Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Phototrophy, chemotrophy and autotrophy in prokaryotesRahul Kunwar Singh
This document provides information about the microbial physiology course SLS/MIC/C007. The course covers microbial growth, metabolism, and cell structure over 5 units: 1) Phototrophy and Chemotrophy, 2) Respiration, 3) Nitrogen and Sulfur Metabolism, 4) Transport and Communication, and 5) Stress Response. Key topics include photosynthesis, chemolithotrophy utilizing hydrogen, sulfur, and iron oxidation, and carbon fixation pathways such as the Calvin cycle and reverse citric acid cycle that allow autotrophic growth.
Transduction is the transfer of genes between bacteria through bacteriophages. It was discovered in 1952 by Norton Zinder and Joshua Lederberg through experiments showing genetic exchange between Salmonella strains. Transduction occurs through either the lytic or lysogenic cycle of a bacteriophage. There are two main types of transduction: generalized, where any bacterial gene can be transferred, and specialized, where only certain genes are transferred. Transduction contributes to bacterial evolution and allows for the study of gene linkage.
1) The document discusses the process of natural transformation in bacteria, where DNA is transferred from one bacterial cell to another without direct contact.
2) It describes how competent bacterial cells can take up extracellular DNA released from lysed donor cells, and how the DNA can recombine into the recipient cell's genome.
3) The document compares natural transformation between gram-positive bacteria like Streptococcus pneumoniae and gram-negative bacteria like Haemophilus influenzae, noting differences in how DNA is taken up and the role of specific DNA sequences.
The document discusses various methods for improving microbial strains, including mutation, recombination, and recombinant DNA technology. Mutation methods include inducing mutations using mutagenic agents or site-directed mutagenesis to create specific changes. Recombination techniques involve combining genetic material between strains, such as transformation, transduction, conjugation, and protoplast fusion. Recombinant DNA technology allows introducing new genes to modify metabolic activities or produce recombinant proteins. The goal of strain improvement is to enhance microbial productivity, substrate utilization, or other desirable traits for industrial applications.
Basic Energy Yielding Mechanism of Chemoautotrophic & Photoautotrophic BacteriaGayatri R. Kachh
- The document discusses autotrophs and how they obtain energy and fix carbon. It focuses on two types of autotrophs: chemoautotrophs and phototrophs.
- Chemoautotrophs obtain energy through the oxidation of inorganic compounds like hydrogen sulfide or ferrous iron. This generates a proton gradient used to produce ATP via electron transport chains. One example is Thiobacillus ferrooxidans.
- Phototrophs use light energy to produce ATP through photophosphorylation. They contain light-harvesting pigments like chlorophyll and convert light to chemical energy. There are two types of light reactions: oxygenic and anoxygenic.
Methane can be produced by methanogenic bacteria that live in oxygen-free environments like mud, sewage, and the digestive systems of animals. These bacteria play an important role in the breakdown of organic matter by removing hydrogen gas and producing methane. Methane can also be oxidized by different types of bacteria that use it as an energy source, breaking it down into carbon dioxide and water through a process that requires oxygen. While bacterial methane production is important ecologically, current energy sources are typically more economical than biogas from bacterial sources.
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Phototrophy, chemotrophy and autotrophy in prokaryotesRahul Kunwar Singh
This document provides information about the microbial physiology course SLS/MIC/C007. The course covers microbial growth, metabolism, and cell structure over 5 units: 1) Phototrophy and Chemotrophy, 2) Respiration, 3) Nitrogen and Sulfur Metabolism, 4) Transport and Communication, and 5) Stress Response. Key topics include photosynthesis, chemolithotrophy utilizing hydrogen, sulfur, and iron oxidation, and carbon fixation pathways such as the Calvin cycle and reverse citric acid cycle that allow autotrophic growth.
Transduction is the transfer of genes between bacteria through bacteriophages. It was discovered in 1952 by Norton Zinder and Joshua Lederberg through experiments showing genetic exchange between Salmonella strains. Transduction occurs through either the lytic or lysogenic cycle of a bacteriophage. There are two main types of transduction: generalized, where any bacterial gene can be transferred, and specialized, where only certain genes are transferred. Transduction contributes to bacterial evolution and allows for the study of gene linkage.
1) The document discusses the process of natural transformation in bacteria, where DNA is transferred from one bacterial cell to another without direct contact.
2) It describes how competent bacterial cells can take up extracellular DNA released from lysed donor cells, and how the DNA can recombine into the recipient cell's genome.
3) The document compares natural transformation between gram-positive bacteria like Streptococcus pneumoniae and gram-negative bacteria like Haemophilus influenzae, noting differences in how DNA is taken up and the role of specific DNA sequences.
The document discusses various methods for improving microbial strains, including mutation, recombination, and recombinant DNA technology. Mutation methods include inducing mutations using mutagenic agents or site-directed mutagenesis to create specific changes. Recombination techniques involve combining genetic material between strains, such as transformation, transduction, conjugation, and protoplast fusion. Recombinant DNA technology allows introducing new genes to modify metabolic activities or produce recombinant proteins. The goal of strain improvement is to enhance microbial productivity, substrate utilization, or other desirable traits for industrial applications.
