Post translation modifications(molecular biology)IndrajaDoradla
description of post translation modifications which include folding,proteolytic clevage and chemical modification and protein splicing and protein degradation
Post translation modifications(molecular biology)IndrajaDoradla
description of post translation modifications which include folding,proteolytic clevage and chemical modification and protein splicing and protein degradation
An Overview...
Definition of Translation.
Def. of Eukaryotes.
Translation: An Overview.
Components of Translation.
Some Enzymes .
Ribosome Role.
Mechanism of Translation.
Initiation.
Scanning Model of Initiation.
Initiation Factors.
Animation.
Elongation.
Chain Elongation: Translocation.
Animation.
Termination.
Animation....
It's not perfect still... what are your views friends?
description of translation in both prokaryotes and eukaryotes and the components required for translation and also co translation tranlocation,post translation translocation and also inhibitors of translation in both prokaryotes and eukaryotes
Structure and function of Messenger RNA (mRNA )ICHHA PURAK
This presentation of 42 slides delivers information about structure,function synthesis , life span of both prokaryotic and eukaryotic messenger RNA also about role in protein sorting and targetting
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
Protein synthesis and processing: Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, Post Translational modification of proteins. Protein targeting.
Prokaryotic translation machinery by kk KAUSHAL SAHU
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
An Overview...
Definition of Translation.
Def. of Eukaryotes.
Translation: An Overview.
Components of Translation.
Some Enzymes .
Ribosome Role.
Mechanism of Translation.
Initiation.
Scanning Model of Initiation.
Initiation Factors.
Animation.
Elongation.
Chain Elongation: Translocation.
Animation.
Termination.
Animation....
It's not perfect still... what are your views friends?
description of translation in both prokaryotes and eukaryotes and the components required for translation and also co translation tranlocation,post translation translocation and also inhibitors of translation in both prokaryotes and eukaryotes
Structure and function of Messenger RNA (mRNA )ICHHA PURAK
This presentation of 42 slides delivers information about structure,function synthesis , life span of both prokaryotic and eukaryotic messenger RNA also about role in protein sorting and targetting
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
Protein synthesis and processing: Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, Post Translational modification of proteins. Protein targeting.
Prokaryotic translation machinery by kk KAUSHAL SAHU
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
CBCS 4TH SEM ,
CHARGING, STRUCTURE AND FUNCTION OF tRNA,
AMINOACYL RNA SYNTHETASE(ASR) PROOFREADING AND EDITING
https://www.youtube.com/watch?v=YzOVMWYLiCE
This presentation includes process of protein synthesis for B.Sc. level. Presentation shows stepwise process for easy understanding.Many steps includes very simple non-grahical figures scanned from the book.
It is the process of synthesis of protein by encoding information on mRNA.
Protein synthesis requires mRNA, tRNA, aminoacids, ribosome and enzyme aminoacyl tRNA synthase
INTRODUCTION
HISTORY
MECHANISM OF PROTEIN SYNTHESIS
TRANSCRIPTION
TRANSLATION
TRANSCRIPTION
INITIATION
ELONGATION
TERMINATION
TRANSLATION
AMINOACYLATION OF tRNA
INITIATION OF POLYPEPTIDE CHAIN
ELONGATION
TERMINATION
CONCLUSION
REFERENCES
Topics covered are:
1. History and Characteristics of Genetic codes
2. Wobble hypothesis
3. Stages (Initiation, Elongation and Termination) of translation in Prokaryotes and Eukaryotes with enzymes and their functions
4. Post-translation modification such as Glycosylation, Lipidation, Phosphorylation, Acetylation, Methylation (lysine and arginine methylation) and Ubiquitination
This is a process by which the genetic code contained within a messenger RNA (mRNA) molecule is decoded to produce a specific sequence of amino acids in a polypeptide chain.
Translation is the process of translating the sequence of a messenger RNA (mRNA) molecule to a sequence of amino acids during protein synthesis. The genetic code describes the relationship between the sequence of base pairs in a gene and the corresponding amino acid sequence that it encodes.
