Md. Abdul Momin presented on DNA microarray technology. Microarrays allow researchers to analyze gene expression levels of thousands of genes simultaneously using DNA probes attached to a solid surface. The presentation covered the history and principles of microarray technology, types of microarrays including glass cDNA and in situ oligonucleotide arrays, and applications such as disease diagnosis, drug discovery, and toxicology research. Microarrays are a powerful tool for functional genomics and comparative analysis across many fields of study.
Next generation Sequencing or massive parallel sequencing is a high throughput approach to sequence genetic material using the concept of massively parallel processing. It is also called second generation sequencing.This enables researchers a wide variety of applications & study biological systems.
NGS is a new way to examine and explore genes. Sanger's method is still being used in science but has some difficulties. This presentation covers only two types of NGSs but they are good examples of better ways to study genes, genome. NGS are really important nowadays in diagnostics tumors and cancers.
Next generation Sequencing or massive parallel sequencing is a high throughput approach to sequence genetic material using the concept of massively parallel processing. It is also called second generation sequencing.This enables researchers a wide variety of applications & study biological systems.
NGS is a new way to examine and explore genes. Sanger's method is still being used in science but has some difficulties. This presentation covers only two types of NGSs but they are good examples of better ways to study genes, genome. NGS are really important nowadays in diagnostics tumors and cancers.
Deciphering DNA sequences is essential for virtually all branches of biological research. With the
advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to
elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
Explore the Illumina workflow, including sequencing by synthesis (SBS) technology, in 3-dimensional detail. Go from sample preparation, to cluster generation, to sequencing on a system flow cell with the proprietary SBS process.
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
A class of DNA sequencing techniques currently in active development is third-generation sequencing, commonly referred to as long-read sequencing. In comparison to second generation sequencing, also referred to as next generation sequencing, third generation sequencing technologies have the capacity to create noticeably longer reads.
Next Generation Sequencing (NGS) Is A Modern And Cost Effective Sequencing Technology Which Enables Scientists To Sequence Nucleic Acids At Much Faster Rate. In This Presentation, You Will Learn About What is NGS, Idea Behind NGS, Methodology And Protocol, Widely Adapted NGS Protocols, Applications And References For Further Study.
Deciphering DNA sequences is essential for virtually all branches of biological research. With the
advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to
elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
Explore the Illumina workflow, including sequencing by synthesis (SBS) technology, in 3-dimensional detail. Go from sample preparation, to cluster generation, to sequencing on a system flow cell with the proprietary SBS process.
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
A class of DNA sequencing techniques currently in active development is third-generation sequencing, commonly referred to as long-read sequencing. In comparison to second generation sequencing, also referred to as next generation sequencing, third generation sequencing technologies have the capacity to create noticeably longer reads.
Next Generation Sequencing (NGS) Is A Modern And Cost Effective Sequencing Technology Which Enables Scientists To Sequence Nucleic Acids At Much Faster Rate. In This Presentation, You Will Learn About What is NGS, Idea Behind NGS, Methodology And Protocol, Widely Adapted NGS Protocols, Applications And References For Further Study.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes.
This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
The above presentation consist of the definition of microarray, brief history, general principle of the same, the type of scanner that are used to read or to scan the microarray , type of DNA microarray and finally its various apliccation including the role of DNA microaarray in drug discovery.
Molecular Biology research evolves through the development of the technologies used for carrying them out. It is not possible to research on a large number of genes using traditional methods
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
3. 1. INTRODUCTION
2. HISTORY
3. PRINCIPLE
4. DNA MICROARRAY TECHNOLOGY
5. PRINCIPLES OF DNA MICROARRAY TECHNOLOGY
6. TYPES OF DNA MICROARRAY
GLASS cDNAMICROARRAYS
IN SITU OLIGONUCLEOTIDEARRAY FORMAT
7.APPLICATIONS OF MICROARRAY TECHNOLOGY
CONTENTS:
4. INTRODUCTION:
The large-scale genome sequencing effort and the ability
to immobilize thousands of DNA fragments on coated
glass slide or membrane, have led to the development of
microarray technology.
A microarray is a pattern of ssDNA probes which are
immobilized on a surface called a chip or a slide.
Microarrays use hybridization to detect a specific DNA or
RNAin a sample.
DNA microarray uses a million different probes, fixed on
a solid surface.
5. An array is an orderly
arrangement of samples
where matching of known and
unknown DNA samples is
done based on base pairing
rules.
An array experiment makes
use of common assay systems
such as microplates or
standard blotting membranes.
What is An Array
Fig-Robotic arm with spotting slides
6. HISTORY
Microarray technology evolved from Southern blotting.
The concept of microarrays was first proposed in the late
1980s byAugenlicht and his colleagues.
They spotted 4000 cDNAsequences on nitrocellulose
membrane and used radioactive labeling to analyze
differences in gene expression patterns among different
types of colon tumors in various stages of malignancy
7. PRINCIPLE
The core principle behind
microarrays is hybridization
between two DNAstrands.
Fluorescent labeled target
sequences that bind to a
probe sequence generate a
signal that depends on the
strength of the hybridization
determined by the number of
paired bases.
Fig- Array hybridization
8. DNA MICROARRAY TECHNOLOGY
DNA microarray technology may be defined as a high-throughput
and versatile technology used for parallel gene expression
analysis for thousands of genes of known and unknown functions.
Used for detection of polymorphisms and mutations in genomic
DNA .
A DNA microarray is a collection of microscopic DNA spots on
solid surface. Each spot contains picomoles of a specific DNA
sequence, known as probes or reporters.
Each identified sequenced gene on the glass, silicon chips or
nylon membrane corresponds to a fragment of genomic DNA,
cDNAs, PCR products or chemically synthesized oligonucleotides
of up to 70mers and represents a single gene.
