Variability arises in plant pathogens through various genetic mechanisms such as mutation, hybridization, and recombination. This variability allows pathogens to evolve new races or strains that can infect resistant host varieties and overcome plant resistance. The document discusses several mechanisms that generate variability in fungi, bacteria, and viruses, including mutation, transformation, transduction, conjugation, heterokaryosis, parasexualism, and recombination, which allow pathogens to adapt to new environments and hosts. Understanding pathogen variability is important for breeding disease-resistant crop varieties.
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
This ppt illustrates and describes the two bacterial diseases included in the BSc Hons Program Syllabys Core Course III or DSC 3- Citrus canker and angular leaf spot of cotton
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
This ppt illustrates and describes the two bacterial diseases included in the BSc Hons Program Syllabys Core Course III or DSC 3- Citrus canker and angular leaf spot of cotton
Effect of environment and nutrition on plant disease developmentparnavi kadam
BRIEF AND PRECISE POINTS ON PLANT DISEASE DEVELOPMENT. IT MOSTLY FOCUSES ON HOW THE FACTORS AFFECT THE MICROBES AND THEN THEIR MICROBIAL EFFECT ON DISEASE DEVELOPMENT.
In this slide you will get all the important information of epidemiology.
For more information you can see my youtube channel
https://www.youtube.com/channel/UCUsmJMc2xvL3O3UkDh8knrA
Effect of environment and nutrition on plant disease developmentparnavi kadam
BRIEF AND PRECISE POINTS ON PLANT DISEASE DEVELOPMENT. IT MOSTLY FOCUSES ON HOW THE FACTORS AFFECT THE MICROBES AND THEN THEIR MICROBIAL EFFECT ON DISEASE DEVELOPMENT.
In this slide you will get all the important information of epidemiology.
For more information you can see my youtube channel
https://www.youtube.com/channel/UCUsmJMc2xvL3O3UkDh8knrA
Introduction,In some fungi ,true sexual cycle comprising of nuclear fusion and meiosis is absent.
These fungi derive the benefits of sexuality through a cycle know as parasexuaL cycle.
First Reported by- Gudio Pontecorvo and J.A.Roper(1952)
Parasexual cycle was reported in
Aspergillus nidulans,the imperfect stage of Emericella nidulans.
Since then parasexual cycle has been discovered not only in several members of Deutromycetes but also in fungi belonging to Ascomycetes and Basidiomycetes.
DEFINETION - Parasexuality is defined as a cycle in which Plasmogamy, Karyogamy and Meiosis [Haploidization] take place in sequence but not at a specified time or at specified points in the life cycle of an organism.
Generally parasexual cycle occurs in those fungi in which true sexual cycle does not take place.
Parasexualcycle also know as Somatic recombination. PASEXUALITY ALSO REPORTED IN SOME ORGANISMS- Aspergillus nigar, Penicillium crysogenum, STEPS OF PARASEXUAL CYCLE - 1) ESTABLISHMENT OF HETEROKARYOSIS, 2) Formation of Heterozygous DIPLOIDS, 3) occasional mitotic crossing-over during multiplication of diploid nuclei, 4)occasional haplodization through aneuploidy , COMPARISION BETWEEN SEXUAL AND PARASEXUAL CYCLE, IMPORTANCE OF PARASEXUALITY, C0NCLUSION
Plant - Pathogen Interaction and Disease DevelopmentKK CHANDEL
Plant diseases are the result of infection by any living organisms that adversely affect the growth, development, physiological functioning and productivity of a plant, manifesting outwardly as visible symptoms.
Signal transduction in plant defence responsesrkravikirankt
Plant respond to the attack of diseases by triggering various bio-molecules insider their system to combat the infection and establishment of the pathogens. these response operate in specified pathways mediated by many enzymes starting from the infection site to the nucleus which together constitute the signal transduction pathway.
