Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
ION EXCHANGE CHROMATOGRAPHY
ByM.Vharshini
B.Sc. Bio Medical Science
Sri Ramachandra University
ION EXCHANGE CHROMATOGRAPHY
Ion-exchange chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger.
It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
Cations or Anions can be separated using this method.
PRINCIPLE
It is based on the reversible electrostatic interaction of ions with the separation matrix (i.e.)
The separation occurs by reversible exchange of ions between the ions present in the solution and those present in the ion exchange resin.
CLASSIFICATION OF RESINS
According to the chemical nature they classified as-
1. Strong cation exchange resin
2. Weak cation exchange resin
3. Strong anion exchange resin
4. Weak anion exchange resin
According to the Source they can -
Natural resins : Cation - Zeolytes, Clay
Anion - Dolomite
Synthetic resins: Inorganic & Organic resins
◘Organic resins are polymeric resin matrix.
The resin composed of –
Polystyrene (sites for exchangeable functional groups)
Divinyl benzene(Cross linking agent)-offers stability.
Ion exchange resin should have following requirements
»It must be chemically stable.
»It should be insoluble in common solvents.
» It should have a sufficient degree of cross linking.
»The swollen resin must be denser than water.
»It must contain sufficient no. of ion exchange groups.
Physical properties of ion exchange resins
Cross linking:
It affects swelling & strength & solubility
Swelling:
When resin swells, polymer chain spreads apart
Polar solvents → swelling
Non-polar solvents → contraction
Swelling also affected electrolyte concentration.
Particle size and porosity
Increase in surface area & decrease in particle size will increase the rate of ion exchange.
Regeneration
Cation exchange resin are regenerated by treatment with acid, then washing with water.
Anion exchange resin are regenerated by treatment with NaOH, then washing with water until neutral.
EXPERIMENTAL SETUP OF ION EXCHANGE CHROMATOGRAPHY
Metrohm 850 Ion chromatography system
Instrumentation of ion exchange chromatography
PRACTICAL REQUIREMENTS
1.Column
» glass, stainless steel or polymers
2.Packing the column
» Wet packing method:
A slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. Care must be taken to avoid air bubbles.
3.Application of the sample
After packing, sample is added to the top of the stationary phase, use syringe or pipette.
This layer is usually topped with a small layer of sand or with cotton or glass wool to protect the shape of the organic layer from the velocity of newly added eluent.
4.Mobile phase
Acids, alkalis, buffers…
6.Stationary phase
The ionic
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
HPLC Principle,Instrumentation and ApplicationAlakesh Pradhan
HPLC Chromatography and its principle
Liquid chromatography
High Performance Liquid Chromatography ( HPLC )
The components of the high performance liquid chromatograph (HPLC).
The separation process.
The chromatogram
Spectroscopy techniques, it's principle, types and applications NizadSultana
Spectroscopy and it's applications as well as it's types like Infrared spectroscopy and ultraviolet spectroscopy and principle of spectroscopy why we use spectroscopy.
principle, application and instrumentation of UV- visible Spectrophotometer Ayetenew Abita Desa
This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. It covers beer-lambert low and its quantitative applications. It also includes the qualitative applications in different fields of study. Presented at Addis Ababa University, School of medicine, department of medical biochemistry.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
ION EXCHANGE CHROMATOGRAPHY
ByM.Vharshini
B.Sc. Bio Medical Science
Sri Ramachandra University
ION EXCHANGE CHROMATOGRAPHY
Ion-exchange chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger.
It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
Cations or Anions can be separated using this method.
PRINCIPLE
It is based on the reversible electrostatic interaction of ions with the separation matrix (i.e.)
The separation occurs by reversible exchange of ions between the ions present in the solution and those present in the ion exchange resin.
CLASSIFICATION OF RESINS
According to the chemical nature they classified as-
1. Strong cation exchange resin
2. Weak cation exchange resin
3. Strong anion exchange resin
4. Weak anion exchange resin
According to the Source they can -
Natural resins : Cation - Zeolytes, Clay
Anion - Dolomite
Synthetic resins: Inorganic & Organic resins
◘Organic resins are polymeric resin matrix.
