Energy-rich or high-energy compounds in biological systems are those whose hydrolysis yields a free energy (ΔG0') of greater than -25 kJ/mol. These compounds contain high-energy bonds like phosphoanhydride, enol phosphate, acyl phosphate, and guanido phosphate bonds. ATP is an important energy-rich compound that occupies an intermediate position with a ΔG0' of -7.3 kcal/mol for hydrolysis. Its intermediate energy level allows it to accept high-energy phosphoryl groups from compounds like phosphoenolpyruvate and pass them to lower energy compounds. This enables ATP to act as an efficient universal energy carrier in cells.
A Remarkable Conflict Between Biochemical Dogma And Radical Concept :
Traditional Concept :
By the 1950s it had been clearly established that oxidative phosphorylation involved the stepwise transfer of electrons through a series of carriers to molecular oxygen. But how the energy derived from these electron transfer reactions was converted to ATP remained a mystery. The general assumption was that ADP was converted to ATP by direct transfer of high-energy phosphate groups from some other intermediate. Thus it was postulated that high-energy intermediates were produced as a result of electron transfer reactions and that these intermediates drove ATP synthesis by phosphate group transfer.
Concept Proposed By Peter Mitchell :
The fundamental proposal of the chemiosmotic hypothesis was that the “intermediate” that coupled electron transport to ATP synthesis was a proton electrochemical gradient across the membrane. Mitchell postulated that such a gradient was produced by electron transport and that the flow of protons back across the membrane in the energetically favorable direction was then coupled to ATP synthesis
Enzymalogy Factors affecting enzyme activity and kineticsrohini sane
A comprehensive presentation on Factors affecting enzyme activity & Kinetics of Enzymes for MBBS ,BDS, B Pharm & Biotechnology students to facilitate self- study.
Biological oxidation (part - III) Oxidative PhosphorylationAshok Katta
Biological oxidation (part - III) Oxidative Phosphorylation
- Mechanism of Oxidative Phosphorylation
-- Chemiosmotic theory
-P:O Ratio
Substrate Level Phosphorylation
Shuttle Systems for Oxidation of Extramitochondrial NADH
A Remarkable Conflict Between Biochemical Dogma And Radical Concept :
Traditional Concept :
By the 1950s it had been clearly established that oxidative phosphorylation involved the stepwise transfer of electrons through a series of carriers to molecular oxygen. But how the energy derived from these electron transfer reactions was converted to ATP remained a mystery. The general assumption was that ADP was converted to ATP by direct transfer of high-energy phosphate groups from some other intermediate. Thus it was postulated that high-energy intermediates were produced as a result of electron transfer reactions and that these intermediates drove ATP synthesis by phosphate group transfer.
Concept Proposed By Peter Mitchell :
The fundamental proposal of the chemiosmotic hypothesis was that the “intermediate” that coupled electron transport to ATP synthesis was a proton electrochemical gradient across the membrane. Mitchell postulated that such a gradient was produced by electron transport and that the flow of protons back across the membrane in the energetically favorable direction was then coupled to ATP synthesis
Enzymalogy Factors affecting enzyme activity and kineticsrohini sane
A comprehensive presentation on Factors affecting enzyme activity & Kinetics of Enzymes for MBBS ,BDS, B Pharm & Biotechnology students to facilitate self- study.
Biological oxidation (part - III) Oxidative PhosphorylationAshok Katta
Biological oxidation (part - III) Oxidative Phosphorylation
- Mechanism of Oxidative Phosphorylation
-- Chemiosmotic theory
-P:O Ratio
Substrate Level Phosphorylation
Shuttle Systems for Oxidation of Extramitochondrial NADH
Cellular Energy Transfer (Glycolysis and Krebs Cycle) and ATPmuhammad aleem ijaz
This presentation is all about Cellular Energy Transfer with reference to Glycolysis and Kreb Cycle with all their stages involved.
It also includes ATP production in the body, its importance, structure.
