High-performance liquid chromatography (HPLC) is an analytical technique used to separate, identify, and quantify components in a mixture. It works by pumping a sample mixture through a column at high pressure using a solvent. Components are separated into bands as they interact differently with the stationary and mobile phases of the column on their way through. HPLC can separate and identify compounds present in trace concentrations and is widely used in applications like analyzing food products, environmental samples, medical products, and more due to its accuracy, affordability, and ability to quickly obtain high resolution results.
Content include basic introduction to chromatography. Brief view of Liquid Chromatography. HPLC introduction, other names, types of HPLC, detailed instrumentation with image of each part, and applications. Sources of content described in 'References' entitled slide. This presentation was prepared for the partial fulfillment of Master of Pharmacy.
Content include basic introduction to chromatography. Brief view of Liquid Chromatography. HPLC introduction, other names, types of HPLC, detailed instrumentation with image of each part, and applications. Sources of content described in 'References' entitled slide. This presentation was prepared for the partial fulfillment of Master of Pharmacy.
Chromatography- Principles and application of chromatographySanchit Dhankhar
Laboratory technique for the Separation of mixtures
Chroma -"color" and graphein - "to write”.
Colour bands - separation of individual compounds
Measured or analysed.
Analytical
Determine Chemical composition of a sample
Preparative
Used to purify sufficient quantities of a substance
Chromatograph - equipment that enables a sophisticated
separation
EX. Gas chromatography or Liquid chromatography
Eluent - Fluid entering column/ solvent that carries the analyte.
Eluate - Mobile phase leaving the column.
Stationary phase - Immobilized phase
Immobilized on the support particles or on the inner wall of the column tubing.
Examples : Silica layer - Thin Layer Chromatography
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate
Chromatography- Principles and application of chromatographySanchit Dhankhar
Laboratory technique for the Separation of mixtures
Chroma -"color" and graphein - "to write”.
Colour bands - separation of individual compounds
Measured or analysed.
Analytical
Determine Chemical composition of a sample
Preparative
Used to purify sufficient quantities of a substance
Chromatograph - equipment that enables a sophisticated
separation
EX. Gas chromatography or Liquid chromatography
Eluent - Fluid entering column/ solvent that carries the analyte.
Eluate - Mobile phase leaving the column.
Stationary phase - Immobilized phase
Immobilized on the support particles or on the inner wall of the column tubing.
Examples : Silica layer - Thin Layer Chromatography
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate
Chromatography is the most widely accepted and important technique in the organic chemistry for the separation, purification, identification and characterization of components of a mixture.
Pharmacy practice in research and developmentShariah Brishti
Importance of R&D
Crucial to survival
Fast changing environment
Continuous technology change
To the development of new products
Changing consumer preferences
To improvement of existing products or procedures
Understanding what markets need (MR)
Efficient production processes
AI and Machine Learning Demystified by Carol Smith at Midwest UX 2017Carol Smith
What is machine learning? Is UX relevant in the age of artificial intelligence (AI)? How can I take advantage of cognitive computing? Get answers to these questions and learn about the implications for your work in this session. Carol will help you understand at a basic level how these systems are built and what is required to get insights from them. Carol will present examples of how machine learning is already being used and explore the ethical challenges inherent in creating AI. You will walk away with an awareness of the weaknesses of AI and the knowledge of how these systems work.
An introduction to HPLC(High Performance LIquid Chromatography) was depicted in the presentation.
Simultaneously, the each and every component of HPLC was explained by depicting with a diagram in the slide.
The key notes are also included in the presentation.
Instrumentation of HPLC, principle by kk sahuKAUSHAL SAHU
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
A brief review on development and validation of hplc method.adhirajain
the slides in the ppt gives a brief review on product development and its validation in HPLC method. Contents are with advantages, disadvantages, application , classification and methods for development.
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
This is a PPT for HPLC which I made for presenting my assigned topic for Practice School during 7 sem of my graduation . Hope it is useful for you guys :)
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2. High-performance liquid chromatography (HPLC) is an analytical technique
to separate, identify, and quantify components in a mixture. It is the single biggest
chromatography technique essential to most laboratories worldwide.
INTRODUCTION
3. • Biogenic substances
Sugars, lipids, nucleic acids, amino acids,
proteins , peptides, steroids, amines, etc.
• Medical products
Drugs, antibiotics, etc.
• Clinical Tests
Urine analysis, antibiotics analysis in
blood. bilirubin, biliverdin analysis in
hepatic disorders.
APPLICATION OF HPLC
4. Food products
Vitamins, food additives, sugars, organic
acids, amino acids, etc.
Environmental samples
Inorganic ions
Hazardous organic substances, etc.
Organic industrial products
Synthetic polymers, additives, surfactants,
etc.
APPLICATION OF HPLC….
5. • High Performance Liquid Chromatography (HPLC) is a form of
column chromatography that pumps a sample mixture in a solvent
at high pressure through a column with chromatographic packing
material.
• All chromatographic separations, including HPLC operate under
the same basic principle; separation of a sample into its
constituent parts because of the difference in the relative affinities
of different molecules for the mobile phase and the stationary
phase used in the separation.
PRINCIPLE OF HPLC
6. • HPLC has the ability to separate, and identify compounds that
are present in any sample that can be dissolved in a liquid in
trace concentrations as low as parts per trillion.
• As the sample passes through the column it interacts between
the two phases at different rate, primarily due to different
polarities in the analytes.
• Analytes that have the least amount of interaction with the
stationary phase or the most amount of interaction with the
mobile phase will exit the column faster.
PRINCIPLS OF HPLC….
8. Solvent Reservoir: A reservoir holds the solvent called the mobile phase.
Pump : A high-pressure pump aspirates the mobile phase from the solvent reservoir
and forces it through the system’s column and detector.
Sample Injector : An injector is able to introduce the sample into the continuously
flowing mobile phase stream that carries the sample into the HPLC column.
How does it work…..
9. Columns : The column contains the chromatographic packing material needed to
effect the separation.
Detector : A detector is needed to see the separated compound bands as they
elute from the HPLC column.
Data Collection Devices :The computer integrates the response of the detector
to each component and places it into a chromatograph that is easy to read and
interpret.
How does it work…..
10. • It is a Powerful , adaptable and automated
process.
• results obtained quickly and easily.
• High resolution results are obtained.
• Affordable.
• Accurate results.
• Quick method for analysis of sample.
ADVANTAGES OF HPLC
