An introduction to HPLC(High Performance LIquid Chromatography) was depicted in the presentation.
Simultaneously, the each and every component of HPLC was explained by depicting with a diagram in the slide.
The key notes are also included in the presentation.
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
A brief introduction and instrumentation on high-performance liquid chromatography which is widely used in the analytical analysis of different samples in the pharmacy field. In this presentation, there is a small case study in finding out the different mobile and stationary phases used in the HPLC analysis.
APPLICATIONS OF HPTLC IN DETERMINATION OF BIOANALYTICAL BASED SEPARATIONPHARMA IQ EDUCATION
APPLICATIONS OF HPTLC IN FIELD OF MEDICAL AND PHARMACEUTICAL SCIENCES WHICH COVERS IN SHORT ABOUT THE BIOANALYSIS OF DRUGS, LIPIDS, AMINO ACIDS, CARBOHYDRATES, PORPHYRINS etc.
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
A brief introduction and instrumentation on high-performance liquid chromatography which is widely used in the analytical analysis of different samples in the pharmacy field. In this presentation, there is a small case study in finding out the different mobile and stationary phases used in the HPLC analysis.
APPLICATIONS OF HPTLC IN DETERMINATION OF BIOANALYTICAL BASED SEPARATIONPHARMA IQ EDUCATION
APPLICATIONS OF HPTLC IN FIELD OF MEDICAL AND PHARMACEUTICAL SCIENCES WHICH COVERS IN SHORT ABOUT THE BIOANALYSIS OF DRUGS, LIPIDS, AMINO ACIDS, CARBOHYDRATES, PORPHYRINS etc.
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).
All you need to know about the HPLC Chromatography!
HPLC (High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture. HPLC separates and purifies compounds according to their polarity. I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.
I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences. Watch this video for more details.
If you have any doubts: Please leave a comment in the comments section below.
Instrumentation of HPLC, principle by kk sahuKAUSHAL SAHU
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
Content include basic introduction to chromatography. Brief view of Liquid Chromatography. HPLC introduction, other names, types of HPLC, detailed instrumentation with image of each part, and applications. Sources of content described in 'References' entitled slide. This presentation was prepared for the partial fulfillment of Master of Pharmacy.
Applications, uses of 5G but also a summary of all generations of Networks we had till date.
This presentation helps to attain Konwledge, and also to get prepared for the upcoming competetive exams as well.
Exam bits related to 5G may appear in the exams under the section of Current affairs.
This Presentation helps the Studetns preparing for the CSIR-NET in Life Sciences. Not only CSIR but also the exams like DBT, GATE, ICMR, TIFR and all the other exams related to Life Sciences.
For MSc entrance exams too.
This topic deals with the Plant Metabolic process. It is under the subject Plant Physiology.
The slides shown in this PPT are very important for all the aspirants preparing for LIfe Sciences Exams like CSIR-NET, DBT-JRF, GATE, ICMR etc..
The points and MCQs depicted in this video are previously appeared in the above said exams.
This Presentation are very helpful for Lecturers to Teach and Students to Learn.
The points depicted in this presentation are very helpful for the aspirants like CSIR-NET, DBT-JRF, GATE, ICMR, TIFR etc.,
These points (some Question and Answers) are helpful for Life Sciences exams.
The tricks shown in every slide are must to remember and hence "Points to Remmeber"!!
This presentation gives the clear details on the Mammals. Information on all type of Mammals are included.
Fast and quick facts are also included. The pictures are neatly depicted by which one can understand easily.
Gestation period of Mammals are depicted in an order. Some rarely observed Mammals are shown in the initial slides.
Parents and Teachers find this presentation very helpful as this is very easy to explain their Children. Even, Civil Service Exam aspirants and other exam aspirants like Life Sciences, Groups etc., find this presentation helpful and useful for their preparation.
Quick facts 3! #StayHome and Save Lives!Bharath Raj
The facts depicted in this PPT are very useful in gaining knowledge and awareness. These are very helpful for Children to learn and even Adults also to Teach their Children or Students and ask them during leisure time.
Learning these also helps in getting prepared for competetive examinations. In Civil Services Interview, question like these are asked.
Learn well, have fun and Enjoy!!
#StayHome and help save lives #WithMe
The facts presented in this PowerPoint Presentation are interesting to learn and increases one's enthusiasm. These are very helpful for Children as well as Adults or Parents. Parents can Teach their children during leisure time holidays of lock-downtime.
In this way Parents can help their Children in gaining General Knowledge by discussing these facts!
The facts shown in this PPT are very interesting and knowledge oriented. Images are also depicted to have an easy understanding. Helpful for the Children in their School projects.
Even for Parents also. During the leisure time, you can teach these facts your Children. Imparts knowledge.