Also contains a comparison of energy production in Krebs and Glycolysis cycle.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
CDSCO and Phamacovigilance {Regulatory body in India}NEHA GUPTA
The Central Drugs Standard Control Organization (CDSCO) is India's national regulatory body for pharmaceuticals and medical devices. Operating under the Directorate General of Health Services, Ministry of Health & Family Welfare, Government of India, the CDSCO is responsible for approving new drugs, conducting clinical trials, setting standards for drugs, controlling the quality of imported drugs, and coordinating the activities of State Drug Control Organizations by providing expert advice.
Pharmacovigilance, on the other hand, is the science and activities related to the detection, assessment, understanding, and prevention of adverse effects or any other drug-related problems. The primary aim of pharmacovigilance is to ensure the safety and efficacy of medicines, thereby protecting public health.
In India, pharmacovigilance activities are monitored by the Pharmacovigilance Programme of India (PvPI), which works closely with CDSCO to collect, analyze, and act upon data regarding adverse drug reactions (ADRs). Together, they play a critical role in ensuring that the benefits of drugs outweigh their risks, maintaining high standards of patient safety, and promoting the rational use of medicines.
The Gram stain is a fundamental technique in microbiology used to classify bacteria based on their cell wall structure. It provides a quick and simple method to distinguish between Gram-positive and Gram-negative bacteria, which have different susceptibilities to antibiotics
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
2. • Energy-rich compounds: Such compounds in biological system whose ΔG0’ of hydrolysis yields free energy equal to or greater
than -25 kJ/mol are called energy-rich compounds or high-energy compounds, while, those whose ΔG0’ of hydrolysis yields
free less than -25 kJ/mol are called low-energy compounds.
• Most of the high-energy compounds contain phosphate group (viz., phosphoanhydride, enol phosphates, acyl phosphates and
guanido phosphates) are also called high-energy phosphates having high phophoryl transfer potential except thiol esters that
have high acyl transfer potential.
• High-energy bonds: The bonds in the energy-rich compounds, which yields high energy upon hydrolysis are called high-
energy bonds. A squiggle ‘~’ is used to indicate energy-rich bonds. In 1941 Fritz Lipmann introduced this convention.
• The term "high-energy bond," is however incorrect and misleading because it wrongly suggests that the bond itself contains
the energy. The fact is that breaking of chemical bonds requires an input of energy. The free energy released by hydrolysis of
high-energy compounds results from the products of the reaction having a smaller free-energy content than the reactants and
that’s not from breaking of any specific bond.
• ΔG⁰` - It is the difference in free energies of the products and reactants.
Energy Rich Compounds
3. S. No.
Energy-rich
compounds
Energy-rich bond Example
ΔG⁰`
(kJ/mol) (kcal/mol)
1 Pyrophosphates
Acid anhydride
(formed by condensation of acid groups or its
derivatives)
– C–P~P ATP to ADP -30.5 -7.3
2 Enol phosphates
Enol phosphate
(It is formed when phosphate group attaches to a
hydroxyl group which is bounded to a carbon atom
having double bond.)
– CH
║
– C–O~P
Phosphoenol pyruvate -61.9 -14.8
3 Acyl phosphates
Bond between carboxy and phosphate
(The high energy bond in this compound is formed by
the reaction between carboxylic acid group and
phosphate group)
O
║
– C–O~P
1,3-
bisphosphoglycerate
-49.4 -11.8
4 Thiol esters
Thioester
(Thioester bond results from the reaction between thiol
and carboxylic acid group)
O
║
– C~S –
Acetyl CoA -31.4 -7.5
5
Guanido
phosphates
(phophagens)
Guanidine phosphate bond
(It is formed by the attachment of phosphate group to
guanidine group)
|
– N~P Phosphocreatine -43.1 -10.3
Classification of Energy-rich compounds: Based on type of bonds
4. Structure of some energy-rich compounds
1,3-bisphosphoglycerate
Acetyl CoA
Phosphoenol phosphate
Phosphocreatine
ATP
~
~
O
~
~ ~
~ ~
~
-
5. What makes these compounds energy-rich?
Resonance stabilization, solvation and/or electrostatic repulsion
Let’s have a look at the structure of individual energy-rich compound and their
hydrolytic product…
6. Adenosine triphosphate (ATP)
• ATP is a nucleotide made up of adenine, ribose sugar and a triphosphate group (viz., α, β and γ designated from
C5’).