Basic Energy Yielding Mechanism of Chemoautotrophic & Photoautotrophic BacteriaGayatri R. Kachh
- The document discusses autotrophs and how they obtain energy and fix carbon. It focuses on two types of autotrophs: chemoautotrophs and phototrophs.
- Chemoautotrophs obtain energy through the oxidation of inorganic compounds like hydrogen sulfide or ferrous iron. This generates a proton gradient used to produce ATP via electron transport chains. One example is Thiobacillus ferrooxidans.
- Phototrophs use light energy to produce ATP through photophosphorylation. They contain light-harvesting pigments like chlorophyll and convert light to chemical energy. There are two types of light reactions: oxygenic and anoxygenic.
Methane can be produced by methanogenic bacteria that live in oxygen-free environments like mud, sewage, and the digestive systems of animals. These bacteria play an important role in the breakdown of organic matter by removing hydrogen gas and producing methane. Methane can also be oxidized by different types of bacteria that use it as an energy source, breaking it down into carbon dioxide and water through a process that requires oxygen. While bacterial methane production is important ecologically, current energy sources are typically more economical than biogas from bacterial sources.
Endospores are dormant structures produced by certain bacteria, such as Bacillus and Clostridium, that allow them to survive unfavorable environmental conditions. Endospores have a layered structure that makes them highly resistant to heat, radiation, disinfectants and other stresses. When conditions improve, endospores can activate, germinate and grow out into normal vegetative bacterial cells through a process of reactivation. The ability of endospores to survive harsh conditions makes some bacteria dangerous pathogens and able to contaminate areas after being thought sterilized.
nitrate and sulfate reduction ; methanogenesis and acetogenesisjyoti arora
this powerpoint includes the following topics in brief:
1. Nitrate assimilatory reduction
2. Sulfate assimilatory reduction
3. Methanogenesis
4. Acetogenesis
This document summarizes the microbial production of two important antibiotics - penicillin and streptomycin. It describes that penicillin is produced by fermentation of Penicillium chrysogenum in a nutrient medium, and involves inoculum preparation, fermentation process under controlled conditions, and downstream recovery and purification of penicillin. Streptomycin is derived from Streptomyces griseus and its fermentation in Hockenhull medium occurs in three phases before recovery of the antibiotic. Both antibiotics have important medical uses for treating bacterial infections.
1. Enzyme activity can be regulated through several mechanisms including allosteric regulation, feedback inhibition, proenzymes, and protein modification.
2. Allosteric enzymes have effector molecules that bind and induce a conformational change that increases or decreases enzyme activity. Feedback inhibition occurs when a metabolic end product inhibits an earlier enzyme.
3. Proenzymes are inactive precursors that are activated by proteolytic cleavage. Protein modification like phosphorylation can also regulate enzymes by changing their structure.
Gluconeogenesis is the metabolic pathway by which glucose is synthesized from non-carbohydrate materials to maintain blood glucose levels during periods without food intake. It takes place primarily in the liver and involves bypasses of three irreversible steps in glycolysis. Precursors like lactate, glycerol, and certain amino acids are converted to pyruvate and then glucose. The pathway requires energy in the form of 6 ATP molecules to synthesize one glucose molecule from two pyruvate. Gluconeogenesis is important for supplying glucose to tissues like the brain and helps maintain normal blood sugar through processes like the Cori cycle.
Difference between prokaryotic & eukaryotic DNA replicationJacksonchary
Prokaryotic and eukaryotic DNA replication differ in several key ways:
Prokaryotic DNA replication occurs continuously and in the cytoplasm, while eukaryotic replication is restricted to S phase of the cell cycle and takes place in the nucleus. Prokaryotic DNA is circular while eukaryotic DNA is linear. Prokaryotic DNA has a single origin of replication whereas eukaryotes have multiple origins. The processes utilize different DNA polymerases and prokaryotic replication is much faster at around 2000 nucleotides per second compared to 100 per second for eukaryotes. The final products are also different, with prokaryotes producing two circular chromosomes and eukaryotes producing two
This document discusses the absorption of UV light by nucleic acids and the denaturation and renaturation of DNA molecules. Some key points:
1. Nucleic acids absorb UV light due to their conjugated double bonds and ring structures, with absorption maximum at 260nm. More ordered structures absorb less light.
2. DNA denaturation involves separating the two strands by disrupting hydrogen bonds, usually through increased temperature. This is measured by increased UV absorption.
3. The melting temperature (Tm) is when half the DNA molecules are denatured. Molecules with more GC pairs have a higher Tm.
4. Denatured DNA can renature by re-
This document discusses ethanol fermentation and production. Saccharomyces cerevisiae yeast is commonly used to ferment sugars like glucose and fructose into ethanol and carbon dioxide through anaerobic fermentation. The production of ethanol involves preparing nutrient solutions and inoculum from raw materials like molasses or grains, performing fermentation in large tanks, and recovering ethanol through distillation. Ethanol fermentation is an important industrial process used worldwide to produce biofuel from various plant materials.
