Introduction:
Proposed by Meuwissen et al. (2001)
GS is a specialized form of MAS, in which information from genotype data on marker alleles covering the entire genome forms the basis of selection.
The effects associated with all the marker loci, irrespective of whether the effects are significant or not, covering the entire genome are estimated.
The marker effect estimates are used to calculate the genomic estimated breeding values (GEBVs) of different individuals/lines, which form the basis of selection.
Why to go for genomic selection:
Marker-assisted selection (MAS) is well-suited for handling oligogenes and quantitative trait loci (QTLs) with large effects but not for minor QTLs.
MARS attempts to take into account small effect QTLs by combining trait phenotype data with marker genotype data into a combined selection index.
Based on markers showing significant association with the trait(s) and for this reason has been criticized as inefficient
The genomic selection (GS) scheme was to rectify the deficiency of MAS and MARS schemes. The GS scheme utilizes information from genome-wide marker data whether or not their associations with the concerned trait(s) are significant.
GEBV: GenomicEstimated Breeding Values-
The sum total of effects associated with all the marker alleles present in the individual and included in the GS model applied to the population under selection
Calculated on a single individual basis
Gene-assisted genomic selection:
A GS model that uses information about prior known QTLs, the targeted QTLs were accumulated in much higher frequencies than when the standard ridge regression was used
The sum total of effects associated with all the marker alleles present in the individual and included in the GS model applied to the population under selection
Calculated on a single individual basis
Population used:
Training population: used for training of the GS model and for obtaining estimates of the marker-associated effects needed for estimation of GEBVs of individuals/lines in the breeding population.
Breeding population: the population subjected to GS for achieving the desired improvement and isolation of superior lines for use as new varieties/parents of new improved hybrids.
Training population-
large enough: must be representative of the breeding population: max. trait variance with marker : by cluster analysis
should have either equal or comparable LD, LD decay rates with breeding populations
Updated by including individuals/lines from the breeding population
Training more than one generation
Low colinearity between markers is needed since high colinearity tends to reduce prediction accuracy of certain GS models. (colinearity disturbed by recombination)
Breeding strategies for nutritional quality in major cereal cropsHeresh Puren
The presentation describes about the nutritional deficiency symptoms, deficiency status at both national and global scenario which signifies the need for breeding strategies for nutritional improvement as well as the various strategies for improvement of nutritional quality in major cereal crops.
Introduction:
Proposed by Meuwissen et al. (2001)
GS is a specialized form of MAS, in which information from genotype data on marker alleles covering the entire genome forms the basis of selection.
The effects associated with all the marker loci, irrespective of whether the effects are significant or not, covering the entire genome are estimated.
The marker effect estimates are used to calculate the genomic estimated breeding values (GEBVs) of different individuals/lines, which form the basis of selection.
Why to go for genomic selection:
Marker-assisted selection (MAS) is well-suited for handling oligogenes and quantitative trait loci (QTLs) with large effects but not for minor QTLs.
MARS attempts to take into account small effect QTLs by combining trait phenotype data with marker genotype data into a combined selection index.
Based on markers showing significant association with the trait(s) and for this reason has been criticized as inefficient
The genomic selection (GS) scheme was to rectify the deficiency of MAS and MARS schemes. The GS scheme utilizes information from genome-wide marker data whether or not their associations with the concerned trait(s) are significant.
GEBV: GenomicEstimated Breeding Values-
The sum total of effects associated with all the marker alleles present in the individual and included in the GS model applied to the population under selection
Calculated on a single individual basis
Gene-assisted genomic selection:
A GS model that uses information about prior known QTLs, the targeted QTLs were accumulated in much higher frequencies than when the standard ridge regression was used
The sum total of effects associated with all the marker alleles present in the individual and included in the GS model applied to the population under selection
Calculated on a single individual basis
Population used:
Training population: used for training of the GS model and for obtaining estimates of the marker-associated effects needed for estimation of GEBVs of individuals/lines in the breeding population.