• Adenine is linked to C1’ of sugar via N-glycosidic bond.
• α- phosphate is attached to C’5 of sugar via phosphoester bond, while β- and γ- phosphate via phosphoanhydride
bond.
• It is a energy rich compound because it contains 2 energy-rich phosphoanhydride bonds, one between α- and β-,
while second between β- and γ- phosphoryl groups.
•
γ β α
Adenine
Ribose sugar
Triphosphate groups
N-glycosidic bond
Phosphoanhydride bond Phosphoester bond
Fig.1. Structure of ATP
9
1’
5’
4’
3’ 2’
4
3
2
1
5
6
7
8
Adenosine triphosphate (ATP)
~ ~
7. • ATP hydrolysis is highly exergonic i.e., a large amount of free energy is liberated when ATP is hydrolyzed to
adenosine diphosphate (ADP) and orthophosphate (Pi) or when ATP is hydrolyzed to adenosine
monophosphate (AMP) and pyrophosphate (PPi ).
• While, the synthesis of ATP by phosphorylation of ADP is a highly endergonic reaction.
ATP4¯ + H2O ADP 3¯ + Pi2¯ + H +
ΔG⁰` = (+7.3 kcal/mol)
ATP4¯ + H2O AMP2¯+ PPi3¯ + H +
ΔG⁰` = (-10.9 kcal/mol)
ATP hydrolysis is highly exergonic
ΔG⁰` = -7.3 kcal/mol
• Since ΔG⁰` is the difference of free energy of reactant (ATP) and product (ADP & Pi), so structure of both need to
be examined to understand this.
• 3 factors responsible for making ATP hydrolysis exergonic i.e. high phosphoryl transfer potential are: 1. Resonance
stabilization 2. Electrostatic repulsion 3. Stabilization due to hydration
What makes ATP hydrolysis exergonic?
ΔG⁰` = (+10.9 kcal/mol)
8. 1. Resonance stabilization:
•Products (ADP and Pi) has greater resonance stabilization than that of reactant (ATP).
•Pi has a number of resonance forms of similar energy, whereas the γ- phosphoryl group of ATP has a smaller number.
Fig.2B. Improbable resonance structure of terminal part of ATP
AMP
γ
β
B.
A.
cont.
Fig.2A. Resonance structure of orthophosphate (Pi)
9. 2. Electrostatic repulsion:
• Improbable resonance structure of terminal part of ATP (Fig. 2B) is unfavorable because a +vely charged O-atom is
adjacent to a +vely charged P-atom, an electrostatically unfavorable juxtaposition.
• ATP carries 4 –ve charges in close proximity (Fig 1.) at neutral pH leading to electrostatic charge repulsion. Release
of the terminal phosphate group removes an unfavorable repulsion between adjacent negative charges. This
repulsion is reduced by hydroxylation.
3. Stabilization due to hydration:
• Water can bind more effectively to ADP and Pi than it can to the phosphoanhydride part of ATP, stabilizing the ADP
and Pi by hydration.
• In other words solvation of products (ADP and Pi ) or (AMP and PPi ) is better than solvation of ATP.
cont.
10. Phosphoenol pyruvate (PEP)
•Reasons that favors hydrolysis of PEP to pyruvate:
•Electrostatic charge repulsion between negatively charged oxygen of phosphoryl group and carboxyl group favors removal of Pi.
•Resonance stabilization of hydrolytic product Pi.
•Isomerization (tautomerization) of hydrolytic product pyruvate into enol- keto form.
11. 1,3-Bisphosphoglycerate (1,3-BPA)
•Reasons that favors hydrolysis of 1,3-BGA to 3-Phosphoglyceraldehyde:
•Resonance stabilization of hydrolytic product Pi.
• Release of proton from hydrolytic product 3-phosphoglyceric acid to maintain pH of cell.
•Resonance stabilization of carboxylic group of 3-phosphoglycerate.
