Tanu Gabhane's presentation discusses High Performance Liquid Chromatography (HPLC). HPLC is an analytical chemistry technique used to separate, identify, and quantify components in a mixture. It works by pumping a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Components separate based on their affinity for the stationary phase, with those having more affinity moving slower through the column. HPLC has various applications in forensics like identifying anabolic steroids, analyzing textile dyes, and determining drugs and metabolites in samples.
High Performance Liquid Chromatography HPLC is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent mobile phase flowing through a column packed with a separation medium stationary phase . Sample components separate from one another by a process of differential migration as they flow through the column.As bands emerge from the column, flow carries them to one or more detectors which deliver a voltage response as a function of time. This is called a chromatogram. For each peak, the time at which it emerges identifies the sample constituent with respect to a standard. The peak’s area represents the quantity .HPLC provides a highly specific, reasonably precise, and fairly rapid analytical method for a plethora of complicated samples.This is difficult in detecting compounds. Low sensitivity of some compounds towards the stationary phase in the columns is difficult. Mohd Ali | Panjak Chasta | Dr. Kausal Kishore Chandrul "High Performance Liquid Chromatography (HPLC)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45146.pdf Paper URL: https://www.ijtsrd.com/pharmacy/other/45146/high-performance-liquid-chromatography-hplc/mohd-ali
Introduction points in High Performance Liquid Chromatography.
Principle
Theory of HPLC
Kinetic Theory (Van Deemter)
Factors affecting Column Performance
Capacity Factor
Selectivity Factor
Factors affecting Resolution
Mobile phase composition effect on separation in HPLC
Standard Addition Method
Internal Standard Method
Types of HPLC
-Prajakta
HPLC: Principle and Maintenance with Applicationijtsrd
High performance liquid chromatography (HPLC) is a significant qualitative and quantitative technique, usually used for the estimation of pharmaceutical and biological samples. The chromatography term is derived from the Greek words namely chroma (colour) and graphein (to write). The chromatography is very accepted technique and it is mostly used analytically. It is the most resourceful, safest, reliable and fastest chromatographic technique for the quality control of drug components. This technique involves 2 phases"™ stationary and mobile phases. There are different types of chromatographic techniques. The separation of constituents is based on the variation between the partition coefficients of the two phases. This article is primed with an aim to review different aspects of HPLC, such as principle, types, instrumentation and application with maintenance. Yogesh Kumar | Sayed Md Mumtaz | Mustaq Ahmad"HPLC: Principle and Maintenance with Application" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd17134.pdf http://www.ijtsrd.com/pharmacy/pharmacology-/17134/hplc-principle-and-maintenance-with-application/yogesh-kumar
RP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmac...Sunil Vadithya
RP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmaceutical Dosage FormRP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmaceutical Dosage Form
HPLC
Chromatography
Mobile Phase & Stationary Phase
CLASSIFICATION OF CHROMATOGRAPHY
Characteristics of HPLC
Purpose
Superiority of HPLC
TYPES OF HPLC TECHNIQYES
Principle
PHASING SYSTEM & (normal vs reversed phase)
INSTRUMENTATION
Flow diagram of HPLC instrument
Advantages of HPLC
High Performance Liquid Chromatography HPLC is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent mobile phase flowing through a column packed with a separation medium stationary phase . Sample components separate from one another by a process of differential migration as they flow through the column.As bands emerge from the column, flow carries them to one or more detectors which deliver a voltage response as a function of time. This is called a chromatogram. For each peak, the time at which it emerges identifies the sample constituent with respect to a standard. The peak’s area represents the quantity .HPLC provides a highly specific, reasonably precise, and fairly rapid analytical method for a plethora of complicated samples.This is difficult in detecting compounds. Low sensitivity of some compounds towards the stationary phase in the columns is difficult. Mohd Ali | Panjak Chasta | Dr. Kausal Kishore Chandrul "High Performance Liquid Chromatography (HPLC)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45146.pdf Paper URL: https://www.ijtsrd.com/pharmacy/other/45146/high-performance-liquid-chromatography-hplc/mohd-ali
Introduction points in High Performance Liquid Chromatography.
Principle
Theory of HPLC
Kinetic Theory (Van Deemter)
Factors affecting Column Performance
Capacity Factor
Selectivity Factor
Factors affecting Resolution
Mobile phase composition effect on separation in HPLC
Standard Addition Method
Internal Standard Method
Types of HPLC
-Prajakta
HPLC: Principle and Maintenance with Applicationijtsrd
High performance liquid chromatography (HPLC) is a significant qualitative and quantitative technique, usually used for the estimation of pharmaceutical and biological samples. The chromatography term is derived from the Greek words namely chroma (colour) and graphein (to write). The chromatography is very accepted technique and it is mostly used analytically. It is the most resourceful, safest, reliable and fastest chromatographic technique for the quality control of drug components. This technique involves 2 phases"™ stationary and mobile phases. There are different types of chromatographic techniques. The separation of constituents is based on the variation between the partition coefficients of the two phases. This article is primed with an aim to review different aspects of HPLC, such as principle, types, instrumentation and application with maintenance. Yogesh Kumar | Sayed Md Mumtaz | Mustaq Ahmad"HPLC: Principle and Maintenance with Application" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd17134.pdf http://www.ijtsrd.com/pharmacy/pharmacology-/17134/hplc-principle-and-maintenance-with-application/yogesh-kumar
RP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmac...Sunil Vadithya
RP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmaceutical Dosage FormRP-HPLC Method Development and Validation of Ketoconazole in Bulk and Pharmaceutical Dosage Form
HPLC
Chromatography
Mobile Phase & Stationary Phase
CLASSIFICATION OF CHROMATOGRAPHY
Characteristics of HPLC
Purpose
Superiority of HPLC
TYPES OF HPLC TECHNIQYES
Principle
PHASING SYSTEM & (normal vs reversed phase)
INSTRUMENTATION
Flow diagram of HPLC instrument
Advantages of HPLC
Instrumentation of HPLC, principle by kk sahuKAUSHAL SAHU
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
An introduction to HPLC(High Performance LIquid Chromatography) was depicted in the presentation.
Simultaneously, the each and every component of HPLC was explained by depicting with a diagram in the slide.
The key notes are also included in the presentation.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
2. Content:
• HPLC
• Principle of HPlC
• Instrumentation
• Working
• Types of HPLC
• Forensic significance
High
Performance
Liquid
Chromatograph
y
3. – High performance liquid chromatography (HPLC)
(HPLC), formerly referred to as high-pressure liquid
chromatography,
It is a technique in analytical chemistry used to separate,
identify, and quantify each component in a mixture.
It relies on pumps to pass a pressurized liquid solvent
containing the sample mixture through a column filled with a
solid adsorbent material.
4. Principle :
The separation of hplc is to force the sample through the column of
the stationary phase by pumping the mobile phase at high pressure
.
Compounds are separated by Injecting a sample mixture into the
column. The different component in the Mixture pass through the
column and differentiates according to their affinity towards the
stationary phase.
* More affinity towards stationary phase moves slower
* Less affinity towards the stationary phase moves faster.
8. Types of HPLC:
1. Normal phase HPLC : Stationary phase is polar
and mobile phase is non polar.
2. Reverse phase HPLC :Stationary phase is non
polar and mobile phase is polar.It is mostly used
because most of the solvent are organic solvent.
9. Forensic significance:
• Identification of anabolic steroids in
serum,urine,sweat and hair.
• Analysis of textile dyes.
• Determination of cocaine and metabolites
in sample
• Quantification of drugs In biological
samples,etc