The document discusses bacterial spores, including their structure, formation through sporulation, and germination. It notes that spores form a protective structure that allows bacteria to survive unfavorable conditions. There are two types of spores - endospores form inside the cell while exospores form on the surface. Sporulation is triggered by starvation and takes 8-19 hours, concentrating the genetic material within protective coats. Germination occurs when conditions become favorable again and involves activation, initiation through effector recognition, and outgrowth of the new vegetative cell. Spore formation allows some bacteria to survive extreme environments.
Bacterial recombination can occur through generalized transduction, where bacterial viruses called bacteriophages inadvertently package bacterial DNA from one host and deliver it to another during infection. Bacterial chromosomes are usually circular DNA molecules located in the nucleoid region. Plasmids are smaller ring DNA molecules that also reside in bacteria and can help them survive stress. Gene mapping techniques like physical mapping using molecular markers and genetic mapping using restriction sites help determine the locus and distance between genes on bacterial chromosomes. These mapping techniques benefit medicine, agriculture, forensics, and environmental applications.
Methanogens are archaea that obtain energy through the anaerobic synthesis of methane from carbon dioxide, hydrogen, or organic compounds like acetate. They are strict anaerobes that thrive in environments rich in organic matter like the rumen, wetlands, and sediments. Chemolithotrophs are prokaryotes that obtain energy from the oxidation of reduced inorganic compounds like hydrogen, ammonia, and sulfide while fixing inorganic carbon. They generate ATP through oxidative phosphorylation using electrons from inorganic molecules in their electron transport chain rather than organic nutrients. Methanogens and chemolithotrophs play important ecological roles in biogeochemical cycling through the removal and fixation of compounds.
The bacterial cell has a small size between 0.5-2um and lacks membrane-bound organelles. It possesses a cell wall, plasma membrane, circular DNA located in the nucleoid region, ribosomes, and can contain inclusion bodies. The cell wall structure differs between gram-positive and gram-negative bacteria, with gram-positives having a thick peptidoglycan layer and gram-negatives having an additional outer membrane. Some bacteria possess appendages like flagella, pili, or fimbriae and structures like endospores, capsules, or S-layers may also be present outside the cell wall.
1) Microorganisms use fermentation when respiration is not possible due to a lack of an external electron acceptor. This allows regeneration of NAD+ from NADH to continue glycolysis.
2) Common fermentation pathways include alcoholic fermentation which produces ethanol, and lactic acid fermentation which produces lactate.
3) Fermentation allows oxidation of NADH and uses an intermediate such as pyruvate or its derivatives as the electron acceptor.
This document discusses various types of anaerobic respiration. It describes how anaerobic respiration works using electron acceptors other than oxygen, such as nitrates, sulfates, or carbon dioxide. It then examines different forms of anaerobic respiration in more detail, including denitrification, sulfate reduction, and sulfur disproportionation. Key enzymes and pathways involved in nitrate reduction, sulfate reduction, and other processes are outlined.
Genomic and cDNA libraries provide a collection of DNA fragments that can be screened to find genes of interest. To make a genomic library, genomic DNA is purified from cells, fragmented, and cloned into a vector such as lambda phage. A large number of clones are needed to represent the entire genome. cDNA libraries are made from mRNA which is isolated, reverse transcribed to cDNA, and cloned into a vector. Libraries are screened by hybridization, expression analysis or other methods to identify clones containing genes of interest. Repeated screening allows chromosome walking to obtain overlapping genomic fragments.
This document discusses site-specific recombination, including the structures and mechanisms involved. It describes two classes of recombinases - tyrosine recombinases and serine recombinases. Tyrosine recombinases involve cleavage of DNA through formation of a protein-DNA bond using a tyrosine residue. Serine recombinases utilize a phosphoserine bond between DNA and a conserved serine residue. The document provides examples of applications for site-specific recombination such as tracking cell lineage, altering gene expression, and targeted gene knockout.
Bacteriophage are viruses that infect and destroy bacteria. They were first observed in 1915 and have a simple structure with genetic material encapsulated in a protein coat. Bacteriophage have a life cycle where they adsorb to bacteria, penetrate the cell, use it to replicate their genetic material, assemble new viral particles, and are then released to infect more bacteria. There are an estimated 1031 bacteriophage on earth with a variety of types that infect different bacterial species. Bacteriophage therapy is being explored as an alternative to antibiotics for treating bacterial infections.
Oxygenic and anoxygenic photosynthesis can occur in microorganisms. Anoxygenic photosynthesis does not produce oxygen, using molecules like hydrogen sulfide instead of water. Oxygenic photosynthesis uses water and produces oxygen, occurring in cyanobacteria and eukaryotes. These two types of photosynthesis differ in their light-dependent and light-independent reactions and electron flow. Anoxygenic photosynthetic bacteria are diverse and use various electron donors besides water.
oxidation and reduction reaction in living system.pptxAzharAzhar63
Oxidation-redox reactions involve the transfer of electrons, with oxidation occurring when electrons are lost and reduction when electrons are gained. These reactions are important in living systems, powering processes like photosynthesis and respiration. Photosynthesis uses energy from sunlight to oxidize water and reduce carbon dioxide into organic molecules like glucose. Cellular respiration then oxidizes glucose and reduces oxygen, generating energy through redox reactions and recycling carbon dioxide and water. Redox reactions are thus vital to basic life processes and play a role in metabolic pathways through electron carrier molecules.
