Guidelines for the Conduct of Tests for Distinctiveness, Uniformity and Stability On
Chilli (Hot Pepper), Bell (Sweet) Pepper and Paprika(Capsicum annuum L.)
Breeding technology of Okra
vsc - 503
Okra is a seed propagated hot weather crop sensitive to frost, low temperature, and waterlogging as well as drought conditions. It is a multipurpose crop due its various uses. It is grown in many countries and cultivars from different countries have certain adapted distinguishing characteristics specific to the country to which they belong. In home consumption India tops the world. Genetic diversity exists for number of characters. Genetic resistance involving interspecific crosses have been exploited commercially for Yellow Vein Mosaic virus. Hybrids are very much popular in this crop and the hybrid seed production is based on hand emasculation and hand pollination. Proper isolation is necessary between two fields of two varieties for maintaining the genetic purity of the stock.
Breeding technology of Okra
vsc - 503
Okra is a seed propagated hot weather crop sensitive to frost, low temperature, and waterlogging as well as drought conditions. It is a multipurpose crop due its various uses. It is grown in many countries and cultivars from different countries have certain adapted distinguishing characteristics specific to the country to which they belong. In home consumption India tops the world. Genetic diversity exists for number of characters. Genetic resistance involving interspecific crosses have been exploited commercially for Yellow Vein Mosaic virus. Hybrids are very much popular in this crop and the hybrid seed production is based on hand emasculation and hand pollination. Proper isolation is necessary between two fields of two varieties for maintaining the genetic purity of the stock.
“Advances in breeding of mango ”
Advances breeding of Mango, breeding of mango, mutation breeding og mango ,breeding of mango by gangaram rana ppt , breeding of mango in igkv
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
“Advances in breeding of mango ”
Advances breeding of Mango, breeding of mango, mutation breeding og mango ,breeding of mango by gangaram rana ppt , breeding of mango in igkv
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
Seed marks the beginning of each plant production and therefore
ensuring its quality is the priority of modern seed science and a prerequisite
for obtaining high yields of all plant species. Determination of seed quality
and its viability indicates what seed lots can be placed onto the market, and
for that reason it is very important to have reliable methods and tests to be
used for seed quality and seed vigour testing
Study on Genetic Variability, Heritability, Genetic Advance and Correlation a...IJEABJ
The present investigation entitled “Studies on genetic variability in tomato (Solanum lycopersicum L.)” was carried out at the UCOA, vegetable research farm, Guru Kashi University, Talwandi Sabo, Bathinda during rabi 2015-16 to evaluate tomato genotypes. The experiment was laid out in CRD with three replications. Total 20 genotypes including check cultivar were evaluated for horticultural Traits contributing yield and quality (suitable for processing) .There is a wide variability in different genotypes in tomato. Traits i.e. Number of primary branches per plant, Days to first fruit harvest, Plant height (cm),number of fruits per cluster, number fruits per plant, average fruit weight (gm), equatorial diameter of fruit (cm), polar diameter of fruit (cm), number of locules per fruit, pericarp thickness (mm), fruit pH, Fruit TSS (0brix), days to last fruit harvest and average yield per plant (kg) were studied during the investigation Analysis of variance showed significant differences among genotypes for all the characters under study during the investigation. High Phenotypic and Genotypic coefficient of variation were detected for characters like number of fruits per plant, number of locules per fruit, pericarp thickness and average yield per plant. High heritability coupled with genetic gain were recorded for number of fruits per plant, average fruit weight, number of locules per fruit and average yield per plant. Therefore these characters also show some scope for improvement through selection. A highly significant and positive phenotypic and genotypic correlation were found in number of fruits per cluster, plant height, number of fruits per plant and average fruit weight.
B4FA 2013 Ghana: Cassava mosaic disease resistance - Paul Asareb4fa
Presentation at the March 2013 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Accra, Ghana.
Please see www.sti4d.com/b4fa for more information
Stable diagnostic characters of parental lines and their hybrids of cotton, c...Yanal Al-Kuddsi
To achieve desired impact of high yielding varieties and improved production technology, availability of high quality of seed is the first and foremost requirement.
Glimpses of ongoing research on Papaya cultivation on chitwanagriyouthnepal
This is the presentation given by Mr. Santosh Kalauni on " AYN Friday Sharing" program on 29- July-2016. The presentation was based on the research that is being carried out the Red lady variety of papaya. Mr. Kalauni is a student of MSc. Ag (Hort) at AFU, Rampur.
DOI: 10.21276/ijlssr.2016.2.3.1
ABSTRACT- Genetic variability studied for eleven characters viz., number of leaves, branches, clusters, pods, pod
length (cm), seed per plant, plant height (cm), 100 seed weight (g), days to flower, maturity and yield per plant (g) in
group of forty two genotypes of French bean (Phaseolus vulgaris L.) revealed highly significant difference for all the
characters. High heritability not only guideline for breeding improvement must to associated with genetic advance
expressed a wide range of variability for all the traits. Heritability estimated and expected genetic advance were high
for number of leaves (95.46< 97.00), branches (45.85 > 14.00), clusters (89.45< 92.00), pods (92.84< 99.00), pod length
(88.94< 99.00), seeds per plant (92.20> 32.00), plant height (96.41< 106.00), 100 seed weight (99.22> 72.00), days to
flower (94.82 > 32.00), maturity (56.26> 13.00) and yield per plant (80.13 < 140.00) suggesting that these are more
useful traits for varietal improvement programme.
