This document compares differential leukocyte counts obtained from peripheral blood smears to those from an automated cell counter. Ten blood samples were analyzed using both methods. The results showed little difference between the two methods. This suggests that automated cell counters provide accurate readings and can be preferred over manual counts from smears to save time. Factors that could cause errors in differential counts include improper sample collection, staining issues, and calculation mistakes. Taking proper precautions in sample collection and analysis can help avoid errors.
An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils.
Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
An immature red blood cell without a nucleus, having a granular or reticulated appearance when suitably stained.
Reticulocytes are the immature RBC that contain nucleus.
They are originally seen at the site of their formation i.e. bone marrow. They take 2-3 (lays for maturation only about 1-2% of circulating RBCs are Reticulocytes.
Differential count is the % distribution of various white cells in the peripheral blood. It is determined from a blood smear stained with a polychromatic stain & alters examination of the stained smear by using oil-immersion objective. The stained blood smear also helps to study abnormal morphology of leucocytes & red cells. Study of blood smear helps in the diagnosis of various anaemia's, leukaemia & detection of blood parasites.
An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils.
Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
An immature red blood cell without a nucleus, having a granular or reticulated appearance when suitably stained.
Reticulocytes are the immature RBC that contain nucleus.
They are originally seen at the site of their formation i.e. bone marrow. They take 2-3 (lays for maturation only about 1-2% of circulating RBCs are Reticulocytes.
Differential count is the % distribution of various white cells in the peripheral blood. It is determined from a blood smear stained with a polychromatic stain & alters examination of the stained smear by using oil-immersion objective. The stained blood smear also helps to study abnormal morphology of leucocytes & red cells. Study of blood smear helps in the diagnosis of various anaemia's, leukaemia & detection of blood parasites.
Blood smear preparation, fixation, staining. Veterinary Clinical Examination ...Gansbaai SA
How to make a blood smear, including all steps: Preparation, fixation and staining. Veterinary clinical Examination for small and large animals. Methanol - Fixation. Additionaly basic information about blood.
A blood smear is a sample of blood that's spread on a glass slide which is treated with a special stain. In the past, all blood smears were examined under a microscope by laboratory professionals. Now automated digital systems may be used to help examine blood smears.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
The Gram stain is a fundamental technique in microbiology used to classify bacteria based on their cell wall structure. It provides a quick and simple method to distinguish between Gram-positive and Gram-negative bacteria, which have different susceptibilities to antibiotics
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Best Ayurvedic medicine for Gas and IndigestionSwastikAyurveda
Here is the updated list of Top Best Ayurvedic medicine for Gas and Indigestion and those are Gas-O-Go Syp for Dyspepsia | Lavizyme Syrup for Acidity | Yumzyme Hepatoprotective Capsules etc
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
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Dlc by peripheral smear comparison with automated differential
1. DLC BY PERIPHERAL SMEAR
COMPARISION WITH AUTOMATED
DIFFERENTIAL COUNT
Dr Nikhil Bansal
J.N.M.C.,Wardha
2. ACKNOWLEGMENT
Skill based project is an art to enhance the creativity
of a student and to increase his/her interest in clinical
basis.
As per student manner I firstly would like to thank
“Dr. S. Vagha Ma’am” for arranging such project. I
also thank “Dr Samarth Shukla Sir” for helping us
whenever required. My pay my heartful gratitude to
“Dr.Vijay Kumar Dembra Sir” for guiding us
throughout the project.
Last but not the least I thank all my “group mates”
for co-operating with me and lending a helpful hand
when required.
3. INTRODUCTION
WBC or leukocyte is the colourless and
nucleated formed element of blood. Leukocyte
play very important role in defense mechanism of
body.
Leukocyte are classified into 2 types namely –
1. Granulocyte-with granules
2. Agranulocyte- without granules
The granulocyte are-Neutrophills
Eosinophills
Basophills
The agranulocyte are-Monocytes
Lymphocytes
4. MORPHOLOGY
Neutrophils-neutrophils have fine or small granules in
the cytoplasm granules appear violet in colour. The
nucleus is multilobed.nucleus has 4-5 lobes.
diameter – 10-12 microns
Eosinophils-eosinophils have larger granules in the
cytoplasm which stain bright red colour.the nucleus is
bilobed.
diameter-10 and 14 microns
Basophils-basophils also have coarse granules in the
cytoplasm.the granules stain purple blue with basic dyes
like methylene blue.
diameter-8 to 10 microns
5. Monocytes-monocytes are largest leucocytes with
diameter of 14 to 18 microns.the cytoplasm is clear
without granules.the nucleus is
kidney,round,oval,horseshoe shaped.
Lymphocytes-the lymphocyte also have clear cytoplasm
without granules.the nucleus is ovel shaped occupying
the whole of the cytoplasm.depending upon the size the
lymphocyte are divided into two group as:
large lymphocytes-the younger cell with a diameter of
10 to 12 microns.
Small lymphocytes-the older cell with a diameter of 7
to 10 microns
Depending upon the function,the lymphocytes are divided
into 2 types as:
T lymphocytes-concerned with cellular immunity
B lymphocytes-concerned with humoral immunity
6.
7. NORMAL COUNT OF WBC
Total WBC count – 4,000 to 11,000/cu.mm of
blood.
