Berisi Informasi mengenai prosedur pewarnaan gram / gram staining dengan menggunakan kit dari Himedia
Informasi lebih lanjut, hubungi : delli@intralab.co.id | 0813-1136-5312
This document provides instructions for Gram staining, a differential staining technique used to distinguish between bacteria based on their cell wall composition. It describes how Gram-positive bacteria retain the primary violet stain due to their thick peptidoglycan layer, appearing violet under the microscope, while Gram-negative bacteria's single peptidoglycan layer is decolorized by alcohol, causing them to appear pink when counterstained with safranin. The document outlines the Gram staining procedure and results, and examples of morphological appearances of common Gram-positive and Gram-negative bacteria.
Prosedur pewarnaan Gram melibatkan pewarnaan sampel dengan kristal ungu, yodium, dan safranin untuk membedakan bakteri Gram positif dan negatif. Sampel diwarnai kristal ungu selama 1 menit, lalu dicuci dan diwarnai yodium selama 1 menit. Proses deklorinasi menghilangkan warna biru dari bakteri Gram negatif sebelum counterstain dengan safranin selama 1 menit. Hasil pemeriksaan dengan mikroskop menunjuk
This document provides information about staining microorganisms. It begins by defining what a stain is and explaining that different stains can differentiate between organism types or parts. It then discusses the history of stains and the structures and mechanisms of various dyes used in staining. The remainder of the document outlines different staining techniques such as simple stains, differential stains like Gram staining, and special stains for specific structures. It provides details on reagents, principles, and methods for common staining procedures.
1. The document discusses various culture media used for cultivating microorganisms from clinical specimens. It describes the composition and purpose of different types of media including basal media, enriched media, selective media, and transport media.
2. Specific media are described for cultivating gram positive cocci like Staphylococci and Streptococci. Mannitol salt agar, tellurite glycine agar, and DNase test agar are discussed for isolating and identifying Staphylococcus. Todd Hewitt broth and crystal violet blood agar are mentioned for Streptococci.
3. Modified Thayer Martin medium and modified New York City medium are highlighted as selective media used for isolating Neisseria
This document discusses various staining techniques used to visualize cells and internal structures. It describes the basic components and principles of dyes and stains, including chromophores, auxochromes and benzene rings. It outlines different types of stains categorized by molecular structure and electric charge. Basic techniques like simple staining, gram staining and acid-fast staining are explained in detail. The document compares properties of gram-positive and gram-negative cell walls. It provides examples of structures that are acid-fast and the importance of Ziehl-Neelsen staining for detecting Mycobacterium tuberculosis bacilli.
This document outlines the objectives and content of lectures on mycology. It discusses the general characteristics and morphology of fungi, their classification, and laboratory diagnosis of fungal diseases. Key points covered include the different types of fungi (yeast, molds, dimorphic), their structures (cell wall, capsule, hyphae), modes of reproduction (sexual producing spores, asexual producing conidia or blastospores), taxonomic classification, specimen collection and culture methods (media, stains) for diagnosis, and tests like the germ tube test. The goals are to define mycology terms, describe fungal morphology and medical importance, and review methods for laboratory diagnosis of fungal infections.
This document provides instructions for Gram staining, a differential staining technique used to distinguish between bacteria based on their cell wall composition. It describes how Gram-positive bacteria retain the primary violet stain due to their thick peptidoglycan layer, appearing violet under the microscope, while Gram-negative bacteria's single peptidoglycan layer is decolorized by alcohol, causing them to appear pink when counterstained with safranin. The document outlines the Gram staining procedure and results, and examples of morphological appearances of common Gram-positive and Gram-negative bacteria.
Prosedur pewarnaan Gram melibatkan pewarnaan sampel dengan kristal ungu, yodium, dan safranin untuk membedakan bakteri Gram positif dan negatif. Sampel diwarnai kristal ungu selama 1 menit, lalu dicuci dan diwarnai yodium selama 1 menit. Proses deklorinasi menghilangkan warna biru dari bakteri Gram negatif sebelum counterstain dengan safranin selama 1 menit. Hasil pemeriksaan dengan mikroskop menunjuk
This document provides information about staining microorganisms. It begins by defining what a stain is and explaining that different stains can differentiate between organism types or parts. It then discusses the history of stains and the structures and mechanisms of various dyes used in staining. The remainder of the document outlines different staining techniques such as simple stains, differential stains like Gram staining, and special stains for specific structures. It provides details on reagents, principles, and methods for common staining procedures.