Functional Genomics of Plant Pathogen interactions in Wheat Rust PathosystemSenthil Natesan
Cereal rust fungi are pathogens of major importance to agriculture, threatening cereal production worldwide. Targeted breeding for resistance, based on information from fungal surveys and population structure analyses of virulence, has been effective. Nevertheless, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to determine the range of available resistance genes. The development of genomic resources for these fungi and their comparison has released a torrent of new ideas and approaches to use this information to assist pathologists and agriculture in general. The sequencing of gene transcripts and the analysis of proteins from haustoria has yielded candidate virulence factors among which could be defence-triggering avirulence genes. Genome-wide computational analyses, including genetic mapping and transcript analyses by RNA sequencing of many fungal isolates, will predict many more candidates (Bakkeren et al., 2012)
Dissecting the mechanisms of host-pathogen systems like wheat-rust, including pathogen counter-defenses will ensure a step ahead towards understanding current outcomes of interactions from a co-evolutionary point of view, and eventually move a step forward in building more durable strategies for management of diseases caused by fungi (Hadrami et al.,2012)
Two hydrothermal vent fields have been described at the ultra-slow spreading ridge of the Mid-Cayman Rise (MRC), including the world’s deepest (Piccard ~4985m) and the nearby Von Damm vent field (~2300m). Both vent fields support a localized high-biomass. The food web has chemoautotrophic bacteria at the base and includes bacterivorous shrimp as well as carnivores: shrimp and anemones.
The alvinocaridid shrimp Rimicaris hybisae is abundant at both vent fields and shows spatial variability in population structure. So far it has been considered bacterivorous. Large variations in tissue δ13C values remained largely unexplained, and it has been argued that δ13C values are not a good food web tracer in hydrothermal vent ecosystems.
We observed that shrimp tended to be either in dense aggregations on active chimneys, or more sparsely distributed and peripheral in (near) ambient temperatures. With the hypotheses that varying δ13C values show real differences in food sources and that shrimp in different locales might have different diets, we collected shrimp from both environments at the Von Damm site during an Ocean Exploration Trust Expedition with E/V Nautilus (NA034, 08/2013) and examined their gut contents.
Gut contents of all shrimp from dense aggregations consisted of white, amorphous material that resembled bacteria. Sparsely distributed shrimp (~1m from dense aggregations) had guts filled with fragments of crustacean exoskeleton, a mixture of bacteria-like material and crustacean exoskeleton, or bacteria-like material only.
We analyzed stable isotope compositions of the shrimp and their gut contents. Shrimp δ13C, δ15N and δ34S values reflect those of their gut contents +1 trophic level. Sparse shrimp have dramatically lower δ13C and δ34S values, and slightly elevated δ15N values, in comparison to dense shrimp. Sparse and dense R. hybisae clearly have different diets. Ongoing work is determining what exactly is this crustacean food source, whether diet changes occur during life history, and if this is linked to the molting cycle.
Presentation by Dr. Jim Guldin to support the Adaptive Silviculture for Climate Change (ASCC) J.W. Jones Ecological Research Center Workshop held January 12-14, 2016
1. What is pathogen variability?
2. Significance of pathogen Variability
3. Stages of variation
4. Mechanism of Variability in fungi
5. Characterization of variability among plant pathogens
The power point presentation includes information regarding various methods and concepts involved in fungi bacteria and virus with some suitable examples
the mechanisms of parasite evolution,
the factors that influence the rate and direction of evolution,
the implications of evolution for the control and management of parasitic diseases, and finally
the dynamic of Host-Parasite Coevolution.
SOMACLONAL VARIATION AND ITS SIGNIFICANCE.pptxVandana Yadav03
Somaclonal variations-introduction, history, source material for somaclonal variation, selection of somaclonal variation, kinds of variation, types of variation, causes of somaclonal variation, isolation of somaclonal variation, factors responsible for variation, application for somaclonal variation. Disadvantage.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
2. GENETICS AND VARIABILITY OF PLANT
PATHOGENS
• Dr. E.C. Stakman (U.S. Deptt. Of Agriculture)
defined need and use of variability in fungi
• Pathogen population mutates with time and
environmental conditions like stress
• In nature variability in organisms may be due
to:
HYBRIDIZATION MUTATION
3. • When progenies show variations in
characterstics from parents it is called as a
VARIANT.
• Physiological specialization: – with in the
species of a pathogen there exist certain
individuals that are morphologically similar
but differs w.r.t their physiology, biochemical
characters and pathogenicity and are
differentiated on the basis of their reaction on
certain host genera or cultivars.