Endospores are dormant structures produced by certain bacteria, such as Bacillus and Clostridium, that allow them to survive unfavorable environmental conditions. Endospores have a layered structure that makes them highly resistant to heat, radiation, disinfectants and other stresses. When conditions improve, endospores can activate, germinate and grow out into normal vegetative bacterial cells through a process of reactivation. The ability of endospores to survive harsh conditions makes some bacteria dangerous pathogens and able to contaminate areas after being thought sterilized.
nitrate and sulfate reduction ; methanogenesis and acetogenesisjyoti arora
this powerpoint includes the following topics in brief:
1. Nitrate assimilatory reduction
2. Sulfate assimilatory reduction
3. Methanogenesis
4. Acetogenesis
This document summarizes the microbial production of two important antibiotics - penicillin and streptomycin. It describes that penicillin is produced by fermentation of Penicillium chrysogenum in a nutrient medium, and involves inoculum preparation, fermentation process under controlled conditions, and downstream recovery and purification of penicillin. Streptomycin is derived from Streptomyces griseus and its fermentation in Hockenhull medium occurs in three phases before recovery of the antibiotic. Both antibiotics have important medical uses for treating bacterial infections.
1. Enzyme activity can be regulated through several mechanisms including allosteric regulation, feedback inhibition, proenzymes, and protein modification.
2. Allosteric enzymes have effector molecules that bind and induce a conformational change that increases or decreases enzyme activity. Feedback inhibition occurs when a metabolic end product inhibits an earlier enzyme.
3. Proenzymes are inactive precursors that are activated by proteolytic cleavage. Protein modification like phosphorylation can also regulate enzymes by changing their structure.
Gluconeogenesis is the metabolic pathway by which glucose is synthesized from non-carbohydrate materials to maintain blood glucose levels during periods without food intake. It takes place primarily in the liver and involves bypasses of three irreversible steps in glycolysis. Precursors like lactate, glycerol, and certain amino acids are converted to pyruvate and then glucose. The pathway requires energy in the form of 6 ATP molecules to synthesize one glucose molecule from two pyruvate. Gluconeogenesis is important for supplying glucose to tissues like the brain and helps maintain normal blood sugar through processes like the Cori cycle.
Difference between prokaryotic & eukaryotic DNA replicationJacksonchary
Prokaryotic and eukaryotic DNA replication differ in several key ways:
Prokaryotic DNA replication occurs continuously and in the cytoplasm, while eukaryotic replication is restricted to S phase of the cell cycle and takes place in the nucleus. Prokaryotic DNA is circular while eukaryotic DNA is linear. Prokaryotic DNA has a single origin of replication whereas eukaryotes have multiple origins. The processes utilize different DNA polymerases and prokaryotic replication is much faster at around 2000 nucleotides per second compared to 100 per second for eukaryotes. The final products are also different, with prokaryotes producing two circular chromosomes and eukaryotes producing two
This document discusses the absorption of UV light by nucleic acids and the denaturation and renaturation of DNA molecules. Some key points:
1. Nucleic acids absorb UV light due to their conjugated double bonds and ring structures, with absorption maximum at 260nm. More ordered structures absorb less light.
2. DNA denaturation involves separating the two strands by disrupting hydrogen bonds, usually through increased temperature. This is measured by increased UV absorption.
3. The melting temperature (Tm) is when half the DNA molecules are denatured. Molecules with more GC pairs have a higher Tm.
4. Denatured DNA can renature by re-
This document discusses ethanol fermentation and production. Saccharomyces cerevisiae yeast is commonly used to ferment sugars like glucose and fructose into ethanol and carbon dioxide through anaerobic fermentation. The production of ethanol involves preparing nutrient solutions and inoculum from raw materials like molasses or grains, performing fermentation in large tanks, and recovering ethanol through distillation. Ethanol fermentation is an important industrial process used worldwide to produce biofuel from various plant materials.
The document discusses bacterial spores, including their structure, formation through sporulation, and germination. It notes that spores form a protective structure that allows bacteria to survive unfavorable conditions. There are two types of spores - endospores form inside the cell while exospores form on the surface. Sporulation is triggered by starvation and takes 8-19 hours, concentrating the genetic material within protective coats. Germination occurs when conditions become favorable again and involves activation, initiation through effector recognition, and outgrowth of the new vegetative cell. Spore formation allows some bacteria to survive extreme environments.
