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Biological nitrogen fixation (BNF) can be defined as the conversion of atmospheric dinitrogen (N2) to ammonia (NH3) under the combined action of biological and chemical activities
this lesson explains the basic biochemical/biological process behind Nitrogen fixation by microorganism which could be symbiotic or non symbiotic/free living in mechanism.
Biological nitrogen fixation (BNF) can be defined as the conversion of atmospheric dinitrogen (N2) to ammonia (NH3) under the combined action of biological and chemical activities
this lesson explains the basic biochemical/biological process behind Nitrogen fixation by microorganism which could be symbiotic or non symbiotic/free living in mechanism.
biological nitrogen fixation, which is carried out by diazotrophs, has been dealt with in this slideshare. it involves the mechanism involved and various factors involved therein.
This is a comprehensive account of the nitrogen cycle in terrestrial environments. The nitrogen cycle is responsible for the circulation of nitrogen between inorganic and organic components of the environment.
Biological Nitrogen Fixation
Contents:
Introduction
Methods for measuring N2 fixation
1. Ntrogen balance method
2. Nitrogen difference method
3. Ureides method
4.〖𝟏𝟓〗_𝑵 isotope techniques
5. Acetylene reduction assay
6. Hydrogen evolution method
Introduction
N2 gas are found 78.084%on atmosphere of earth.
Nitrogen is an essential element for plant growth and development and a key issue of agriculture.
N2 are found in molecular N2 (𝑵 ≡ 𝑵) form in soil.
Dinitrogen is more stable, so we need of nitrogen fixation.
Most studies indicate that nitrogen fertilizers contribute to resolving the challenge the world is facing, feeding the human population.
The Green revolution was accompanied by an enormous increase in the application of nitrogen fertilizer.
Nitrogen fixation is a process by which nitrogen of the Earth's atmosphere is converted into ammonia (NH3), nitrogen salts or other molecules available to living organisms.
Biological Nitrogen Fixation(BNF) is known to be a sustain agriculture and increase soil fertility.
Research on microorganisms and plants able to fix nitrogen contributes largely to the production of bio fertilizers.
Thus it is important to ensure that BNF research and development will take into account the needs of farmers in the developing countries mainly.
Role of nitrogen in Plant
Sources of Nitrogen
Why measure 𝑵_𝟐 fixation?
Ecological consideration require an understanding of the relative contribution of 𝑵_𝟐 fixing components to the N-cycle.
Measurement of 𝑁_2 fixation enable an investigator to evaluate the ability of indigenous Rhizobium spp. to effectively nodulate newly introduced legumes.
Development of sustainable farming systems.
Understanding of the amount of 𝑵_𝟐fixed by legumes as influenced by soil management or cultural practices allows development of efficient agricultural and agroforesty production systems.
The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into various chemical forms as it circulates among the atmosphere and terrestrial and marine ecosystems. The conversion of nitrogen can be carried out through both biological and physical processes. Important processes in the nitrogen cycle include fixation, ammonification, nitrification, and denitrification. The majority of Earth's atmosphere (78%) is nitrogen, making it the largest pool of nitrogen. However, atmospheric nitrogen has limited availability for biological use, leading to a scarcity of usable nitrogen in many types of ecosystems. The nitrogen cycle is of particular interest to ecologists because nitrogen availability can affect the rate of key ecosystem processes, including primary production and decomposition. Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitrogen cycle.
CS_701_Nitrate Assimilation by arnold_damasoAr R Ventura
Nitrate assimilation is the formation of organic nitrogen compounds like amino acids from inorganic nitrogen compounds present in the environment. Organisms like plants, fungi and certain bacteria that cannot fix nitrogen gas (N2) depend on the ability to assimilate nitrate or ammonia for their needs.