Probe-target hybridization is usually detected and quantified by
detection of fluorophore, silver, or chemiluminescence labeled
targets to determine relative abundance of nucleic acid
sequences in the target.
9.
10. PRINCIPLES OF DNA MICROARRAY
TECHNOLOGY:
The principle of DNA microarray technology is based on
the fact that complementary sequences of DNA can be
used to hybridise, immobilised DNA molecules.
There are four major steps in performing a typical
microarray experiment.
11. Isolate a total RNA containing mRNA
that ideally represents a quantitative
copy of genes expressed at the time
of sample collection.
Preparation of cDNA from mRNA
using a reversetranscriptase
enzyme.
Short primer is required to initiate
cDNAsynthesis.
Each cDNA(Sample and Control) is
labelled with fluorescent cyanine
dyes (i.e. Cy3 and Cy5).
SAMPLE RREPARATION AND LABELING
Fig- Sample labeling
12. Here, the labelled cDNA
(Sample and Control) are
mixed together.
Purification
After purification, the mixed
labelled cDNA is competitively
hybridised against denatured
PCR product or cDNA
molecules spotted on a glass
slide.
ARRAY HYBRIDISATION
Fig-Array Hybridisatio
13. TYPES OF DNA MICROARRAY
1.Glass cDNA microarrays which involves the micro spotting
of pre-fabricated cDNA fragments on a glass slide.
2.High-density oligonucleotide microarrays often referred to
as a "chip" which involves in situ oligonucleotide synthesis.
Glass cDNA microarrays was the first
type of DNA microarray technology
developed.
It was pioneered by Patrick Brown and
his colleagues at Stanford University.
Produced by using a robotic device
which deposits (spots) a nanoliter of
DNA onto a coated microscopic glass
slide (50-150 µm in diameter).
GLASS cDNA MICROARRAYS
Fig-Contact printer with robotic pins
14. Advantages of Glass cDNA microarrays include their
relative affordability with a lower cost.
Its accessibility requiring no specific equipment for use such
that hybridisation does not need specialised equipment.
Data capture can be carried out using equipment that is
very often already available in the laboratory.
Advantages of Glass cDNA microarrays:
Glass cDNA microarray have a few disadvantages such as
intensive labour requirement for synthesizing, purifying, and
storing DNAsolutions before microarray fabrication.
They may hybridise to spots designed to detect transcript
from a different gene.
Disadvantages of Glass cDNA microarrays
15. Oligonucleotides are synthesized on the chip.
Presently, the commercial versions of Affymetrix Gene
Chips hold up to 500,000 probes/sites in a 1.28-cm2 chip
area.
Due to such very high information content (genes) they are
finding widespread use in the hybridisation-based detection
and analysis of mutations and polymorphisms, such as
single nucleotide polymorphisms.
IN SITU OLIGONUCLEOTIDE
ARRAY FORMAT:
16. Light is directed through a
photolithographic mask to specific
areas of array surface.
Activation of areas for chemical
coupling. Attachment of A
nucleotide containing photolabile
protecting group X (MeNPOC).
Next light is Directed to a different
region of the array surface through
a new mask.
Addition of 2nd building block T
containing a photolabile protecting
group X. This process is repeated
until the desired product is
obtained.
In Situ Light-Directed Oligonucletide
Probe Array Synthesis:
Fig-Photolithography process
17. Advantages offered by the in situ oligonucleotide array
format include speed, specificity and reproducibility.
In situ oligonucleotide array formats tend to have expensive
specialised equipments e.g. to carry out the hybridisation,
staining of label, washing, and quantitation process.
Short-sequences used on the array have decreased
sensitivity/binding compared with glass cDNA microarrays.
Advantages In of situ oligonucleotide
array formats
Diadvantages In of situ oligonucleotide array
formats
18.
19. The principle aim of using microarray technology as a gene
expression profiling tool is to answer some of the fundamental
questions in biology such as "when, where, and to what
magnitude genes of interest are expressed.
Microarray analysis measure changes in the multigene patterns
of expression to better understand about regulatory
mechanisms and broader bioactivity functions of genes .
Microarray as a Gene Expression
Profiling Tools:
20. Microarray technology have widespread use in comparative
gene mutation analysis to analyse genomic alterations such
as sequence and single nucleotide polymorphisms.
In microbiology microarray gene mutation analysis is
directed to characterisation of genetic differences among
microbial isolates, particularly closely related species.
Microarray as a Comparative
Genomics Tools:
21. Different types of cancer have been classified on the basis of
the organs in which the tumors develop.
Now, with the evolution of microarray technology, it will be
possible for the researchers to further classify the types of
cancer on the basis of the patterns of gene activity in the
tumor cells.
Disease Diagnosis:
Microarray technology has extensive application in
Pharmacogenomics.
Comparative analysis of the genes from a diseased and a
normal cell will help the identification of the biochemical
constitution of the proteins synthesized by the diseased
genes.
Drug Diacovery:
22. Microarray technology provides a robust platform for the
research of the impact of toxins on the cells and their
passing on to the progeny.
Toxicogenomics establishes correlation between responses
to toxicants and the changes in the genetic profiles of the
cells exposed to such toxicants.
The microarray permits researchers to examine thousands
of different genes in the same experiment and thus to obtain
a good understanding of the relative levels of expression
between different genes in an organism.
Toxicological Research:
23. Microarray is a recently developed functional genomics
technology that has powerful applications in a wide array of
biological medical sciences, agriculture, biotechnology and
environmental studies. Since many universities research
institutions and industries have established microarray
based core facilities and services, microarrays have
become a readily accessible, widely used technology for
investigating biological systems.
Conclusion: