Site directed mutgenesis, OLIGONUCLEOTIDE DIRECTED MUTAGENESIS Vipin Shukla
INTRODUCTION, HISTORY, MUTATION, DIRECTED MUTAGENESIS,BASIC MECHANISM OF SITE DIRECTED MUTAGENESIS,METHOD FOR SITE DIRECTED MUTATIONS,THE SINGLE PRIMER METHOD, CASETTEE MUTAGENESIS, PCR-SITED DIRECTED MUTAGENESIS, APPLICATION OF SITE DIRECTED MUTAGENESIS.
Site directed mutgenesis, OLIGONUCLEOTIDE DIRECTED MUTAGENESIS Vipin Shukla
INTRODUCTION, HISTORY, MUTATION, DIRECTED MUTAGENESIS,BASIC MECHANISM OF SITE DIRECTED MUTAGENESIS,METHOD FOR SITE DIRECTED MUTATIONS,THE SINGLE PRIMER METHOD, CASETTEE MUTAGENESIS, PCR-SITED DIRECTED MUTAGENESIS, APPLICATION OF SITE DIRECTED MUTAGENESIS.
lac operon is a negatively controlled inducible operon.E.coli can use lactose as a source of carbon.
The enzymes required for the use of lactose as a source of carbon are synthesised only when the lactose is available as carbon source.
The lac operon is an example of nagatively controlled inducible operon.
Structure
The lac operon consists of 5 structural units.
Promoter
Operator
Structural genes
CAP binding sites
Regulatory gene
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
A hyperlinked and animated PowerPoint presentation on DNA transcription, its stages, units, etc.
Hope you will like it.
Please do share with your friends
lac operon is a negatively controlled inducible operon.E.coli can use lactose as a source of carbon.
The enzymes required for the use of lactose as a source of carbon are synthesised only when the lactose is available as carbon source.
The lac operon is an example of nagatively controlled inducible operon.
Structure
The lac operon consists of 5 structural units.
Promoter
Operator
Structural genes
CAP binding sites
Regulatory gene
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
A hyperlinked and animated PowerPoint presentation on DNA transcription, its stages, units, etc.
Hope you will like it.
Please do share with your friends
In my report on the cell cycle and its checkpoints, I delve into the phases of cell division and the regulatory mechanisms that ensure accurate replication. By examining these checkpoints, I uncover the essential processes that safeguard genomic integrity and maintain organismal function.
Control of gene expression ppt
definition of gene expression
inducible gene expression
repressible gene expression
control of gene expression in eukaryotics .all the in information about this topic is include .
presented by HAFIZ M WASEEM
university of education LAHORE Pakistan
i am from mailsi vehari and studied in lahore
bsc in science college multan
msc from lahore
Gene regulation is the process used to control the timing, location and amount in which genes are expressed. The process can be complicated and is carried out by a variety of mechanisms, including through regulatory proteins and chemical modification of DNA.
Regulation of gene expression.
It can be helpful for the students of Biotechnology, Genetics, Molecular Biology, Microbiology and othe Biology related courses.
If you've got any queries, you can directly mail me to pratimasingdan@gmail.com.
I hope this will help you a lot.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
2. Definition
Gene regulation is the process of turning genes on
and off.
During early development, cells begin to take on
specific functions.
Gene regulation ensures that the appropriate
genes are expressed at the proper times
It is the process that control the rate and
manner of gene expression
3. Gene expression is the process by which
information from a gene is used in the synthesis of
a functional gene product.
These products are often proteins, but in non-
protein coding genes such as transfer RNA (tRNA)
or small nuclear RNA (snRNA) genes, the product
is a functional RNA.
Gene expression
4. How genes are expressed
The process of gene
expression involves two main
stages:
Transcription: the production
of messenger RNA (mRNA) by
the enzyme RNA polymerase,
and the processing of the
resulting mRNA molecule.
Translation: the use of mRNA
to direct protein synthesis
5. Gene control regions
A promoter. A region a few hundred nucleotides 'upstream' of
the gene (toward the 5' end).
It is not transcribed into mRNA, but plays a role in controlling
the transcription of the gene.
Transcription factors bind to the promoter region and assist in
the binding of RNA polymerases.
Enhancers. Some transcription factors (called activators)
bind to regions called 'enhancers' that increase the rate of
transcription
Silencers. Some transcription factors (called repressors)
bind to regions called 'silencers' that depress the rate of
transcription.
6. Types of gene regulation
Gene regulation can be negative or positive
. In negative regulation, a repressor molecule
binds to the sliencer and terminates transcription.
In positive regulation, an activator interacts with
the RNA polymerase in the promoter region to
initiate transcription.
7. Necessity of Gene Regulation
Unicellular organisms
Depending on the needs of the body some genes have
to be transcribed whereas the rest have to be switched
off
Helps to adapt to the changes in environment
Multicellular organisms
Helps in differentiation of cells
Performance of various functions in the body
