This is a practical session during vaccine and serum course. A course for the fourth year undergraduate students (Pharmacology domain) at department of virology, faculty of veterinary medicine, Cairo University.

1. Antigen
selection and
preparation

2. Vaccinology
Industrial
vaccinology
Clinical
vaccinology
Traditional
empirical
vaccinology
Last 30 years
new
technology
dependent
vaccinology
Recombinant DNA vaccine
Glycoconjugation vaccine
Reverse vaccine
Next generation Vaccine
Subunit vaccine
Killed inactivated vaccine
Live attenuated vaccine
Synthetic biologics/RNA
vaccine
Adjuvant vaccine
Structural vaccine
The mother
of
vaccinology
is the
Natural
immunity
Toxoid vaccine

3. Types of Antisera
Sera of Animal origin Sera of Human origin
Venom Antiserum
(Anivenoms)
Bacterial Toxins
Antiserum
(Antitoxins)
Virus Antiserum
(Antiviruses)
Normal
immunoglobulins
Specific
Immunoglobulins
Botulinum
antitoxin
Diphtheria
antitoxin
Gas
gangrene
antitoxin
Tetanus
antitoxin
Polyvalent
IgG
Hepatitis
A
antisera
Hepatitis
B
Antisera
Measles
Antisera
Anti
D(RH)
Antisera
Anti
T-lymphocytes
Antisera

4. Both vaccine and
antisera
preparation needs
antigen
preparation

5. But before
Antigen
preparation
you should
know what is
the meaning
of Antigen ?
and how you
can select the
proper one ?

6. Antigen Hapten Immunogen Epitope
Any
foreign/enemy
substance
A small
molecular
weight
antigen
An immune
stimulating
antigen
A specific
piece of
antigen that
interact
specifically
with
antibody

7. Don’t forget to
search for the
“protective
antigen”

11. Through the following we will
discuss the most important
examples for Antigen selection
and preparation

12. Inactivated
vaccine
(Bacterin)
Pasteurella
multocida

13. Inactivation by 0.5% formaldehyde
Serological typing
Cultivation of seed strain
Bacterial count
Pure isolation and identification

14. Toxoid
vaccine
Clostridium spps.
Corynebacterium
diphtheriae

15. Preparation of special toxoid
preparation media
Cultivation of seed strain
lypholization
LD50 determination
Pure isolation and identification

16. Centrifugation
Pure isolation and identification
Ultracentrifugation
Blending with one selected from
three adjuvants categories

17. Live attenuated
vaccine
Mycobacterium
Listeria
monocytogens
Brucella
abortus

18. 45/20 vs strain19 vs RB 51