1. A transgenic animal is one that has had a foreign gene deliberately inserted into its genome. The first transgenic animal was a "Supermouse" created in 1982 by inserting a human growth hormone gene.
2. There are three main steps to creating a transgenic animal: construction of the transgene, introduction of the gene into the animal, and screening progeny for integration of the gene. Methods like pronuclear microinjection and embryonic stem cell manipulation are used.
3. Transgenic animals have applications in medicine, agriculture, and industry. They are used as disease models, to produce pharmaceuticals, for improved food production, and to test chemicals.
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
Transgenesis is the future of healthcare where the world is focusing on it so why not us? Let's delve into the exclusive depth of this transgenesis in the slide.
Refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation.
Foreign DNA is introduced into the animal, using recombinant DNA technology,
it contain some production techniques of transgenic animals with some examples and utility in drug development (available transgenic animals model of drug and their activity).
Applications and uses in different field
Another techniques like transposons and knock-out & knock-in discussed later
Transgenesis is the future of healthcare where the world is focusing on it so why not us? Let's delve into the exclusive depth of this transgenesis in the slide.
Refers to an animal in which there has been a deliberate modification of the genome - the material responsible for inherited characteristics - in contrast to spontaneous mutation.
Foreign DNA is introduced into the animal, using recombinant DNA technology,
Transgenic animal production and its applicationkishoreGupta17
A genetically modified animal with the heterologous gene of interest being inserted for the purpose of biopharming or make a diseased model to study the consequences of disease and its probable therapy
This is about methods of creating transgenic animals,applications of transgenic animals in biotechnology and application of transgenic animals in pharmaceuticals.
A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.
Transgenesis is the process by which mixing up of genes takes place.
Foreign genes are inserted into the germ line of the animal, so it can be transmitted to the progeny.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.
First transgenic animal was a ‘Supermouse’ created by Ralph Brinster (U Pennsylvania) and Richard Palmiter (University of Washington) in 1982.
It was created by inserting a human growth hormone gene in mouse genome.
The offspring was much larger than the parents.
Mouse – common transgenic expt.
Other animals include pig, goat, cow, sheep, fish etc.
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
Transgenic animal production and its applicationkishoreGupta17
A genetically modified animal with the heterologous gene of interest being inserted for the purpose of biopharming or make a diseased model to study the consequences of disease and its probable therapy
This is about methods of creating transgenic animals,applications of transgenic animals in biotechnology and application of transgenic animals in pharmaceuticals.
A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.
Transgenesis is the process by which mixing up of genes takes place.
Foreign genes are inserted into the germ line of the animal, so it can be transmitted to the progeny.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.
First transgenic animal was a ‘Supermouse’ created by Ralph Brinster (U Pennsylvania) and Richard Palmiter (University of Washington) in 1982.
It was created by inserting a human growth hormone gene in mouse genome.
The offspring was much larger than the parents.
Mouse – common transgenic expt.
Other animals include pig, goat, cow, sheep, fish etc.
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
It's include all the details about the transgenic technology.all the techniques like micro injection,SCNT,pro nuclear injection method.It include all the Transgenic mice bird and fish.
transgenic animals , its production and applicationMonishaKCReddy
Process of introducing a foreign or exogenous DNA into an animal genome is called as Transgenesis
Transgenesis is the process of introducing an exogenous gene called a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
Retroviruses used as vectors to transfer genetic material into the host cell
Retroviruses can be used for the transfer of foreign genes into animal genomes.
Embryonic stem cell-mediated gene transfer.
Involves prior insertion of the desired DNA sequence by homologous recombination into an in vitro culture of embryonic stem (ES) cells. Incorporated into an embryo at the blastocyst stage of development.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
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and write to us if you have any questions:
info@kuddlelife.org
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Epcon is One of the World's leading Manufacturing Companies.EpconLP
Epcon is One of the World's leading Manufacturing Companies. With over 4000 installations worldwide, EPCON has been pioneering new techniques since 1977 that have become industry standards now. Founded in 1977, Epcon has grown from a one-man operation to a global leader in developing and manufacturing innovative air pollution control technology and industrial heating equipment.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Climate Change All over the World .pptxsairaanwer024
Climate change refers to significant and lasting changes in the average weather patterns over periods ranging from decades to millions of years. It encompasses both global warming driven by human emissions of greenhouse gases and the resulting large-scale shifts in weather patterns. While climate change is a natural phenomenon, human activities, particularly since the Industrial Revolution, have accelerated its pace and intensity
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Transgenic animals
1. ADVANCE COLLEGE OF PHARMACY
Kanpur
DEPARTMENT OF PHARMACOLOGY
Mr. ANUBHAV DUBEY
M.Pharma
pharmacology
TRANSGENIC ANIMALS
Presented- By
2. A transgenic animal is one that carries a foreign gene
that has been deliberately inserted into its genome.
Transgenesis is the process by which mixing up of genes
takes place.
Foreign genes are inserted into the germ line of the
animal, so it can be transmitted to the progeny.
Transgenic technology has led to the development of
fishes, live stock and other animals with altered genetic
profiles which are useful to mankind.
