details of NGS

1. Next-Generation Sequencing
(NGS)
An Overview of Technology,
Methods, and Applications

2. Introduction to NGS
• NGS determines the order of nucleic acid
residues in biological samples. It has
revolutionized genetic research, making
sequencing faster and more affordable.

3. Advantages of NGS
• - High throughput: Thousands of genes
sequenced simultaneously
• - Low sample input requirements
• - High accuracy and sensitivity
• - Cost-effective compared to traditional
sequencing

4. NGS Methods
• - Whole-Genome Sequencing (WGS)
• - Targeted Sequencing (Exome & Gene Panels)
• - Amplicon-Based Approaches
• - Hybridization Capture

5. NGS Workflow
• 1. Sample Preparation
• 2. Library Preparation
• 3. Sequencing
• 4. Data Analysis & Interpretation

6. Applications of NGS
• - Cancer Research
• - Infectious Disease Research
• - Reproductive Health
• - Microbiology & Virology
• - Personalized Medicine

7. Conclusion
• NGS is a powerful technology enabling rapid
genomic analysis. It is essential for clinical
research, diagnostics, and precision medicine.