Plants like castor reduce a lot of nitrate in the root itself, and excrete the resulting base. Some of the base produced in the shoots is transported to the roots as salts of organic acids while a small amount of the carboxylates are just stored in the shoot itself. However, about 99% of the organic nitrogen in the biosphere is derived from the assimilation of nitrate. NH4+ is formed as an end product of the degradation of organic matter, primarily by the metabolism of animals and bacteria, and is oxidized to nitrate again by nitrifying bacteria in the soil. Thus a continuous cycle exists between the nitrate in the soil and the organic nitrogen in the plants growing on it. While nearly all the ammonia in the root is usually incorporated into amino acids at the root itself, plants may transport significant amounts of ammonium ions in the xylem to be fixed in the shoots. This may help avoid the transport of organic compounds down to the roots just to carry the nitrogen back as amino acids.
biological nitrogen fixation, which is carried out by diazotrophs, has been dealt with in this slideshare. it involves the mechanism involved and various factors involved therein.
This is a comprehensive account of the nitrogen cycle in terrestrial environments. The nitrogen cycle is responsible for the circulation of nitrogen between inorganic and organic components of the environment.
Biological Nitrogen Fixation
Contents:
Introduction
Methods for measuring N2 fixation
1. Ntrogen balance method
2. Nitrogen difference method
3. Ureides method
4.〖𝟏𝟓〗_𝑵 isotope techniques
5. Acetylene reduction assay
6. Hydrogen evolution method
Introduction
N2 gas are found 78.084%on atmosphere of earth.
Nitrogen is an essential element for plant growth and development and a key issue of agriculture.
N2 are found in molecular N2 (𝑵 ≡ 𝑵) form in soil.
Dinitrogen is more stable, so we need of nitrogen fixation.
Most studies indicate that nitrogen fertilizers contribute to resolving the challenge the world is facing, feeding the human population.
The Green revolution was accompanied by an enormous increase in the application of nitrogen fertilizer.
Nitrogen fixation is a process by which nitrogen of the Earth's atmosphere is converted into ammonia (NH3), nitrogen salts or other molecules available to living organisms.
Biological Nitrogen Fixation(BNF) is known to be a sustain agriculture and increase soil fertility.
Research on microorganisms and plants able to fix nitrogen contributes largely to the production of bio fertilizers.
Thus it is important to ensure that BNF research and development will take into account the needs of farmers in the developing countries mainly.
Role of nitrogen in Plant
Sources of Nitrogen
Why measure 𝑵_𝟐 fixation?
Ecological consideration require an understanding of the relative contribution of 𝑵_𝟐 fixing components to the N-cycle.
Measurement of 𝑁_2 fixation enable an investigator to evaluate the ability of indigenous Rhizobium spp. to effectively nodulate newly introduced legumes.
Development of sustainable farming systems.
Understanding of the amount of 𝑵_𝟐fixed by legumes as influenced by soil management or cultural practices allows development of efficient agricultural and agroforesty production systems.
The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into various chemical forms as it circulates among the atmosphere and terrestrial and marine ecosystems. The conversion of nitrogen can be carried out through both biological and physical processes. Important processes in the nitrogen cycle include fixation, ammonification, nitrification, and denitrification. The majority of Earth's atmosphere (78%) is nitrogen, making it the largest pool of nitrogen. However, atmospheric nitrogen has limited availability for biological use, leading to a scarcity of usable nitrogen in many types of ecosystems. The nitrogen cycle is of particular interest to ecologists because nitrogen availability can affect the rate of key ecosystem processes, including primary production and decomposition. Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitrogen cycle.
CS_701_Nitrate Assimilation by arnold_damasoAr R Ventura
Nitrate assimilation is the formation of organic nitrogen compounds like amino acids from inorganic nitrogen compounds present in the environment. Organisms like plants, fungi and certain bacteria that cannot fix nitrogen gas (N2) depend on the ability to assimilate nitrate or ammonia for their needs.
Plants like castor reduce a lot of nitrate in the root itself, and excrete the resulting base. Some of the base produced in the shoots is transported to the roots as salts of organic acids while a small amount of the carboxylates are just stored in the shoot itself. However, about 99% of the organic nitrogen in the biosphere is derived from the assimilation of nitrate. NH4+ is formed as an end product of the degradation of organic matter, primarily by the metabolism of animals and bacteria, and is oxidized to nitrate again by nitrifying bacteria in the soil. Thus a continuous cycle exists between the nitrate in the soil and the organic nitrogen in the plants growing on it. While nearly all the ammonia in the root is usually incorporated into amino acids at the root itself, plants may transport significant amounts of ammonium ions in the xylem to be fixed in the shoots. This may help avoid the transport of organic compounds down to the roots just to carry the nitrogen back as amino acids.