Bacterial recombination can occur through generalized transduction, where bacterial viruses called bacteriophages inadvertently package bacterial DNA from one host and deliver it to another during infection. Bacterial chromosomes are usually circular DNA molecules located in the nucleoid region. Plasmids are smaller ring DNA molecules that also reside in bacteria and can help them survive stress. Gene mapping techniques like physical mapping using molecular markers and genetic mapping using restriction sites help determine the locus and distance between genes on bacterial chromosomes. These mapping techniques benefit medicine, agriculture, forensics, and environmental applications.
Methanogens are archaea that obtain energy through the anaerobic synthesis of methane from carbon dioxide, hydrogen, or organic compounds like acetate. They are strict anaerobes that thrive in environments rich in organic matter like the rumen, wetlands, and sediments. Chemolithotrophs are prokaryotes that obtain energy from the oxidation of reduced inorganic compounds like hydrogen, ammonia, and sulfide while fixing inorganic carbon. They generate ATP through oxidative phosphorylation using electrons from inorganic molecules in their electron transport chain rather than organic nutrients. Methanogens and chemolithotrophs play important ecological roles in biogeochemical cycling through the removal and fixation of compounds.
The bacterial cell has a small size between 0.5-2um and lacks membrane-bound organelles. It possesses a cell wall, plasma membrane, circular DNA located in the nucleoid region, ribosomes, and can contain inclusion bodies. The cell wall structure differs between gram-positive and gram-negative bacteria, with gram-positives having a thick peptidoglycan layer and gram-negatives having an additional outer membrane. Some bacteria possess appendages like flagella, pili, or fimbriae and structures like endospores, capsules, or S-layers may also be present outside the cell wall.
1) Microorganisms use fermentation when respiration is not possible due to a lack of an external electron acceptor. This allows regeneration of NAD+ from NADH to continue glycolysis.
2) Common fermentation pathways include alcoholic fermentation which produces ethanol, and lactic acid fermentation which produces lactate.
3) Fermentation allows oxidation of NADH and uses an intermediate such as pyruvate or its derivatives as the electron acceptor.
This document discusses various types of anaerobic respiration. It describes how anaerobic respiration works using electron acceptors other than oxygen, such as nitrates, sulfates, or carbon dioxide. It then examines different forms of anaerobic respiration in more detail, including denitrification, sulfate reduction, and sulfur disproportionation. Key enzymes and pathways involved in nitrate reduction, sulfate reduction, and other processes are outlined.
Genomic and cDNA libraries provide a collection of DNA fragments that can be screened to find genes of interest. To make a genomic library, genomic DNA is purified from cells, fragmented, and cloned into a vector such as lambda phage. A large number of clones are needed to represent the entire genome. cDNA libraries are made from mRNA which is isolated, reverse transcribed to cDNA, and cloned into a vector. Libraries are screened by hybridization, expression analysis or other methods to identify clones containing genes of interest. Repeated screening allows chromosome walking to obtain overlapping genomic fragments.
This document discusses site-specific recombination, including the structures and mechanisms involved. It describes two classes of recombinases - tyrosine recombinases and serine recombinases. Tyrosine recombinases involve cleavage of DNA through formation of a protein-DNA bond using a tyrosine residue. Serine recombinases utilize a phosphoserine bond between DNA and a conserved serine residue. The document provides examples of applications for site-specific recombination such as tracking cell lineage, altering gene expression, and targeted gene knockout.
Bacteriophage are viruses that infect and destroy bacteria. They were first observed in 1915 and have a simple structure with genetic material encapsulated in a protein coat. Bacteriophage have a life cycle where they adsorb to bacteria, penetrate the cell, use it to replicate their genetic material, assemble new viral particles, and are then released to infect more bacteria. There are an estimated 1031 bacteriophage on earth with a variety of types that infect different bacterial species. Bacteriophage therapy is being explored as an alternative to antibiotics for treating bacterial infections.
Oxygenic and anoxygenic photosynthesis can occur in microorganisms. Anoxygenic photosynthesis does not produce oxygen, using molecules like hydrogen sulfide instead of water. Oxygenic photosynthesis uses water and produces oxygen, occurring in cyanobacteria and eukaryotes. These two types of photosynthesis differ in their light-dependent and light-independent reactions and electron flow. Anoxygenic photosynthetic bacteria are diverse and use various electron donors besides water.
oxidation and reduction reaction in living system.pptxAzharAzhar63
Oxidation-redox reactions involve the transfer of electrons, with oxidation occurring when electrons are lost and reduction when electrons are gained. These reactions are important in living systems, powering processes like photosynthesis and respiration. Photosynthesis uses energy from sunlight to oxidize water and reduce carbon dioxide into organic molecules like glucose. Cellular respiration then oxidizes glucose and reduces oxygen, generating energy through redox reactions and recycling carbon dioxide and water. Redox reactions are thus vital to basic life processes and play a role in metabolic pathways through electron carrier molecules.
1. Photosynthesis is the process by which plants use sunlight, carbon dioxide and water to produce oxygen and energy in the form of glucose.
2. Early experiments by Priestley and Ingenhousz showed that plants release oxygen and restore air contaminated by animals, with Ingenhousz showing sunlight is required.
