This course aims to provide students with an in-depth understanding of blood banking, including the concepts of blood grouping, compatibility testing for transfusion, and the management of blood resources. It also delves into the fundamental principles of genetics, focusing on inheritance patterns, chromosomal basis of inheritance, and the role of DNA and RNA in protein synthesis. Through practical exercises, students will gain hands-on experience on various techniques used in blood banking and genetics. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Blood Bank and Genetics
Course Name: Blood Bank and Genetics Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Advanced Haematology, Applied Clinical
Biochemistry – II
Sem: 7 S. No: 48
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course aims to provide students with an in-depth understanding of blood banking,
including the concepts of blood grouping, compatibility testing for transfusion, and the
management of blood resources. It also delves into the fundamental principles of genetics,
focusing on inheritance patterns, chromosomal basis of inheritance, and the role of DNA and
RNA in protein synthesis. Through practical exercises, students will gain hands-on experience
on various techniques used in blood banking and genetics.
B. COURSE OBJECTIVES:
1. Identify the historical milestones and significant discoveries related to various blood group
systems.
2. Explain the concepts of blood grouping, compatibility testing, and the potential sources of error in
blood grouping.
3. Conduct various experiments related to blood banking, such as blood grouping, compatibility
testing, and the preparation of various blood fractions for transfusion.
4. Analyze the chromosomal basis of inheritance and the role of DNA and RNA in protein synthesis.
5. Create a comprehensive understanding of human genetics, incorporating concepts from molecular
genetics and microbial genetics.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Recall the basic concepts and principles of blood
banking, blood group systems, and the primary
elements of genetics.
PI 2.5, PI 6 Knowledge (K1) 70%
CO2 Understand the implications of blood compatibility
testing and the potential complications and hazards
of blood transfusion.
PI 2, PI 3 Comprehension
(K2)
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO3 Apply knowledge of blood banking techniques and
procedures in real-world scenarios such as blood
grouping, compatibility testing, and preparation of
various blood fractions for transfusion.
PI 2.2, PI 2.4,
PI 2.6, PI 7
Application
(K3)
70%
CO4 Analyze the genetic patterns of inheritance,
chromosomal structure, and the role of DNA and
RNA in protein synthesis.
PI 2.1, PI 2.5,
PI 3, PI 6
Analysis (K4) 70%
CO5 Combine knowledge of molecular genetics, human
genetics, and microbial genetics to form a holistic
understanding of the field.
PI 2.5, PI 6, PI
8
Synthesis (K5) 70%
CO6 Critically evaluate the quality control measures in
blood banks, including donor selection, blood
collection and storage, and the handling of blood
resources.
PI 2.3, PI 2.7,
PI 2.9, PI 4
Evaluation (K6) 70%
D. SYLLABUS
Unit-1 Basic Principles of Blood Banking Contact Hours:15
Chapter 1.1 Introduction to Blood Banking and Blood Group Systems- Introduction to Blood Banking,
History and discovery of various blood group systems, ABO blood group system, Rh and other
major blood group system
Chapter 1.2 Blood Grouping and Compatibility Testing- Sources of error in blood grouping and their
elimination, ABO grouping: Forward and reverse grouping, Causes of discrimination between
forward and reverse grouping, Rh grouping, Compatibility test in blood transfusion, Use of
enzymes in blood bank specially Papain
Experiment 1 /
Case study
Introduction to Blood Banking practical operations
Experiment 2 /
Case study
ABO and Rh blood grouping
Experiment 3 /
Case study
Compatibility testing for blood transfusion
Unit-2 Blood Transfusion and Quality Control in Blood Banks Contact Hours:15
Chapter 2.1 Transfusion Reactions and Investigations - 48
Chapter 2.2 Blood Collection, Storage and Quality Controlq- Various anticoagulants used to collect
blood for transfusion purposes, Selection of donor and procedure for collection of blood from
a healthy donor, Preparation of various fractions of blood for transfusion and therapeutic
purposes, Brief introduction of blood substitute/artificial blood, Haemopheresis: pertaining to
Leucocytes, platelets and plasma, Quality control in blood bank
Experiment 4 /
Case study
Identification of transfusion reactions and mismatched blood transfusion
Experiment 5 /
Case study
Preparation of different fractions of blood for transfusion
Experiment 6 /
Case study
Blood donation simulation and collection
Experiment 7 /
Case study
Quality control measures in blood banks
Unit-3 Introduction to Genetics Contact Hours:15
Chapter 3.1 Fundamental Principles of Genetics- Continuity of life-heredity, variation, Mendel's laws of
inheritance, Chromosomal basis of inheritance, Other patterns of inheritance- incomplete
dominance, multi parallelism, quantitative inheritance
Chapter 3.2 Molecular Genetics and Human Genetics- Chromosomes - bacterial cell and eukaryotic cell,
Parallelism between genes and chromosomes, Genome, Linkage and crossing over, Gene
mapping, Recombination, Molecular genetics: DNA as a genetic material- its structure and

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
replication, Structure of RNA and its role in protein synthesis, Vectors, plasmids, Human
Genetics, Microbial genetics
Experiment 8 /
Case study
Mendelian inheritance patterns and laws
Experiment 9 /
Case study
Chromosomal studies in eukaryotic cells
Experiment 10 /
Case study
DNA structure and replication experiment
Self-study topics for Advance learners: Transfusion Medicine in Special Populations:
Understanding the specific considerations for blood transfusions in neonates, geriatrics,
immunocompromised patients, and patients with rare blood types.,Management and Administration in
Blood Banking: This topic would cover blood bank regulations, accreditation, quality control, and
inventory management, along with other administrative aspects of running a blood bank., Transfusion
Reactions and Complications: Delve deeper into the immunological reactions, hemolytic disorders,
and infectious complications associated with blood transfusion., Hemovigilance and Patient Blood
Management: This topic would cover surveillance procedures for collecting and assessing information
on unexpected or adverse effects of blood transfusion, with a focus on patient safety and
outcomes.Advanced Immunohematology: This would include the study of rare blood groups, antibody
identification and titration, and the role of genomics in blood typing.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Ross and Wilson Anatomy and Physiology in Health and Illness by Anne Waugh and
Allison Grant
T2 Blood Cells: A Practical Guide by Barbara J. Bain
T3 Modern Blood Banking & Transfusion Practices by Denise M. Harmening
REFERENCE BOOKS
R1 Human Molecular Genetics by Tom Strachan and Andrew Read
R2 Molecular Genetics of Bacteria by Larry Snyder, Joseph E. Peters, Tina M. Henkin, Wendy
Champness
R3 Emery's Elements of Medical Genetics by Peter D Turnpenny, Sian Ellard

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.