Differential WBC count:
LEUCKOCYTE PERCENTAGE ABSOLUTE
VALUE
Neutrophils 50 to 70 % 3000-6000
Lymphocytes 20 to 30 % 1500-2700
Eosinophils 2 to 4 % 150-450
Monocytes 2 to 6 % 200-600
Basophils 0 to 1 % 0-100
8. CONDITION IN WHICH
ALTERATION IN DLC
Neutrophilia-increased in neutrophil
count called neutrophilic leukocytosis.this
occurs in the following condition:
1) Acute infections
2) Metabolic disorders
3) Injection of vaccines
4) After acute hemorrhage
9. Lymphocytosis - increasd in lymphocyte
count is called lymphocytosis and this
occurs in
1) Diptheria
2) Infectious hepatitis
3) Mumps
4) Malnutrition
5) Rickets
6) Syphilis
10. Eosinophilia-increasd in eosinophil count
is called eosinophilia and this occurs in
1) Allergic condition
2) Asthma
3) Blood parasitism
4) Scarlet fever
13. AIM & OBJECTIVES
“ To determine the Differential leukocyte
count of the given sample in peripheral
smear and compare it with the
automated cell counter ’’
To compare differential leukocyte count
in peripheral smear with automated
cell counter.
To check the accuracy of automated cell
counter.
16. METHODS:
1) Slide method
-place a drop of blood in the centre of a clean glass
slide 1 to 2 cm from one end.
-place another slide with smooth edge at an angle
of 30-45 0 near the drop of blood.
-move the spreader backward so that it makes
contact with drop of blood.
-then move the spreader forward rapidly over the
slide.
-a thin peripheral blood film is thus prepared.
-Dry it and stain.
17.
18. Qualities of a good blood film-
1) It should not cover the entire surface of
slide.
2) It should have smooth and even appearance.
3) It should be free from waves and holes.
4) It should not have irregular tail.
Parts of a thin blood film-consists of 3 parts
1) Head-i.e. the portion of blood film near the
drop of blood.
2) Body-i.e. the main part of blood film.
3) Tail-i.e. the tapering end of the blood film.
19. 2) cover glass method-
-Take a no.1 (22 mm square)clean cover slide.
-Touch it on to the drop of blood.
-place it on another similar cover glass in cross wise direction
with slide containing drop of blood facing down.
-pull the cover glass quickly.
-Dry it and stain.
3) spine method-
This is an automated method
-place a drop of blood in the center of a glass slide.
-spin at a high speed in a special centrifuge cytospin.
-blood spreads uniformly.
-dry it and stain it.
20. PROCEDURE FOR STAINING
-pour leishman’s stain drop wise on the slide and wait
for 2 min.this allows fixation of the PBF in methyl
alcohol.
-add double the quantity of buffered water drop wise
over the slide.
-mix by rocking for 8 mints.
-wash it water for 1 to 2 mints.
-dry in air and examine under oil immersion lens of the
microscope.
21. COMPONENT OF LEISHMEN’S
STAIN
-preparation dissolve 0.2g of powdered
leishmens dye in 100 ml of acetone free
methyl alcohol in a conical flask.
-warm it to 50˚c for half an hour with
occasional shaking.
-cool it and filter it.
22. OBSERVATION
NAME A/S MRD
CELL COUNTER(%) PEREPHERAL SMEAR (%)
NO.
LYMP GRA MID P L E M B
H
1 SAINA 4/F 987808 21.0 74.7 4.3 75 22 02 01 00
2 KAVITA 22/F 907818 40.0 54.6 5.4 55 40 03 02 00
3 SUMAN 60/F 789149 21.6 74.3 4.1 76 20 02 02 00
4 SINDHUB 52/F 906676 46.4 47.4 06 48 47 03 02 00
AI
5 NOOTAN 6/F 906739 27.4 67.9 4.7 70 27 02 01 00
23. NAME A/S MRD
NO. CELL COUNTER(%) PEREPHERAL SMEAR (%)
LYMP GRA MID P L E M B
H
6 RAVI 7/M 908240 44.7 48.7 6.6 49 47 05 01 00
7 DEVASHIS 5/M 908241 50.0 42.4 7.6 47 50 02 01 00
8 SHALU 27/F 908285 31.6 63.4 5.0 65 32 02 01 00
9 MOTIRAN 72/M 908277 27.7 66.9 5.4 66 30 03 01 00
M
10 SUNITA 20/F 908266 31.0 61.5 7.5 65 32 02 01 00
24. RESULT
- As per the above observation we can see
that there isn’t much of a difference
between cell counter reading and the
reading by peripheral smear.
- Hence after the comparative study
between the two we can conclude that
cell counter reading are equally efficient
and automat zed reading scan be
preferred over the manual to save time.
25. DISCUSSION
Causes of alteration in DLC count-
1) Errors in calculation
2) Increased erythropoietin in a blood sample.
3) Improper admixture of anticoagulant in a blood sample.
4) If blood sample is clotted.
5) If sample collected in a plain blub.
6) Overstaining.
7) Suboptimal staining.
As per the causes mentioned above before going to
analysis or observe for differential leukocyte count one
must nn of all those things to avoid errors in differential
leukocyte count.
Accordingly some of the precautions to be taken by
examining the peripheral smear or analyzed by
automated cell counter