Culture medium is a substance used to grow microorganisms outside the body. It provides nutrients like carbon, nitrogen, minerals and growth factors. Culture media can be solid, semi-solid or liquid. They are classified as simple, complex, synthetic or special based on ingredients. Special media include enriched media which adds substances like blood or serum, and selective media which uses antibiotics to inhibit certain bacteria. Differential and transport media are also used to identify bacteria or safely transport clinical samples for identification.
Details about all type of culture media for growing the bacteria,
the basic constituents of culture media
types of media
simple media
special media
complex media
transport media
sugar media
anaerobic media
Giving basic concepts regarding culture media and its classification on the basis of different properties like physical state, chemical composition and utility purposes.
Details about all type of culture media for growing the bacteria,
the basic constituents of culture media
types of media
simple media
special media
complex media
transport media
sugar media
anaerobic media
Giving basic concepts regarding culture media and its classification on the basis of different properties like physical state, chemical composition and utility purposes.
Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Acid fast staining is differential staining technique which differentiate bacteria into two group- acid fast bacteria and non acid bacteria. It used to identify acid-fast organisms such as members of the genus Mycobacterium .
A simple lecture for the description of the various culture media used for isolation of different bacteria in a pure form for further identification procedures.
Gram staining Principle, Procedure, Reagents required for Gram Staining and t...Zunaira Gillani
Gram staining Principle, Procedure, Reagents required for Gram Staining and their Functions, Peptidoglycan Structural difference in Gram positive and Gram Negative.
Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Acid fast staining is differential staining technique which differentiate bacteria into two group- acid fast bacteria and non acid bacteria. It used to identify acid-fast organisms such as members of the genus Mycobacterium .
A simple lecture for the description of the various culture media used for isolation of different bacteria in a pure form for further identification procedures.
Gram staining Principle, Procedure, Reagents required for Gram Staining and t...Zunaira Gillani
Gram staining Principle, Procedure, Reagents required for Gram Staining and their Functions, Peptidoglycan Structural difference in Gram positive and Gram Negative.
Culture medium or growth medium is a liquid or gel designed to support the growth of microorganisms. There are different types of media suitable for growing different types of cells. Here, we will discuss microbiological cultures used for growing microbes, such as bacteria ,fungi, yeast & algae.
Nutrient media – A source of amino acids and nitrogen (e.g., beef, yeast extract). This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown
These slides explain how media preparation in microbiology lab
for bacterial culture, history of culture media, types of culture media based on concentration and ingredients and practical section.
R3 Stem Cells and Kidney Repair A New Horizon in Nephrology.pptxR3 Stem Cell
R3 Stem Cells and Kidney Repair: A New Horizon in Nephrology" explores groundbreaking advancements in the use of R3 stem cells for kidney disease treatment. This insightful piece delves into the potential of these cells to regenerate damaged kidney tissue, offering new hope for patients and reshaping the future of nephrology.
Health Education on prevention of hypertensionRadhika kulvi
Hypertension is a chronic condition of concern due to its role in the causation of coronary heart diseases. Hypertension is a worldwide epidemic and important risk factor for coronary artery disease, stroke and renal diseases. Blood pressure is the force exerted by the blood against the walls of the blood vessels and is sufficient to maintain tissue perfusion during activity and rest. Hypertension is sustained elevation of BP. In adults, HTN exists when systolic blood pressure is equal to or greater than 140mmHg or diastolic BP is equal to or greater than 90mmHg. The
Medical Technology Tackles New Health Care Demand - Research Report - March 2...pchutichetpong
M Capital Group (“MCG”) predicts that with, against, despite, and even without the global pandemic, the medical technology (MedTech) industry shows signs of continuous healthy growth, driven by smaller, faster, and cheaper devices, growing demand for home-based applications, technological innovation, strategic acquisitions, investments, and SPAC listings. MCG predicts that this should reflects itself in annual growth of over 6%, well beyond 2028.
According to Chris Mouchabhani, Managing Partner at M Capital Group, “Despite all economic scenarios that one may consider, beyond overall economic shocks, medical technology should remain one of the most promising and robust sectors over the short to medium term and well beyond 2028.”
There is a movement towards home-based care for the elderly, next generation scanning and MRI devices, wearable technology, artificial intelligence incorporation, and online connectivity. Experts also see a focus on predictive, preventive, personalized, participatory, and precision medicine, with rising levels of integration of home care and technological innovation.