12. Acetyl CoA
•Reasons that favors hydrolysis of phosphocreatine to creatine: Hydrolytic removal of CoA from Acetyl CoA favors ionization
and resonance stabilization of carboxylic group of the hydrolytic product.
13. Phosphocreatine
•Reasons that favors hydrolysis of phosphocreatine to creatine:
•Resonance stabilization of hydrolytic product Pi.
•Resonance stabilization of hydrolytic product creatine.
~
~
14. ATP : UNIVERSAL ENERGY COULER
Intermediate position in phosphate-rich compounds
15. • Phosphate transfer potential: It is defined as the negative of the standard free energy change (ΔG0’) for the hydrolytic
removal of phosphate from the phosphorylated compounds.
• Phosphates can be transferred from compounds with higher ΔG0’. to those with lower ΔG0’.
• ATP occupies an intermediate position in overall spectrum of energy-rich phosphorylated compounds (i.e., compounds
with high phosphoryl transfer potential (Table 1).
• Compounds viz., phosphoenolpyruvate (PEP), 1,3-bisphosphoglycerate (1,3-BPG), and creatine phosphate have a higher
phosphoryl-transfer potential than that of ATP, while glucose-6-phosphate, glycerol-3-phosphate, etc. have low potential.
Phosphoryl-transfer potential : Intermediate position of ATP
Phosphorylated Compound ΔG0‘ (kcal/mol)
Phosphoenolpyruvate -14.8
1,3-bisphosphoglycerate -11.8
Creatine phosphate -10.3
ATP (to ADP) -7.3
Glucose-1-phosphate -5.0
Pyrophosphate -4.6
Glucose-6-phosphate -3.3
Glycerol-3-phosphate -2.2
Table1. Standard free energy of some phosphorylated compound
High
Energy
Comps
Low
Energy
comps
16. • This intermediate position ATP makes it an efficient carrier of phosphoryl groups, where ATP can act as a phosphate donor
and ADP, its dephosphorylated form can act as a phosphate acceptor linking energy producing and energy requiring reactions.
• Compounds possessing high-phosphoryl-transfer-potential (1,3-BPG, PEP, and creatine phosphate) derived from the metabolism
of fuel molecules can transfer their phosphoryl group to ADP to form ATP. This mechanism for making ATP is called
substrate-level phosphorylation.
• During glycolysis PEP and 1,3-BPG transfer its phosphoryl group to ADP to form ATP.
• Creatine phosphate serves as an energy reserve in vertebrate muscle. It is used to regenerate ATP from ADP every time we
exercise strenuously.
• In turn, ATP donates a phosphoryl group to form low energy compounds to facilitate their metabolism.
cont.
PEP + ADP + H+ Pyruvate + ATP
1,3-BPG + ADP+ H+ 3-phosphoglycerate + ATP
Phosphoglycerokinase
Pyruvatekinase
Phosphocreatine + ADP + H+ Creatine + ATP
Creatine kinase
Glucose + ATP Glucose-6-phosphate + ADP + H+
Hexokinase
17. Role of ATP/ADP System: An intermediate in cellular energy metabolism
• The free energy liberated during hydrolysis of ATP is harnessed to drive reactions that require an input of free
energy, such as muscle contraction. In turn, ATP is formed from ADP and Pi when fuel molecules are oxidized in
chemotrophs or when light is trapped by phototrophs. This ATP–ADP cycle is the fundamental mode of energy
exchange in biological systems.
• ATP is the major energy currency for cells, but it is not the only energy currency.
• Other nucleoside triphosphates (NTPs) have major roles in metabolism. Guanosine 5'-triphosphate (GTP) supplies
some of the energy used during protein synthesis. Cytidine 5'-triphosphate (CTP) is used during lipid synthesis, and
uridine 5'-triphosphate (UTP) is used for the synthesis of peptidoglycan and other polysaccharides.
Energy from Sunlight or food
i.e. phototrphy, chemotrphy,
arebic/anaerobic respiration,
fermentation
Energy available for cellular
work and for chemical synthesis
ATP
ADP + Pi
Fig. Cell’s energy cycle