The document summarizes Gram staining, a method developed by Hans Christian Gram in 1883 to differentiate between bacterial species. Gram staining uses crystal violet dye and iodine to stain bacteria, then decolorizes them with acetone or alcohol. Gram-positive bacteria retain the crystal violet dye after decolorization due to their thick peptidoglycan cell wall, appearing purple or blue. Gram-negative bacteria's thinner cell wall is unable to retain the dye after decolorization but can be counterstained pink with safranin. The test distinguishes between bacteria based on differences in cell wall chemistry and structure.
Acid fast staining is differential staining technique which differentiate bacteria into two group- acid fast bacteria and non acid bacteria. It used to identify acid-fast organisms such as members of the genus Mycobacterium .
Acid fast staining is differential staining technique which differentiate bacteria into two group- acid fast bacteria and non acid bacteria. It used to identify acid-fast organisms such as members of the genus Mycobacterium .
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Gram staining Principle, Procedure, Reagents required for Gram Staining and t...Zunaira Gillani
Gram staining Principle, Procedure, Reagents required for Gram Staining and their Functions, Peptidoglycan Structural difference in Gram positive and Gram Negative.
Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Culture medium or growth medium is a liquid or gel designed to support the growth of microorganisms. There are different types of media suitable for growing different types of cells. Here, we will discuss microbiological cultures used for growing microbes, such as bacteria ,fungi, yeast & algae.
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Gram staining Principle, Procedure, Reagents required for Gram Staining and t...Zunaira Gillani
Gram staining Principle, Procedure, Reagents required for Gram Staining and their Functions, Peptidoglycan Structural difference in Gram positive and Gram Negative.
Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Culture medium or growth medium is a liquid or gel designed to support the growth of microorganisms. There are different types of media suitable for growing different types of cells. Here, we will discuss microbiological cultures used for growing microbes, such as bacteria ,fungi, yeast & algae.
Berisi Informasi mengenai prosedur pewarnaan gram / gram staining dengan menggunakan kit dari Himedia
Informasi lebih lanjut, hubungi : delli@intralab.co.id | 0813-1136-5312
Gram stain is technique used to differntiate gram positive and gram negative bacteria.
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Bacteria are microscopic, single-celled organisms that thrive in diverse environments. These organisms can live in soil, the ocean and inside the human gut. Humans' relationship with bacteria is complex. Sometimes bacteria lend us a helping hand, such as by curdling milk into yogurt or helping with our digestion
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
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at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
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Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
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Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
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optics at visible wavelengths.
2. HANS CHRISTIAN GRAM
The Gram stain was devised
by the Danish physician,
Hans Christian Gram,
while working in Berlin in
1883. He later published
this procedure in 1884.
3. GRAM’S STAIN
Gram staining (or Gram's method) is a method of
differentiating bacterial species into two large groups
Gram Positive Gram Negative
Gram staining differentiates bacteria by the chemical and
physical properties of their cell walls.
4. GRAM POSITIVE BACTERIA
• Gram positive bacteria have a thick
cell wall of peptidoglycan.
• Peptidoglycan is a polymer
consisting of sugar amino acids
that form a mesh like outside the
plasma membrane of bacteria
forming cell wall.
• In Gram positive bacteria,
between the cell wall and cell
membrane, there is a "membrane
teichoic acid".
5. GRAM NEGATIVE BACTERIA
Gram negative bacteria have
an outer membrane of
phospholipids and bacterial
Lipopolysaccharides outside of
their thin peptidoglycan layer.
The space between the outer
membrane and the
peptidoglycan layer is called
the periplasmic space.
6.
7. PRINCIPLE OF GRAM’S STAINING
The structure of the organism ‘s cell wall
determines whether the organism is gram positive
or negative.
When stained with a primary stain and fixed by a
mordant, some bacteria are able to retain the
primary stain by resisting declorization while other
get decolorized by decolorizer.
Those bacteria which retain the primary stain are
called Gram positive.
Those bacteria which get decolorized and then get
counterstained are called Gram negative.
8. 1. Crystal violet - all bacteria take crystal violet- so all
appears violet.
2. Iodine – Crystal Violet-iodine(CV-I) complex is
formed.
3. Acetone- bacteria with high lipid content loose CV-I
complex(appear colourless) but bacteria with less
lipid content retains CV-I complex ( appear violet).
4. Safranine/ basic fuchsin – only colourless bacteria
takes – appear pink.
9.
10. PROCEDURE
1. Make a smear & dry thoroughly in cool air. Fix the dried
film by passing it briefly through a bunsen flame.
2. Flood the slide with crystal violate sol. for upto 1 min.
Wash off briefly with tap water & drain.
3.Flood the slide with gram’s iodine sol. & allow to act as
a mordant for about 1 min. Wash off with tap water &
drain.
11. 4.Decolourise the smear with acetone for 10-30 sec. taking
care not to overdecolourise & immediately wash off with
water.
5.Flood the slide with safranin sol. & counterstain for about
30 sec, wash off with tap water, drain & blot dry with filter
paper & examine under oil immersion objective.