Breeding population: the population subjected to GS for achieving the desired improvement and isolation of superior lines for use as new varieties/parents of new improved hybrids.
Training population-
large enough: must be representative of the breeding population: max. trait variance with marker : by cluster analysis
should have either equal or comparable LD, LD decay rates with breeding populations
Updated by including individuals/lines from the breeding population
Training more than one generation
Low colinearity between markers is needed since high colinearity tends to reduce prediction accuracy of certain GS models. (colinearity disturbed by recombination)
Breeding strategies for nutritional quality in major cereal cropsHeresh Puren
The presentation describes about the nutritional deficiency symptoms, deficiency status at both national and global scenario which signifies the need for breeding strategies for nutritional improvement as well as the various strategies for improvement of nutritional quality in major cereal crops.
Power Point is deals with the different aspects of Quantitative genetics in plant breeding it converse Basic Principles of Biometrical Genetics, estimation of Variability, Correlation, Principal Component Analysis, Path analysis, Different Matting design and Stability so on
Rice (Oryza sativa L. 2n = 2x = 24) is a staple food for over half of the world's populationproviding 43% of calorie. Rice yield has experienced many fold jumps since the 1950s. This happened primarily as the result of genetic improvement and increasing harvest index by reducing plant height using the semi-dwarf genes and utilization of heterosis by producing hybrids. Heterosis is the improved or increased function of any biological quality in a hybrid offspring. An offspring exhibits heterosis if its traits are enhanced as a result of mixing the genetic contributions of its parents. Genetic basis of heterosis included overdominance, dominance, and additive effects.
Heterotic group “is a group of related or unrelated genotypes from the same or different populations, which display similar combining ability and heterotic response when crossed with genotypes from other genetically distinct germplasm groups.”
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
I would like to share this presentation file.
Some basics information regarding to molecular plant breeding, hope this help the beginner who start working in this field.
Thanks for many original source of information (mainly from slideshare.net, IRRI, CIMMYT and any paper received from professor and some over the internet)
Genetic Engineering of Male Sterility for Hybrid Seed Production # Methods of Hybrid Seed Production - Hybridization techniques # Examples of Male Sterile Hybrid Seed
Quality protein maize biofortification for nutritional securitynirupma_2008
Maize is a versatile crop, used as human food, livestock feed and raw material in industries. Being robust and extremely adaptable in various agro-climatic conditions, it is a favourite crop of farmers throughout the world. For majority of the population, especially rural poor maize constitutes the main bulk of the daily diet. But, the concern lies in the insufficient protein quality and quantity in maize grain leading to malnutrition. Its nutritional value is limited by the low levels of essential amino acids, particularly lysine and tryptophan. In maize endosperm, zein constitutes 50 to 70% of storage protein which is abundant in glutamine, leucine and proline but devoid of the essential amino acids viz., lysine and tryptophan (Prasanna 2001 ; Gibbon and Larkins, 2005; Wu et al., 2010). The discovery of a natural mutation called opaque2 (o2) in 1960’s, caused reduction of zein and increase in non-zein proteins in maize grain doubling the level of lysine (Mertz et al., 1964; Krivanek et al., 2007; Wu et al.,2010). However, the o2 mutation had negative pleiotropic effects that resulted in soft, chalky and dull endosperm, (Babu et al., 2005) leading to decrease in grain den¬sity, increase in susceptibility to attacks by pests and diseases and decrease in productivity. These defects were ameoliarated by the efforts of plant breeders by selecting o2 lines with hard, translucent (vitreous) kernels that retained high lysine content. These modified opaque lines had loci called “modifiers” and such genotypes were called “Quality Protein Maize” (--1,--3,--6, Ortega and Bates, 1983; Villegas et al., 1992; Toro, 2001).
