A presentation on Microarray and its possible real world application

1. DNA Microarray Analysis
Jay Edneil C. Olivar

2. DNA Microarray

3. DNA Microarray
The Result

4. DNA Microarray: The Experiment
Healthy Cell vs. Cancer Cell

5. DNA Microarray: mRNA isolation
Aliquot of
RNA
Column with poly-T
beads

6. DNA Microarray: the FUN part
Polymerization of
labeled cDNA
Green: Healthy
Red: Cancer

7. DNA Microarray: Results
Normal
Cancer
Combined

8. DNA Microarray: Results
Upregulated in
cancer cells
Downregulated in
cancer cells
Expressed in both
cells
Not Expressed in
both cells

9. Limits to Microarray analysis
Measure of gene
expression is solely
based on the
production of an
mRNA

10. For more information
• Visit:
http://learn.genetics.utah.edu/content/labs/micro
array/

11. Advantages and Disadvantages
Advantages Disadvantages
Simultaneous analysis of
multiple genes
Confusing to first time
users
High throughput Result not always
reproducible
Allows comparison of
gene expression (healthy
vs. non-healthy)
Gene expression is
dictated by qualitative
data
Can categorize diseases
into subgroups
Still too expensive

13. Objective
•To determine ANEUPLOIDY
RATES and INCIDENCE OF
MOSAICSIM in good-quality
human preimplantation
embryos using an array-based
technique

14. Aneuploidy and Mosaicism
•Aneuploidy
• Condition wherein chromosome
number is abnormal
•Mosaicism
• A condition wherein an individual has
two or more cell populations with a
different chromosomal makeup

15. Methodology
10 embryos (70 blastomeres) from 6 successful in vitro
fertilization was subject to testing
Whole genome extraction; Amplification
Array Hybridization
Computational Analysis

16. Microarray in the Experiment
22 x
22
22 x
22
22 x
22
22 x
22
Printed twice
Represents about
7744 genes
1. Plated genes were
designed to contain all
possible aberrations
2. Detection of aberrations
was done using the
CIRCULAR BINARY
SEGMENTATION (CBS)
method
3. DNA probing done through
Cy3 and Cy5 labeled probes

17. Results
80
70
60
50
40
30
20
10
0
Embryos
Normal Mosaic
60
50
40
30
20
10
0
Blastomeres
Normal
With Chromosomal Abnormalities

18. Conclusion and Implication
•High rate of aneuploidy and
mosaicism in excellent quality
embryos from cycles with a high
implantation rate WARRANTS
FURTHER RESEARCH on the origin
and significance of chromosomal
abnormalities in human
preimplantation embryos.