Multiple Choice Questions with Explanatory Answers on Chemistry of Carbohydrates for Medical, Biochemistry and Biology students - Chapter 1 of Multiple Choice Questions in Biochemistry by RC Gupta
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
2. An overview of protein synthesis
A gene consists of a specific sequence
of nucleotides
Information about its amino acid
sequence is present in a gene
Every protein has got a unique amino
acid sequence
3. The base sequence of the gene encodes
a specific sequence of amino acids
When a protein is to be synthesized, the
corresponding gene is transcribed
An mRNA molecule is formed
4. The base sequence of mRNA is comple-
mentary to the sense strand of the gene
The code words on mRNA are known as
codons
The mRNA goes to cytosol and binds to
a ribosome
5. Amino acids are present in cytosol
They bind to specific tRNA molecules
This is known as charging of tRNAs
6. Each tRNA possesses an anticodon for a
particular amino acid
The anticodon is complementary to a
codon
The charged tRNAs go to the ribosome
7. Charged tRNAs find the complementary
codons on mRNA
Amino acids are joined in a sequence
directed by the codons on mRNA
This process is called translation
8. Structural gene
Transcription
3´ hnRNA
3´ mRNA
40S Subunit
of ribosome
60S Subunit
of ribosome
5´
tRNAs
DNA
Addition of cap and tail, and splicing
5´
5´
3´
a1
a2
an
+ + +
Amino acids Amino acyl tRNAs
+
a1 a2
a3
an an
a2a1
a3
9. Gene expression means using the
information present in the gene to
synthesize a protein
Gene expression
A gene is expressed when the protein
encoded by it is required
10. Gene expression
comprises four steps:
Transcription of gene
Charging of tRNAs
Translation
Post-translational modifications
11. For expression of a gene, the coded infor-
mation present in it has to be transcribed
Transcription of gene
In eukaryotes, primary transcript is
hnRNA, which is processed to form mRNA
In prokaryotes, the primary transcript is
mRNA
12. The mRNA comes out of the nucleus and
attaches to a ribosome
The amino acids are incapable of
recognizing the codons on the mRNA
An adaptor molecule is required to match
the amino acid and the codon
13.
14. The adaptor molecule is tRNA
Amino acid
binding site
Amino acid
binding site
Acceptor
arm
Acceptor
arm
Pseudouridine
loop
Dihydrouracil
loop
Anticodon
loop
Anticodon
loop
5’
15. For protein synthesis, the amino acids have
to be carried from cytosol to ribosomes
The adaptor molecule (tRNA) is the carrier
of amino acids
First, the amino acids have to be attached
to tRNAs
Charging of tRNAs
16. Each tRNA has an anticodon loop
The anticodon loop contains an anti-
codon
The anticodon is complementary to a
particular codon
17. A given tRNA can combine with only one
amino acid
The amino is selected according to the
anticodon present on the tRNA
18. Binding of amino acid to tRNA is known
as charging of tRNA
The binding is catalysed by amino acyl
tRNA synthetase
The amino acid, ATP and the enzyme
react to form amino acyl-AMP-enzyme
complex
19. Amino acyl-AMP-enzyme complex reacts
with tRNA to form amino acyl tRNA
Enzyme, AMP and PPi are released; PPi
is hydrolysed
Amino acyl tRNA is known as charged
tRNA
20. II
Amino acyl tRNA
R−CH−C−tRNA
NH2 O
R−CH−COOH + ATP + Enzyme
NH2
Amino acid
II
Amino acyl-AMP-Enzyme complex
R−CH−C−AMP−Enzyme
ONH2
PPi
H2O
2 Pi
tRNA
AMP + Enzyme
21. The tRNA carries the amino acid to
ribosome
The anticodon on tRNA finds the
complementary codon on mRNA
Amino acids are added in a sequence
directed by the codons on mRNA
22. Twenty amino acids are required for
protein synthesis
Therefore, there must be at least 20
species of tRNA
23. Amino acid is bound to 3’-end of tRNA;
the anticodon is present far away
Therefore, the anticodon does not play
any role in recognition of amino acid
This function is performed by the enzyme,
amino acyl tRNA synthetase
24. There are at least 20 different species of
amino acyl tRNA synthetase
Each amino acyl tRNA synthetase charges
one tRNA with a specific amino acid
Once a tRNA is charged, its anticodon will
find the complementary codon on mRNA