The resin composed of –
Polystyrene (sites for exchangeable functional groups)
Divinyl benzene(Cross linking agent)-offers stability.
Ion exchange resin should have following requirements
»It must be chemically stable.
»It should be insoluble in common solvents.
» It should have a sufficient degree of cross linking.
»The swollen resin must be denser than water.
»It must contain sufficient no. of ion exchange groups.
Physical properties of ion exchange resins
Cross linking:
It affects swelling & strength & solubility
Swelling:
When resin swells, polymer chain spreads apart
Polar solvents → swelling
Non-polar solvents → contraction
Swelling also affected electrolyte concentration.
Particle size and porosity
Increase in surface area & decrease in particle size will increase the rate of ion exchange.
Regeneration
Cation exchange resin are regenerated by treatment with acid, then washing with water.
Anion exchange resin are regenerated by treatment with NaOH, then washing with water until neutral.
EXPERIMENTAL SETUP OF ION EXCHANGE CHROMATOGRAPHY
Metrohm 850 Ion chromatography system
Instrumentation of ion exchange chromatography
PRACTICAL REQUIREMENTS
1.Column
» glass, stainless steel or polymers
2.Packing the column
» Wet packing method:
A slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. Care must be taken to avoid air bubbles.
3.Application of the sample
After packing, sample is added to the top of the stationary phase, use syringe or pipette.
This layer is usually topped with a small layer of sand or with cotton or glass wool to protect the shape of the organic layer from the velocity of newly added eluent.
4.Mobile phase
Acids, alkalis, buffers…
6.Stationary phase
The ionic
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
HPLC Principle,Instrumentation and ApplicationAlakesh Pradhan
HPLC Chromatography and its principle
Liquid chromatography
High Performance Liquid Chromatography ( HPLC )
The components of the high performance liquid chromatograph (HPLC).
The separation process.
The chromatogram
Spectroscopy techniques, it's principle, types and applications NizadSultana
Spectroscopy and it's applications as well as it's types like Infrared spectroscopy and ultraviolet spectroscopy and principle of spectroscopy why we use spectroscopy.
principle, application and instrumentation of UV- visible Spectrophotometer Ayetenew Abita Desa
This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. It covers beer-lambert low and its quantitative applications. It also includes the qualitative applications in different fields of study. Presented at Addis Ababa University, School of medicine, department of medical biochemistry.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Gas chromatography–mass spectrometry (GC-MS) is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances within a test sample.[1] Applications of GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, food and flavor analysis, and identification of unknown samples, including that of material samples obtained from planet Mars during probe missions as early as the 1970s. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification. Like liquid chromatography–mass spectrometry, it allows analysis and detection even of tiny amounts of a substance.[2]
GC-MS has been regarded as a "gold standard" for forensic substance identification because it is used to perform a 100% specific test, which positively identifies the presence of a particular substance. A nonspecific test merely indicates that any of several in a category of substances is present. Although a nonspecific test could statistically suggest the identity of the substance, this could lead to false positive identification. However, the high temperatures (300°C) used in the GC-MS injection port (and oven) can result in thermal degradation of injected molecules,[3] thus resulting in the measurement of degradation products instead of the actual molecule(s) of interest.The first on-line coupling of gas chromatography to a mass spectrometer was reported in the late 1950s.[4][5] An interest in coupling the methods had been suggested as early as December 1954.
Gas chromatography-Mass spectrometry (GC-MS)Saira Fatima
PRESENTED BY
SAIRA FATIMA
SABAHAT MEHMOOD
SANA USMAN
MSc 4 (2018-2020)
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
Low amount of sample
Complex mixture.
Gas chromatography is a process of separating component(s) from the given crude drug or mixture by using stationary phase (solid or liquid) and gaseous mobile phase. It involves a sample being vaporized and injected onto the head of the chromatographic
column. The sample is transported through the column by the flow of inert, gaseous
mobile phase. The column itself contains a solid or liquid stationary phase which is adsorbed onto the
surface of an inert solid.
Gas Chromatography in Analytical Analysis.pptxRAHUL PAL
Gas chromatography is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture.
Human life is based upon the principle of work.
▪ One has to work to earn his livelihood.
▪ Pharmacy is one of the professions.