The average cost of treatment has been rising across the board, creating additional financial burdens to governments, healthcare providers and insurance companies. According to MCG, cost-per-inpatient-stay in the United States alone rose on average annually by over 13% between 2014 to 2021, leading MedTech to focus research efforts on optimized medical equipment at lower price points, whilst emphasizing portability and ease of use. Namely, 46% of the 1,008 medical technology companies in the 2021 MedTech Innovator (“MTI”) database are focusing on prevention, wellness, detection, or diagnosis, signaling a clear push for preventive care to also tackle costs.
In addition, there has also been a lasting impact on consumer and medical demand for home care, supported by the pandemic. Lockdowns, closure of care facilities, and healthcare systems subjected to capacity pressure, accelerated demand away from traditional inpatient care. Now, outpatient care solutions are driving industry production, with nearly 70% of recent diagnostics start-up companies producing products in areas such as ambulatory clinics, at-home care, and self-administered diagnostics.
CHAPTER 1 SEMESTER V - ROLE OF PEADIATRIC NURSE.pdfSachin Sharma
Pediatric nurses play a vital role in the health and well-being of children. Their responsibilities are wide-ranging, and their objectives can be categorized into several key areas:
1. Direct Patient Care:
Objective: Provide comprehensive and compassionate care to infants, children, and adolescents in various healthcare settings (hospitals, clinics, etc.).
This includes tasks like:
Monitoring vital signs and physical condition.
Administering medications and treatments.
Performing procedures as directed by doctors.
Assisting with daily living activities (bathing, feeding).
Providing emotional support and pain management.
2. Health Promotion and Education:
Objective: Promote healthy behaviors and educate children, families, and communities about preventive healthcare.
This includes tasks like:
Administering vaccinations.
Providing education on nutrition, hygiene, and development.
Offering breastfeeding and childbirth support.
Counseling families on safety and injury prevention.
3. Collaboration and Advocacy:
Objective: Collaborate effectively with doctors, social workers, therapists, and other healthcare professionals to ensure coordinated care for children.
Objective: Advocate for the rights and best interests of their patients, especially when children cannot speak for themselves.
This includes tasks like:
Communicating effectively with healthcare teams.
Identifying and addressing potential risks to child welfare.
Educating families about their child's condition and treatment options.
4. Professional Development and Research:
Objective: Stay up-to-date on the latest advancements in pediatric healthcare through continuing education and research.
Objective: Contribute to improving the quality of care for children by participating in research initiatives.
This includes tasks like:
Attending workshops and conferences on pediatric nursing.
Participating in clinical trials related to child health.
Implementing evidence-based practices into their daily routines.
By fulfilling these objectives, pediatric nurses play a crucial role in ensuring the optimal health and well-being of children throughout all stages of their development.
How many patients does case series should have In comparison to case reports.pdfpubrica101
Pubrica’s team of researchers and writers create scientific and medical research articles, which may be important resources for authors and practitioners. Pubrica medical writers assist you in creating and revising the introduction by alerting the reader to gaps in the chosen study subject. Our professionals understand the order in which the hypothesis topic is followed by the broad subject, the issue, and the backdrop.
https://pubrica.com/academy/case-study-or-series/how-many-patients-does-case-series-should-have-in-comparison-to-case-reports/
Empowering ACOs: Leveraging Quality Management Tools for MIPS and BeyondHealth Catalyst
Join us as we delve into the crucial realm of quality reporting for MSSP (Medicare Shared Savings Program) Accountable Care Organizations (ACOs).
In this session, we will explore how a robust quality management solution can empower your organization to meet regulatory requirements and improve processes for MIPS reporting and internal quality programs. Learn how our MeasureAble application enables compliance and fosters continuous improvement.
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
Growing Prevalence of Lifestyle Diseases
The rising incidence of lifestyle diseases such as diabetes, cardiovascular diseases, and cancer is a major trend driving the clinical trials market in India. These conditions necessitate the development and testing of new treatment methods, creating a robust demand for clinical trials. The increasing burden of these diseases highlights the need for innovative therapies and underscores the importance of India as a key player in global clinical research.
2. What is culture medium ?
The food material or substances required for
growing microorganisms in vitro (outside the
body) is called culture medium.
