Maxam gilbert method for DNA Sequencing

The Maxam-Gilbert method for DNA sequencing involves breaking DNA at specific bases using chemical agents, resulting in a nested set of radioactive fragments. These fragments are resolved through polyacrylamide gel electrophoresis, allowing for the determination of base sequence from the bands produced. Key steps include partial cleavage at adenine, guanine, cytosine, and thymine, with varying rates of cleavage and visualization through autoradiography.