The Nitrogen cycle is defined as the biogeochemical cycle process that involves transforming the inert nitrogen that is available in the atmosphere, into a more usable or conventional form, that can be actively used by plants, and various living organisms. Enroll now at Tutoroot.
Nitrogen is important element of life. In importance it comes only next to carbon, hydrogen, and oxygen. The composition of protein, nucleic acid, growth hormones, and vitamins requires Nitrogen. Leaves consist of about 1 to 15% nitrogen of their dry weight but lesser % in another vegetative organ.
• The N2 is present in the atmosphere, in the form of gas. It is about 78%.
• Green plants unable to use this N2 directly in their metabolism. Only some micro-organism can convert this N2 gas directly into organic form.
• The N2 present in the soil is called soil nitrogen. The plants growing in the soil, mainly utilize the soil N2 for their metabolic requirements.
• In the soil the nitrogen is present in the form of nitrate nitrogen (NO3, NO2), ammonia nitrogen (ammonia, ammonium salt), organic nitrogen and molecular nitrogen (N2).
• The converging of the free nitrogen, by natural or physical process is called nitrogen fixation… when any biological system is involved in this process, then it is called as biological nitrogen fixation……
La desnitrificación es un proceso metabólico que usa el nitrato como receptor terminal de electrones en condiciones anaerobias (ausencia de oxígeno) principalmente, conduciendo finalmente a nitrógeno molecular (gas, N2). La realizan exclusivamente ciertos microorganismos, entre los que destacan Alcaligenes, Paracoccus, Pseudomonas, Thiobacillus, Rhizobium, Thiosphaera, entre otros.Algunas bacterias desnitrificantes son quimiolitoautótrofas y pueden oxidar compuestos inorgánicos de azufre como sulfhídrico (H2S), azufre elemental (S0), tiosulfato(S2O32-) o sulfito(SO32-) anaeróbicamente a expensas de la reducción del nitrato.7 Entre ellas, autótrofos obligados que crezcan a pHs neutros tan solo se conocen dos: Thiobacillus denitrificans y Thiomicrospira denitrificans8 y pueden llevar a cabo la sulfoxidación en condiciones aeróbicas o anóxicas. Recientemente se ha aislado Thioalkalivibrio denitrificans, un autótrofo, oxidador de azufre, capaz de crecer anaeróbicamente usando nitrito como aceptor de electrones a pH básico9
Las ventajas de este proceso respecto a la heterotrofía son varias. Para el tratamiento de aguas residuales, evita tener que añadir materia orgánica, reduciéndose así los costes, y para tratamiento de aguas potables, evita carbono residual en el efluente, ya que reduce el riesgo de sobrecrecimiento en los sistemas a tratar y de desinfección de la zona por los productos producidos debido a que los organismos autotrófos crecen más despacio y producen menos biomasa, con la consiguiente formación de menos productos celulares.10 Además los organismos autótrofos están mejor adaptados para el tratamiento de aguas subterráneas porque crecen a bajas concentraciones de compuestos orgánicos biodegradables. También posee un gran interés comercial y desde el punto de vista de la biotecnología ambiental puesto que es uno de los pocos ejemplos en los que puede oxidarse biológicamente compuestos reducidos del azufre (sulfoxidación) en ausencia de oxígeno elemental. Pero la principal ventaja de este proceso es la aparición de la desnitrificación acoplada a la oxidación de compuestos reducidos del azufre, combinando la eliminación simultánea de dos tipos de contaminantes, los nitratos y los compuestos reducidos del azufre (ecuación 2), teniendo así gran interés por sus aplicaciones biotecnológicas.
