This course is designed to provide Medical Lab Technology students with a comprehensive understanding of the medical microbiology laboratory's operation. It starts with foundational knowledge in laboratory safety, equipment, and microbial classification and then advances to diagnostic microbiology techniques, immunology, and serology. Lastly, it explores more sophisticated lab procedures such as molecular diagnostics, virology, and recent trends in the field. Each unit comes with real-life case studies to further reinforce the application of theoretical knowledge. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Clinical Laboratory Visit - V
Course Name: Clinical Laboratory Visit - V Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Skill
Enhancement
PRE-REQUISITE Clinical Laboratory Visit - IV Sem: 6 S. No: 46
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course is designed to provide Medical Lab Technology students with a comprehensive
understanding of the medical microbiology laboratory's operation. It starts with foundational
knowledge in laboratory safety, equipment, and microbial classification and then advances to
diagnostic microbiology techniques, immunology, and serology. Lastly, it explores more
sophisticated lab procedures such as molecular diagnostics, virology, and recent trends in the
field. Each unit comes with real-life case studies to further reinforce the application of
theoretical knowledge.
B. COURSE OBJECTIVES:
1. Recall the core functions of a medical microbiology lab, safety protocols, and essential lab
equipment.
2. Describe the classification and characteristics of microorganisms, basic staining and culture
methods, and serology techniques.
3. Apply knowledge of microbiology to interpret case studies, diagnose infections using lab
techniques, and perform basic serology and immunohematology procedures.
4. Analyze case studies to infer the practical application of lab techniques, microbial pathogenesis,
and antimicrobial susceptibility testing.
5. Evaluate the role of advanced techniques like molecular diagnostics and genomics in disease
diagnosis and tracking. Discuss the current trends and future prospects in medical microbiology.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will acquire foundational knowledge of
medical microbiology laboratory's operation,
including understanding of safety protocols, use of
essential lab equipment, and the classification of
microorganisms.
PI 2.1, PI 4, PI
6
K1: Knowledge 70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO2 Students will comprehend the principles of basic
laboratory techniques such as staining, culture
methods, and serology. They will understand the
immune response to infection and the role of
microorganisms in health and disease.
PI 2.5, PI 6, PI
7, PI 8
K2:
Comprehension
70%
CO3 Students will be capable of applying learned
techniques to collect and handle biological samples,
perform microbial staining and culture, and conduct
basic serology and immunohematology procedures.
PI 2.2, PI 2.3,
PI 2.4, PI 2.5,
PI 7
K3: Application 70%
CO4 Students will be able to analyze microbial cultures,
interpret antimicrobial susceptibility tests, and
deduce information from molecular diagnostic
results to assist in infection diagnosis and treatment.
PI 2.7, PI 2.8,
PI 3, PI 6
K4: Analysis 70%
CO5 Students will evaluate the effectiveness of different
diagnostic methods and the role of advanced
techniques in disease diagnosis, tracking, and
control. They will also assess the importance of
antimicrobial stewardship and the One Health
concept.
PI 3, PI 4, PI 6,
PI 9
K5: Evaluation 70%
CO6 Students will develop the ability to integrate
knowledge from various areas of medical
microbiology to propose solutions for real-world
problems, such as controlling an outbreak or
improving lab protocols.
PI 1, PI 3, PI 5,
PI 9, PI 10
K6: Creation 70%
D. SYLLABUS
Unit-1 Basic Laboratory Skills and Microbial Classification Contact Hours:15
Chapter 1.1 Introduction to the Medical Microbiology Laboratory- Overview of the lab and its
functions, Safety protocols, Introduction to lab equipment, Sample collection, handling, and
processing
Chapter 1.2 Classification and Characteristics of Microorganisms- Introduction to microbiology,
Classification of microorganisms, Morphological and biochemical characteristics, Role and
importance of microorganisms in health
Experiment 1 /
Case study
Case study "Maintaining Safety Protocols: The Forgotten Glove" – This case study could
illustrate the importance of safety procedures and proper personal protective equipment
usage in the laboratory
Experiment 2 /
Case study
Case study: "The Impact of Proper Sample Handling: A Misdiagnosed Case" – A case study
showcasing how improper sample handling can lead to incorrect diagnosis.
Experiment 3 /
Case study
Case study: "The Misidentified Microbe: An Unusual Infection" – This case study would focus
on the importance of correctly identifying microbes in disease diagnosis and treatment.
Unit-2 Diagnostic Microbiology and Immunology Contact Hours:15
Chapter 2.1 Diagnostic Microbiology Techniques - 46
Chapter 2.2 Clinical Immunology and Serology- The immune response to infection, Basic serology
techniques, Immunohematology and blood banking, Autoimmune and immunodeficiency
disorders
Experiment 4 /
Case study
Case study: "From Gram Stain to Diagnosis: The Tale of a Persistent Infection" – A case study
demonstrating the application of Gram staining and culture techniques in disease diagnosis.
Experiment 5 /
Case study
Case study: "Resistance Is Futile: The Case of a Resilient Pathogen" – This case study could
explore antimicrobial resistance testing in the laboratory.
Experiment 6 /
Case study
Case study: "Unmasking the Invisible: Serology and the Stealth Virus" – This case study could
illustrate how serological testing can detect infections even when the pathogen isn't directly
identifiable.
Experiment 7 /
Case study
Case study: "Blood Type Dilemma: The Transfusion Mix-up" – A case study focusing on
blood typing and the implications of errors in blood transfusions.

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Unit-3 Advanced Laboratory Procedures and Emerging Trends Contact Hours:15
Chapter 3.1 Molecular Diagnostics and Virology- Principles of molecular diagnostics, Viral cultivation
and identification, Molecular virology, Viral pathogenesis
Chapter 3.2 Emerging Trends in Medical Microbiology- Advanced antimicrobial susceptibility testing,
Rapid diagnostics, Role of genomics in microbiology, Antimicrobial stewardship, One health
concept
Experiment 8 /
Case study
Case study: "From DNA to Diagnosis: Detecting a Rare Viral Infection" – A case study
emphasizing the role of molecular diagnostics in detecting uncommon or challenging viral
diseases.
Experiment 9 /
Case study
Case study: "The Outbreak Traceback: Using Virology in Epidemiology" – This case study
could examine how virology techniques help trace and understand disease outbreaks.
Experiment 10 /
Case study
Case study: "Predicting Pathogens: The Role of Genomics in Tracking a Hospital Outbreak"
– A case study highlighting how genomics can help identify, track, and contain a pathogen
outbreak in a hospital setting.
Self-study topics for Advance learners:
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Forbes, B. A., Sahm, D. F., & Weissfeld, A. S. (2007). Bailey & Scott's Diagnostic
Microbiology. Mosby.
T2 Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2015). Medical Microbiology. Elsevier.
T3 Mahon, C. R., Lehman, D. C., & Manuselis, G. (2018). Textbook of Diagnostic
Microbiology. Elsevier Health Sciences.
REFERENCE BOOKS
R1 Willey, J., Sherwood, L., & Woolverton, C. (2016). Prescott's Microbiology. McGraw-
Hill.
R2 Koneman, E. W. (2006). Koneman's Color Atlas and Textbook of Diagnostic
Microbiology. Lippincott Williams & Wilkins.
R3 Jorgensen, J. H., Pfaller, M. A., & Carroll, K. C. (2015). Manual of Clinical Microbiology.
ASM Press.

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.