRESHWATER ZEBRA FISH PRODUCE BY INTEGRATING A FLUORESCENT PROTEIN GENE FROM JELLY FISH INTO EMBRYO OF FISH.
- 11. TRANSGENIC LIVESTOCK • BIOREACTORS WHOSE CELLS HAVE BEEN ENGINEERED TO SYNTHESIS MARKETABLE PROTEINS. • MORE ECONOMICAL THAN PRODUCING DESIRED PROTEIN IN CELL CULTURE. • TRANSGENIC CATTLE • TRANSGENIC COWS ARE MADE TO PRODUCE PROTEINS LACTOFERRIN AND INTERFERONS IN THEIR MILK. • PRION FREE COWS RESISTANT TO MAD COW DISEASE. • TRANSGENIC SHEEP • FOR GOOD QUALITY WOOL PRODUCTION. • TRANSGENIC GOAT • GOATS THAT COULD EXPRESS TISSUE PLASMINOGEN ACTIVATOR, ANTI THROMBIN III, SPIDER SILK ETC IN MILK.
- 12. TRANSGENIC RABBIT • ALBA, THE EGFP (ENHANCED GREEN FLUORESCENT PROTEIN) BUNNY • CREATED IN 2000 AS A TRANSGENIC ARTWORK. • ANDI WAS THE FIRST TRANSGENIC MONKEY, BORN IN 2000. • “ANDI” STANDS FOR “INSERTED DNA” SPELLED BACKWARDS. • AN ENGINEERED VIRUS WAS USED TO INSERT THE HARMLESS GENE FOR GREEN FLUORESCENCE PROTEIN (GFP) INTO ANDI’S RHESUS GENOME. • ANDI PROVES THAT TRANSGENIC PRIMATES CAN BE CREATED, AND CAN EXPRESS A FOREIGN GENE DELIVERED INTO THEIR GENOME.
- 13. IMPORTANCE OF TRANSGENIC ANIMALS • MEDICAL IMPORTANCE • DISEASE MODEL • BIOREACTORS FOR PHARMACEUTICALS • XENOTRANSPLANTATION • AGRICULTURAL IMPORTANCE • DISEASE RESISTANT ANIMALS • FOR IMPROVING QUALITY AND QUANTITY OF MILK, MEAT, EGGS AND WOOL PRODUCTION • INDUSTRIAL IMPORTANCE • TOXICITY SENSITIVE TRANSGENIC ANIMALS TO TEST CHEMICALS. • SPIDER SILK IN MILK OF GOAT
- 14. ISSUES RELATED TO TRANSGENIC TECHNOLOGY • BLURRING THE LINES BETWEEN SPECIES BY CREATING TRANSGENIC COMBINATIONS. • THERE MAY BE HEALTH RISKS ASSOCIATED WITH TRANSGENICS. • THERE MAY BE LONG TERM EFFECTS ON THE ENVIRONMENT WHEN TRANSGENIC ANIMALS ARE RELEASED INTO THE FIELD. • VARIOUS BIOETHICIST ARGUE THAT IT IS WRONG TO CREATE ANIMALS THAT WOULD SUFFER AS A RESULT OF GENETIC ALTERATION.
- 15. CONCLUSION • TRANSGENIC TECHNOLOGY IS A FIELD THAT IS UNDER CONSTANT EVOLUTION. • MANY TRANSGENIC ANIMALS HAVE BEEN SUCCESSFULLY CREATED FOR A VARIETY OF PURPOSES, AND THE PROSPECTS ARE ENORMOUS. • IT HOLDS GREAT POTENTIAL IN MANY FIELDS INCLUDING AGRICULTURE, MEDICINE AND INDUSTRY. • WITH PROPER RESEARCH AND CAREFUL USE THE TRANSGENIC ANIMALS CAN GO A LONG WAY IN SOLVING SEVERAL PROBLEMS FOR WHICH SCIENCE DOESN’T HAVE A SOLUTION TILL NOW.