sachinverma1105@hau.ac.in

1. DUS CHARACTERIZATION
OF MUNGBEAN (VIGNA RADIATA)
Submitted to –
Dr. Ravika (Assistant Professor)
G&PB , CCS Haryana
Agricultural University, Hisar
Submitted by – Sachin verma
Ad. No.- (2020A70M)
M.Sc - Genetics and
Plant Breeding, COA, Hisar

2. GREEN GRAM CLASSIFICATION
• Kingdom : Plantae
• Order : Fabales
• Family : Fabaceae
• Genus :Vigna
• Species : radiata
 Chromosome number: (2n) = 2x =22
 It is the third most important self-pollinated
legume crop.

3. Why Characterization is needed ?
# Characterization of germplasm is the basic step in
forming groups of lines having similar
characteristics to get an idea of variability present
in the lines and their utilization in breeding
programmes .
# Essential requirement for registration of new
variety(s) under PPV & FR Act, 2001
Variety be examined for compliance with
Distinctiveness, Uniformity and Stability
criteria needed for registration.

4. What is DUS Test?
# Plant variety protection can only be granted in respect
of a new plant variety
after examination, for that UPOV Convention Acts
laid down criteria viz.
novelty (N), distinctness (D), uniformity (U) and
stability (S) ‘DUS’ or ‘NDUS’ in short.
# It Involves comparison of new (candidate) variety
against existing varieties for
recording a number of morphological/physiological
characters, by growing new
and existing varieties side-by-side

5. DISTINCTNESS
 Must be clearly distinguishable for at least one essential character from
the varieties of common knowledge in India and out side.
 Essential character is a heritable trait which is determined by one or more
genes
or other heritable determinants that contribute to the principal features,
performance or value of the plant variety.
UNIFORMITY
Uniform, when subjected to the variation that may be expected and allowed due
to the specific nature of the reproduction of the crop such as vegetative, self
and cross-pollinated.
STABILITY
Stable when all essential characters remain unchanged after repeated
propagation or specified cycle of propagation.

6. The seeds submitted shall have the following
standards:
a) Germination : 95% (Minimum)
b) Moisture content : 8-9% (Maximum)
c) Physical purity : 98% (Minimum)
d) Inert matter : 2-3% (Maximum)
The plant material shall not have been subjected
to any chemical and biophysical treatment.

7. Conduct of tests
1. The minimum duration of the DUS test shall normally be at
least two independent similar growing seasons.
2. The test shall normally be conducted at least at two test
locations. If any essential characteristics of the candidate
variety are not expressed for visual observation at these
locations, the variety shall be considered for further
examination at another appropriate test site or under special
test protocol.
3. The field test shall be carried out under conditions favoring
normal growth and expression of all test characteristics.
4. 4.Each test shall include about 500 plants across four
replications

8. Test plot Size
Number of rows 4 m
Row length 5 m
Row to row distance 45 cm
Plant to plant distance 15 cm
Expected plants/replication 140
Number of replications 3

9. Methods and observations
 For the assessment of Distinctiveness, and stability
observations shall be made on 30 plants or parts of 30 plants,
which shall be equally divided among 3 replications (10 plants
per replication).
 For the assessment of uniformity of characteristics on the plot
as a whole, which shall be done by single visual observation of
a group of plants or parts of plant. the number of off-types shall
not exceed
 For the assessment of all colour characteristics, the latest Royal
Horticultural Society (RHS)
colour chart shall be used.

10. OBSERVATIONS TO BE RECORDED
( Descriptors)
1. Hypocotyl: Anthocyanin coloration
2. Time of flowering
3. Plant growth habit
4. Plant habit
5. Stem colour
6. Stem pubescence
7. Leaflet lobes
8. Leaf shape
9. Leaf colour
10. Leaf vein colour
11. Petiole colour
10

11. Contd…
12. Leaf size 23. Seed shape
13. Flower colour 24. Seed size
14. Colour of premature pod
15. Pod Pubescence
16. Pod position
17. Plant height
18. Pod colour
19. Curvature of mature pod
20. Pod length
21. Seed colour
22. Seed luster
11

12. Grouping of varieties
The candidate varieties for DUS testing shall be divided
into groups to facilitate the assessment of
Distinctiveness .
The following characteristics are proposed to be used
for grouping mungbean varieties:
a) Hypocotyl: Anthocyanin colouration (Characteristic 1)
b) Time of flowering (Characteristic 2)
c) Seed: Lusture (Characteristic 22)
d) Seed: Size (Characteristic 24)

13. characteristics and their notation
1. Hypocotyl: Anthocyanin colouration

14. Pigmentation notation
Present - 9 Present - 9
Absent - 1 Absent - 1

15. S.No Characterstic States Note Example Stage of
Observation

16. Green stem - 1 Green with purple splash - 2 Purple - 3
S.No Characterstic States Note Example Stage of
Observation

17. S.No Characterstic States Note Example Stage of
Observation
Pubescence present - 9 Pubescence Absent - 1

18. S.No Characterstic States Note Example Stage of
Observation
Lobes Absent - 1 Lobes Present - 9

19. S.No Characterstic States Note Example Stage of
Observation

20. Green - 1
S.No Characterstic States Note Example Stage of
Observation
Drak Green - 2

21. S.No Characterstic States Not
e
Example Stage of
Observation

22. S.No Characterstic States Note Example Stage of
Observation
Yellow - 3 Light Yellow - 5

23. S.No Characterstic States Note Example Stage of
Observation

24. Green Pod – 1 Green pod - 2

25. S.No Characterstic States Note Example Stage of
Observation
Absent - 1 Present - 9

26. S.No Characterstic States Note Example Stage of
Observation

27. S.No Characterstic States Note Example Stage of
Observation
Brown - 1 Black - 2

28. S.No Characterstic States Note Example Stage of
Observation

29. Yellow - 1 Green - 2 Mottled – 3 Black - 4

30. S.No Characterstic States Note Example Stage of
Observation
Shiny – 1 Dull - 2

31. S.No Characterstic States Note Example Stage of
Observation
Oval shape seeds – 1 Drum shaped seeds - 3

32. S.No Characterstic States Note Example Stage of
Observation