▪ The pharmacy council of India has introduced a new subject
named “Drug store and business management”.
▪ Syllabus is divided into two parts – part I commerce and part
II Accountancy.
▪ The purpose of this subject is to familiarise the students with
the basic concept of business, its proper management, sources
of finances in order to run it successfully and the last, the way
and means to note down various transactions in books of
account with a view to having a permanent record of the same.
Distribution is significant rated as a significant function of marketing. After
production a product moves to the market and finally to the consumer.
▪ This journey of the product from the manufacturer or producer to the consumer is
made possible through certain defined paths, termed as, “Channels of
Distribution”
Distribution is significant rated as a significant function of marketing. After
production a product moves to the market and finally to the consumer.
▪ This journey of the product from the manufacturer or producer to the consumer is
made possible through certain defined paths, termed as, “Channels of
Distribution”
Monoclonal antibodies are antibodies that have a high degree of specificity (mono-specificity) for an antigen or epitope. Monoclonal antibodies are typically derived from a clonal expansion of antibody producing malignant human plasma cells. The initial monoclonal antibodies were created by fusing spleen cells from an immunized mouse with human or mouse myeloma cells (malignant self-perpetuating antibody producing cells), and selecting out and cloning the hybrid cells (hybridomas) that produced the desired antibody reactivity. These initial monoclonal products were mouse antibodies and were very valuable in laboratory and animal research and diagnostic assays, but were problematic as therapeutic agents because of immune reactions to the foreign mouse protein. Subsequently, production of chimeric mouse-human monoclonal antibodies and means of further “humanizing” them and producing fully human recombinant monoclonal antibodies were developed.
Chapter-1 Introduction to Human Anatomy and PhysiologyD.R. Chandravanshi
Anatomy (Greek anatomē, 'dissection') is the branch of biology concerned with the study of the structure of organisms and their parts. Anatomy is a branch of natural science which deals with the structural organization of living things. It is an old science, having its beginnings in prehistoric times. Anatomy is inherently tied to developmental biology, embryology, comparative anatomy, evolutionary biology, and phylogeny, as these are the processes by which anatomy is generated, both over immediate and long-term timescales. Anatomy and physiology, which study the structure and function of organisms and their parts respectively, make a natural pair of related disciplines, and are often studied together. Human anatomy is one of the essential basic sciences that are applied in medicine.
Program among these measures are the NATIONAL HEALTH PROGRAMS, which have been launched by the central government of control/ eradication of communicable diseases, improvement of environmental sanitation, raising the standard of nutrition, control of population and improving rural health. Introduction
The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) is unique in bringing together the regulatory authorities and pharmaceutical industry to discuss scientific and technical aspects of drug registration. Since its inception in 1990, ICH has gradually evolved, to respond to the increasingly global face of drug development. ICH’s mission is to achieve greater harmonisation worldwide to ensure that safe, effective, and high quality medicines are developed and registered in the most resource-efficient manner. On 23 October 2015, ICH announced organisational changes as it marks 25 years of successful harmonisation.
Forms of business organization, DSBM D.Pharma 2nd yearD.R. Chandravanshi
The legal entity can be in any form of a business organization. The various forms of organization are as follows: 1) Sole proprietorship 2) Partnership 3) Co-operative Society 4) Joint stock company (Private and Public) These are explained in brief as follows:-3.1 OBJECTIVES At the end of this lesson you will be able to know z Various forms of organization z Its formation & features z Merits & Demerits
Psoriasis is an autoimmune condition that affects skin. It is characterized by changes in the skin that include hyperkeratosis, parakeratosis, and akantosis.
They are attributed to an increased mitosis rate in the basal region of the epidermis, as well as disorders of maturing and differentiating keratinocytes.
These changes in the dermis and epidermis cause the typical desquamation of the stratum corneum observed in psoriasis. The psoriatic lesions indicate an inflammatory reaction caused by the secretion of pro-inflammatory cytokines from macrophages, lymphocytes, and neutrophils.
These cytokines may stimulate the inflammatory response via the lipoxygenase and the cyclooxygenase (COX) pathways.
The red, scaling psoriatic plaques often itch and burn. People with psoriasis may suffer discomfort, including pain and itching and emotional distress Psoriasis affects 1% to 2% of the population.