Power Point is deals with the different aspects of Quantitative genetics in plant breeding it converse Basic Principles of Biometrical Genetics, estimation of Variability, Correlation, Principal Component Analysis, Path analysis, Different Matting design and Stability so on
Rice (Oryza sativa L. 2n = 2x = 24) is a staple food for over half of the world's populationproviding 43% of calorie. Rice yield has experienced many fold jumps since the 1950s. This happened primarily as the result of genetic improvement and increasing harvest index by reducing plant height using the semi-dwarf genes and utilization of heterosis by producing hybrids. Heterosis is the improved or increased function of any biological quality in a hybrid offspring. An offspring exhibits heterosis if its traits are enhanced as a result of mixing the genetic contributions of its parents. Genetic basis of heterosis included overdominance, dominance, and additive effects.
Heterotic group “is a group of related or unrelated genotypes from the same or different populations, which display similar combining ability and heterotic response when crossed with genotypes from other genetically distinct germplasm groups.”
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
I would like to share this presentation file.
Some basics information regarding to molecular plant breeding, hope this help the beginner who start working in this field.
Thanks for many original source of information (mainly from slideshare.net, IRRI, CIMMYT and any paper received from professor and some over the internet)
Genetic Engineering of Male Sterility for Hybrid Seed Production # Methods of Hybrid Seed Production - Hybridization techniques # Examples of Male Sterile Hybrid Seed
Quality protein maize biofortification for nutritional securitynirupma_2008
Maize is a versatile crop, used as human food, livestock feed and raw material in industries. Being robust and extremely adaptable in various agro-climatic conditions, it is a favourite crop of farmers throughout the world. For majority of the population, especially rural poor maize constitutes the main bulk of the daily diet. But, the concern lies in the insufficient protein quality and quantity in maize grain leading to malnutrition. Its nutritional value is limited by the low levels of essential amino acids, particularly lysine and tryptophan. In maize endosperm, zein constitutes 50 to 70% of storage protein which is abundant in glutamine, leucine and proline but devoid of the essential amino acids viz., lysine and tryptophan (Prasanna 2001 ; Gibbon and Larkins, 2005; Wu et al., 2010). The discovery of a natural mutation called opaque2 (o2) in 1960’s, caused reduction of zein and increase in non-zein proteins in maize grain doubling the level of lysine (Mertz et al., 1964; Krivanek et al., 2007; Wu et al.,2010). However, the o2 mutation had negative pleiotropic effects that resulted in soft, chalky and dull endosperm, (Babu et al., 2005) leading to decrease in grain den¬sity, increase in susceptibility to attacks by pests and diseases and decrease in productivity. These defects were ameoliarated by the efforts of plant breeders by selecting o2 lines with hard, translucent (vitreous) kernels that retained high lysine content. These modified opaque lines had loci called “modifiers” and such genotypes were called “Quality Protein Maize” (--1,--3,--6, Ortega and Bates, 1983; Villegas et al., 1992; Toro, 2001).
It is an poster prepared in Indian national language "Hindi". It describes the methodology of pure seed production by farmers himself/herself. May be useful for Indian farmers, students, scientists for providing basic idea to another.
Arvind ltd agri business brochure organic farmingArvind Limited
Arvind Agribusiness was established in 2007 with the aim of creating a more balanced and healthy ecosystem. Being a leading textile manufacturer and a major consumer of cotton, we focus to make our key resources more eco-friendly and sustainable.
Quality protein maize (QPM) combining the enhanced levels of
lysine and tryptophan with high grain yield and desirable agronomic traits could
reduce food insecurity and malnutrition in West and Central Africa. Twenty-two
varieties of QPM and two local checks were evaluated for agronomic
characteristics and nutritional qualities in the southern Guinea savanna of Nigeria
for two years (2009 and 2010). Though crude protein levels are good indicators of
quality, amino acid composition analyses, especially lysine and tryptophan that
would provide a final proof are in progress. The genotypes comprised five open
pollinated varieties (OPVs), nine inbred lines, eight hybrids and two local varieties.
