Soxhlet extraction is a continuous process of extraction with a hot organic solvent. Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent.
Soxhlet extraction is a continuous process of extraction with a hot organic solvent.
Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent.
Soxhlet extraction is a continuous process of extraction with a hot organic solvent. Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent.
Soxhlet extraction is a continuous process of extraction with a hot organic solvent.
Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent.
Estimation of reducing and non reducing sugarJasmineJuliet
Reducing sugar definition and example, non-reducing sugar definition and example, Estimation of reducing sugar by DNSA method, Estimation of total sugars by anthrone metod, Estimation of non-reducing sugar from amount of total sugars and reducing sugar, formula for estimation of non-reduci
Alkaloids are a group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms. This group also includes some related compounds with neutral and even weakly acidic properties. Some synthetic compounds of similar structure are also termed alkaloids. In addition to carbon, hydrogen and nitrogen, alkaloids may also contain oxygen, sulfur and, more rarely, other elements such as chlorine, bromine, and phosphorus.
Alkaloids are produced by a large variety of organisms including bacteria, fungi, plants, and animals. They can be purified from crude extracts of these organisms by acid-base extraction. Alkaloids have a wide range of pharmacological activities including antimalarial (e.g. quinine), antiasthma (e.g. ephedrine), anticancer (e.g. homoharringtonine),cholinomimetic (e.g. galantamine), vasodilatory (e.g. vincamine), antiarrhythmic (e.g. quinidine), analgesic (e.g. morphine),antibacterial (e.g. chelerythrine), and antihyperglycemic activities (e.g. piperine). Many have found use in traditional or modern medicine, or as starting points for drug discovery. Other alkaloids possess psychotropic (e.g. psilocin) and stimulant activities (e.g. cocaine, caffeine, nicotine, theobromine), and have been used in entheogenic rituals or as recreational drugs. Alkaloids can be toxic too (e.g. atropine, tubocurarine). Although alkaloids act on a diversity of metabolic systems in humans and other animals, they almost uniformly evoke a bitter taste
Buffers are compounds or mixtures
of compounds that by their presence
in the solution resist changes in the
pH upon the addition of small
quantities of acid or alkali.
Science initiative Tutoring Service is an Staten Island, NY-based company that offers tutoring services for a wide variety of subjects. The company is currently a sole proprietorship, however the business plans to change its organizational form to an based LLC within the next year or two.
Science initiative Tutoring Service was founded by Robert D. Craig, Ph.D while Craig was teaching
Estimation of reducing and non reducing sugarJasmineJuliet
Reducing sugar definition and example, non-reducing sugar definition and example, Estimation of reducing sugar by DNSA method, Estimation of total sugars by anthrone metod, Estimation of non-reducing sugar from amount of total sugars and reducing sugar, formula for estimation of non-reduci
Alkaloids are a group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms. This group also includes some related compounds with neutral and even weakly acidic properties. Some synthetic compounds of similar structure are also termed alkaloids. In addition to carbon, hydrogen and nitrogen, alkaloids may also contain oxygen, sulfur and, more rarely, other elements such as chlorine, bromine, and phosphorus.
Alkaloids are produced by a large variety of organisms including bacteria, fungi, plants, and animals. They can be purified from crude extracts of these organisms by acid-base extraction. Alkaloids have a wide range of pharmacological activities including antimalarial (e.g. quinine), antiasthma (e.g. ephedrine), anticancer (e.g. homoharringtonine),cholinomimetic (e.g. galantamine), vasodilatory (e.g. vincamine), antiarrhythmic (e.g. quinidine), analgesic (e.g. morphine),antibacterial (e.g. chelerythrine), and antihyperglycemic activities (e.g. piperine). Many have found use in traditional or modern medicine, or as starting points for drug discovery. Other alkaloids possess psychotropic (e.g. psilocin) and stimulant activities (e.g. cocaine, caffeine, nicotine, theobromine), and have been used in entheogenic rituals or as recreational drugs. Alkaloids can be toxic too (e.g. atropine, tubocurarine). Although alkaloids act on a diversity of metabolic systems in humans and other animals, they almost uniformly evoke a bitter taste
Buffers are compounds or mixtures
of compounds that by their presence
in the solution resist changes in the
pH upon the addition of small
quantities of acid or alkali.