1. The document discusses various culture media used for cultivating microorganisms from clinical specimens. It describes the composition and purpose of different types of media including basal media, enriched media, selective media, and transport media.
2. Specific media are described for cultivating gram positive cocci like Staphylococci and Streptococci. Mannitol salt agar, tellurite glycine agar, and DNase test agar are discussed for isolating and identifying Staphylococcus. Todd Hewitt broth and crystal violet blood agar are mentioned for Streptococci.
3. Modified Thayer Martin medium and modified New York City medium are highlighted as selective media used for isolating Neisseria
This document discusses various staining techniques used to visualize cells and internal structures. It describes the basic components and principles of dyes and stains, including chromophores, auxochromes and benzene rings. It outlines different types of stains categorized by molecular structure and electric charge. Basic techniques like simple staining, gram staining and acid-fast staining are explained in detail. The document compares properties of gram-positive and gram-negative cell walls. It provides examples of structures that are acid-fast and the importance of Ziehl-Neelsen staining for detecting Mycobacterium tuberculosis bacilli.
This document outlines the objectives and content of lectures on mycology. It discusses the general characteristics and morphology of fungi, their classification, and laboratory diagnosis of fungal diseases. Key points covered include the different types of fungi (yeast, molds, dimorphic), their structures (cell wall, capsule, hyphae), modes of reproduction (sexual producing spores, asexual producing conidia or blastospores), taxonomic classification, specimen collection and culture methods (media, stains) for diagnosis, and tests like the germ tube test. The goals are to define mycology terms, describe fungal morphology and medical importance, and review methods for laboratory diagnosis of fungal infections.
1. The document discusses different microscopic techniques used to observe microorganisms, including growing and isolating bacteria, observing them under brightfield and phase contrast microscopes, and staining them using techniques like Gram staining and acid-fast staining.
2. It explains the basic parts and functions of a light microscope like the objective lenses, condenser, diaphragm, and describes different types of microscopes and maximum magnifications.
3. Special staining techniques are described that allow visualization of specific structures like capsules, flagella, endospores using stains like India ink, malachite green, and the five basic groups of microorganisms are identified.
This document provides information about interpreting Gram stains and describes the morphology of various bacteria under the Gram stain. It begins by explaining the Gram stain procedure and how to judge the quality of a Gram stain. It then describes the appearance of many Gram-positive and Gram-negative bacteria including cocci, rods, fungi and some human cells. Key details about cell arrangement, shape, size and other distinguishing features are provided for common genera like Staphylococcus, Streptococcus, Bacillus, Pseudomonas and Neisseria.
The document discusses various staining techniques used in microbiology, including Gram staining, acid-fast staining, and simple staining techniques. Gram staining differentiates bacteria into gram-positive and gram-negative groups based on differences in their cell wall structure and how they retain or release crystal violet dye. Acid-fast staining uses a carbolfuchsin primary stain to identify acid-fast bacteria that resist decolorization by acid-alcohol, such as Mycobacterium tuberculosis. Simple stains like Loeffler's methylene blue and diluted carbol fuchsin are also discussed, which provide contrast but do not differentiate bacterial types.
The Gram stain technique classifies bacteria as Gram positive or Gram negative based on differences in cell wall structure. Gram positive bacteria have a thick peptidoglycan layer and retain crystal violet dye after ethanol washing. Gram negative bacteria have a thin peptidoglycan layer between inner and outer membranes and lose the crystal violet dye after ethanol washing, taking up the counterstain safranin and appearing pink. The Gram stain procedure involves applying and washing crystal violet, adding an iodine mordant, washing with ethanol, and counterstaining with safranin.
The document summarizes Gram staining, a method developed by Hans Christian Gram in 1883 to differentiate between bacterial species. Gram staining uses crystal violet dye and iodine to stain bacteria, then decolorizes them with acetone or alcohol. Gram-positive bacteria retain the crystal violet dye after decolorization due to their thick peptidoglycan cell wall, appearing purple or blue. Gram-negative bacteria's thinner cell wall is unable to retain the dye after decolorization but can be counterstained pink with safranin. The test distinguishes between bacteria based on differences in cell wall chemistry and structure.
