This document discusses intrinsic in situ bioremediation. It explains that intrinsic bioremediation uses microorganisms already present in the environment to degrade contaminants, requiring no human intervention and being the cheapest form of bioremediation. Intrinsic bioremediation is tested at the lab and field levels before use to assess the microorganisms' ability to metabolize contaminants. Factors like moisture, pH, temperature, nutrients, electron acceptors, and toxin concentration affect the rate of intrinsic bioremediation. In situ bioremediation cleans up contaminated sites directly where pollution occurred, with options like biostimulation or bioaugmentation. It has advantages of being cost-effective with minimal exposure but

1. INTRINSIC IN SITU
BIOREMEDIATION
P . Pandiyammal @ Subathra
18UT12
III B.sc Biotechnology

2. BIOREMEDIATION
 Bioremediation is the use of living organisms, primarily
microorganisms, for the degradation of hazardous
chemicals in soil sediments, water, or other
contaminated materials into less toxic forms
 Microorganisms metabolize the chemicals to produce
carbon dioxide or methane, water and biomass
 Enzymatically transformed to metabolites that are less
toxic or innocuous
 In some instances, the metabolites formed are more toxic
than the parent compound. For example,
perchloroethylene and trichloroethylene may degrade
to vinyl chloride.

4. IN SITU BIOREMEDIATION:
 In situ bioremediation involves a direct approach for the
microbial degradation of pollution.
 In situ bioremediation is when the contaminated site is
cleaned up exactly where it occurred.
 Biostimulation : Addition of adequate quantities of
nutrients at the site promote microbial growth is done.
 When microorganisms are imported to a contaminated
site to enhance degradation, the process is called as Bio-
augmentation
 There is no need to excavate or remove soils or water in
order to complete remediation.

6. INTRINSIC BIOREMEDIATION
 Intrinsic bioremediation uses micro organisms
already present in the environment to biodegrade
harmful contaminant.
 There is no human intervension involved.
 Most commonly used
 The cheapest means of bioremediation available

7.  The intrinsic that is inherent capacity of microorganisms
to metabolize the contaminants should be tested at the
laboratory and field levels before use for intrinsic
bioremediation. Through site monitoring programmes
progress of intrinsic bioremediation should be recorded
time to time.
 The conditions of site that favour intrinsic
bioremediation are ground water flow throughout the
year, carbonate minerals to buffer acidity produced
during biodegradation, supply of electron acceptors and
nutrients for microbial growth and absence of toxic
compounds.
 Bioremediation of waste mixture containing metals such
as Hg, Pb, and cyanide at toxic concentration can create
problem

8. FACTORS EFFECTING RATE OF
INTRINSIC BIOREMEDIATION
 Moisture in soil
 Ph
 Temperature
 Soil nutirents
 Presence of electron acceptors
 Toxin concentration

9. ADVANTAGES OF IN SITU
BIOREMEDIATION
 Cost effective, with minimal exposure to public or
site personnel
 Sites of bioremediation remain minimally disrupted
DISADVANTAGES OF IN SITU
BIOREMEDIATION
 Very time consuming process
 Sites are directly exposed to environmental factors
such as temperature, oxygen supply etc.
 Microbial degrading ability varies seasonally

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