This document summarizes a student's synopsis on studying genetic inheritance in sesame (Sesamum indicum L.). The student will study 21 sesame genotypes, including parents and F1 and F2 generations. Observations will be recorded on yield and quality traits. Statistical analyses will include variance components, heritability, genetic advance, correlation, and heterosis. Molecular characterization will involve DNA isolation, quantification, PCR amplification with markers, and data analysis. The overall goal is to understand genetic inheritance patterns in sesame.

1. Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.)
Synopsis Seminar
Session :-2019
Major Advisor:-
Mr.Roshan Parihar
Assistant Professor
Department of (Genetics & Plant
Breeding)
B.T.C. CARS, Bilaspur
Presented By:-
Dhanendra Dhanuka
M.Sc.(Ag.) Final Year
Department of (Genetics &
Plant Breeding)
B.T.C. CARS, Bilaspur
B.T.C. College of Agriculture & Research Station,
Bilaspur (C.G.)

2. Mr.Roshan Parihar
Major Advisor and Chairman
Members of Advisory committee
Dr. A.P.Agrawal ( Head of Section )
Dr. D.J.Sharma (Member from Department)
Dr.R.B.Tiwari (Member from other Department)
Dr. N. K. Chaure (Member from Supporting
Department)

3. Title of thesis problem
“To study about Genetic inheritance study
on sesame (Sesamum indicum L.) ”

4. Introduction
• Sesame, the Queen of oilseeds, occupies important
place in
• oilseed scenario of the country, next only to
groundnut and rapeseed in case of area, production
and productivity. Sesame botanically called as
Sesamum indicum L.
• belongs to order pedalineae and family
pedaliaceae,
• is a self pollinated crop. Chromosome no- 2n=26 It
is grown throughout the world in all the seasons of
the year.
• Sesamum is known by various names such as til,
sesame, gingelly, sim sim, benne etc.

5. • It has high oil content, good protein quality and methionine
to the extent of 50, 20-25 and 3.4 per cent respectively.
• Sesame oil is used as a cooking medium and forms the basis
of most scented oils.
• Sesame is used in religious festivals like Makarsankrant. It is
used in
• medicines and rich in protein, calcium, phosphorous and
vitamins and is a valuable food for cattle.
• Sesame plant offers several advantages such as shorter life
cycle, a potential for harvest index, tolerance to drought as
well as its ability to fit into a wider range of intercropping
systems and crop sequences.
Sesame has tremendous potential for export and accounts
for

6. • Genetical studies in sesamum are not as extensive as
sorghum, wheat, soybean, maize etc.
• The studies made so far in sesame were mainly
concerned with inheritance of quality characters
such as seed colour etc., having very little influence
on yield.
• The efficiency of selection depends upon the
identification of genetic variability by the
phenotypic expression of characters.
• The observed variability could be partitioned into
heritable (genetic) and non heritable (non-genetic)
components.
The genetic variability could be determined with the
help of certain genetic parameters such as genetic
coefficient of variation, heritability estimates and
genetic advance.

7. Objective
• 1. To study about the Genetic and inheritance pattern
study on sesame
• 2. To study the heterosis, inbreeding depression and
heritability for yield and yield component characters.
• 3.Identification of molecular characterization study on
(Sesamum indicum L.)

8. Technical Programme of Work
The experiment will be conducted at the Research
farm of Barrister Thakur Chhedilal College of
Agriculture and Research Station, Bilaspur
(Chhattisgarh), during kharif season 2019 with
the following technical programme:-

9. Experimental Details
 Year : 2019
 Design : Randomized block design
 Replications :
 Treatments :
 Gross plot size :
 Net plot size
 Spacing :- : Row to row : 45 cm.
 Plant to plant: 15 cm.
 Season : Kharif
 Crop : Sesame
 Seed rate : 5 kg /ha
 Sowing date : 25/08/2019
• Fertilizer dose (RDF) : 40:30:25 kg N: P2O5: K2O /ha
 Crop spacing : 45 cm × 15 cm
 Irrigation : Rainfed condition

10. Material and Methodology
• The present investigation was undertaken to study “study of Genetic
and inheritance pattern studies in sesame (Sesamum indicum L.)
Crosses”. The crosses for the present work were effected at BTC CARS
Bilaspur (C.G.).
• A set of 21 genotypes with parent and F2 Generation seed will be used
as experimental material for study. The experiment will be laid out in
CRBD in Kharif 2019.
• F1 Generation:-The 21 Genotype were crosses (Hybridization) Linked
to each other .
• The progenies of F1 were grown during Kharif 2019 recommended
package of practices for cultivation was adopted to rise the crop.
• F2 Generation:-
• Were F2 generation seed grow on Rabi sesaon 2019.
• The F2 population of Flowing crosses along with their parents were
grown separately and 100 plants from each population were randomly
selected and observation on these selected plants parents were
recorded on ten metric traits.

