This document discusses the biosynthesis of purines and pyrimidines. It explains that purines and pyrimidines are synthesized through de novo and salvage pathways. The de novo pathway involves multiple enzyme-catalyzed steps to convert simple precursors into the complex purine and pyrimidine nucleotides. This includes converting ribose-5-phosphate into inosine monophosphate (IMP) through 10 steps for purine synthesis. IMP is then used to synthesize adenine monophosphate (AMP) and guanine monophosphate (GMP). The salvage pathway recovers bases and nucleotides from degraded DNA and RNA. Pyrimidine synthesis is described as simpler than purine synthesis.
De novo and salvage pathway of nucleotides synthesis.pptx✨M.A kawish Ⓜ️
This slides explains Metabolism topic "De novo and salvage pathway of nucleotides synthesis. In which synthesis of Purines and pyrimidines synthesis has been occurred. In last there is a difference between these two pathways.
Nucleotide Biosynthesis involves 2 processes. one is Denovo synthesis and other is Salvage pathway. An outline of both the processes has given in this presentation.
De novo and salvage pathway of nucleotides synthesis.pptx✨M.A kawish Ⓜ️
This slides explains Metabolism topic "De novo and salvage pathway of nucleotides synthesis. In which synthesis of Purines and pyrimidines synthesis has been occurred. In last there is a difference between these two pathways.
Nucleotide Biosynthesis involves 2 processes. one is Denovo synthesis and other is Salvage pathway. An outline of both the processes has given in this presentation.
Pentose phosphate pathway is also called Hexose monophosphate pathway/ HMP shunt/ Phosphogluconate pathway.
It is an alternative route for the metabolism of glucose.
It is more complex pathway than glycolysis.
It is more anabolic in nature.
It takesplace in cytosol.
The tissues such as liver, adipose tissue, adrenal gland, erythrocytes,testes and lactating mammary gland are highly active in HMP shunt.
It concern with the biosynthesis of NADPH and pentoses.
Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry
*images use in this ppt is only for educational purpose
In this presentation, i tell about substrate level phosphorylation
Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)
Coenzyme - Introduction, Definition, Examples for coenzyme, reaction catalysed by coenzyme, Types of coenzymes - cosubstrate and prosthetic group coenzymes, second type of classification of coenzyme- hydrogen group transfer , other than hydrogen group transfer.
introduction of Purine and Pyrimidine metabolism, biosynthesis and degradation of nucleotides, biological functions and metabolic disorders, chemical analogues and therapeutic drugs, uric acid metabolism
explains the breakdown of purine. source and excretion of purine is explained. hyperuricemia and hypouricemia is discussed. types of Gout, clinical features and treatment is included.
Pentose phosphate pathway is also called Hexose monophosphate pathway/ HMP shunt/ Phosphogluconate pathway.
It is an alternative route for the metabolism of glucose.
It is more complex pathway than glycolysis.
It is more anabolic in nature.
It takesplace in cytosol.
The tissues such as liver, adipose tissue, adrenal gland, erythrocytes,testes and lactating mammary gland are highly active in HMP shunt.
It concern with the biosynthesis of NADPH and pentoses.
Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry
*images use in this ppt is only for educational purpose
In this presentation, i tell about substrate level phosphorylation
Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)
Coenzyme - Introduction, Definition, Examples for coenzyme, reaction catalysed by coenzyme, Types of coenzymes - cosubstrate and prosthetic group coenzymes, second type of classification of coenzyme- hydrogen group transfer , other than hydrogen group transfer.
introduction of Purine and Pyrimidine metabolism, biosynthesis and degradation of nucleotides, biological functions and metabolic disorders, chemical analogues and therapeutic drugs, uric acid metabolism
explains the breakdown of purine. source and excretion of purine is explained. hyperuricemia and hypouricemia is discussed. types of Gout, clinical features and treatment is included.
Biosynthesis of pyrimidine nucleotides can occur by a de novo pathway or by the reutilization of preformed pyrimidine bases or ribonucleosides (salvage pathway).
The pyrimidine synthesis is a similar process than that of purines. In the de novo synthesis of pyrimidines, the ring is synthesized first and then it is attached to a ribose-phosphate to for a pyrimidine nucleotide.
BIOSYNTHESIS OF PYRIMIDINES
SALVAGE PATHWAY
DE NOVO PATHWAY
SYNTHESIS OF OTHER PYRIMIDINE NUCLEOTIDES
IMPORTANCE
Essential building blocks of nucleic acids
Biologically very important heterocycles
Used in anti-biotics, used as anti-bacterial and anti-fungal also
Derivatives of pyrimidine also possess good anti-viral properties
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
2. Introduction
Biosynthesis is a multi-step, enzyme-catalyzed
process where substrates are converted into more
complex products in living organisms.