1. Gram staining is a differential staining technique developed by Hans Christian Gram in 1884 that is used to classify bacteria into two groups: Gram-positive and Gram-negative.
2. The key steps of Gram staining involve staining with crystal violet dye, treating with iodine, decolorizing with alcohol or acetone, and counterstaining with safranin.
3. Gram-positive bacteria retain the crystal violet dye after decolorization due to their thick peptidoglycan cell wall, while Gram-negative bacteria lose the dye due to their thinner cell wall. This allows bacteria to be classified based on their staining.
Gram staining is a method used to differentiate between two major types of bacterial cell walls - Gram-positive and Gram-negative. Gram-positive bacteria have cell walls containing large amounts of peptidoglycan and no lipopolysaccharide, while Gram-negative bacteria have cell walls containing small amounts of peptidoglycan and lipopolysaccharide. The exact mechanism of Gram staining is not fully understood. Gram-negative bacteria are more resistant to antibiotics and lysozyme than Gram-positive bacteria due to differences in cell wall structure.
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Este documento describe las características del género Staphylococcus, incluyendo su morfología, tinción de Gram, principales especies (S. aureus, S. epidermidis, S. saprophyticus) y cuadros clínicos producidos por acción directa e indirecta. También cubre temas como diagnóstico bacteriológico y tratamiento de infecciones por Staphylococcus.
This document provides information about Gram staining, a technique used to classify bacteria. It describes how Gram-positive bacteria retain the crystal violet stain due to their thick peptidoglycan layer, while Gram-negative bacteria do not retain the stain due to their thin peptidoglycan layer and outer membrane. The document outlines the Gram staining procedure and reagents used, and provides examples of morphological characteristics of different bacteria under Gram staining.
This document discusses various bacterial staining techniques used to visualize microorganisms under a microscope. It describes simple staining which uses a single dye, differential staining which allows differentiation using more than one dye, and special staining techniques to highlight specific structures. Gram staining is explained in detail as the most common differential staining method used to classify bacteria as gram-positive or gram-negative. Acid-fast staining and capsule, spore, and flagella staining are also summarized as important special staining methods.
Microbiological Environmental Monitoring in Pharmaceutical Facilitydelli_intralab
Merupakan jurnal tentang microbiological environment monitoring in pharma facility
Untuk informasi lebih lanjut atau diskusi mengenai environment monitoring, silahkan hubungi delli.intralab@gmail.com
Merupakan penggalan USP 36 chapter 1116 mengenai Microbiological Control And Monitoring Of Aseptic Processing Environments
Untuk mendapat softcopy atau informasi lebih lanjut silahkan hubungi delli.intralab@gmail.com
Dokumen tersebut memberikan informasi mengenai arsenik yang dapat ditemukan dalam air tanah dan sumur, serta potensi bahaya kesehatan dari paparan arsenik jangka panjang. Dijelaskan pula cara mudah mengetahui kadar arsenik menggunakan alat tes arsenik sederhana.
1. The document discusses different microscopic techniques used to observe microorganisms, including growing and isolating bacteria, observing them under brightfield and phase contrast microscopes, and staining them using techniques like Gram staining and acid-fast staining.
2. It explains the basic parts and functions of a light microscope like the objective lenses, condenser, diaphragm, and describes different types of microscopes and maximum magnifications.
3. Special staining techniques are described that allow visualization of specific structures like capsules, flagella, endospores using stains like India ink, malachite green, and the five basic groups of microorganisms are identified.
This document provides information about interpreting Gram stains and describes the morphology of various bacteria under the Gram stain. It begins by explaining the Gram stain procedure and how to judge the quality of a Gram stain. It then describes the appearance of many Gram-positive and Gram-negative bacteria including cocci, rods, fungi and some human cells. Key details about cell arrangement, shape, size and other distinguishing features are provided for common genera like Staphylococcus, Streptococcus, Bacillus, Pseudomonas and Neisseria.
