This document provides an overview of high performance liquid chromatography (HPLC). It discusses the types of HPLC including normal phase, reversed phase, size exclusion, and ion exchange chromatography. The key components of an HPLC instrumentation are described including the solvent reservoir, pump, injector, columns, detector, recorder, degasser, and data collection system. Applications of HPLC are in fields like pharmacy, forensics, environment, and clinical analysis for separation and purification of compounds. Advantages of HPLC include speed, efficiency, accuracy, and versatility in identifying and quantifying chemical components.