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Microscopes
The First Microscope
• The first microscope was created by
Zacharias Janssen
• For millennia, the smallest thing humans
could see was about as wide as a human
hair. When the microscope was invented
around 1590, suddenly we saw a new world
of living things in our water, in our food and
under our nose.
The Compound Light Microscope
• Light passes through the specimen
• Lenses enlarge or magnify the image
• Micrometers (μm) are used to measure very
tiny objects (1mm = 1000 μm)
Parts of the Microscope
Lowest magnification to highest
How to use a Microscope
Estimating Measurements
• Being able to estimate the size of an
object viewed under a microscope is
an important skill. It’s important to
understand Field ofView
• Field ofView: Field of view is the
diameter of the circular region of the
slide visible under the microscope
Estimating Measurements
• What will happen to the Field ofView
as we increase the magnification?
Why?
• As you increase the magnification the
FOV gets smaller (decreases).
Estimating Measurements
• If you knew the Field ofView (400 μm) of a
microscope, how could you estimate the
size of an object?
• You can divide the FOV by the number of
objects that would fit.
• Estimated size = FOV/#fit
• = 400μm/4
• =100μm
• 1mm = 1000μm
• So 100μm = 0.1 mm

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Microscopes

  • 2. The First Microscope • The first microscope was created by Zacharias Janssen • For millennia, the smallest thing humans could see was about as wide as a human hair. When the microscope was invented around 1590, suddenly we saw a new world of living things in our water, in our food and under our nose.
  • 3. The Compound Light Microscope • Light passes through the specimen • Lenses enlarge or magnify the image • Micrometers (μm) are used to measure very tiny objects (1mm = 1000 μm)
  • 4. Parts of the Microscope Lowest magnification to highest
  • 5. How to use a Microscope
  • 6. Estimating Measurements • Being able to estimate the size of an object viewed under a microscope is an important skill. It’s important to understand Field ofView • Field ofView: Field of view is the diameter of the circular region of the slide visible under the microscope
  • 7. Estimating Measurements • What will happen to the Field ofView as we increase the magnification? Why? • As you increase the magnification the FOV gets smaller (decreases).
  • 8. Estimating Measurements • If you knew the Field ofView (400 μm) of a microscope, how could you estimate the size of an object? • You can divide the FOV by the number of objects that would fit. • Estimated size = FOV/#fit • = 400μm/4 • =100μm • 1mm = 1000μm • So 100μm = 0.1 mm