Next-generation sequencing (NGS) technologies offer cost-effectiveness, speed, and accuracy, significantly benefiting oncology and immunology. The document outlines the sequencing process involving DNA library preparation, clonal amplification, and cyclic array sequencing. Creative Biolabs provides various NGS services for cancer research, including whole genome and transcriptome sequencing.

1. Next-generation sequencing
(NGS) technologies have
progressive advantages in
terms of cost-effectiveness,
unprecedented sequencing
speed, high resolution and
accuracy in genomic analy-
ses, thus are playing an
increasing important role in
fields of oncology and immu-
nology.
The sequencing principles of
common sequencing instru-
ments on the market: (1) first,
construct a DNA template
library. Obtain DNA library
fragments (tens to hundreds
of bases in length) by
randomly breaking genomic
DNA, or construct paired-end
fragments that control the
distance distribution. Adapter
sequences were ligated to
both ends of the DNA
fragment and then denatured
to obtain a single-stranded
template library and immobi-
lized on a solid surface. (2)
Second, amplify the clones,
which performed by one of
several methods, such as
bridge PCR and emulsion
PCR. (3) Then, forming DNA
clusters or amplifying micro-
spheres of DNA cluster arrays
on a chip, performing a series
of cyclic reaction operations
using a polymerase or ligase,
and monitoring the optical
events generated in each
cycle biochemical reaction by
a microscopic detection syst-
em. Time series analysis of
the resulting array images is
performed to obtain sequenc-
es of DNA fragments. These
fragments are then assem-
bled into longer contigs
according to certain computer
algorithms.
Creative Biolabs uses the
advanced SuPrecision™ pl-
atform to support researchers
all over the world with their
sequencing needs for cancer.
NEXT GENERATION
SEQUENCING SERVICE
Creative Biolabs
Next Generation
Sequencing
Service
© 2007 - 2019 Creative-Biolabs All Rights Reserved Email: info@creative-biolabs.com Tel: 1-631-381-2994
WHAT WE DO:
Whole Genome Sequencing (WGS)
Service for Cancer
Whole Exome Sequencing (WES)
Service for Cancer
Targeted Sequencing Service for
Cancer
Whole Transcriptome Sequencing (WTS)
Service for Cancer
Immune Repertoire Sequencing (Rep-seq)
Service for Cancer
Bioinformatics Analysis Service

2. DNA Library Preparation
1 Clonal Amplification
2
Cyclic Array Sequencing
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