Science initiative Tutoring Service is an Staten Island, NY-based company that offers tutoring services for a wide variety of subjects. The company is currently a sole proprietorship, however the business plans to change its organizational form to an based LLC within the next year or two.
Science initiative Tutoring Service was founded by Robert D. Craig, Ph.D while Craig was teaching
Buffer solutions from LobaChemie are ready-to-use solutions manufactured in large lots that will save you the time and expense of preparation and standardization.this solutions are used in chemical analysis so visit https://goo.gl/OK0z65 TO know more.
preparation of buffers, buffers and isotonic systems. Methods for adjustment of tonicity of solutions. Buffers in pharmaceutical and biological systems.
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Properties of buffers
1. Properties of Buffers
Introduction
Buffers resist changes in pH when acids or bases are added to them. An effective buffer
system contains significant quantities of a specific weak acid and its conjugate base. There
are two common methods used to prepared a buffer. One method is to combine
approximately equal quantities of an acid and its conjugate base. The other method is to
convert about half of the weak acid in a solution or half of the weak base in a solution to its
conjugate form by adding a strong base or strong acid respectively. This process is called
partial neutralization. The [H+] is calculated from equation (1) where HA is a weak acid and
A- is the conjugate base.
[H+] = Ka [HA]/[A–] (1)
pH may be calculated from the Henderson–Hasselbalch equation. This is the logarithmic
form of equation (1). Two equivalent forms of the equation are:
(2)
In this experiment you will prepare a buffer and measure its pH with a pH-meter. The weak
acid of the buffer will be acetic acid (HC2H3O2) and its conjugate base, the acetate
ion(C2H3O2
–). The buffer solution will be prepared using both methods described above.
You will measure the pH as a strong acid and base are added to the buffer solution, and
compare the results with measurements made when a strong acid and base are added to
plain water and to dilute buffer solutions.
Note: The symbol HOAc is often used to represent acetic acid and OAc– to represent the
acetate ion.
Procedure:
Special equipment:
pH-meter
magnetic stirrer and small stirring bar
two 50-mL burets
Note: If performing this experiment in the studio classroom use 25-mL burets.
2. A. Buffer Solution Preparations
1. All glassware must be clean. If the graduated cylinders and burets to be used are not
totally dry, rinse them first with three small portions of the solution to be used. Drain out
the rinsing solution each time as completely as you can. Then fill them with the
required solution.
2. Set up the burets with about 30mL of 0.100 M HCl in one and 30 mL of 0.100 M NaOH in
the other.
3. Prepare your first buffer by mixing 50.0 mL of 0.100 M NaOAc and 45.0 mL of 0.100 M
HOAc. Use graduated cylinders for these volume measurements. Mix well. Label this
Buffer ONE
4. Prepare your second buffer by mixing 50.0 mL of 0.100 M NaOH and 95.0 mL of 0.100 M
HOAc . Use graduated cylinders for these volume measurements. Mix well. Label this
Buffer TWO.
5. Measure and record the pH values of the two buffer solutions prepared.
B. Addition of Acid and Base to the Buffers
1. Place 30.0 mL of Buffer ONE into a clean dry 50-mL beaker. Add the magnetic stirring
bar and put the beaker on the magnetic stirrer. Turn the stirrer on and adjust it so that
it is stirring but not splashing. Add five drops of bromocresol green indicator to the
buffer. Insert the pH-meter probe so that the stirring bar does not hit it. Record the
color of the buffer, record its pH.