The document discusses various staining techniques used in microbiology, including Gram staining, acid-fast staining, and simple staining techniques. Gram staining differentiates bacteria into gram-positive and gram-negative groups based on differences in their cell wall structure and how they retain or release crystal violet dye. Acid-fast staining uses a carbolfuchsin primary stain to identify acid-fast bacteria that resist decolorization by acid-alcohol, such as Mycobacterium tuberculosis. Simple stains like Loeffler's methylene blue and diluted carbol fuchsin are also discussed, which provide contrast but do not differentiate bacterial types.
The Gram stain technique classifies bacteria as Gram positive or Gram negative based on differences in cell wall structure. Gram positive bacteria have a thick peptidoglycan layer and retain crystal violet dye after ethanol washing. Gram negative bacteria have a thin peptidoglycan layer between inner and outer membranes and lose the crystal violet dye after ethanol washing, taking up the counterstain safranin and appearing pink. The Gram stain procedure involves applying and washing crystal violet, adding an iodine mordant, washing with ethanol, and counterstaining with safranin.
The document summarizes Gram staining, a method developed by Hans Christian Gram in 1883 to differentiate between bacterial species. Gram staining uses crystal violet dye and iodine to stain bacteria, then decolorizes them with acetone or alcohol. Gram-positive bacteria retain the crystal violet dye after decolorization due to their thick peptidoglycan cell wall, appearing purple or blue. Gram-negative bacteria's thinner cell wall is unable to retain the dye after decolorization but can be counterstained pink with safranin. The test distinguishes between bacteria based on differences in cell wall chemistry and structure.
1. Gram staining is a differential staining technique developed by Hans Christian Gram in 1884 that is used to classify bacteria into two groups: Gram-positive and Gram-negative.
2. The key steps of Gram staining involve staining with crystal violet dye, treating with iodine, decolorizing with alcohol or acetone, and counterstaining with safranin.
3. Gram-positive bacteria retain the crystal violet dye after decolorization due to their thick peptidoglycan cell wall, while Gram-negative bacteria lose the dye due to their thinner cell wall. This allows bacteria to be classified based on their staining.
Gram staining is a method used to differentiate between two major types of bacterial cell walls - Gram-positive and Gram-negative. Gram-positive bacteria have cell walls containing large amounts of peptidoglycan and no lipopolysaccharide, while Gram-negative bacteria have cell walls containing small amounts of peptidoglycan and lipopolysaccharide. The exact mechanism of Gram staining is not fully understood. Gram-negative bacteria are more resistant to antibiotics and lysozyme than Gram-positive bacteria due to differences in cell wall structure.
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Este documento describe las características del género Staphylococcus, incluyendo su morfología, tinción de Gram, principales especies (S. aureus, S. epidermidis, S. saprophyticus) y cuadros clínicos producidos por acción directa e indirecta. También cubre temas como diagnóstico bacteriológico y tratamiento de infecciones por Staphylococcus.
This document provides information about Gram staining, a technique used to classify bacteria. It describes how Gram-positive bacteria retain the crystal violet stain due to their thick peptidoglycan layer, while Gram-negative bacteria do not retain the stain due to their thin peptidoglycan layer and outer membrane. The document outlines the Gram staining procedure and reagents used, and provides examples of morphological characteristics of different bacteria under Gram staining.
This document discusses various bacterial staining techniques used to visualize microorganisms under a microscope. It describes simple staining which uses a single dye, differential staining which allows differentiation using more than one dye, and special staining techniques to highlight specific structures. Gram staining is explained in detail as the most common differential staining method used to classify bacteria as gram-positive or gram-negative. Acid-fast staining and capsule, spore, and flagella staining are also summarized as important special staining methods.
Microbiological Environmental Monitoring in Pharmaceutical Facilitydelli_intralab
Merupakan jurnal tentang microbiological environment monitoring in pharma facility
Untuk informasi lebih lanjut atau diskusi mengenai environment monitoring, silahkan hubungi delli.intralab@gmail.com
Merupakan penggalan USP 36 chapter 1116 mengenai Microbiological Control And Monitoring Of Aseptic Processing Environments
Untuk mendapat softcopy atau informasi lebih lanjut silahkan hubungi delli.intralab@gmail.com
Dokumen tersebut memberikan informasi mengenai arsenik yang dapat ditemukan dalam air tanah dan sumur, serta potensi bahaya kesehatan dari paparan arsenik jangka panjang. Dijelaskan pula cara mudah mengetahui kadar arsenik menggunakan alat tes arsenik sederhana.