The Earth is the common Mother to all of us. She withstood many difficulties, deformations, damages and is making our Life full of Happies. As the Children, it is our responsibility to take care of Her properly and provide Her with good Health.
In this PPT, the importance of our Mother Earth and a brief History has been presented. Read it, Learn it, Enjoy it and Excel!!
She helped man people to overcome the AIDS. She is the Torch-bearer in the field of Virology. A Renowned Scientist in Life Sciences. Dr. Geeta Ramjee Madam is an Angel God for many who are being suffered with HIV-AIDS.
She introduced technical solutions to eradicate the AIDS from the society and Her research works played an amazing role in lighting up the lamp in the lives of AIDS patients instead of darkness.
The facts shown in this PPT are very interesting to learn and know. The images are also depicted for each fact so as to make the viewer understand well. The General Knowledge as well as your timee gets passed easily during this lock-down time. #StayHome #StaySafe
At present situation, the COVID-19 outbreak is disappointing every individual, every Society, every Nation and the whole World.
The necessity of Testing kits to check for the Virus among the people is need of the hour.
In this presentation, the testing kits, their types, working mechanisms, working Principle are explained in a detailed manner. Kindly go through all the slides.
The full-forms and abbreviations given in this PPT are very helpful for all the competitive examination of Life Sciences like CSIR, DBT, GATE,ICMR, NEET, AIMS etc..
The Enzymes present in our Human body are explained in this PPT. The roles of the various enzymes, their location, secreted by etc.. have been depicted. The images are also given. The digestive enzymes like Pepsin, Rennin etc.. are clearly mentioned.
The points are essential for the aspirants who are preparing for the competitive examinations (including Medical and non-medical exams).
The precautions and care to be taken to avoid the Viral infection is depicted in this PPT. Almost all the precautions are generally taught by our parents in our home like "cut your nails, wash your hands whenever you touch hair, dust off the clothes outside the house" etc...
{March 22nd is observed as the World Water Day. Ned to conserve Water, otherwise there will be no Water at least for cleaning the hands and to eliminate virus from the society. The theme for 2020 is "Water and Climate Change" i.e the link or relation between Water and Climate change. the theme for 2019 is "Leaving No One behind"}.
The points depicted in this video are so important for the aspirants of Life Sciences exams like GATE, CSIR-NET, DBT-JRF, ICAR, MSc entrance exams etc.,
These points have been asked in various above said exams previously.
Each and every slide contains very important points for the aspirants of Life Sciences examinations. Even for MSc entrance exams also these questions are very helpful in getting prepared for the exam!!
The slides depicted in this presentation carries the General knowledge bits which are very important for the competitive exam preparation.
The aspirants are advised to go through this presentation and gain knowledge. Not only for competitive exam preparation but also for children this is very helpful. Parents can ask these bits to their children randomly in random time!!
The important points on DNA Polymerase I are exclusively depicted in this Presentation. The roles and functions of DNA Pol I during the Molecular processes are discussed.
{Every year, January 24th is observed as the National Girl Child. It is the responsibility of everyone of us to bring awareness about the Girl child. Every Girl must be given proper Nutrition, proper Education, proper encouragement in the studies, Sports and Cultural activities. One is not supposed to confine the Girl to a Kitchen room. It is the right of every Girl child to get educated and become independent.
Dr. APJ Abdul Kalam Sir says, "Education is the most powerful weapon which you can use to change the world". Like that, every Girl must be educated and given a 'weapon'.}
{Disclaimer: The images have been downloaded from Google images and used in this video. I thank all the sources, references, and creators of these images. These pictures are used for EDUCATION and fair purpose only}
All the points depicted in this presentation are very important and asked in the competitive exams like CSIR, GATE and various University entrance examinations previously.
So, don't neglect these points, make a note of it and revise.
Hope you like my presentation. Thank you.
Quality defects in TMT Bars, Possible causes and Potential Solutions.PrashantGoswami42
Maintaining high-quality standards in the production of TMT bars is crucial for ensuring structural integrity in construction. Addressing common defects through careful monitoring, standardized processes, and advanced technology can significantly improve the quality of TMT bars. Continuous training and adherence to quality control measures will also play a pivotal role in minimizing these defects.
Courier management system project report.pdfKamal Acharya
It is now-a-days very important for the people to send or receive articles like imported furniture, electronic items, gifts, business goods and the like. People depend vastly on different transport systems which mostly use the manual way of receiving and delivering the articles. There is no way to track the articles till they are received and there is no way to let the customer know what happened in transit, once he booked some articles. In such a situation, we need a system which completely computerizes the cargo activities including time to time tracking of the articles sent. This need is fulfilled by Courier Management System software which is online software for the cargo management people that enables them to receive the goods from a source and send them to a required destination and track their status from time to time.