11. SN. Parental lines Seed color Maturity Day’s Silent feature
1. AKT-101 WHITE 90 -95 Sesame from drier
area
Less than 1%
oxalic acid
High oil content
2. C0-1 BLACK 90-95 Domestic and
medicinal uses
3. GT-10 BLACK 78-85 Good for Gujrat
plans zone
Domestic and
medicinal uses
Tolerant to
powdery mildew
4. JT-7 WHITE 78-82
5. JT-8 WHITE 78-82
6. N-32 WHITE 90-100 Moderately
resistance to leaf
spot and gallfly
stem borer

12. SN. Parental line Seed color Maturity Days Silent feature
7 NIRMALA BROWN White bold seeded good
export quality
8. THILRANI DARK /BROWN High yielding and high oil
content
9. THILO-THAMA BROWN 78 High oil content
Resistance to
microphomina stem rot
10. TKG-22 WHITE 76-81 Resistance to
Phytophathora Blight
Oil 53.3%
11. TKG-55 WHITE 76-81 Less then 2 % FFA Content
Same as TKG-22
12. TMV-3 BLACK 80 Tolerant to leaf minor
Rainfed and irrigated
areas of tamilnadu and
kerla

13. SN. Parental lines Seed color Silent feature
13 PHULE TIL WHITE Good for
maharastra
Bold seed 51%
oil.
14 THILAK DARK BROWN High yieding
varyties
Good weed
control
efficiency of
plant
15 THG-501 WHITE 78-80 High yieding
varyties
Good weed
control
efficiency of
plant
16 RT-346 BLACK

14. SN. Parental lines Seed color Silent feature
17 RT-46 WHITE 76-85 resistant to oozing
complex,
tolerant to
macrophomina stem
or root rot,
18 RT-54 WHITE /BROWN 73-83 Resistance to Leaf
blight & stem root rot
Tolerant to Antigastra
Oil-44.2%
19 RT-351 WHITE 90-95 Good for Rajasthan &
Gujrat up.
Low water
requrement
20 ROMA WHITE 85-90
21 PKDS-12 WHITE 82-85 Good for
MP.Maharastra
Resistance to Leaf
blight & stem root rot

15. Crop study
Sowing:- Dibbling of sesame seedling method was followed for sowing and 1
to 2 seeds per hill were dibbled on 15 september 2019
Cultural practices:- Recommended cultural/agronomic practices were
followed to raise a healthy crop.
Observations:-
Observations for the following characters were recorded on 21 genotype
randomly plants in F1 and F2 selected competitive plants, each on P1, P2
and 2 generations.
Days to 50 % flowering :-
Number of day’s required from sowing to flowering of
approximately 50 per cent plants in each genotype was recorded and the
average number of days to 50 per cent flowering was worked out
Number of branches per plant:-
The number ofbranches on main shoot were counted.
Number of capsules per plant:-
Capsules on main shoot and on all other branches were
counted and summed up.
Capsule length (cm):-
Capsule length was recorded in centimeters from tip of the

16. Contin…
. a ) Yield, yield attributes and quality parameter
1. Number of capsules per plant
2. Number of seed per capsule
3. Seed yield per plant (kg)
4. Number of branch per plant
5. 1000 seed waigth
6. Oil content in (%)
b) Some Genetic parameter analysis:
• Gene intraction
• Components of variance
• Coefficient of variability
• Heritability
• Genetic advance
• Correlation

17. • Statistical and biometrical analysis:
• Test for Goodness of fit
• Test of independence
• Detection of linkage
• Estimation of linkage
• Heterosis and inbreeding depresion
• Analysis of variance (ANOVA)
• Path coefficient analysis
d) Molecular characterization of genotypes:
For this following methodology will be followed:
• DNA Isolation
• DNA Quantification
• PCR amplification of genomic DNA with a sets of markers
• Gel documentation
• Scoring of markers and analysis of data