The document outlines a syllabus for a course on virology and mycology, including topics such as medical mycology, fungal infections, virology, RNA and DNA viruses, and next-generation PCR applications. It covers essential aspects of virus classification, pathogenesis, laboratory diagnosis, and treatment options. Additionally, it includes specific details about arboviruses and febrile illnesses, emphasizing their causes and related conditions.

1. Module- 4
Topic name
ARBO VIRUSES
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo
viruses, Measles Mumps, Rubella, HIV
Unit 4

4. Classification / Families of viruses
RNA Viruses
PR2OCBT
H
PCR2MMR3 – IH
1. Polio virus
2. Corona virus
3. Rhino virus
4. Rota virus
5. Measles virus
6. Mumps virus
7. Rubella
8. Rabis
9. Influenza
10. HIV
AIDS

5. NOTES

6. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

7. INTRODUCTION

8. Arthropod Born Viruses

23. NOTES

24. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

25. INTRODUCTION

26. Introduction

27. Introduction to Arboviruses

28. Classification of Arbo viruses
TFBRO
2

35. All these viruses cause any one of these
diseases
1. Encephalitis 2. Febrile illness 3. Hemorrhagic
fever

38. Febrile illness definition
• Febrile Illness with Uncertain Cause (Adult)
• You have a fever, but the cause is unknown. A fever is the
body's natural reaction to an illness such as infection due to a
virus or bacteria. Sometimes other conditions such as cancer
or immune diseases can cause fever. This might be more likely
if your fever has lasted for more than a week or 2. In most
cases, the higher temperature itself isn't harmful. It actually
helps the body fight infections. A fever doesn't need to be
treated unless you feel very uncomfortable.
• Sometimes a fever can be an early sign of a more serious
infection. So follow up if your condition gets worse.

42. Dengue

43. Introduction

50. Objective questions

51. MCQ’s

52. Answers