3. Uses of culture medium
• It is important to grow microorganisms
outside the body for the following purposes:
1. to identify the cause of infection from the
clinical sample, so that proper treatment can
be given.
2. to study the characteristics or properties of
microorganisms.
3. to prepare biological products like vaccines,
toxoides, antigens ... etc.
4. Composition of culture media
• Water
• Energy source
• Carbon source
• Nitrogen source
• Mineral salts
• Special growth factors
5. Types of culture media
I . Classification based on physical state
a) solid medium
b) semi solid medium
c) liquid medium
6. II. Classification based on the
ingredients
a) simple medium
b) complex medium
c) synthetic or defined medium
d) Special media
7. Classification based on physical state
Solid medium
agar is the most commonly used solidifying agent.
What is agar
Golden -yellow granular powder
Prepared from seaweeds.
Not affected by the growth of the bacteria
Melts at 98°C & sets at 42°C
8. Semi-solid media
Such media are soft and are useful in
demonstrating bacterial motility and
separating motile from non motile strains
9. Liquid media
• Liquid media are sometimes referred as"
broth ".
• Bacteria grow uniformly producing general
turbidity eg. Nutrient broth
10. Classification based on the
ingredients
• Simple media
- eg: Nutrient broth, Nutrient agar
- NB consists of peptone, meat extract, NaCl,
- NB+ 2% agar= Nutrient agar
11. Complex media
Media that contain some ingredients of
unknown chemical composition are complex
media such as blood agar.
Complex media contain undefined components
like peptones, meat extract and yeast extract.
12. Synthetic or defined media
specially prepared media from pure chemical
substances for research purpose and
composition of every component is well
known
• eg: peptone water -
• 1% peptone + 0.5% NaCl in water.
14. Enriched media
• Substances like blood, serum, egg are added
to the simple medium.
• Used to grow bacteria that are exacting in
their nutritional needs.
• eg: Blood agar, Chocolate agar
15. Blood agar
• BAP contains mammalian blood(usually
sheep or horse) typically at a concentration of
5-10%,
• used to isolate fastidious organisms and
detect hemolysis.
16. Chocolate agar
• contain red blood cells that have been lysed
by slowly heating to 80°C .and it used for
growing
• fastidious bacteria, such as Haemophilus
influenzae
17. Selective media
The inhibitory substance is added to a solid
media to inhibit commensal or contaminating
bacteria such as :
• Antibiotics
• Dyes
• Chemicals
• Alteration of pH
18. Examples
Thayer Martin medium
• selective for Neisseria gonorrhoeae
• It usually contains the following combination of
antibiotics:
• Vancomycin:
• Colistin,:
• Nystatin,:
• Trimethoprim:
19. Eosin methylene blue
selective for gram negative bacteria
• The dye methylene blue in the medium inhibit
the growth of gram positive bacteria.
•
20. Campylobacter agar
Is used for isolation of Campylobacter jejuni
from fecal or rectal swab.
• Contain Bacteriological charcoal,
Cefoperazone and Amphotericin B.
21. Lowenstein -Jenson medium
LJ medium is solid medium used for
Mycobacterium tuberculosis.
• contain penicillin, nalidixic acid and
malachite green to inhibit growth of gram
positive and gram negative bacteria, in order
to limit growth to Mycobacteria species only.
22. Differential media
• Differential media are designed in such a way that
different bacteria can be recognized on the basis of their
colony color.
• Dyes and metabolic substrates are incorporated so that
those bacteria that utilize them appear as differently
colored colonies.
• Examples:
• MacConkey agar
• • CLED agar
• • TCBS agar
• • XLD agar
23. • Examples
• MacConkey medium
Distinguish between lactose fermenters & non
lactose fermenters.
Lactose fermenters - Pink colonies
Non lactose fermenters - colorless colonies
24. • Examples
Cysteine Lactose Electrolyte Deficient
• Agar (CLED)
• For cultivation of pathogen from urine
specimen, inhibit swarming of proteus sp.
25. Transport media
• Media used for transporting the samples.
• Delicate organisms may not survive the time
taken for transporting the specimen without a
transport media.
• Eg:
• Stuart's medium
• Buffered glycerol saline
26. Anaerobic media
These media are used to grow anaerobic
organisms.
• Eg:
• Robertson's cooked meat medium.
• Thioglycolate broth medium.