Omega−3 fatty acids, also called Omega-3
oils, ω−3 fatty acids or n−3 fatty acids,
are polyunsaturated fatty acids (PUFAs)
characterized by the presence of a double
bond three atoms away from the terminal
methyl group in their chemical structure.
They are widely distributed in nature, being
important constituents of animal lipid
metabolism, and they play an important
role in the human diet and in human
physiology.
https://www.slideshare.net/DauRamChandravanshi1
Pilot Plant:-
“Defined as a part of pharmaceutical industry where a lab scale formula is transformed into viable product by the development of liable practical procedure for manufacture”.
Scale-up:-
“The art of designing of prototype using the data obtained from the pilot plant model”
Optimum performance laminar chromatography (OPLC) is a pumped flow chromatography techniques that combine the user – friendly interface of HPLC with the capacity of flash chromatography and multidimensionally of TLC .
Optimum performance laminar chromatography (OPLC ) , in a contrast , is a pumped flow chromatography system that uses a planar 2D column format .
The multidimensionally capacity of OPLC is not limited to the separation technique alone , but also to the multitude of sample application and detection methods that are available .
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
vaccine is a biological preparation that provides active acquired immunity to a particular disease. A vaccine typically contains an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe, its toxins, or one of its surface proteins. The agent stimulates the body's immune system to recognize the agent as a threat, destroy it, and to further recognize and destroy any of the microorganisms associated with that agent that it may encounter in the future.
HISTORY OF VACCINES-
EDWARD JENNER conduct experiments in 1796 that lead to the creation of the first smallpox vaccine for prevention of smallpox.
A vaccine for RABIES is developed by LOUIS PASTEUR .
Vaccine for COLERA and TYPHOID were developed in 1896 and PLAGE vaccine in 1887.
The first DIPHTHERIA vaccine is developed in about 1913 by EMIL ADOLPH BEHRING,WILLIAM HALLOCK PARK.
The whole cell PERTUSIS vaccines are developed in 1914.
A TETANUS vaccine is developed in 1927.
Immunity
It can be defined as the resistance to disease, specifically to infectious disease or pathogens. The term “immune” is derived from the Latin word “immunis” that is exempt from charges. In medical term, it refers to the being protected from infectious pathogens.
Immune system
It is adaptive defense system which is able to generate a variety of cell and molecules capable of specifically recognizing and eliminating a variety of limitless foreign invaders into the system.
In 1975 Georges Kohler and Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas that could produce antibodies.
antibody
Also known as immunoglobulin is a large, Y shaped glycoprotein produced mainly by plasma cells that is used by the immune system to neutralize pathogens.
monoclonal antibodies
Antibodies that are made by identical immune cells that are clones of a unique parent cell.
polyclonal antibodies
A polyclonal antibodies represents a collection of antibodies from different B cells that recognize multiple epitopes on the same antigen.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition.
SEMs can magnify an object from about 10 times up to 300,000 times. A scale bar is often provided on an SEM image. From this the actual size of structures in the image can be calculated.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
Parenterals are the sterile preparation that is directly administered into the circulatory system avoiding the enteral route. And these preparation provide rapid onset of action that is why the administered preparation must be safe.
Stability problem arise from microbial contamination of these products so sterility and stability must be ensured for these preparations.
To ensure their sterility and stability, regulations regarding to quality control through pharmacopeial specifications has great importance.
Factors affecting Design of Experiment (DOE) and softwares of DOED.R. Chandravanshi
What is an experiment ?
An experiment refers to any process that generates a set of data.
An experiment involves a test or series of test in which purposeful changes are made to the input variables of a process or system so that changes in the output responses can be observed and identified.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
1. Presented By-
Sudipta Nandi
M. Pharm 1st Sem
Department of Pharmaceutical Sciences
Dr. Harisingh Gour Vishwavidyalaya Sagar, M.P.