4. • Physiological race: – a group of population
within a species which have ability of infecting
a particular genotype and do not differ in their
morphology but have physiological differences
such as a specific host or food type or
pathogenicity
• Variability: it is the property of an organism to
change its characters from one generation to
the other
5. • With in species or f.sp. There are further
subgroups of individual that infect different
varietes of the host– such subgroups are
called RACES/ STRAINS
• A sub-group within a species or race,
characterized by a common possesion of
single of few new characters and differ only in
few minor characters from parents are called
BIOTYPES
6. BREAKDOWN OF RESISTANCE:
• This term is used when a previously resistant
variety suddenly develops disease.
• It implies that the host has changed, that the
resistance mechanisms no longer work.
• New pathogen races have developed because
of selection pressure that was put on
population by the host resistance mechanism
7. Races are defined by ability to develop on specific host
genotypes (varieties, cultivars, hybrids, etc.).
Races are identified by ability to cause disease on members
of a set of 10 differential varieties that contain specific
resistance genes.
V1 V2 V3 V4 V5 V6 V7 V8 V9 V10 Race
x x x 14
x x x 27
x x 31
9. HYBRIDIZATION
• Mating of dissimilar strains or species of fungi
• 2 haploid nuclei (1N) with different genetic
material combine to form a diploid
nucleus(zygote)
• Eg. Basidiospores( haploid) from different races
often infect the same leaf or plant
Chances of dikaryotization high
10. HERTEROKARYOSIS
Hyphae or parts of hyphae contain nuclei,
which are genetically different, generally of
two different kinds. This condition is known as
heterokaryosis .
The phenomenon is commonly brought about
by hyphal anastomosis between mycelia of
two parental genotypes.
In Ascomycotina and Basidiomycotina
11. PARASEXUALISM
• First demonstrated by Pontecorvo (1956) in
Aspergillus nidulans.
Parasexualism is the process by which genetic
recombination can occur with in fungal
heterokaryon :
• Stage 1: heterokaryosis
• Stage 2: recombination
12. PARASEXUALISM
• Dissimilar nuclei to fuse and produce diploids
known as mitotic recombination.
• Recombination without sexual cycle
• There is no fine coordination between
plasmogamy, karyogamy and haplodisation
• This sequence of events has been described in
the parasexual cycle
13. PARASEXUALISM IMPORTANCE
• In rust fungi as P. graminis tritici, mitotic
recombination may represent a most
important method of generating new races
especially in countries such as India where
sexual stage of the fungus is rare due to
scarcity of the alternate host, the barberry.
• Common in deutromycotina
14. RECOMBINATION
• When two haploid nuclei (1N) containing
different gnentic maeterial unite to form diploid
(2N) nucleus (Zygote,) when under go meiotic
division produce new haploid .
• Recombination of genes occurs during meiotic
division of zygote as a result of cross over in
which part of chromatid of one chromosome of a
pair are expressed with that of the other
• Important in fungi Puccinia graminis.
15.
16. Cereal rust fungus variability
• Races of rust pathogen Puccinia graminis
differ from each other in shape and size of
uredospores but principle diffrence between
them is
• Preference for groups of host in different
members of graminae
Eg. Race tritici, secalis, avenae etc
17. MUTATION
Sudden heritable change in genetic material of an
organism
Mutation represent change in sequences of the
bases in DNA either by – substitution or by
deletion or addition
Mutations are spontaneous
May occur due to:
1. Improper cell division
2. Abnormalities during division
3. Physical radiations
4. Mutagenic chemicals
18. CYTOPLASMIC ADAPTATION
• Pathogens develop capicity to perform
biochemical reactions which were not present
in them earlier
• Can utilize protoplasm of unfavourable host.
• Adaptation to new cytoplasm
19. Three types of cytoplasmic
adaptatibility
I. Pathogen may aquire tolerance to toxic
materials
II. Utilization of new types of cytoplasm
III. Change in virulence
21. CONJUGATION
• Transfer of DNA from one bacterial cell to
another Donor cell (F+) transfers DNA to
recipient cell (F- )
• In this two compatible bacteria come in
contact and exchange the portion of plasmid
or chromosome through conjugation bridge or
pilus
28. VARIABILITY IN VIRUSES
Recombination
• May results from mixed infection of two
strains of the virus
• Occurs mostly during replication
Mutation – Results from nucleotide changes
in the coding regions due to addition or
deletion or replacement. – Ultimately leads to
functional changes in the genes.