E. coli is a common Gram-negative bacteria that normally inhabits the gastrointestinal tract. Certain strains can cause illnesses such as gastroenteritis, urinary tract infections, and neonatal meningitis. E. coli is biochemically identified using tests such as IMViC, which examines indole production, methyl red, Voges-Proskauer, and citrate utilization. Treatment involves antibiotics such as ampicillin, but there is increasing antibiotic resistance in many strains. Preventing the spread of pathogenic E. coli requires proper sanitation and food handling.