In biosynthesis, simple compounds are modified,
converted into other compounds, or joined together
to form macromolecules.
This process often consists of metabolic pathways.
The purines are built upon a pre-existing ribose 5-
phosphate.
Liver is the major site for purine nucleotide
synthesis.
Erythrocytes, polymorphonuclear leukocytes & brain
cannot produce purines.
3. Pathways
• There are Two pathways for the synthesis of
nucleotides:
1. De-novo synthesis: Biochemical pathway where
nucleotides are synthesized from new simple
precursor molecules
2. Salvage pathway: Used to recover bases and
nucleotides formed during the degradation of
RNA and DNA.
4.
5.
6.
7. Step involved in purine biosynthesis
(Adenine & Guanine)
• Ribose-5-phosphate, of carbohydrate metabolism
is the starting material for purine nucleotide
synthesis.
• It reacts with ATP to form phosphoribosyl
pyrophosphate (PRPP).
• Glutamine transfers its amide nitrogen to PRPP to
replace pyrophosphate & produce 5-
phosphoribosylamine. Catalysed by PRPP
glutamyl amidotransferase.
• This reaction is the committed.
8. • Phosphoribosylamine reacts with glycine in the
presence of ATP to form glycinamide ribosyl 5-
phosphate or glycinamide ribotide
(GAR).Catalyzed by synthetase.
• N10-Formyl tetrahydrofolate donates the formyl
group & the product formed is formylglycinamide
ribosyl 5-phosphate. Catalyzed by
formyltransferase.
• Glutamine transfers the second amido amino
group to produce formylglycinamidine ribosyl 5-
phosphate. Catalyzed by synthetase.
9. • The imidazole ring of the purine is closed in an
ATP dependent reaction to yield 5-
aminoimidazole ribosyl 5-phosphate. Catalyzed
by synthetase.
• Incorporation of CO2 (carboxylation) occurs to
yield aminoimidazole carboxylate ribosyl 5-
phosphate. Catalyzed by carboxylase.
• Does not require the vitamin biotin or ATP.
• Aspartate condenses with the aminoimidazole
carboxylate ribosyl 5- phosphate to form
aminoimidazole 4- succinylcarboxamide ribosyl
5-phosphate. Catalyzed by synthetase.
10. • Adenosuccinatelyase cleaves off fumarate & only
the amino group of aspartate is retained to yield
aminoimidazole 4-carboxamide ribosyl 5-
phosphate.
• N10-Formyl tetrahydrofolate donates one carbon
moiety to produce 5- formaminoimidazole 4-
carboxamide ribosyl 5- phosphate. Catalyzed by
formyltransferase.
• The final reaction catalyzed by cyclohydrolase
leads to ring closure with an elimination of water
molecule.
• The product obtained is Inosine Monophosphate
(IMP), the parent purine nucleotide from which
other purine nucleotides can be synthesized.
12. Synthesis of AMP & GMP from IMP
Synthesis of AMP:
Ionosine monophosphate (IMP) is the
immediate precursor for the formation
of AMP & GMP.
Aspartate condenses with IMP in the
presence of GTP to produce
adenylsuccinate which, on cleavage,
forms AMP.
13. Synthesis of GMP:
IMP undergoes NAD+ dependent
dehydrogenation to form xanthosine
monophosphate (XMP).
Glutamine then transfers amide nitrogen to
xanthosine monophosphate (XMP) to produce
GMP.
6-Mercaptopurine is an inhibitor of the
synthesis of AMP & GMP.
It acts on the enzyme adenylsuccinase (of AMP
pathway) & IMP dehydrogenase (of GMP
pathway).
16. Inhibitor of purine biosynthesis
• Folic acid (THF) is essential for the synthesis of
purine nucleotides.
• Sulfonamides are the structural analogs of Para-
aminobenzoic acid (PABA).
• These sulfa drugs can inhibit the synthesis of
folic acid by microorganisms. This indirectly
reduces the synthesis of purines & nucleic acids
(DNA & RNA).
• The structural analogs of folic acid (e.g.
methotrexate), used to control cancer.
17. • Azaserine (diazo acetyl-L-Serine) is a glutamine
antagonist & inhibits reactions involving
glutamine.
• Other synthetic nucleotide analogues used as
anticancer agents are 6-thio guanine & 8- aza
guanine.
18. Biosynthesis of pyrimidine
(Uracil, Cytosine & Thymine)
De novo synthesis of pymiridine:
• Biosynthesis of pyrimidine is simple than that of purine.
19.
20.
21.
22.
23.
24. Important links for study
• http://cancerres.aacrjournals.org/content/can
res/37/9/3080.full.pdf
• http://www.jbc.org/content/202/1/241.full.pd
f
• http://www.jbc.org/content/early/2018/01/0
3/jbc.M117.809459.full.pdf
• http://www.jbc.org/content/253/19/6794.full.
pdf