Differences among the varieties were significant (P<0.01)><0.05) for days to tasselling. Most of the QPM inbred lines (CML
437, CML 490 CML 178) and hybrids (Dada-ba, ART98-SW5-OB, ART98-SW4-
OB and TZPB-OB) have superior performance for grain yield, other yield
attributes and nutritional qualities compared with the OPVs and local checks.
These inbreds could be potential sources of favorable alleles useful for future
maize breeding, while the hybrids could be evaluated in different environments of
Nigeria for comparative advantages in different environments and quality of the
grains to be released to farmers.
TEST CROSS PERFORMANCE AND COMBINING ABILITY OF QUALITY PROTEIN MAIZE (ZEA MA...Premier Publishers
The present study was conducted to assess the performance of test cross hybrids and estimate the combining ability of highland maize inbred lines for grain yield and yield-related traits. 40 crosses generated by crossing twenty lines with two testers and two genetic checks were evaluated using alpha lattice design with two replications at Ambo and Kulumsa agricultural research centers in 2019 main cropping season. Analyses of variances showed significant mean squares due to crosses for all studied traits that indicated genetic variation among the materials. GCA mean square due to lines showed significant differences for all traits. Similarly, GCA mean square due to testers was significant for all traits except anthesis-silking interval, while SCA mean squares were significant only for grain yield and number of ears per plant. Inbred lines viz., L7, L13, L5, L2 and L18 were good general combiners for yield and yield attributing characters. Tester CML159 was high combiner for grain yield than CML144. Among the hybrids, L5 x T1, L18 x T1 and L12 x T1 exhibited high mean values over checks and highest SCA effects for yield and yield attributing traits, thus could be used for further use in the breeding and cultivar development process.
Study of Intake, Growth and Nutrient Utilization of Growing Bulls Fed Forages...IJEAB
The study was conducted to rank Napier, jumbo, maize and rice straw on the basis of their yield, production cost, nutritional value and productivity of native growing bulls. Thirty native bulls (Bos indicus) of 135 (+28/-28) kg live weight (LW) were randomly allocated to five treatments in a completely randomized design and fed silage of maize (Zea mays; Hybrid, PG-1000), jumbo (Sorghum bicolor; Hybrid Sugar graze), Napier (Pennisetum perpureum; hybrid) and urea molasses straw of whole straw (UMS-WS) and UMS of stover (UMS-S) for a period of 90 days. The dry matter (DM) intake of Napier, jumbo, maize, UMS-WS and UMS-S was 2.08, 1.79, 2.01, 1.92 and 2.08 % LW, respectively which differed significantly (P<0.01).><0.01)><0.01) in bulls fed maize silage (273.3 g/d) followed by Napier silage (81.4 g/d), UMS-S (75.3 g/d), jumbo silage (39.9 g/d) and UMS-WS (39.6 g/d). Considering the cost of beef production, maize may be ranked on the top followed by Napier, jumbo, UMS-S and UMS-WS, respectively which may be taken in profitable beef production system.
Bio fortification through Genetic EngineeringBalaji Rathod
Crop Bio-fortification is the idea of breeding crops to increase their nutritional value.
Bio-fortification differs from ordinary fortification because it focuses on making plant foods more nutritious as the plants are growing, rather than having nutrients added to the foods when they are being processed.
This is an improvement on ordinary fortification when it comes to providing nutrients for the rural poor, who rarely have access to commercially fortified foods.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
1. Rapid Improvement
of
Quality Protein
Maize
Vinod Kumar Goyal & Lokesh Gour
DEPARTMENT OF PLANT BREEDING & GENETICSDEPARTMENT OF PLANT BREEDING & GENETICS
JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYAJAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
JABALPUR , MADHYA PRADESH (INDIA)JABALPUR , MADHYA PRADESH (INDIA)
2. • Name of Crop: Maize
• Botanical Name: Zea mays L.
