The document provides an overview of next-generation sequencing (NGS) technologies and services offered by Creative Biolabs. It details various NGS methods, including library preparation, clonal amplification, sequencing data analysis, and specific services for cancer research such as whole genome sequencing and bioinformatics analysis. Additionally, contact information is provided for further inquiries.

1. Next Generation
Sequencing
(NGS)
w w w . c r e a t i v e - b i o l a b s . c o m 45-1 Ramsey Road, Shirley, NY 11967, USA
info@creative-biolabs.com
1-631-603-9819

2.  Illumina Genome Analyzer
NGS Systems
 454 Sequencer
 SOLiD System
 Ion Proton

3. Contents
Library Preparation 4
5
7
11
Clonal Amplification
Sequencing
Data Analysis

4. DNA/cDNA
A
A
A
A
A
A
A
A A
A
Library Preparation
Next Generation Sequencing
Fragmentation
End Repair
A-Tailing
PCR
Adaptor
Additon
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5. Clonal Amplification
Next Generation Sequencing
2 3
456
87
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-Bridge PCR
1

6. Beads
Emulsion Oli
DNA
Cluster
Clonal Amplification
Next Generation Sequencing
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-Emulsion PCR

7. Primer
Template
Terminator Caps
Fluorescent Dye
Fluorescent
Emission
Cleavage
Sequencing
Next Generation Sequencing
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-Synthesis

8. Template
Primer
DNA Polymerase
dNTP
Time
Intensity
Sequencing
Next Generation Sequencing
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-Pyrosequencing
Sulfurylase
APS
PPi
ATP
Luciferin
Luciferase
ATP

9. Sequencing
Next Generation Sequencing
Emulsion
Bead
Ligase
Primer
P1 Adapter Template
Cleavage
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Cleavage Site
-Ligation

10. Semiconductor
Transistor
dNTP
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- Ion Torrent semiconductor
Next Generation Sequencing
Sequencing

11. GTCGTATCTAAGTAC
C
TATGTAAGTACCACGGATGATA
CTAAGTACCACGGATGATAGCTT
AC
TACCACGGATGATAGCTTACGCT
AA
GTCGTATCTAAGTACCACGGATGATAGCTTACGCTA
A
Data Analysis
Next Generation Sequencing
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12. Whole Genome
Sequencing (WGS)
Service for Cancer
Whole Exome
Sequencing (WES)
Service for Cancer
Targeted Sequencing
Service for Cancer
Whole Transcriptome
Sequencing (WTS) Service
for Cancer
Immune Repertoire
Sequencing (Rep-seq)
Service for Cancer
Bioinformatics Analysis
Service
One-stop Service for
Cancer 3D Model
Epigenomics Service
Three-dimensional (3D)
Genomics Service
Metagenomics Sequencing
Service for Cancer
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Creative Biolabs Services
Next Generation Sequencing

13. Contact Us
45-1 Ramsey Road, Shirley, NY 11967, USA
info@creative-biolabs.com
1-631-603-9819
www.creative-biolabs.com