25. One experiment showed that amino acid
has no role in recognition of codon
In this experiment, the tRNA for cysteine
(tRNAcys) was charged with cysteine
The cysteine residue was then chemically
converted into an alanine residue
26. Anticodon of this tRNA recognized the
codon for cysteine
Raney
nickel
[S]
CH2 –CH–C–tRNA
O
||
|
NH2
|
SH
Cysteine
CH3–CH–C–tRNA
O
||
|
NH2
Alanine
Therefore, this tRNAcys added alanine in
place of cysteine during translation
27. When an anticodon pairs with a codon,
the first two bases of the codon are
recognized precisely
Recognition of the third base is not
precise; the third base may be mis-paired
This is known as wobble in base pairing
Wobble
29. Due to degeneracy, codons usually differ
in the third base
They will be read as code words for the
same amino acid because of wobble
Therefore, correct amino acid will be
incorporated in the protein
30. The actual process of protein synthesis is
known as translation
Translation occurs on ribosomes
It can be divided into: (i) initiation, (ii)
elongation and (iii) termination
Translation
31. Initiation is binding of mRNA and the first
amino acyl tRNA to the ribosome
Elongation is addition of subsequent
amino acids to the first one
Termination is conclusion of elongation
and release of the polypeptide
32. Initiation
Initiation of protein synthesis requires the
interaction of:
• Ribosome
• mRNA
• First amino acyl tRNA
• GTP
• ATP
• Eukaryotic initiation factors (eIFs)
33. Initiation occurs in four steps:
• Dissociation of ribosomal subunits
• Formation of 43S pre-initiation complex
• Formation of 48S initiation complex
• Formation of 80S initiation complex
34. Dissociation of ribosomal subunits
The 80S ribosome dissociates into its
40S and 60S subunits
Dissociation occurs in the presence of
eIF-1A and eIF-3
eIF-1A and eIF-3 bind to 40S subunit,
and prevent its re-association with 60S
subunit
36. Formation of 43S pre-initiation complex
In the presence of eIF-2C, eIF-2 and GTP
bind to the first amino acyl tRNA
In eukaryotes, the first amino acyl tRNA is
always methionyl tRNA
This complex binds to 40S subunit to form
the 43S pre-initiation complex
38. Formation of 48S initiation complex
eIF-4F binds to the 5’ cap of mRNA
eIF-4A and eIF-4B bind to mRNA in the
presence of ATP
IF-4A hydrolyses ATP into ADP and Pi
Using this energy, eIF-4B uncoils the
mRNA near its 5’-end
44. In the presence of eIF-5, 60S ribosomal
subunit binds to 40S subunit to form the
80S initiation complex
80S Initiation complex consists of 80S
ribosome, mRNA and methionyl tRNA
Formation of 80S initiation complex
45. After the formation of 80S initiation
complex, all the eIFs are released
The GTP attached to eIF-2 is hydro-
lysed into GDP and Pi
51. The 60S ribosomal subunit has got P
(peptidyl) site and A (amino acyl) site
After initiation, P site is occupied by
methionyl amino acyl tRNA; A site is vacant
Met
A
site
52. Amino acyl tRNA having anticodon
complementary to the second codon
comes
eEF-1A and GTP are attached to the
amino acyl tRNA
This complex binds to the ribosome; the
second amino acid is at the A site
54. After binding of the second
amino acyl tRNA to ribosome:
GTP is hydrolysed
eEF-1A:GDP complex
and Pi are released
55. This activity is present in the 28S rRNA
which is a ribozyme
The 60S ribosomal subunit possesses
peptidyl transferase activity
This enzyme forms a peptide bond between
carboxyl group of first amino acid and
amino group of second amino acid
59. eEF-2 translocates the mRNA along the
ribosome by one-codon distance
The dipeptide moves to the P site, and
the A site becomes vacant
Hydrolysis of GTP into GDP and Pi
provides the energy for translocation
61. A new cycle of elongation begins
The dipeptide is converted into a tri-
peptide
This process continues until all the codons
on mRNA have been translated
62. Four ATP equivalents are spent
for forming each peptide bond:
Two for charging of tRNA
Two for each cycle of elongation
63. Termination occurs when a nonsense
codon appears on the mRNA
Nonsense codons have no comple-
mentary anticodons
Nonsense codon cannot be recognized
by any tRNA
