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PHYSICAL CHEMISTRY
PAPER-I
NAME :- NIKITA .V. SINGH
COLLEGE-G.N.KHALSA COLLEGE
M.Sc. SEMESTER IV
ROLLNO-3
TOPIC-BIO-PHYSICAL CHEMISTRY.
CONTENTS
Complex Bio-molecules.
• Proteins.
• Lipids.
• Polysaccharides.
• Enzymes.
Introduction to Biomolecules
• Organic compounds present in living organism.
• Compounds oxygen, carbon, hydrogen, nitrogen.
Biomolecules
Organic Inorganic
• Biomolecules Micro molecules and Macromolecules
Proteins
• Proteins large biomolecules or macromolecules
consisting long chain of amino acids.
• most important functioning of cell.
• Polymers of amino acids.
• Proteins on hydrolysis yield amino acids.
• Synthesis controlled by DNA.
• Collagen animal protein.
• Rubisco plant protein.
Amino acids
• Two functional groups NH2 & COOH.
• Contain Carbon, Hydrogen, Oxygen, Nitrogen & some Sulphur.
• 20 amino acids present.
Peptide formation
• Amino acid are linked serially by peptide bonds formed between one
of amino acid and one of carboxylic acid .
• Chain having two amino acids linked by peptide bond is called
dipeptide bond.
Structure of
protein
4 basic structural levels are assigned to proteins-
primary, secondary, tertiary and quaternary
Primary
• The primary structure refers to number of amino
acids in the polypeptide chain and the location
of disulphide chain.
• The N- terminal amino acid written on left side
whereas the C- terminal amino acid is written on
the right side.
Secondary
The folding of linear chain specific coiled structure
secondary structure.
Tertiary
The helical polypeptide may fold itself and assume
a complex but specific form – spherical, rod like.
Quaternary
Proteins are said to be quaternary in structure if
they have 2 or more polypeptide chains .
Haemoglobin is best example.
Lipids
• Insoluble in water Lipids Soluble in organic solvents.
• Important component of living cell.
Lipids
Simple Complex Derived
Fats and
oils
Waxes
Phospholipids
Glycolipids
Lipoproteins
Steroids
Terpenes
Simple lipids
• Fatty acid ester of different alcohols and
carries no other substances.
• Belong to heterogenous class .
Made up C,H,O but O is
less.
Esters of fatty acids with
glycerol.
Neutral or true
fats
Made up of two components
Glycerol and fatty acids.
• It is an simple lipid which are long chain saturated fatty acids a long
chain saturated alcohol of high mol weight other than glycerol.
• Example :
1) Bees wax : secretion of abdominal glands of worker honey bees.
2) Wool fat : secretion of cutaneous glands and obtained from wool of
sheep.
3) Plant wax : coating formed on the plants organs to prevent
transpiration.
4) Paraffin wax : A translucent waxy substance obtained from
petroleum.
Waxes
Complex lipids
They are derivative of simple lipids having
additional group like phosphate, N2-base,
protein etc
Phospholipids Glycolipid Lipoprotein
• They are made up of a molecule of glycerol or other alcohol having
1) A phos group at 1 of its –OH groups.
2) 2 fatty acid molecules at other 2-OH groups.
3) A nitrogen containing base attached to phos group.
• A phospholipid molecule has a hydrophobic tail (fatty acids) and a
hydrophilic head (phos group).
Phospholipids
Glycolipid
They are components of cell
membranes, particularly myelin sheath
and chloroplast membranes .
CEREBROSIDE are the most simplest
form of glycolipids.
• They contain lipids and proteins in their molecules .
• They are main constituent of membranes.
• They are found in milk and egg yolk.
Lipids are transported in blood and lymph as lipoproteins.
• 5 types of lipoproteins :
1. Chylomicrons.
2. VLDL
3. LDL
4. HDL
5. Free fatty acid albumin complex.
Lipoprotein
Derived lipids
They are derivatives obtained on the hydrolysis of the
simple and complex lipids
Steroids Terpenes
References
• Textbook of Biophysical Chemistry, U.N.Dash, Published by Rajiv Beri
for Macmillan India Ltd., 2006, Page No. 52 – 64.
• Chemistry of Biomolecules, S.P.Bhutani, Published by Ane Books Pvt.
Ltd., 2009, Page No. 134 – 142.
• Biophysics principles and techniques, M.A.Subramanian, MJP
Publishers, Page No. 26 – 41.
THANKYOU