2. Arrange the buret so that you can add the 0.100 M HCl solution to the buffer and
measure the pH while the solution is continuously stirred.
3. The suggested volumes of HCl to be added are: 0.50, 1.00, 1.50, 2.25, 3.00, 3.50, 3.50,
2.00 mL. (8 additions of HCl for a total volume of 19.25 mL) The volume increments
which you actually add may differ from these values by up to 0.20 mL, but you must
have a record of the exact volumes which you added. Read the buret at the
beginning and after each addition. Obtain exact values by subtraction.
4. Record the color, pH value, and the total volume of HCl after each addition in your
laboratory notebook.
5. When you've completed your measurements you may discard the solution in the sink.
Don’t lose the magnetic stirring bar!
6. Repeat Steps1 to 4 using 0.100 M NaOH solution instead of the 0.100 M HCl solution.
3. C. Addition of Acid and Base to Deionized water
1. Pour 30.0 mL deionized water into a clean dry 50-mL beaker. Add five drops of
bromocresol green indicator. Measure the pH and note the color. Add the following
volume increments of 0.100 M HCl solution to the water with continual magnetic
stirring: 0.25 mL then three of 0.50 mL each, then three of 1.00mL each.
2. Record the color, pH value, and the total volume of HCl after each addition.
3. Discard the solution in the sink. Don’t lose the magnetic stirring bar!
4. Repeat Steps1 to 3 using 0.100 M NaOH solution instead of the 0.100 M HCl solution.
D. Addition of Acid and Base to Diluted Buffer
1. Pour 15.0 mL of Buffer TWO into a clean dry 50-mL beaker. Add five drops of
bromocresol green. Add 15.0 mL deionized water. Set the beaker on the magnetic
stirrer, stir, and measure the pH. Note the color.
2. Add the following increments of 0.100 M HCl solution with continuous stirring. Record
the pH values, color, and the total volume added after each increment. The
suggested increments are: 0.50 mL, then 6 portions 1.00 mL each, then 3 portions 1.50
mL each.
3. Discard the solution from the beaker in the sink. Don’t lose the magnetic stirring bar!
4. Repeat Steps1 to 3 using 0.100 M NaOH solution instead of 0.100 M HCl solution.
Clean-up:
1. Discard all solutions in the sink. Don’t lose the magnetic stirring bar!
2. Rinse the burets and pipets with deionized water before returning them.
3. Return the pH meters to their appropriate containers.
4. Sample Data Charts
Data must be recorded in your laboratory notebook.
Buffer and Their Properties
Table 1: Addition of acid, HCl, to Buffer ONE
Color pH obs Total volume
HCl added (mL)
mmols H+ added [H+] pH calc
Table 2: Addition of base, NaOH, to Buffer ONE
Color pH obs Total volume
NaOH added (mL)
mmols OH- added [H+] pH calc
5. Table 3: Addition of acid, HCl, to deionized water
Color pH obs Total volume HCl
added (mL)
mmols H+ added [H+] pH calc
Table 4: Addition of base, NaOH, to deionized water
Color pH obs Total volume NaOH
added (mL)
mmols OH- added [H+] pH calc
6. Table 5: Addition of acid, HCl, to Diluted Buffer TWO
Color pH obs Total volume
HCl added (mL)
mmols H+ added [H+] pH calc
Table 6: Addition of base, NaOH, to Diluted Buffer TWO
Color pH obs Total volume
NaOH added (mL)
mmols OH- added [H+] pH calc
7. Preparation Instructions for Properties of Buffers
4 sets of the following:
500mL bottle of 0.1 M acetic acid
500mL bottle of 0.1 M sodium acetate
200mL bottle 0.1 M HCl
200mL bottle 0.1 M NaOH
dropper bottle bromocresol green indicator
pH-meter (in studio classroom)
magnetic stirrer and small stirring bar (in studio classroom)
eight 25-mL burets (use short burets if doing this experiment in the studio classroom)