This document discusses the production of transgenic farm animals. It begins by introducing transgenic technology which involves introducing foreign DNA into an animal using recombinant DNA methods. It then describes extracting the desired gene using PCR and discusses several methods for creating transgenic animals, including retroviral vectors, sperm-mediated gene transfer, microinjection, and embryonic stem cells. Potential applications are improved biomass, disease resistance, recombinant vaccines, and livestock pharming. Examples of transgenic animals produced include sheep, goats, pigs, cattle, fish, and chickens.

1. PRODUCTION OF
TRANSGENIC FARM
ANIMALS
Prepared by: MUHSIN ALI AHMED
M.Sc. in Biotechnology
from Bangalore University-India
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2. CONTENTS:
Introduction
Extraction of Desire Gene
Methods of Transgenic Animal
Application of Animal Transgenic
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3. INTRODUCTION
 Foreign DNA(gene) is introduced into the animal,
using recombinant DNA technology.
 Deoxyribonucleic acid (DNA) is unit of genetic
which is responsible of all quality and quantity
animal characters.
 DNA coiled and coiled with histone protein to
produce chromosome .
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4. EXTRACTION DESIRE GENE
 For extraction of specific gene from of millions of
gene we need to Polymerase Chain Reaction
(PCR) technique and include:
 DNA extraction
 Thermo cycle device
 Gene primer
 Some chemical materials
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5. METHODS OF TRANSGENIC ANIMAL
1- Retroviral Vector Method
2- Sperm Mediated Gene Transfer
3- Microinjection Method
4- Embryonic Stem Cell
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6. 1- RETROVIRAL VECTOR METHOD
 when the virus vectors infect a host cell, the viral
RNA is reverse transcribed in the cytoplasm making
linear double-stranded DNA.
 A retrovirus is a virus that carries its genetic
material in the form of RNA rather than DNA.
 In this method, retroviruses are used as vectors to
transfer genetic material into the host cell
 Infecting mouse embryo with a retrovirus which
carry the new gene. 6

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8. 2- SPERM MEDIATED GENE TRANSFER
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9. 3- MICROINJECTION METHOD
 1) transfer of a desired gene construct (of a single
gene or a combination of genes that are
recombined and then cloned) from another member
of the same species or from a different species into
the pronucleus of a reproductive cell;
 2) in vitro culture of the manipulated cells to
develop to a specific embryonic phase; and
 3) then transfer of the embryonic cells to the
recipient female.
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11. 4- EMBRYONIC STEM CELL
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13. APPLICATION OF ANIMAL TRANSGENIC
1- Agriculture like improve Biomass
2- Disease Resistance like avian influenza.
3- Recombinant Vaccines for poultry
4- Livestock Pharming – Products like
myelin production
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14. ANIMAL PRODUCTED FROM
TRANSGENIC
1- Transgenic Sheep
2- Transgenic Goat
3- Transgenic Pigs
4- Transgenic Cattle
5- Transgenic Fish
6- Transgenic Chicken
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15. REFERENCES
 Robl JM, Wang Z, Kasinathan P, Kuroiwa Y (2007)
Transgenic animal production and animal
biotechnology. Theriogenology 67:127–133
 Gorman, C. (1986). ‘High efficientcy gene transfer
into mammalian cells’, In: DNA cloning Vol. II,
apractical approach, ed., E.M. Glover., IRL Press,
Oxford
 Jogdand, S.N. (2003). ‘Gene Biotechnology’,
Second reversed Edition, Himalaya Publishing
House.
 Gordon, J.W. Ruddle, F.H.( 1983). ‘Gene trancfer
into mouse embryo’, first edition, Kalyani Publishing
House.
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16. TANK YOU
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