3. Later experiments determined the sites of photosynthesis within plants (chloroplasts and leaves), identified pigments like chlorophyll, and established the basic chemical equation of photosynthesis involving carbon dioxide, water, oxygen and glucose.
This document provides an overview of photosynthesis. It explains that photosynthesis requires carbon dioxide, water, and sunlight as inputs. The light-dependent reactions use sunlight to produce ATP and NADPH, while the light-independent reactions use ATP and NADPH to produce glucose from carbon dioxide. Photosynthesis is important as it provides food for other organisms, removes carbon dioxide from the atmosphere, and supports industrial activities that rely on plant materials.
This document summarizes plant nutrition and photosynthesis. It discusses that plants are autotrophs that produce their own food through photosynthesis, using carbon dioxide, water, and sunlight to produce glucose and oxygen. The process involves light and dark reactions that take place in the chloroplasts of leaf cells. Photosynthesis is affected by factors like carbon dioxide, temperature, and light levels. The document also describes leaf structure and mineral nutrition, noting that plants require macronutrients and micronutrients to carry out their functions.
9 - Metabolism and Transfering Energy - Part TwoAhmad V.Kashani
سلولهای زنده برای انجام بسیاری از وظایف خود به انتقال انرژی از منابع خارجی نیاز دارند. همه ارگانیسمها باید از طریق فتوسنتز و تنفس سلولی این انرژی را از مولکول های آلی موجود درغذا بدست آورند. تنفس با استفاده از اکسیژن و تولید ATP، باعث شکستن این سوخت میشود. مواد زائد این نوع تنفس، دی اکسید کربن و آب، مواد اولیه فتوسنتز هستند. در این اسلاید، من سعی می کنم چگونگی برداشت سلولها از انرژی ذخیره شده در مولکولهای آلی و استفاده از آن برای تولید ATP از طریق تنفس سلولی را توضیح دهم.
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Living cells require transfusions of energy from outside sources to perform their many tasks. All organism need to obtain this energy from organic molecules of food through photosynthesis and cellular respiration. Respiration breaks this fuel down, using oxygen and generating ATP. The waste products of this type of respiration, carbon dioxide and water, are the raw materials for photosynthesis. In this slide, I try to explain how cells harvest this energy stored in organic molecules and used it to generate ATP through cellular respiration.
Photosynthesis occurs in chloroplasts and involves two main stages. In the light-dependent reactions, chlorophyll absorbs light energy which splits water molecules into oxygen, protons, and electrons. The Calvin cycle then uses these products to convert carbon dioxide into glucose through chemical reactions, producing oxygen as a byproduct. Photosynthesis is essential as it is how energy from the sun is captured and stored as chemical energy in sugars, the primary food source for most living things.
Photosynthesis is a two-stage process by which plants use sunlight, carbon dioxide, and water to produce oxygen and sugars like glucose. In the light reactions, chlorophyll in plant leaves absorbs sunlight to provide energy to convert carbon dioxide and water into oxygen and energy carriers like ATP. In the dark reactions, the energy carriers are used to produce sugars from carbon dioxide. Photosynthesis is essential for plants to survive and produces oxygen for other living things to survive.
Photosynthesis is the process by which plants, algae, and some bacteria use sunlight, water, and carbon dioxide to produce oxygen and energy in the form of glucose. It occurs in two stages - the light-dependent reaction where sunlight is absorbed to make ATP and NADPH, and the light-independent reaction where carbon dioxide is fixed using ATP and NADPH to make glucose. Photosynthesis takes place in chloroplasts within plant cells and is essential for life on Earth as it produces the oxygen needed for respiration and food in the form of glucose.
Photosynthesis is the process by which plants produce carbohydrates from carbon dioxide and water using energy from sunlight. It occurs in chloroplasts, which contain chlorophyll and are located in plant leaves. There are two phases - the light-dependent reaction uses solar energy to produce ATP and NADPH, while the light-independent Calvin cycle uses these products to fix carbon from carbon dioxide into glucose. Photosynthesis oxygenates the atmosphere and provides a crucial food source for the biological world.
The document summarizes photosynthesis and the Calvin cycle. It describes how:
1) Photosynthesis uses light energy from the sun, carbon dioxide, and water to produce oxygen and energy-rich organic molecules like glucose.
2) The Calvin cycle fixes carbon dioxide into organic molecules like glucose using ATP and NADPH produced in the light reactions. It involves carboxylation, reduction, and regeneration of the carbon acceptor RuBP.
3) Rubisco catalyzes the carboxylation of RuBP to form two molecules of 3-phosphoglycerate. Triose phosphates are formed through reduction using ATP and NADPH. Ribulose-1,5-bisphosphate is regenerated for continuous carbon
Respiration is the process by which glucose is oxidized to produce energy. It occurs via three main stages - glycolysis, the citric acid cycle, and oxidative phosphorylation. Glycolysis breaks down glucose into pyruvate and produces a small amount of ATP. The citric acid cycle further oxidizes pyruvate and generates more ATP. Oxidative phosphorylation uses the electrons from NADH and FADH2 to power ATP synthase to produce the majority of ATP. Respiration is essential for producing the energy currency ATP that cells need to carry out life functions.
mechanism of photosysthesis PPT, SSC AP srinivas nallapuSrinivas Nallapu
Photosynthesis uses light energy from the sun to convert carbon dioxide and water into oxygen and energy-rich organic compounds, especially glucose. This process involves two stages: the light-dependent reactions where ATP and NADPH are produced, and the light-independent reactions (Calvin cycle) where carbon is incorporated into organic compounds. The light reactions occur in the thylakoid membranes of the chloroplast and use chlorophyll to drive the production of ATP and NADPH. These products are then used in the Calvin cycle to reduce carbon dioxide into glucose.