• Family: Poaceae
• Chromosome No.: 2n = 20
• Center of Origin: Central America (Mexico)
• Mode of pollination : Cross pollination
• Out crossing percentage: 95%
•India is the sixth largest producer of maize in the world contributing 2%
of the global production.
•In India maize is the third most important crop after rice and wheat
and accounts for 9% of the total food grain production in country.
•The important maize growing states are Karnataka, Andhra Pradesh,
Bihar, Punjab and Himachal Pradesh
Maize ?
3. • Primarily animal feed in
– East and Southeast Asia
• Primarily human food in
– Africa
– Central America
– South Asia
• 15-56% of total daily calories
– For several hundred million
people
– Including weaning children
– in 25 countries
Maize in developing countries
4. Nutritional
Limitations of Maize
Low availability of
niacin
Low lysine and
tryptophan
Need to improve
nutrition
– Essential amino acids
– Major seed protein (60%)
– Lysine level about 2%
• 4% recommended by
FAO
– For humans and animals
– Quality Protein Maize:
QPM
5. Quality Protein Maize
It is an improved variety of
maize which contains higher
amount of lysine and
tryptophan with lower amount
of leucine and isoleucine in
the endosperm than those
contained in normal maize.
6. Need Of Quality Protein Maize
However, malnutrition still remains a widespread problem, and is
particularly severe in developing countries with low per capita income.
Animal protein, of course being of higher quality, is scarce and expensive,
thereby unavailable to a vast sector of the population.
Maize is a major cereal crop and plays very important role in human and
animal nutrition in a number of developed and developing countries
worldwide, derive their protein and calorie requirements from maize.
With its high content of carbohydrates, fats, proteins, some of the important
vitamins and minerals, maize acquired a well-deserved reputation as a “poor
man’s nutria-cereal”.
Normal maize varieties are deficient to two essential amino acids, lysine and
tryptophan (Azevedo and Arruda, 2010; Mbuya et al., 2011).
11. Nutritional superiority of QPM: A
compression
Lysine content
QPM protein
contains
Biological
Value
Normal -1.3 g per 100 g endosperm protein.
o2-3.3 to 4.0 g per 100 g of endosperm protein.
55% more tryptophan.
30% more lysine.
38% less leucine than that of normal maize.
Normal maize protein is 45%.
o2 maize is 80%..
12. Protein intake
utilization
Maize required
for nitrogen
equilibrium/ kg
of body wt.
Other
nutritional
benefits
Common maize - 37%
o2 maize protein -74%
Normal maize -24 g
QPM-8 g.
Higher tryptophan and lower leucine content,
Higher calcium and carbohydrate and carotene
Higher niacin
Cont.
14. Opaque2 – a gene for improving quality of protein
in maize
A natural spontaneous maize mutant with soft and opaque grain was
found in a maize fields in USA during the 1920s which was later
named as opaque2 (o2) maize by Singleton.
The mutant was passed onto Mertz at Purdue University, USA, who,
in turn, reported that the o2 homozygous maize contained
substantially higher lysine (+69%) in the grain endosperm compared
to normal maize.
The increase in lysine content doubled the biological value of the o2
maize protein and this increase in protein quality is due to increase in
the ratio of non-zein to zein proteins.
15. Early efforts and experiences in using o2 cultivars
Initiation of Breeding programmes to develop inbred lines by using
Populations with Endosperm quality mutants, mainly o2
16. o2 utilization in breeding programmes
Resulted
Soft endosperm
Damaged kernels
Susceptibility to
pests and fungal
diseases
Inferior food processing
Reduced yields
Early efforts and experiences in using o2 cultivars
pleiotropic effects of this gene
17. Development of QPM involves three genetic systems, viz.
I. Recessive homozygous allele of the o2 gene
II. Modifiers for kernel hardness
III. Amino acid modifiers
Genetic systems and their role in enhancing the level of
limiting amino acids in QPM
18. • The o2 allele in homozygous condition reduces production of alpha-
zeins and triggers increase in the level of lysine and tryptophan.