Key-words- French bean (Phaseolus vulgaris L.), Genetic Variability, Heritability, Phenotypic coefficient of variation,
Genotypic coefficient of variation
Genetic and Morphological Diversity Analysis of Ethiopian Mustard (Brasica ca...Premier Publishers
Growing of Ethiopian mustard, as an oilseed and leaf vegetable require genetic improvement which relies on its genetic diversity. The experiment was executed to analyse genetic and morphological diversity of Ethiopian mustard land races at Holetta agricultural research center. Forty-nine genotypes collected from different agro ecologies were analyzed using morphological traits in order to assess the genetic and morphological diversity that exists in these materials. The experiment was carried out in a simple lattice design. The analysis of variance showed that there were significant differences among genotypes for all traits compared except seed yield per plant, fresh leaf biomass per plant topped at 40, 50 and 60 days of growth and number of intact leaves at flowering. The significant difference indicates the existence of genetic variability among the accessions that is important for selection and breeding. For yield component traits, high genotypic and phenotypic coefficient of variations was observed in seed yield per plot and oil yield. This shows that selection of these traits based on phenotype, may be useful for yield improvement. The highest heritability in broad sense was recorded for thousand seed weight(68.80%), followed by days to flowering (65.91%), stand percent (63.14%), linolenic acid (62.58%), days to maturity(60.43%), plant height (59.63%), palmitic (58.19%), linoleic (57.46%),leaf area (52..09%), oil content (50.33%), leaf width (48.29%),leaf length(46.28%), oil yield(44.84%), fresh leaf biomass at 50 days of topping(43.40%), seed yield per plot(42.99%), number of leaves at vegetative state(40.48%), seed yield of 50 days growth stage topped plants(38.85%) and primary branches (34.20%). This suggests that large proportion of the total variance was due to high genotypic variance. Hence, a good progress can be made if some of these traits are considered as selection criteria for the improvement of yield, yield component and vegetative traits. The present study revealed the presence of considerable variability among genotypes for all traits compared except seed yield per plant, fresh leaf biomass per plant topped at 40,50 and 60 days of growth and number of intact leaves at flowering. These conditions indicate that there is good opportunity to improve these characters using the tested genotypes.
Similar to Guidelines for the Conduct of Tests for DUS On Chilli (Hot Pepper), Bell (Sweet) Pepper and Paprika (20)
GUIDELINES FOR SENDING SEEDS TO NETWORK OF ACTIVE/WORKING COLLECTIONSkartoori sai santhosh
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Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
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We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
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The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
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Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Guidelines for the Conduct of Tests for DUS On Chilli (Hot Pepper), Bell (Sweet) Pepper and Paprika
1. Guidelines for the Conduct of Tests for Distinctiveness,
Uniformity and Stability
On
Chilli (Hot Pepper), Bell (Sweet) Pepper and
Paprika(CapsicumannuumL.)
KARTOORI SAISANTHOSH
2. Material required
• OPV-15g
• Hybrid and parents-10g
• Germination >85%
• Moisture content <8%
• Physical purity >98%
3. Test plot design
Bed size : 4.5 X 3.6m
Number of rows : 4
Row length : 4.5m
Row to row distance : 90cm
Plant to plant distance : 45cm
Number of replications : 3
Expected no. of plants : 40 X 3=120
4. Description Code
a) Cotyledons completely unfolded to two-true leaf stage 10
b) Active vegetative phase 20
c) Appearance of flowers on 50% plants 30
d) First fruit attaining harvest maturity 40
e) First fruit attaining physiological maturity 50
f)
Full maturity: approximately all fruits are shrunken, red/
yellow 60
Stages of recording observations on specific characteristics
5. Characteristics used for grouping Capsicm annuum
(a) Plant : Habit (characteristic 2)
(b) Flower/Fruit : Orientation (characteristic 24)
(c) Fruit : Fruit bearing habit (characteristic 25)
(d) Fruit : Colour (at mature green fruit stage on plants) (characteristic 26)
(e) Fruit : Shape in longitudinal section (characteristic 30)
(f) Fruit : Colour (at ripe fruit stage on plants) (characteristic 37)
(g) Fruit : Shape at base (characteristic 41)
(h) Fruit : Shape at apex (characteristic 42)
35. DUS test centres
Nodal DUS Centre Co-Nodal DUS Centre
Indian Institute of Horticultural Research
(IIHR),
Indian Agricultural Research Institute (IARI),
Pusa,
Hessarghatta Lake Post, Bengaluru - 560089 New Delhi -110012
CROP PUBLIC PRIVATE FARMER INDIVIDUAL
BREEDER
CHILLI 0 7 29 0
BELL PEPPER 0 0 4 0
STATUS OF APPLICATIONS FOR CHILLI AND BELL PEPPER 2007- 30.11.2015