Termination
64. When there is a nonsense codon
opposite A site, the A site cannot be
occupied by any amino acyl tRNA
Instead, this site is occupied by a
eukaryotic releasing factor (eRF) and
GTP
65. In the presence of eRF and GTP,
peptidyl transferase has a different
catalytic activity
It hydrolyses the bond between the
carboxyl group of the last amino acid
and the tRNA
66. The polypeptide and the mRNA are
released from the ribosome
GTP is hydrolysed into GDP and Pi, which
are released
The eRF and the last tRNA are released
The ribosome becomes free
67. GTP
++ GDP + Pi
tRNAPolypeptide
Ribosome
+
mRNA
43
STOP
STOP
eRF
GTP
eRF
eRF
68. As chain elongation occurs, the 5’-end of
mRNA emerges from the ribosome
A new ribosome can attach to it
Thus, several ribosomes can translate
the mRNA simultaneously
Polysome
69. Polysome
5’ 3’
A number of ribosomes attached to a
mRNA constitute a polyribosome or
polysome
70. Many newly-synthesized proteins are
functionally inactive
They require some modifications in their
structure before they become active
These modifications are known as post-
translational modifications
Post-translational modifications
72. Cleavage
The nascent protein may contain some
extra amino acids which are removed
An example is removal of connecting
peptide from proinsulin to form insulin
75. Alternative cleavage of POMC yields
eight different peptides
Another example of cleavage is pro-
opio-melano-cortin (POMC)
76. Hydroxylation
Hydroxylation of proline and lysine
residues is important in the conversion
of pro-collagen into collagen
Some amino acid residues, e.g. proline
and lysine, may be hydroxylated after
translation
77. Glutamate residues of pre-prothrombin
are carboxylated after translation
Carboxylation
Glutamate residues of several other
proteins are also carboxylated
This converts pre-prothrombin into
prothrombin
78. Serine, threonine and tyrosine residues
of some proteins can be phosphorylated
Phosphorylation
Phosphorylation of tyrosine residues is
important in intracellular signaling
Phosphorylation is a method used for
regulating the activity of some enzymes
79. Carbohydrate prosthetic groups are
added to many proteins
Glycosylation
These can form O-linked, N-linked and
GPI-linked glycoproteins
Examples are mucin, immunoglobulins,
hCG etc
80. Phosphatidyl inositol in GPI-linked
glycoproteins
Amide group of asparagine in
N-linked glycoproteins
Hydroxyl group of serine in
O-linked glycoproteins
The linkage between carbohydrate
portion and protein occurs through:
81. Addition of other prosthetic groups
Several other groups are added
to proteins after translation e.g.
Haem
Flavin nucleotides
Biotin
Retinal
Metals
82. Prokaryotic translation differs from
eukaryotic translation in:
• Primary transcript
• Number of cistrons
• Number of initiation sites
• Initiator amino acid
• Initiation factors
• Elongation factors
• Releasing factors
Prokaryotic translation
83. In prokaryotes, the primary transcript is
mRNA which is directly translated
Translation can begin even before the
transcription is complete
Ribosomes can bind to partially synthe-
sized mRNA , and protein synthesis can
begin
Primary transcript
84. A cistron is the coding unit for one
polypeptide
Some of the prokaryotic mRNAs are
polycistronic
Therefore, a number of polypeptides
can be formed from one mRNA
Number of cistrons
85. Number of initiation sites
If the mRNA is polycystronic, it will
have more than one initiation sites
Besides initiator AUG, there may be
AUG codons for internal methionine
residues
AUG is the initiator codon in pro-
karyotes also
88. Shine-Dalgarno sequence is present on
mRNA
A 3-9 base complementary sequence is
present in the prokaryotic16S rRNA
The 16S rRNA binds to the Shine-
Dalgarno sequence of mRNA
It positions the mRNA correctly on the
ribosome for initiation of translation
90. In prokaryotes as well as eukaryotes,
initiator amino acid is methionine
But in prokaryotes, initiator methionine is
formylated
It becomes formylmethionine
91. Formylation of methionine is catalysed
by formyl transferase
The formyl group is provided by N10-