3. First transgenic animal was a ‘Supermouse’ created by Ralph
Brinster (U Pennsylvania) and Richard Palmiter (University of
Washington) in 1982.
It was created by inserting a human growth hormone gene in
mouse genome.
The offspring was much larger than the parents.
Mouse – common transgenic(9%-90%)
Other animals include pig, goat, cow, sheep, fish etc. (5%-10)
4. Step 1 – Construction of a transgene
Transgene made of 3 parts:
Pomoter
Gene to be expressed
Termination sequence
Step 2 – Introduction of foreign gene into the animal
Pronuclear microinjection method
Embryonic stem cell method.
5. A female animal is superovulated and eggs are collected.
The eggs are fertilized in vitro.
The transgene containing solution
is injected into the male pronucleus
using a micropipette.
Eggs with the transgenes are kept overnight in an
incubator to develop to a 2 cell stage.
The eggs are then implanted into the uterus of a pseudo -
pregnant female (female which has been mated with a
vasectimized male the previous night)
6. Transgenic animals can be created by manipulating
embryonic stem cells.
ES cells are obtained from the inner cell mass of a blastocyst.
Transgene is incorporated into the ES cell by
Microinjection
By a retro virus
By electroporation
Transgenic stem cells are grown in vitro.
Then they are inserted into a blastocyst and implanted into a
host’s uterus to grow normally.
7.
8. Step 3: Screening for transgenic positives
Transgenic progenies are screened by PCR to examine the
site of incorporation of the gene
Some transgenes may not be expressed if integrated into a
transcriptionally inactive site.
Step 4: Further animal breeding is done to obtain
maximal expression.
Heterozygous offsprings are mated to form homozygous
strains.
9.
10.
11. Superfish
Increased growth and size
Growth hormone gene inserted
into fertilized egg.
Transgenic salmon grows about
10 – 11 times faster than
normal fish.
Glo fish
GM freshwater zebra fish
(Danio rerio)
Produce by integrating a
fluorescent protein gene from
jelly fish into embryo of fish.
12. Alzheimer’s mouse
In the brain of Alzheimer’s patients, dead
nerve cells are entangled in a protein
called amyloid.
Mouse made by introducing amyloid
precursor gene into fertilized egg of mice.
Oncomouse
Mouse model to study cancer
Made by inserting activated oncogenes.
Smart mouse
Biological model engineered to overexpress NR2B receptor in
the synaptic pathway.
This makes the mice learn faster like juveniles throughout their
lives.
13. Enviro pig
Pigs have trouble fully digesting a
compound known as phytate found in many cereal grains
used to feed them.
Transgenic pigs are created by introducing phytase gene
of E.coli.
Phytase enzyme is thus produced in the salvary gland of pig
It degrades indigestible phytate with the release of
phosphate that is readily digested by pigs.
Pig for organ transplant
Pigs with human genes, in order to decrease the chance
of organ rejection by human body.
14. Bioreactors whose cells have been engineered to synthesis
marketable proteins.
More economical than producing desired protein in cell
culture.
Transgenic cattle
Transgenic cows are made to produce proteins lactoferrin
and interferons in their milk.
Prion free cows resistant to mad cow disease.
Transgenic sheep
For good quality wool production.
Transgenic goat
Goats that could express tissue plasminogen
activator, anti thrombin III, spider silk etc in milk.
15. Medical importance
Disease model
Bioreactors for pharmaceuticals
Xenotransplantation
Agricultural importance
Disease resistant animals
For improving quality and quantity of milk, meat, eggs and
wool production
Industrial importance
Toxicity sensitive transgenic animals to test chemicals.
Spider silk in milk of goat
16. Alba, the EGFP (Enhanced Green Flurescent
protein) bunny
Created in 2000 as a transgenic artwork.
ANDi was the first transgenic monkey, born in
2000.
“ANDi” stands for “inserted DNA” spelled
backwards.
An engineered virus was used to insert the
harmless gene for green fluorescence protein
(GFP) into ANDi’s rhesus genome.
ANDi proves that transgenic primates can be
created, and can express a foreign gene delivered
into their genome.
17. Blurring the lines between species by creating transgenic
combinations.
There may be health risks associated with transgenics.
There may be long term effects on the environment when
transgenic animals are released into the field.
Various bioethicist argue that it is wrong to create animals
that would suffer as a result of genetic alteration.
18. Transgenic goats engineered to produce human breast
milk ( Journal Transgenic Research, August 2012)
University of California scientists created the transgenic goats
by transferring human genes for breast milk enzymes and
proteins into goat embryos.
Produce 60 percent of the lysozyme and lactoferrin found in
human mother’s milk.
For babies of mothers who aren’t present, or can’t nurse
them, milk from these transgenic goats could provide the next-
best alternative.
Bioluminescent transgenic mouse model for study
mammary gland tumour development. (PLoS ONE, aug 2012)
Research done by Cancer Biology and Therapeutics
Group, Ireland
19. Transgenic technology is a field that is under constant
evolution.
Many transgenic animals have been successfully created for a
variety of purposes, and the prospects are enormous.
It holds great potential in many fields including agriculture,
medicine and industry.
With proper research and careful use the transgenic animals
can go a long way in solving several problems for which
science doesn’t have a solution till now.