• Involved in the synthesis of the
enzyme that is associated with
free lysine degradation.
Reduction in this enzyme leads
to a corresponding increase in
free lysine in the endosperm.
(I ) Recessive homozygous allele of the o2 gene
Reduced protein body size in o2
WT
o2
19. ) Modifiers for kernel hardness
• Increased level of the gamma-zein is likely to contribute to the
recovery of hard endosperm.
• o2 modified (QPM) grains have double the amount of gamma-zeins
in the endosperm compared to the o2 mutants
• Two genes responsible for the grain hardness, mapped to the long
arm of chromosome 7 and one of them is located near the gamma-
zein gene ‘gzr 1’.
20. • Increased amount of granule-bound starch synthase I reflected in the
form of a change in starch structure, which was manifested as shorter
amylopectin branches and increased swelling of starch granules.
CM105 wild-type CM105 opaque 2
Starch
Starch
PB
21. ) Amino acid modifiers
• It also affect the relative level of lysine and tryptophan content in
grain endosperm.
• Three genes associated with lysine level have been mapped to
locations on chromosome 2, 4 and 7.
• Apart from this, several major o2 modifier-QTLs located on
chromosomes 1, 7 and 9, have been recently mapped
22. A.Gradual increase in proportion of
soft to hard endosperms
Tucson (USA) Lopes and Larkin (1991)
B. Spatial expression of opaque-2
under different genetic backgrounds
24. Rapid Improvement of QPM genotypes through
conventional breeding
Development of Acceptable QPM lines by combining the nutritional
advantages of o2 mutation with the o2 modifiers.
During the 1980s, CIMMYT took initiatives to convert a number of
non-QPM genotypes to QPM genotypes, they followed a ‘modified
backcrossing-cum-recurrent selection’.
During the conversion process, they also emphasized grain yield,
kernel modification, reduced ear rot incidence and other agronomic
traits.
In a short span of 5–6 years, CIMMYT could convert many normal
germplasm into QPM, which were as good as their non-QPM
counterparts for grain yield and other agronomic traits.
25. Two scientists of CIMMYT, Mexico, Dr. S. K. Vasal and Dr.
Evangelina Villegas, for a period of three decades led to development
of Quality Protein Maize (QPM) with hard kernel, good taste and other
consumer favouring characteristics.
QPM research and development spread from Mexico to Central and
South America, Africa, Europe and Asia.
Awarded 2000 “World food prize” for path breaking research.
Cont.
26. 1970 -India is one of the first few countries to focus on o2 maize and
released three o2 composites, namely Shakti, Rattan and Protina .
1997- modified superior o2 composite ‘Shakti 1’.
Later, India released eight QPM hybrids, seven of which were
developed from the QPM inbreds of CIMMYT as parental lines and
are of full season maturity.(HQPM1, HQPM5 ,HQPM 7 Vivek QPM
9)
Breakthrough achievement in development of “Miracle Maize”
Cont.
27. Development of QPM hybrid through marker assisted
selection
In order to shorten the period required for development of QPM
hybrids through the conventional method of backcrossing, marker-
assisted selection (MAS) can be used.
Few molecular markers were already known within the o2 gene and
these makers were capable of detecting the o2 gene even in hetero-
zygous state.
To convert normal maize hybrid into QPM hybrid, a promising hybrid,
viz. Vivek Maize Hybrid 9 (developed by Vivekananda Parvatiya Krishi
Anusandhan Sansthan, Almora) was selected for converting into QPM.
28. CML 180
VQL 1
CML 170CM 212
VQL 2
CM 145
Recipient
o2gene
Recipients
o2gene
Donor
inbred line
Donor
inbred line
Vivek QPM 9
(92.0%) (94.44%)
o2o2 quantitative
determination
Using SSR markers
phi57, phi112,
umc 1066
Determining lysine, tryptophan , protein
Biochemical analysis
Conversion of normal maize inbreds into QPM
Gupta et al. 2009.
