The document outlines a syllabus for a virology and mycology course, detailing six units covering mycology, fungal infections, virology, RNA and DNA viruses, and PCR techniques. It includes key topics such as the classification of viruses, the pathology of viral diseases, diagnostic methods, and treatment approaches. The course aims to provide comprehensive knowledge of medically important fungi and viruses, including their characteristics, transmission, and laboratory diagnosis.

1. Module- 4
Topic name
RHINO VIRUS NOTES
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo
viruses, Measles Mumps, Rubella, HIV
Unit 4

4. NOTES

5. Classification / Families of viruses
DNA Viruses RNA Viruses
HAP3
Herpes viridae
1. Alpha – HS1, HS2, EBV
2. Beta – CMV
3. Gama -VZ
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
PR2OCBT
H H
PCR2MMR3 – IH
1. Polio virus
2. Corona virus
3. Rhino virus
4. Rota virus
5. Measles virus
6. Mumps virus
7. Rubella
8. Rabis
9. Influenza
10. HIV
AIDS

6. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

7. 1. Introduction
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

8. Other viruses that cause common cold
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

9. 2. Classification1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

10. 3. Morphology
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10. T

11. 4. Susceptibility & Resistance
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10. T

12. 5. Cultivation

13. 6. Pathogenesis

14. 7. Symptoms of common cold

15. 8. Laboratory Diagnosis