The document provides an overview of photosynthesis through multiple sections. It defines photosynthesis as the process where light energy is absorbed by chlorophyll and converted to chemical energy, which is used to synthesize glucose from carbon dioxide and water, releasing oxygen. It describes the light dependent and light independent reactions, including where they take place, their requirements and products. It also outlines the necessary conditions, affecting factors, importance and implications if photosynthesis did not occur.
1. The document discusses various types of photosynthesis including non-cyclic and cyclic photophosphorylation, C3 and C4 pathways, and CAM pathway.
2. It also describes bacterial photosynthesis, distinguishing between anoxygenic photosynthesis which does not produce oxygen, and oxygenic photosynthesis which does produce oxygen as a byproduct.
3. The key stages of photosynthesis are explained in detail, including the light-dependent and light-independent reactions, and how ATP and NADPH are generated to ultimately produce glucose.
Photosynthesis is the process by which plants, algae, and some bacteria use sunlight, water, and carbon dioxide to produce oxygen and energy in the form of glucose. It occurs in two phases - the light reactions where sunlight is absorbed and used to produce ATP and NADPH, and the Calvin cycle where ATP and NADPH fuel the reduction of carbon dioxide into sugars. The majority of life on Earth depends on photosynthesis to convert the sun's energy into chemical energy stored in sugars.
This document provides an overview of photosynthesis. It begins by defining photosynthesis as the process by which plants and algae use sunlight, water and carbon dioxide to produce oxygen and energy in the form of ATP and NADPH. It then discusses the historical background and discovery of photosynthesis. The remainder of the document describes the key components of photosynthesis, including the two photochemical processes (photosystem I and photosystem II), the light-dependent reactions, and the mechanisms of cyclic and non-cyclic photophosphorylation that generate ATP using energy from excited electrons. In closing, it thanks the reader and lists references used.
This document provides an overview of photosynthesis. It begins by defining photosynthesis as the process by which plants and algae use sunlight, water and carbon dioxide to produce oxygen and energy in the form of glucose. It then discusses the historical background and discovery of photosynthesis. The remainder of the document describes the key components of photosynthesis, including the two photochemical processes (photosystems I and II), the light-dependent reactions, and the mechanisms of cyclic and non-cyclic photophosphorylation that generate ATP using energy from excited electrons. In closing, it summarizes the similarities and differences between cyclic and non-cyclic photophosphorylation.
This document summarizes key concepts in bioenergetics and cellular respiration. It discusses how living organisms obtain and use energy through redox reactions and electron carriers like ATP. Photosynthesis and cellular respiration are introduced as the two main pathways of energy transformation. Photosynthesis uses energy from sunlight to synthesize glucose from carbon dioxide and water, while cellular respiration breaks down glucose to release energy through glycolysis, the Krebs cycle, and the electron transport chain. The document aims to explain the basic processes of how energy is transformed and utilized in living cells and organisms.
1. The document discusses cellular respiration in plants. It defines respiration as the process by which organisms obtain energy by combining oxygen and glucose.
2. Photosynthesis provides plants with oxygen and organic compounds through chloroplasts, while respiration breaks down these compounds in cytoplasm and mitochondria to release energy through ATP.
3. The process of respiration involves glycolysis, the Krebs cycle, and the electron transport chain. Glycolysis occurs in the cytoplasm and partially oxidizes glucose to pyruvate, the Krebs cycle fully oxidizes pyruvate in the mitochondria producing carbon dioxide, and the electron transport chain uses oxygen to produce ATP through oxidative phosphorylation.
Similar to PHOTOSYNTHETIC BACTERIA (OXYGENIC AND ANOXYGENIC) (20)
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2. INTRODUCTION
• All the living organism require energy to carry out their
different activities of life for this energy is needed which
comes by the oxidation of carbohydrates, proteins, fat
similar to green plant ,these have certain chlorophyll
containing compound which synthesis food
from simple carbon dioxide, water.
• Photosynthesis in bacteria defined as the synthesis of
the carbohydrate by the chlorophyll in the presence of
similar compound such as carbon dioxide and reductanct
taken from the air and oxygen does not evolve as a
product
2H2A+CO2----------------------(CH2O)x+2A+2H2O
3. • All the photosynthetic bacteria are classified into the 35
groups.The group 10 contain anoxygenic phototrophic
bacteria whlie 11 belongs to oxygenic phototropic
bacteria .