formyl-tetrahydrofolate
Formylation occurs after methionine has
been bound to its tRNA
92. There are only three initiation factors in
prokaryotes
These are IF-1, IF-2 and IF-3
Initiation factors
93. There are three elongation factors in
prokaryotes
These are EF-ts, EF-tu and EF-G
EF-G is analogous to eEF-2 of eukaryotes
Elongation factors
94. There are three releasing factors in
prokaryotes
These are RF-1, RF-2 and RF-3
Either RF-1 and RF-3 or RF-2 and RF-3,
are required to terminate translation
Releasing factors
95. Protein folding
This requires extensive folding of the
polypeptide chain
The newly formed primary structure has
to acquire:
Sometimes quaternary structure
Tertiary structure
Secondary structure
96. However, spontaneous folding is a
slow process
The nascent protein will fold by itself
If a correct primary structure has been
formed:
It will attain the correct conformation
It will attain higher orders of structure
98. Enzymes involved in protein folding
Protein
disulphide
isomerase
It ensures that the disulphide
bonds are formed between
the correct cysteine residues
Peptidyl prolyl
cis-trans
isomerase
It ensures that the bonds
involving proline residues
are cis or trans as required
99. The chaperone proteins are:
Heat shock proteins 40 and 70
(HSP 40 and HSP 70) in cytosol
Heat shock proteins 10 and 60
(HSP 10 and HSP 60) in mitochondria
Calnexin and calreticulin
in endoplasmic reticulum
101. Protein targeting
The proteins synthesized on ribosomes
have different destinations such as:
• Mitochondria
• Lysosomes
• Nucleus
• Cell membrane
• Export from the cell
102. The signal that directs the protein to its
destination is inbuilt in the protein
molecule
This signal is like an address written on
the protein molecule
103. Addition of mannose-6-phosphate to a
protein directs it to lysosomes
If mannose-6-phosphate is not added,
lysosomal enzymes fail to reach the
lysosomes
This results in inclusion cell disease
104. Signal hypothesis
Gunter Blobel showed how
proteins are exported from
the cell
He proposed the signal hypothesis
which is now proven
105. Signal sequence is also known as
leader peptide or signal peptide
Presence of signal sequence directs
the protein to cell membrane or
export outside the cell
It is a sequence of 15-30 amino
acids at the N-terminus of the protein
106. Signal recognition particle (SRP) is a
complex of 7S RNA and six polypeptides
When signal sequence emerges from
the ribosome, it is recognized by signal
recognition particle
SRP binds the signal sequence
107. SRP receptor is also known as the
docking protein
SRP also binds to SRP receptor on the
endoplasmic reticulum (ER)
It is present on the external side of ER
108. Ribosome receptor is present on
the external surface of ER
The ribosome synthesizing the protein
is bound to a ribosome receptor on ER
The signal sequence is directed into
ER through translocon
Translocon is a protein-conducting
channel
109. The nascent protein is usually glycosyl-
ated and transferred to Golgi apparatus
The signal sequence is split off by
signal peptidase present in ER
From there, it is either directed to cell
membrane or is exported from the cell
110.
111. Inhibitors of translation
• Translation can be inhibited by a
number of compounds
• Inhibitors may act only on prokaryotes
or eukaryotes or on both
• Inhibitors acting only on prokaryotes
can be used as antibiotics
113. Streptomycin
It also causes misreading of codons on
mRNA
Prokaryotic translation begins with the
binding of formylmethionyl tRNA to 30S
ribosomal subunit
Streptomycin inhibits this binding and
prevents initiation
114. Some other antibiotics acting
like streptomycin are:
• Neomycin
• Kanamycin
• Gentamycin
116. Chloramphenicol is an inhibitor of peptidyl
transferase activity of 23S rRNA
Chloramphenicol
Inhibition of peptidyl transferase prevents
the formation of peptide bonds
This rRNA is present in 50S ribosomal
subunit in prokaryotes
117. Erythromycin is an inhibitor of EF-G,
the analogue of eukaryotic eEF-2
Erythromycin
This blocks elongation
Inhibition of EF-G prevents translocation
of mRNA along the ribosome