(A Central University)
2. Content
INTRODUCTION
HISTORICAL BACKGROUND OF GC-MS
PRINCIPLE
INSTRUMENTATION
• GAS CHROMATOGRAPHY
• INTERFACE
• MASS SPECTROMETER
WORKING
LIMITATIONS
APPLICATION
CONCLUSION
REFERENCES
3. INTRODUCTION
Gas chromatography-mass spectrometry (GC-MS) is the synergistic
combination of two analytical method to separate and identify different
substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and
structural elucidation of each component.
HYPHENATED
TECHNIQUES
5. • Roland Gohlke and Fred mcLafferty introduce use of MS as
detector of GC in 1950s.
• Miniaturized computers has helped in the simplification of
instrument.
• In 1968, the Finnigan Instrument Corporation delivered the first
quadrupole GC-MS.
• By the 2000s computerized GC-MS instruments using quadrupole
technology had become essential.
• In 2005 GC tandem MS-MS have been introduced.
6. The sample solution is injected into the GC inlet where it is
vaporized and swept onto a chromatographic column by the
carrier gas.
The sample flows through column and compounds are
separated by virtue of their relative interaction with the
coating of the column and the carrier gas.
Latter part of column passes through a heated transfer line
and ends at entrance to ion source where compounds eluting
from the column are converted to ions and detected
according to their mass to charge m/z ratio.
7. INSTRUMENTATION LAYOUT
Gas
chromatography
• Carrier gas
• Pneumatic control
• Injector
• Column
• Oven
Interface
• Jet Interface
• Direct Capillary
Infusion Interface
• Watson-Biemann
effusion separator
Mass
spectrometer
• Ion source
• High-vacuum
system
• Mass analyzer
• Ion collector
Data
system
Fig : Principle of GC-MS
8.
9. 1. Carrier Gas :
Served as mobile phase supplied in the steel tank under high pressure.
At pressure of 40-80 psi this passes into flow controllers.
Example- nitrogen, helium; hydrogen and argon can also be used.
Requirements –
Inert
Column requirements
Detectors
Purity- better than 99.995%
Cost effective & available
10. 2. Pneumatic Control :
Gas supply is regulated to the correct pressure and then fed to the required
part of instrument.
Older instruments – manual pressure control via regulators.
Modern GC instruments – Electronic Pneumatic pressure controller.
3. Oven :
Temperature programmable, typically range from 5°C - 400°C but can go as
low as -25°C with cryogenic cooling.
11. 4. Sample Injection Port :
Sample is made to vaporized rapidly before entering to column.
Various kind of injectors :
Packed column injectors
Split injection
Splitless injection
Programmed Split/Splitless injection
Programmed On-Column injector
5. Column :
Two kinds of column used :
Packed column
Capillary column
Gas chromatograph GC-MS utilizes capillary column.
Here, stationary phase has been chemically bonded to the fused silica, e.g.,
DB-5.
12. Packed Column :-
• Less commonly used, having
diameter of 2-3 mm and length of
0.5-10 m.
• Manufactured from steel or glass,
internal wall is treated to avoid
catalytic effect with the sample.
• They can withstand a carrier gas
flow rate within range 10-
40ml/min.
Capillary Column :-
• Consist of long capillary tubing
10-100 m in length.
• Made up from stainless steel &
coil.
• Shorter columns - for fast analysis
• Larger columns – for high
resolution separation
13. 6. Detectors :
Simple and reliable
Sensitive to electronegative groups (halogens)
Largely non-destructive
Limited dynamic range (10²)
Mass sensitive detectors
-Thermal Conductivity Detector (TCD)
-Flame Ionization Detector (FID)
-Electron Capture Detector (ECD)
14. INTERFACE
Pressure incompatibility problem between GC and MS was solved by
inserting an interface.
Mainly three types of interfaces are there commercially available :
Jet/Orifice separator
Direct capillary infusion interface
Watson-Biemann effusion separator
Fig : Direct capillary infusion
Fig : Jet separator Fig : Watson-Biemann effusion
15. MASS SPECTROMETER
“ Mass spectrometry is a technique used for measuring the molecular weight
and determining the molecular formula of an organic compound.”