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Paper 1

  • 1. PHYSICAL CHEMISTRY PAPER-I NAME :- NIKITA .V. SINGH COLLEGE-G.N.KHALSA COLLEGE M.Sc. SEMESTER IV ROLLNO-3 TOPIC-BIO-PHYSICAL CHEMISTRY.
  • 2. CONTENTS Complex Bio-molecules. • Proteins. • Lipids. • Polysaccharides. • Enzymes.
  • 3. Introduction to Biomolecules • Organic compounds present in living organism. • Compounds oxygen, carbon, hydrogen, nitrogen. Biomolecules Organic Inorganic • Biomolecules Micro molecules and Macromolecules
  • 4. Proteins • Proteins large biomolecules or macromolecules consisting long chain of amino acids. • most important functioning of cell. • Polymers of amino acids. • Proteins on hydrolysis yield amino acids. • Synthesis controlled by DNA. • Collagen animal protein. • Rubisco plant protein.
  • 5. Amino acids • Two functional groups NH2 & COOH. • Contain Carbon, Hydrogen, Oxygen, Nitrogen & some Sulphur. • 20 amino acids present.
  • 6. Peptide formation • Amino acid are linked serially by peptide bonds formed between one of amino acid and one of carboxylic acid . • Chain having two amino acids linked by peptide bond is called dipeptide bond.
  • 7. Structure of protein 4 basic structural levels are assigned to proteins- primary, secondary, tertiary and quaternary Primary • The primary structure refers to number of amino acids in the polypeptide chain and the location of disulphide chain. • The N- terminal amino acid written on left side whereas the C- terminal amino acid is written on the right side.
  • 8. Secondary The folding of linear chain specific coiled structure secondary structure.
  • 9. Tertiary The helical polypeptide may fold itself and assume a complex but specific form – spherical, rod like. Quaternary Proteins are said to be quaternary in structure if they have 2 or more polypeptide chains . Haemoglobin is best example.
  • 10. Lipids • Insoluble in water Lipids Soluble in organic solvents. • Important component of living cell. Lipids Simple Complex Derived Fats and oils Waxes Phospholipids Glycolipids Lipoproteins Steroids Terpenes
  • 11. Simple lipids • Fatty acid ester of different alcohols and carries no other substances. • Belong to heterogenous class . Made up C,H,O but O is less. Esters of fatty acids with glycerol. Neutral or true fats Made up of two components Glycerol and fatty acids.
  • 12. • It is an simple lipid which are long chain saturated fatty acids a long chain saturated alcohol of high mol weight other than glycerol. • Example : 1) Bees wax : secretion of abdominal glands of worker honey bees. 2) Wool fat : secretion of cutaneous glands and obtained from wool of sheep. 3) Plant wax : coating formed on the plants organs to prevent transpiration. 4) Paraffin wax : A translucent waxy substance obtained from petroleum. Waxes
  • 13. Complex lipids They are derivative of simple lipids having additional group like phosphate, N2-base, protein etc Phospholipids Glycolipid Lipoprotein
  • 14. • They are made up of a molecule of glycerol or other alcohol having 1) A phos group at 1 of its –OH groups. 2) 2 fatty acid molecules at other 2-OH groups. 3) A nitrogen containing base attached to phos group. • A phospholipid molecule has a hydrophobic tail (fatty acids) and a hydrophilic head (phos group). Phospholipids
  • 15. Glycolipid They are components of cell membranes, particularly myelin sheath and chloroplast membranes . CEREBROSIDE are the most simplest form of glycolipids.
  • 16. • They contain lipids and proteins in their molecules . • They are main constituent of membranes. • They are found in milk and egg yolk. Lipids are transported in blood and lymph as lipoproteins. • 5 types of lipoproteins : 1. Chylomicrons. 2. VLDL 3. LDL 4. HDL 5. Free fatty acid albumin complex. Lipoprotein
  • 17. Derived lipids They are derivatives obtained on the hydrolysis of the simple and complex lipids Steroids Terpenes
  • 18. References • Textbook of Biophysical Chemistry, U.N.Dash, Published by Rajiv Beri for Macmillan India Ltd., 2006, Page No. 52 – 64. • Chemistry of Biomolecules, S.P.Bhutani, Published by Ane Books Pvt. Ltd., 2009, Page No. 134 – 142. • Biophysics principles and techniques, M.A.Subramanian, MJP Publishers, Page No. 26 – 41.

Editor's Notes

  1. R can be h in glycine ch3 in alanine hydroxymethyl in serine