Principales parámetros que afectan la desnitrificación
Temperatura
De acuerdo con la literatura, los microorganismos desnitrificantes presentan actividad entre 5 y 75 °C.11 Se ha visto que no existe un cambio significativo en la actividad desnitrificante entre 20 y 30 °C con consorcios provenientes de plantas de tratamiento de aguas residuales.12
pH
El pH óptimo para la desnitrificación se encuentra entre 7 y 8.413 A valores de pH debajo de 6, se inhibe la enzima óxido nitroso reductasa y se acumula óxido nitroso.1411 En la revisión de Cervantes-Carrillo et al. (2000)13 se reporta que en cultivos continuos no se observa ninguna acumulación
Roles of microbes in nitrogen cycle aritriyaaritriyajana
There are many presentation on nitrogen cycle.but in my case i have to make a ppt on microbes role in nitrogen cycle.so i made it.and then upload it if anyone get help from it i will be pleased. Aritriya Jana(F.F.Sc)
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
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http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
1. THE WORLD OF NITROGEN (N 2 ) NITROGEN FIXING NITROGEN CYCLE FAQ ABOUT (N 2 ) www.sciencetutors.zoomshare.com
2.
3. YOU SHOULD KNOW More than half of nitrogen fixing occurs through natural processes. However only 30% of fixing is accounted for by the Haber process.
4.
5. FERTLISERS These are chemicals used to replenish soil nutrients that have been removed by growing crops on farm land and to improve the fertility of the soil www.sciencetutors.zoomshare.com
6.
7. TASK TWO Two science students Patrick and Mark read an article about how to help farmers in less developed countries. Richer developed countries send synthetic fertilizers to help these farmers grow more food and boost food supply. Patrick advocates organic farming. He says it is better to use manure as synthetic fertilisers will harm the soil structure. Suggest reasons why it is not practical (technically feasible) to send manure to developing countries www.sciencetutors.zoomshare.com
8. NITROGEN FIXING Is a natural process (either biological or abiotic) by which Nitrogen (N 2 ) is made into compounds. In simple term(s), it is how nitrogen (N 2 ) gets "fixed" by bacteria to become available for plant use. www.sciencetutors.zoomshare.com
10. What is nitrogen cycle? How have humans interfered with the nitrogen cycle? www.sciencetutors.zoomshare.com
11.
12. NITROGEN FIXATION DIAGRAM Nitrogen Fixation The nitrogen molecule (N2) is quite inert. To break it apart so that its atoms can combine with other atoms requires the input of substantial amounts of energy. Three processes are responsible for most of the nitrogen fixation in the biosphere: atmospheric fixation by lightning biological fixation by certain microbes — alone or in a symbiotic relationship with plants industrial fixation
13. DENITRIFICATION Denitrification-Replenishing Nitrogen in the Atmosphere Four processes participate in the cycling of nitrogen through the biosphere: 1.nitrogen fixation , 2.Nitrification and 3.Decay The three processes above remove nitrogen from the atmosphere and pass it through ecosystems.
14. Denitrification Denitrification reduces nitrates to nitrogen gas, thus replenishing the atmosphere. Once again, bacteria are the agents. They live deep in soil and in aquatic sediments where conditions are anaerobic. They use nitrates as an alternative to oxygen for the final electron acceptor in their respiration. Thus they close the nitrogen cycle.
15. Human Impact On Nitrogen Cycle The activities of humans have severely altered the nitrogen cycle. Can you suggest/write down how we have influenced the nitrogen cycle
16. Fossil Fuel Increased deposition of nitrogen from atmospheric sources because of fossil fuel combustion and forest burning. Both of these processes release a variety of solid forms of nitrogen through combustion.
17. Pop quiz: How have humans interfered with the nitrogen cycle? A. Dumping of untreated sewage and urban runoff B. Burning fossil fuels and wood C. The use of inorganic fertilizers D. Over harvesting of legumes and nitrogen-rich mines E. All of the above
18. Nitrogen Overload When we cause nitrogen overload in an ecosystem, there are many drastic effects. Dumping of raw sewage contains nitrogenous wastes, along with urban runoff. When large amounts of nitrogen collect in a water body, eutrophication can result. This is an accumulation of excess nutrients which causes an algae bloom.
19. Burning Of Fossil Fuels Burning fossil fuels and wood contributes to a large amount of nitric oxide in the atmosphere. Nitric oxide can combine with oxygen gas to for nitrogen dioxide, which reacts with water vapour to form a strong acid (nitric acid). This can precipitate out of the atmosphere in the form of the deadly acid rain
20. ACID RAIN FROM FOSSIL FUELS The acid can damage trees and kill fish. Over mining nitrogen also alter an ecosystem.