29. The hybrid was released for commercial cultivation in Himalayan
states, Andhra Pradesh, Tamil Nadu, Karnataka and Maharashtra by
the Central Seed Sub-committee on Crop Standard and Notification
in the year 2000.
Cont….
30. Name Pedigree Year of
Release
Maturity
group
Centre’s
name
Shakti Composite 1970 Full season AICRP
Rattan Composite 1970 Full season AICRP
Protina Composite 1970 Full season AICRP
Shakti 1 Composite 1997 Full season DMR
Shaktiman 1 (CML 142 x CML 150) xCML 186 2001 Full season Dholi
Shaktiman 2 CML 176 x CML 186 2004 Full season Dholi
HQPM 1 HKI 193-1 x HKI 163 2005 Full season Uchani
Shaktiman 3 CML 161 x CML 163 2006 Full season Dholi
Shaktiman 4 CML 161 x CML 169 2006 Full season Dholi
HQPM 5 HKI 163 x HKI 161 2007 Full season Uchani
HQPM 7 HKI 193-1 x HKI 161 2008 Full season Uchani
Vivek QPM 9 VQL 1 x VQL 2 2008 Extra early Almora
QPM cultivars released for commercial cultivation
in India
(Dhillon and Prasanna, 2001 and Gupta et al. 2009
31. Name Protein content in
grain (%)
Tryptophan content in
protein (%)
Shaktiman1 9.6 1.01
Shaktiman 2 9.3 1.04
HQPM 1 9.36 0.94
Shaktiman 3 9.63 0.73
Shaktiman 4 9.98 0.93
HQPM 5 9.80 0.76
HQPM 7 9.4 0.72
Vivek QPM 9 8.46 0.83
Protein content in grain (%) and Tryptophan content
in protein (%)
32. Recently developed QPM varieties
Following this approach, we have now developed another elite QPM
hybrid-FQH 38
The QPM version of Vivek Maize Hybrid 21.
The new QPM hybrid contains more than 72% increase in tryptophan
over Vivek Maize Hybrid 21
Anand QPM 1- developed from Anand agriculture university.
33. For a country like India, with diverse agroclimatic and soil situations,
need to develop a number of QPM hybrids of different maturity
groups, viz. early, medium and late (full season).
However, the major constraints in adoption of the QPM hybrids in
these areas are the non-availability of hybrid seeds and lack of
incentives like premium price for the QPM over normal maize grains.
There is also a need to create awareness among the consumers and
industry for its use in food and feed.
We are developing a linkage between the seed producers, farmers and
the industry to bring about the much needed synergy in development
and utilization of QPM that will help in reducing protein
Conclusion
34. Anonymous 2008. The Gazette of India, Extraordinary. Part II. Section
3, subsection (ii), No. 1448, Serial No. 28, Ministry of Agriculture,
Department of Agriculture and Cooperation, New Delhi, 16
October.
Babu R. 2005. Two generation marker-aided backcrossing for rapid
conversion of normal maize lines to quality protein maize (QPM).
Theor. Appl. Genet. (111): 888–897.
Sharma R, Maloo S R and Joshi A. 2014. Genetic variability analysis in
diverse maize genotypes. Journal of plant breeding 5(3):545-551.
Some of Referances
35. Vasal SK, Villegas E, Bjarnason M, Gelaw B, and Goertz P. 1980.
Genetic Modifiers and Breeding Strategies in Developing Hard
Endosperm opaque-2 Materials, pp. 37–73 in Improvement of
Quality Traits of Maize for Grain and Silage Use, edited by
Pollmer WG and Phipps RH. Nijhoff M, London.
Yadav VK. 2001. Quality protein maize. In Production Technology and
Value Addition, Directorate of Maize Research, New Delhi, p. 4.
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