• The anoxygenic group has purple and green sulphur
bacteria and oxygenic group has cyanobacteria .
• Another type of oxygenic bacteria are
prochlorophyta. It acts as a bridge between cyanophyta
and chlorophyta.
4. CHLOROPHYLL
• Chlorophyll is a complex
molecule .several modification of
chlorophyll occur among plant
and other photosynthetic
organism .All photosynthetic
organism have chlorophyll a and
accessory pigment.
• Accessory pigment contain
chlorophyllb, c ,d and e
,Xanthophyll and carotenoids.
• It absorb energy from different
wavelength such as Voilet-blue,
reddish ,orange –red etc.
5. STAGE OF PHOTOSYNTHESIS
• When chlorophyll absorb light
energy ,an electron gain energy
and exicted .The excited
electron is transferred to
another molecule (called
primary electron acceptor)
• The chlorophyll molecule is
oxidized (loss of electron) and
has a positive charge.
• Photoactivation of chlorophyll
results a splitting of the water
molecule and transfer of
energry to ATP and NADP+.
6CO2+12 H2O+light----C6H12O6+6O2+6H2O
6. TYPES OF PHOTOSYNTHESIS
There are two types of photosynthesis
1. Anoxygenic Photosynthesis -phototrophic bacteria H2O is not
oxidized and O2 is not produced, and thus the process is called
anoxygenic photosynthesis.
CO2+2H2A+Light--[CH20]+2A
Example-Purple bacteria
H2A=H2O,H2S,H2 etc.
2. Oxygenic Photosynthesis-The oxidation of H2O produces
molecular oxygen (O2) as a by-product. Because O2 is produced,
photosynthesis in these organisms is called oxygenic
photosynthesis.
6CO2+12 H2O+light-----C6H12O6+6O2+6H2O
Example-Eukaryotes and cyanobacteria
2H2O 4H++O2+4e-
7. ANOXYGENIC PHOTOSYNTHESIS
• It is type of photosynthesis process which frequently occurs in
the microorganism which are mostly found in aquatic habitat .
This reaction does not involve production of oxygen.
• Sulfur is used as a reducing agent during the process in green
sulfur bacteria and purple bacteria.
8. ANOXYGENIC PHOTOSYNTHETIC BACTERIA
• Some photosynthetic bacteria can use light energy to extract
electrons from molecules other than water.
• These organisms are of ancient origin, presumed to have evolved
before oxygenic photosynthetic organisms.
• Anoxygenic photosynthetic organisms occur in the domain
Bacteria and have representatives in four phyla – Purple-Sulphur
Bacteria, Purple non- Sulphur Bacteria, Green-Sulfur Bacteria,
Green non- Sulfur Bacteria.
• Anoxygenic photosynthesis depends on electron donors such as
reduced sulphur compounds, molecular hydrogen or organic
compounds.
• They are found in fresh water, brackish water, marine and
hypersaline water.
• Anoxygenic photosynthetic bacteria have been divided into three
groups on the basis of pigmentation: purple bacteria, green bacteria
and heliobacteria.
9. OXYGENIC PHOTOSYNTHETIC BACTERIA
• The Oxygenic Photosynthetic Bacteria are
unicellular or multicellular and possess
bacteriochlorophyll a and carry out oxygenic
photosynthesis.
• They are mostly represented by gram-
negative cynobacteria.
• Carboxysomes and gas vesicles are present
and also show gliding movement.
• Photosynthesis is oxygenic and autotrophic.
• Photosynthates get accumulated in the
form ofglycogen.
10. OXYGENIC PHOTOSYNTHESIS
• During this process ,light energy transfer electron
from water to carbon dioxide to produce
carbohydrate .In this reaction the carbon dioxide is
reduced or receive the electron and water become
oxidised or losses electron .Ultimately oxygen is
produced along with carbohydrate.
6CO2+12 H2O+light----C6H12O6+6O2+6H2O
This is a two stage process
1. Light dependent reaction
2. Light independent reaction
11. • Light dependent reaction-This is a series of
reaction which occurs in the grana of chloroplast
and require direct energy from the sulight .Light
energy is trapped for the ATP synthesis at some time
water is photolysed in to Oxygen and Hydrogen ion
and free electron carrier.
2H2O 4H++O2+4e-
• Light Independent reaction – A light
independent reaction is a series of reaction in the
stroma of chloroplast .It help to make the
carbohydrate from carbon dioxide molecule by
reduction process .
12.
13. • In contrast to electron flow in anoxygenic
phototrophs, electron flow in oxygenic phototrophs
proceeds through two distinct but interconnected
series of light reactions.
• The two light systems are called photosystem I and
photosystem II, each photosystem having a
spectrally distinct form of reaction center
chlorophyll a.
• Photosystem I (PSI) chlorophyll, called P700,
absorbs light at long wavelengths (far red light),
whereas Photosystem II chlorophyll, called
P680, absorbs light at shorter wavelengths (near
red light).
• Oxygenic phototrophs use light to generate both
ATP and NADPH, the electrons for the latter
arising from the splitting of water into oxygen and
electrons.