Modul Ajar Bahasa Inggris Kelas 10 Fase E Kurikulum MerdekaFathan Emran
Modul Ajar Bahasa Inggris Kelas 10 SMA/MA Fase E Kurikulum Merdeka - abdiera.com. Modul Ajar Bahasa Inggris Kelas 10 SMA/MA Fase E Kurikulum Merdeka. Modul Ajar Bahasa Inggris Kelas 10 SMA/MA Fase E Kurikulum Merdeka.
Ppt landasan pendidikan Pai 9 _20240604_231000_0000.pdffadlurrahman260903
Ppt landasan pendidikan tentang pendidikan seumur hidup.
Prodi pendidikan agama Islam
Fakultas tarbiyah dan ilmu keguruan
Universitas Islam negeri syekh Ali Hasan Ahmad addary Padangsidimpuan
Pendidikan sepanjang hayat atau pendidikan seumur hidup adalah sebuah system konsepkonsep pendidikan yang menerangkan keseluruhan peristiwa-peristiwa kegiatan belajarmengajar yang berlangsung dalam keseluruhan kehidupan manusia. Pendidikan sepanjang
hayat memandang jauh ke depan, berusaha untuk menghasilkan manusia dan masyarakat yang
baru, merupakan suatu proyek masyarakat yang sangat besar. Pendidikan sepanjang hayat
merupakan asas pendidikan yang cocok bagi orang-orang yang hidup dalam dunia
transformasi dan informasi, yaitu masyarakat modern. Manusia harus lebih bisa menyesuaikan
dirinya secara terus menerus dengan situasi yang baru.
12. Prosedur Pewarnaan Gram
1
Siapkan hapusan tipis sampel pada objek
glas yang kering dan bersih.
2
Keringkan dengan udara, kemudian lakukan
fiksasi dengan perlahan.
13. Prosedur Pewarnaan Gram
1
Siapkan hapusan tipis sampel pada objek
glas yang kering dan bersih.
2
Keringkan dengan udara, kemudian lakukan
fiksasi dengan perlahan.
3
Tuang dengan Gram’s Crystal Violet ( S012
). Biarkan selama 1 menit.
14. Prosedur Pewarnaan Gram
1
Siapkan hapusan tipis sampel pada objek
glas yang kering dan bersih.
2
Keringkan dengan udara, kemudian lakukan
fiksasi dengan perlahan.
3
Tuang dengan Gram’s Crystal Violet ( S012
). Biarkan selama 1 menit.
4
Cuci dengan air bersih ( Aquadest ).
16. Prosedur Pewarnaan Gram
5
Tuang hapusan dengan Grams Iodine (
S013). Biarkan selama 1 menit.
6
Lakukan proses decoulorisasi dengan Gram
Decoulorizer ( S032 ) sampai warna biru
tidak lagi mengalir dari hapusan.
17. Prosedur Pewarnaan Gram
5
Tuang hapusan dengan Grams Iodine (
S013). Biarkan selama 1 menit.
6
Lakukan proses decoulorisasi dengan Gram
Decoulorizer ( S032 ) sampai warna biru
tidak lagi mengalir dari hapusan.
7
Cuci dengan air bersih ( Aquadest )
19. Prosedur Pewarnaan Gram
8
Counter stain dengan 0.25 % w/v Safranin (
S027 ). Biarkan selama 1 menit.
9
Cuci dengan air ( Aquabidest ).
20. Prosedur Pewarnaan Gram
8
Counter stain dengan 0.25 % w/v Safranin (
S027 ). Biarkan selama 1 menit.
9
Cuci dengan air ( Aquabidest ).
10
Keringkan object glass pada udara terbuka
atau gunakan tissu pengering khusus.
21. Prosedur Pewarnaan Gram
8
Counter stain dengan 0.25 % w/v Safranin (
S027 ). Biarkan selama 1 menit.
9
Cuci dengan air ( Aquabidest ).
10
Keringkan object glass pada udara terbuka
atau gunakan tissu pengering khusus.
11
Periksa dengan Menggunakan mikroskop.