Saudi Arabia stands as a titan in the global energy landscape, renowned for its abundant oil and gas resources. It's the largest exporter of petroleum and holds some of the world's most significant reserves. Let's delve into the top 10 oil and gas projects shaping Saudi Arabia's energy future in 2024.
Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
COLLEGE BUS MANAGEMENT SYSTEM PROJECT REPORT.pdfKamal Acharya
The College Bus Management system is completely developed by Visual Basic .NET Version. The application is connect with most secured database language MS SQL Server. The application is develop by using best combination of front-end and back-end languages. The application is totally design like flat user interface. This flat user interface is more attractive user interface in 2017. The application is gives more important to the system functionality. The application is to manage the student’s details, driver’s details, bus details, bus route details, bus fees details and more. The application has only one unit for admin. The admin can manage the entire application. The admin can login into the application by using username and password of the admin. The application is develop for big and small colleges. It is more user friendly for non-computer person. Even they can easily learn how to manage the application within hours. The application is more secure by the admin. The system will give an effective output for the VB.Net and SQL Server given as input to the system. The compiled java program given as input to the system, after scanning the program will generate different reports. The application generates the report for users. The admin can view and download the report of the data. The application deliver the excel format reports. Because, excel formatted reports is very easy to understand the income and expense of the college bus. This application is mainly develop for windows operating system users. In 2017, 73% of people enterprises are using windows operating system. So the application will easily install for all the windows operating system users. The application-developed size is very low. The application consumes very low space in disk. Therefore, the user can allocate very minimum local disk space for this application.
Overview of the fundamental roles in Hydropower generation and the components involved in wider Electrical Engineering.
This paper presents the design and construction of hydroelectric dams from the hydrologist’s survey of the valley before construction, all aspects and involved disciplines, fluid dynamics, structural engineering, generation and mains frequency regulation to the very transmission of power through the network in the United Kingdom.
Author: Robbie Edward Sayers
Collaborators and co editors: Charlie Sims and Connor Healey.
(C) 2024 Robbie E. Sayers
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
2. INTRODUCTION:
High Performance Liquid Chromatography is a powerful tool in
analytical Chemistry used to segregate, identify and quantify each
component in a mixture.
It relies on pumps to pass a pressurized liquid solvent containing the
sample through a column filled with adsorbent material.
3. Solvent
Pump Mobile phase Stationary Phase
Waste or
collecting
tube
Detector
Diag: Block diagram of HPLC
Sample Injector
Degasser
System
4. Solvent Reservoir:
• Solvents must be highly
pure.
• 2 types of reservoirs are
there:
• Binary system
• Quaternary system
5. Degasser
• Removes off the gases
from solvents.
• These gases are nothing
but impurities which
stops the proper
segregation and
quantification process.
6. Pump:
• Pump is an essential
component in HPLC and
making the mobile phase to
mobilise.
• The high pressure in HPLC
is maintained by the Pump
itself.
• Appprx. 400 atmospheres of
pressure is induced!!
7. Mobile Phase
• Mobile phase =(90% of Water)
+ (10% of Organic solvent.)
• Hydrophilic in nature (since
high amount of Water is
present).
• Usually, mobile phase is the
congregation of Water +
Acetonitrile and /or Methanol
8. Pre-column/Guard
column
• Protects the Analytical column
• Any contamination in the
solvent is purified at this step.
• Size of this column is lesser
than the Analytical column.
• Conditioning of solvent occurs
here.
9. Sample Injector
• The sample whose components
are to be verified is injected
through the Sample Injector.
10. Stationary Phase
• Analysis of Sample like segregation,
quantification occurs in the
Stationary phase.
• This is Hydrophobic in nature.
• It is an adsorbent and adsorbs the
sample based on polarity.
• Ex: C-18 columns,
C-8 Columns
Silica columns
11. Detectors:
• This component detects the analyte and depicts the
magnitude in the screen.
• Various types:
• UV-Vis Spectrophotometer
• Mass detector
• Concentration detector
• Conductivity detector
• Conductivity detector
• Refractive Index detector
• Fluoresence detector
• Amperometric detector
12. PROCEDURE:
• The Solvent from reservoir is pumped out through the mobile phase and reaches the stationary phase.
• The separation process occurs in Stationary phase by the means of Polarity.
• Polarity means Hydrophobic and Hydrophilic nature.
• Sample means the Blood, Fungal toxins, Plant material etc.. employed for purification.
• Mobile phase + Sample are passing through the Stationary phase.
13. • The hydrophilic components(in sample) are easily passes through the analytical column and a
primary peak gets generated in the system.
• Whereas the hydrophobic components forms a bond with the hydrophobic nature of stationary
phase.
• As the pressure increases, breakage of bond occurs and hence a secondary peak with different
reading is recorded in the system.
• In this way the separation, quantification and identification occurs and chromatogram is
analysed.