In general a mass spectrometer consists of
An ion source
High-vacuum system
A mass-selective analyzer
An ion collector
Data system
16. Ionization Method Typical Analytes Sample
Introduction
Mass
Range
Method
Highlights
Chemical Ionization (CI) Relatively small,
volatile
GC or
liquid/solid
probe
Upto
1000
Daltons
Soft method,
molecular ion peak
[M+H] +
Electron Impact
Ionization (EI)
Relatively small,
volatile
GC or
liquid/solid
probe
Upto
1000
Daltons
Hard method,
versatile, provides
structure info
Electrospray Ionization
(ESI)
Peptides, proteins,
nonvolatile
Liquid
chromatography
Upto
200000
Daltons
Soft method, ions
often multiply
charged
Fast Atom Bombardment
(FAB)
Carbohydrates,
organometallics,
peptides,
nonvolatile
Sample mixed in
viscous matrix
Upto
6000
Daltons
Harder than ESI or
MALDI
Matrix Assisted Laser
Desorption Ionization
(MALDI)
Peptides, proteins,
nucleotides
Sample mixed in
solid matrix
Upto
500000
Daltons
Soft method, very
high mass
17. Mass Selective Analyzer
• They deflects ions down a curved tubes in a magnetic fields based on their
kinetic energy determined by the mass, charge and velocity.
• The magnetic field is scanned to measure different ions.
Mass Analyzers
Quadrupole Ion Trap Time of flight
18. Quadrupole Analyzer :
• Also known as ‘Hewlett-Packard’ or ‘Mass Selective Detector’.
• In quadrupole mass analyser a set of four rods are arranged parallel to the
direction.
• Only m/z is been determined and stable oscillation takes place.
• Ions travels in quadrupole axis with cork screw type of trajectory.
• It functions as a mass filter
19. Ion Trap Mass Analyzer :
The ion trap mass analyzer operates by similar principles where it consists
of circular ring electrode and two end caps that form a chamber.
AC or DC power along RF potential is applied between the cups and the
ring electrode.
Ions entering into
chamber
Trapped by
electromagnetic fields
Ions oscillate in concentric
trajectories
Resonant Ejection
20. Time-of-flight Analyzer :
The time-of-flight (TOF) analyser uses an electric field to accelerate the
ions through the same potential.
Then it measures the time take to reach the detector.
If the particles all have the same charge, the kinetic energies will be
identical and their velocities will depend upon only on their masses.
21. Mass spectrometers employ computer control of same functions and also
use a computerised display and output.
It is used to identify and measure the concentration of one or more analytes
in a complex mixture
Advantages –
Amount of data generated is very large
It stores every sec for upto 90 min.
Data Handling
MASS
CHROMATOGRAMS
SELECTED ION
MONITORING
22. GC TRACE OF A THREE COMPONENT MIXTURE.
THE MASS SPECTROMETER GIVES A SPECTRUM FOR
EACH COMPONENT
24. LIMITATIONS
• Only compounds with vapour pressure exceeding about 10-10 torr can be
analysed by gas chromatography-mass spectrometry (GC-MS).
• Determining positional substitution on aromatic ring is often difficult.
• Certain isomeric compounds cannot be distinguished by mass spectrometry.
• Non-volatile matrices require additional preparation (extraction,
outgassing, etc.)
• Atmospheric gases are challenging (CO2, N2, O2, Ar, CO, H2O).
25. Applications of GC-MS
Petrochemical and hydrocarbon analysis
Geochemical research
Forensic (arsons, explosives, drugs, unknowns)
Environmental analysis
Pesticide analysis, food safety and quality
Clinical toxicology
Food, beverage and perfume analysis
26. CONCLUSION
As we can see, GC-MS is used both in qualitative and quantitative
measurement.
Being hyphenated technique, the cost of GC-MS becomes very high.
Nowadays, cost is also cheaper than before as quadrupole and ion trap
instruments are used, which does not only save space but also save
manufacturing cost.
GC-MS is an analytical method of increased sensitivity and reliability even
in very small quantities (ng).
27. Braun R., Introduction To Instrumental Analysis, Second
Edition, PharmaMed Press, Hyderabad, Page no. 251-270.
Chatwal G.R., Anand S.K., Instrumental method of Chemical
Analysis, Himalaya Publishing House, Fifth Edition-2012,
New Delhi, Page no. 420-449.
Gohlke R.S.(1959), Analytical Chemistry, 36, Page no. 759-
764.